Restriction map for
pF1KB5708
(Circular) (Six-base) MAPSORT of: pF1KB5708.seq Check: 883 from: 1 to: 5267
pF1KB5708 5267 bp
With 182 enzymes: SgfI *
April 6, 2017 11:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1359 1359
Size: 5267
AccI GT'mk_AC
Cuts at: 2305 2305
Size: 5267
Acc65I G'GTAC_C
Cuts at: 2283 2283
Size: 5267
AclI AA'CG_TT
Cuts at: 5107 5107
Size: 5267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 1212 1678 1865 1885 1991 2245 3092 3524
Size: 470 466 187 20 106 254 847 432
Cuts at: 3524 4271 4539 742
Size: 747 268 1470
Fragments arranged by size:
1470 847 747 470 466 432 268 254
187 106 20
AflIII A'CryG_T
Cuts at: 1442 2619 3723 4700 1442
Size: 1177 1104 977 2009
Fragments arranged by size:
2009 1177 1104 977
AgeI A'CCGG_T
Cuts at: 3655 3655
Size: 5267
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1226 1682 1972 1226
Size: 456 290 4521
Fragments arranged by size:
4521 456 290
AlwNI CAG_nnn'CTG
Cuts at: 1250 1573 1602 2572 4139 1250
Size: 323 29 970 1567 2378
Fragments arranged by size:
2378 1567 970 323 29
ApaI G_GGCC'C
Cuts at: 277 611 277
Size: 334 4933
ApaLI G'TGCA_C
Cuts at: 4037 4037
Size: 5267
ApoI r'AATT_y
Cuts at: 2271 4620 4876 2271
Size: 2349 256 2662
Fragments arranged by size:
2662 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5267
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5267
AvaI C'yCGr_G
Cuts at: 240 694 2287 240
Size: 454 1593 3220
Fragments arranged by size:
3220 1593 454
BamHI G'GATC_C
Cuts at: 2292 2292
Size: 5267
BanI G'GyrC_C
Cuts at: 244 282 346 373 2283 2697 2945 2980 244
Size: 38 64 27 1910 414 248 35 2531
Fragments arranged by size:
2531 1910 414 248 64 38 35 27
BanII G_rGCy'C
Cuts at: 277 404 611 853 937 2253 2281 3311 277
Size: 127 207 242 84 1316 28 1030 2233
Fragments arranged by size:
2233 1316 1030 242 207 127 84 28
BbeI G_GCGC'C
Cuts at: 248 286 350 377 2701 2949 248
Size: 38 64 27 2324 248 2566
Fragments arranged by size:
2566 2324 248 64 38 27
BciVI GTATCCnnnnn_n'
Cuts at: 2230 3165 3926 5267 2230
Size: 935 761 1341 2230
Fragments arranged by size:
2230 1341 935 761
BclI T'GATC_A
Cuts at: 1143 2787 1143
Size: 1644 3623
BglI GCCn_nnn'nGGC
Cuts at: 1255 1255
Size: 5267
BglII A'GATC_T
Cuts at: 2782 4819 2782
Size: 2037 3230
BlpI GC'TnA_GC
Cuts at: 2379 2379
Size: 5267
Bme1580I G_kGCm'C
Cuts at: 277 611 1271 1482 1620 2892 2985 4041 277
Size: 334 660 211 138 1272 93 1056 1503
Fragments arranged by size:
1503 1272 1056 660 334 211 138 93
BmrI ACTGGGnnnn_n'
Cuts at: 1053 2009 2479 2560 2895 5039 1053
Size: 956 470 81 335 2144 1281
Fragments arranged by size:
2144 1281 956 470 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1296 3634 1296
Size: 2338 2929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1173 1801 1173
Size: 628 4639
Bpu10I CC'TnA_GC
Cuts at: 202 202
Size: 5267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1762 2443 3322 3814 4112 1762
Size: 681 879 492 298 2917
Fragments arranged by size:
