Restriction map for
pF1KB5707
(Circular) (Six-base) MAPSORT of: pF1KB5707.seq Check: 6864 from: 1 to: 5459
pF1KB5707 5459 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1134 1982 1134
Size: 848 4611
AatII G_ACGT'C
Cuts at: 534 619 534
Size: 85 5374
AccI GT'mk_AC
Cuts at: 619 1486 2497 619
Size: 867 1011 3581
Fragments arranged by size:
3581 1011 867
Acc65I G'GTAC_C
Cuts at: 2475 2475
Size: 5459
AclI AA'CG_TT
Cuts at: 5299 5299
Size: 5459
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 770 1740 2112 2387 3284 3716 4463 4731
Size: 283 970 372 275 897 432 747 268
Cuts at: 4731 487
Size: 1215
Fragments arranged by size:
1215 970 897 747 432 372 283 275
268
AflII C'TTAA_G
Cuts at: 394 394
Size: 5459
AflIII A'CryG_T
Cuts at: 302 2811 3915 4892 302
Size: 2509 1104 977 869
Fragments arranged by size:
2509 1104 977 869
AgeI A'CCGG_T
Cuts at: 3847 3847
Size: 5459
AlwNI CAG_nnn'CTG
Cuts at: 1953 2764 4331 1953
Size: 811 1567 3081
Fragments arranged by size:
3081 1567 811
ApaLI G'TGCA_C
Cuts at: 4229 4229
Size: 5459
ApoI r'AATT_y
Cuts at: 691 982 1703 2463 4812 5068 691
Size: 291 721 760 2349 256 1082
Fragments arranged by size:
2349 1082 760 721 291 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5459
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5459
AvaI C'yCGr_G
Cuts at: 2479 2479
Size: 5459
BamHI G'GATC_C
Cuts at: 2484 2484
Size: 5459
BanI G'GyrC_C
Cuts at: 2475 2889 3137 3172 2475
Size: 414 248 35 4762
Fragments arranged by size:
4762 414 248 35
BanII G_rGCy'C
Cuts at: 930 1057 1481 2009 2473 3503 930
Size: 127 424 528 464 1030 2886
Fragments arranged by size:
2886 1030 528 464 424 127
BbeI G_GCGC'C
Cuts at: 2893 3141 2893
Size: 248 5211
BbsI GAAGACnn'nnnn_
Cuts at: 1400 1400
Size: 5459
BbvCI CC'TCA_GC
Cuts at: 373 649 1448 373
Size: 276 799 4384
Fragments arranged by size:
4384 799 276
BciVI GTATCCnnnnn_n'
Cuts at: 350 3357 4118 5459 350
Size: 3007 761 1341 350
Fragments arranged by size:
3007 1341 761 350
BclI T'GATC_A
Cuts at: 137 2979 137
Size: 2842 2617
BglII A'GATC_T
Cuts at: 1167 2974 5011 1167
Size: 1807 2037 1615
Fragments arranged by size:
2037 1807 1615
BlpI GC'TnA_GC
Cuts at: 435 2571 435
Size: 2136 3323
Bme1580I G_kGCm'C
Cuts at: 3084 3177 4233 3084
Size: 93 1056 4310
Fragments arranged by size:
4310 1056 93
BmgBI CAC'GTC
Cuts at: 303 2313 303
Size: 2010 3449
BmrI ACTGGGnnnn_n'
Cuts at: 2671 2752 3087 5231 2671
Size: 81 335 2144 2899
Fragments arranged by size:
2899 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 232 1463 2006 3826 232
Size: 1231 543 1820 1865
Fragments arranged by size:
1865 1820 1231 543
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 1474 208
Size: 1266 4193
Bpu10I CC'TnA_GC
Cuts at: 373 649 686 1448 373
Size: 276 37 762 4384
Fragments arranged by size:
4384 762 276 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1071 1507 2635 3514 4006 4304 1071
Size: 436 1128 879 492 298 2226
Fragments arranged by size:
2226 1128 879 492 436 298
BsaI GGTCTCn'nnnn_
Cuts at: 899 5163 899
Size: 4264 1195