2917 879 681 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4971 4971
Size: 5267
BsaAI yAC'GTr
Cuts at: 2620 2620
Size: 5267
BsaBI GATnn'nnATC
Cuts at: 6 716 2781 4818 6
Size: 710 2065 2037 455
Fragments arranged by size:
2065 2037 710 455
BsaHI Gr'CG_yC
Cuts at: 245 254 283 347 374 2698 2946 3648 5144
Size: 9 29 64 27 2324 248 702 1496
Cuts at: 5144 245
Size: 368
Fragments arranged by size:
2324 1496 702 368 248 64 29 27
9
BsaWI w'CCGG_w
Cuts at: 2456 2977 3655 3929 4076 4487 2456
Size: 521 678 274 147 411 3236
Fragments arranged by size:
3236 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 346 376 875 905 1904 1934 346
Size: 30 499 30 999 30 3679
Fragments arranged by size:
3679 999 499 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 297 309 297
Size: 12 5255
BseYI C'CCAG_C
Cuts at: 1240 1640 2692 3450 4027 4523 1240
Size: 400 1052 758 577 496 1984
Fragments arranged by size:
1984 1052 758 577 496 400
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1615 1802 1615
Size: 187 5080
BsiEI CG_ry'CG
Cuts at: 104 364 2809 2855 4063 104
Size: 260 2445 46 1208 1308
Fragments arranged by size:
2445 1308 1208 260 46
BsiHKAI G_wGCw'C
Cuts at: 853 1541 2253 2281 3059 3249 4041 853
Size: 688 712 28 778 190 792 2079
Fragments arranged by size:
2079 792 778 712 688 190 28
BsmI GAATG_Cn'
Cuts at: 657 790 1515 2040 657
Size: 133 725 525 3884
Fragments arranged by size:
3884 725 525 133
Bsp1286I G_dGCh'C
Cuts at: 277 404 611 853 937 1271 1482 1541 1620
Size: 127 207 242 84 334 211 59 79
Cuts at: 1620 2253 2281 2892 2985 3059 3249 3311 4041
Size: 633 28 611 93 74 190 62 730
Cuts at: 4041 277
Size: 1503
Fragments arranged by size:
1503 730 633 611 334 242 211 207
190 127 93 84 79 74 62 59
28
BspEI T'CCGG_A
Cuts at: 4487 4487
Size: 5267
BspHI T'CATG_A
Cuts at: 182 4443 182
Size: 4261 1006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 639 879 1359 2317 2531 2833 3214 639
Size: 240 480 958 214 302 381 2692
Fragments arranged by size:
2692 958 480 381 302 240 214
BsrBI CCG'CTC
Cuts at: 50 195 3559 3613 5097 5262 50
Size: 145 3364 54 1484 165 55
Fragments arranged by size:
3364 1484 165 145 55 54
BsrDI GCAATG_nn'
Cuts at: 3179 3179
Size: 5267
BsrFI r'CCGG_y
Cuts at: 3265 3446 3655 3265
Size: 181 209 4877
Fragments arranged by size:
4877 209 181
BssSI C'ACGA_G
Cuts at: 1270 3538 3896 1270
Size: 2268 358 2641
Fragments arranged by size:
2641 2268 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1573 2644 1573
Size: 1071 4196
BstBI TT'CG_AA
Cuts at: 3628 3628
Size: 5267
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 5267
BstYI r'GATC_y
Cuts at: 1 1599 1737 2210 2292 2759 2782 3117 3363
Size: 1598 138 473 82 467 23 335 246
Cuts at: 3363 4375 4461 4473 4819 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1598 1012 473 467 449 346 335 246
138 86 82 23 12
BtgI C'CryG_G
Cuts at: 138 350 833 879 1259 138
Size: 212 483 46 380 4146
Fragments arranged by size:
4146 483 380 212 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3217 3398 3561 3217