BsaAI yAC'GTr
Cuts at: 1733 2812 1733
Size: 1079 4380
BsaBI GATnn'nnATC
Cuts at: 6 2973 5010 6
Size: 2967 2037 455
Fragments arranged by size:
2967 2037 455
BsaHI Gr'CG_yC
Cuts at: 531 616 1999 2890 3138 3840 5336 531
Size: 85 1383 891 248 702 1496 654
Fragments arranged by size:
1496 1383 891 702 654 248 85
BsaWI w'CCGG_w
Cuts at: 1563 2648 3169 3847 4121 4268 4679 1563
Size: 1085 521 678 274 147 411 2343
Fragments arranged by size:
2343 1085 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 310 340 1030 1060 1934 1964 310
Size: 30 690 30 874 30 3805
Fragments arranged by size:
3805 874 690 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 331 1047 1372 331
Size: 716 325 4418
Fragments arranged by size:
4418 716 325
BseYI C'CCAG_C
Cuts at: 1273 2884 3642 4219 4715 1273
Size: 1611 758 577 496 2017
Fragments arranged by size:
2017 1611 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1960 1960
Size: 5459
BsiEI CG_ry'CG
Cuts at: 104 3001 3047 4255 104
Size: 2897 46 1208 1308
Fragments arranged by size:
2897 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 634 687 964 1057 2473 3251 3441 4233 634
Size: 53 277 93 1416 778 190 792 1860
Fragments arranged by size:
1860 1416 792 778 277 190 93 53
BsmI GAATG_Cn'
Cuts at: 2431 2431
Size: 5459
BsmBI CGTCTCn'nnnn_
Cuts at: 205 293 2319 205
Size: 88 2026 3345
Fragments arranged by size:
3345 2026 88
Bsp1286I G_dGCh'C
Cuts at: 634 687 930 964 1057 1481 2009 2473 3084
Size: 53 243 34 93 424 528 464 611
Cuts at: 3084 3177 3251 3441 3503 4233 634
Size: 93 74 190 62 730 1860
Fragments arranged by size:
1860 730 611 528 464 424 243 190
93 93 74 62 53 34
BspEI T'CCGG_A
Cuts at: 1563 4679 1563
Size: 3116 2343
BspHI T'CATG_A
Cuts at: 1206 2261 4635 1206
Size: 1055 2374 2030
Fragments arranged by size:
2374 2030 1055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 631 1134 1982 2293 2509 2723 3025 3406 631
Size: 503 848 311 216 214 302 381 2684
Fragments arranged by size:
2684 848 503 381 311 302 216 214
BsrBI CCG'CTC
Cuts at: 50 3751 3805 5289 5454 50
Size: 3701 54 1484 165 55
Fragments arranged by size:
3701 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 506 2159 3371 506
Size: 1653 1212 2594
Fragments arranged by size:
2594 1653 1212
BsrFI r'CCGG_y
Cuts at: 3457 3638 3847 3457
Size: 181 209 5069
Fragments arranged by size:
5069 209 181
BsrGI T'GTAC_A
Cuts at: 503 503
Size: 5459
BssSI C'ACGA_G
Cuts at: 162 3730 4088 162
Size: 3568 358 1533
Fragments arranged by size:
3568 1533 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2251 2836 2251
Size: 585 4874
BstBI TT'CG_AA
Cuts at: 3820 3820
Size: 5459
BstXI CCAn_nnnn'nTGG
Cuts at: 710 2353 710
Size: 1643 3816
BstYI r'GATC_y
Cuts at: 1 810 1167 1895 2484 2951 2974 3309 3555
Size: 809 357 728 589 467 23 335 246
Cuts at: 3555 4567 4653 4665 5011 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 809 728 589 467 449 357 346
335 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1487 1487
Size: 5459
BtgI C'CryG_G
Cuts at: 107 635 872 107
Size: 528 237 4694