Size: 181 163 4923
Fragments arranged by size:
4923 181 163
BtsI GCAGTG_nn'
Cuts at: 750 871 1300 1574 1721 2057 2510 750
Size: 121 429 274 147 336 453 3507
Fragments arranged by size:
3507 453 429 336 274 147 121
DraI TTT'AAA
Cuts at: 2266 2266
Size: 5267
DraIII CAC_nnn'GTG
Cuts at: 544 544
Size: 5267
DrdI GACnn_nn'nnGTC
Cuts at: 2973 3831 2973
Size: 858 4409
EaeI y'GGCC_r
Cuts at: 513 1944 2852 3026 3417 3444 4789 513
Size: 1431 908 174 391 27 1345 991
Fragments arranged by size:
1431 1345 991 908 391 174 27
EagI C'GGCC_G
Cuts at: 2852 2852
Size: 5267
EarI CTCTTCn'nnn_
Cuts at: 1293 3290 3500 1293
Size: 1997 210 3060
Fragments arranged by size:
3060 1997 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 158 2104 3475 3785 3931 5077 158
Size: 1946 1371 310 146 1146 348
Fragments arranged by size:
1946 1371 1146 348 310 146
EcoICRI GAG'CTC
Cuts at: 851 2251 2279 851
Size: 1400 28 3839
Fragments arranged by size:
3839 1400 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 1173 1212 1678 1801 1865 1885 1991 2245
Size: 431 39 466 123 64 20 106 254
Cuts at: 2245 3092 3524 4271 4539 742
Size: 847 432 747 268 1470
Fragments arranged by size:
1470 847 747 466 432 431 268 254
123 106 64 39 20
EcoO109I rG'GnC_Cy
Cuts at: 274 433 607 2406 274
Size: 159 174 1799 3135
Fragments arranged by size:
3135 1799 174 159
EcoRI G'AATT_C
Cuts at: 2271 2271
Size: 5267
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3286 3286
Size: 5267
FspI TGC'GCA
Cuts at: 3048 3048
Size: 5267
HaeII r_GCGC'y
Cuts at: 248 286 350 377 390 1250 2701 2949 3971
Size: 38 64 27 13 860 1451 248 1022
Cuts at: 3971 4950 248
Size: 979 565
Fragments arranged by size:
1451 1022 979 860 565 248 64 38
27 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 304 336 346 378 604 636 663 695 875
Size: 32 10 32 226 32 27 32 180
Cuts at: 875 907 2195 2227 3109 3139 3141 3171 304
Size: 32 1288 32 882 30 2 30 2400
Fragments arranged by size:
2400 1288 882 226 180 32 32 32
32 32 32 30 30 27 10 2
HincII GTy'rAC
Cuts at: 2096 2306 2096
Size: 210 5057
HindIII A'AGCT_T
Cuts at: 1122 1122
Size: 5267
KasI G'GCGC_C
Cuts at: 244 282 346 373 2697 2945 244
Size: 38 64 27 2324 248 2566
Fragments arranged by size:
2566 2324 248 64 38 27
KpnI G_GTAC'C
Cuts at: 2287 2287
Size: 5267
MluI A'CGCG_T
Cuts at: 4700 4700
Size: 5267
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 326 335 2238 3938 4122 326
Size: 9 1903 1700 184 1471
Fragments arranged by size:
1903 1700 1471 184 9
MscI TGG'CCA
Cuts at: 515 3028 515
Size: 2513 2754
MslI CAynn'nnrTG
Cuts at: 112 722 1664 3256 3383 112
Size: 610 942 1592 127 1996
Fragments arranged by size:
1996 1592 942 610 127
MspA1I CmG'CkG
Cuts at: 140 260 1918 1969 2378 2692 3052 4065 4310
Size: 120 1658 51 409 314 360 1013 245
Cuts at: 4310 140
Size: 1097
Fragments arranged by size:
1658 1097 1013 409 360 314 245 120
51
NaeI GCC'GGC
Cuts at: 3448 3448
Size: 5267
NarI GG'CG_CC
Cuts at: 245 283 347 374 2698 2946 245