Fragments arranged by size:
4694 528 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1244 3409 3590 3753 1244
Size: 2165 181 163 2950
Fragments arranged by size:
2950 2165 181 163
BtsI GCAGTG_nn'
Cuts at: 2702 2702
Size: 5459
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1181 1216 1181
Size: 35 5424
DraI TTT'AAA
Cuts at: 2458 2458
Size: 5459
DrdI GACnn_nn'nnGTC
Cuts at: 3165 4023 3165
Size: 858 4601
EaeI y'GGCC_r
Cuts at: 2038 3044 3218 3609 3636 4981 2038
Size: 1006 174 391 27 1345 2516
Fragments arranged by size:
2516 1345 1006 391 174 27
EagI C'GGCC_G
Cuts at: 3044 3044
Size: 5459
EarI CTCTTCn'nnn_
Cuts at: 883 918 2235 3482 3692 883
Size: 35 1317 1247 210 2650
Fragments arranged by size:
2650 1317 1247 210 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3667 3977 4123 5269 3667
Size: 310 146 1146 3857
Fragments arranged by size:
3857 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1055 2471 1055
Size: 1416 4043
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 487 770 1474 1740 2112 2387 3284 3716
Size: 279 283 704 266 372 275 897 432
Cuts at: 3716 4463 4731 208
Size: 747 268 936
Fragments arranged by size:
936 897 747 704 432 372 283 279
275 268 266
EcoO109I rG'GnC_Cy
Cuts at: 1324 2598 1324
Size: 1274 4185
EcoRI G'AATT_C
Cuts at: 2463 2463
Size: 5459
EcoRV GAT'ATC
Cuts at: 561 561
Size: 5459
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3478 3478
Size: 5459
FspI TGC'GCA
Cuts at: 3240 3240
Size: 5459
HaeII r_GCGC'y
Cuts at: 2893 3141 4163 5142 2893
Size: 248 1022 979 3210
Fragments arranged by size:
3210 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 229 261 310 342 748 780 877 909 1030
Size: 32 49 32 406 32 97 32 121
Cuts at: 1030 1062 2256 2288 3301 3331 3333 3363 229
Size: 32 1194 32 1013 30 2 30 2325
Fragments arranged by size:
2325 1194 1013 406 121 97 49 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1674 2498 1674
Size: 824 4635
HindIII A'AGCT_T
Cuts at: 1535 2201 1535
Size: 666 4793
KasI G'GCGC_C
Cuts at: 2889 3137 2889
Size: 248 5211
KpnI G_GTAC'C
Cuts at: 2479 2479
Size: 5459
MluI A'CGCG_T
Cuts at: 4892 4892
Size: 5459
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 832 4130 4314 832
Size: 3298 184 1977
Fragments arranged by size:
3298 1977 184
MscI TGG'CCA
Cuts at: 3220 3220
Size: 5459
MslI CAynn'nnrTG
Cuts at: 3448 3575 3448
Size: 127 5332
MspA1I CmG'CkG
Cuts at: 324 372 943 1074 1452 1720 2570 2884 3244
Size: 48 571 131 378 268 850 314 360
Cuts at: 3244 4257 4502 324
Size: 1013 245 1281
Fragments arranged by size:
1281 1013 850 571 378 360 314 268
245 131 48
NaeI GCC'GGC
Cuts at: 3640 3640
Size: 5459
NarI GG'CG_CC
Cuts at: 2890 3138 2890
Size: 248 5211
NcoI C'CATG_G
Cuts at: 107 872 107
Size: 765 4694
NgoMIV G'CCGG_C
Cuts at: 3638 3638
Size: 5459
NspI r_CATG'y
Cuts at: 2512 3012 3919 2512
Size: 500 907 4052
Fragments arranged by size:
4052 907 500
PasI CC'CwG_GG
Cuts at: 1246 1246
Size: 5459
PciI A'CATG_T
Cuts at: 3915 3915
Size: 5459
PflMI CCAn_nnn'nTGG
Cuts at: 1300 1300
Size: 5459
PfoI T'