Size: 38 64 27 2324 248 2566
Fragments arranged by size:
2566 2324 248 64 38 27
NcoI C'CATG_G
Cuts at: 879 1259 879
Size: 380 4887
NdeI CA'TA_TG
Cuts at: 407 407
Size: 5267
NgoMIV G'CCGG_C
Cuts at: 3446 3446
Size: 5267
NspI r_CATG'y
Cuts at: 994 1524 1663 2320 2820 3727 994
Size: 530 139 657 500 907 2534
Fragments arranged by size:
2534 907 657 530 500 139
PciI A'CATG_T
Cuts at: 3723 3723
Size: 5267
PflMI CCAn_nnn'nTGG
Cuts at: 522 522
Size: 5267
PmeI GTTT'AAAC
Cuts at: 2266 2266
Size: 5267
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1067 1099 4406 4438 1067
Size: 32 3307 32 1896
Fragments arranged by size:
3307 1896 32 32
PspOMI G'GGCC_C
Cuts at: 273 607 273
Size: 334 4933
PstI C_TGCA'G
Cuts at: 2314 2999 2314
Size: 685 4582
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5267
PvuII CAG'CTG
Cuts at: 260 1969 2692 3052 260
Size: 1709 723 360 2475
Fragments arranged by size:
2475 1709 723 360
RsrII CG'GwC_CG
Cuts at: 3462 3462
Size: 5267
SacI G_AGCT'C
Cuts at: 853 2253 2281 853
Size: 1400 28 3839
Fragments arranged by size:
3839 1400 28
SacII CC_GC'GG
Cuts at: 141 141
Size: 5267
SalI G'TCGA_C
Cuts at: 2304 2304
Size: 5267
SapI GCTCTTCn'nnn_
Cuts at: 3290 3500 3290
Size: 210 5057
SbfI CC_TGCA'GG
Cuts at: 2314 2314
Size: 5267
ScaI AGT'ACT
Cuts at: 2148 4784 2148
Size: 2636 2631
SfcI C'TryA_G
Cuts at: 33 2310 2995 3988 4179 33
Size: 2277 685 993 191 1121
Fragments arranged by size:
2277 1121 993 685 191
SfoI GGC'GCC
Cuts at: 246 284 348 375 2699 2947 246
Size: 38 64 27 2324 248 2566
Fragments arranged by size:
2566 2324 248 64 38 27
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5267
SmaI CCC'GGG
Cuts at: 242 696 2289 242
Size: 454 1593 3220
Fragments arranged by size:
3220 1593 454
SmlI C'TyrA_G
Cuts at: 1741 2422 3337 3829 4091 1741
Size: 681 915 492 262 2917
Fragments arranged by size:
2917 915 681 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5267
SphI G_CATG'C
Cuts at: 2320 2820 2320
Size: 500 4767
SspI AAT'ATT
Cuts at: 1164 1401 1164
Size: 237 5030
StyI C'CwwG_G
Cuts at: 879 1259 1467 2401 2755 879
Size: 380 208 934 354 3391
Fragments arranged by size:
3391 934 380 354 208
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 255 1030 2850 2983 3442 3651 255
Size: 775 1820 133 459 209 1871
Fragments arranged by size:
1871 1820 775 459 209 133
TatI w'GTAC_w
Cuts at: 2146 4782 2146
Size: 2636 2631
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1904 1936 4784 4816 1904
Size: 32 2848 32 2355
Fragments arranged by size:
2848 2355 32 32
Tth111I GACn'n_nGTC
Cuts at: 3064 3064
Size: 5267
XbaI T'CTAG_A
Cuts at: 2298 2298
Size: 5267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 412 2762 412
Size: 2350 2917
XmaI C'CCGG_G
Cuts at: 240 694 2287 240
Size: 454 1593 3220
Fragments arranged by size:
3220 1593 454
XmnI GAAnn'nnTTC
Cuts at: 713 1991 713
Size: 1278 3989
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HpaI MfeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI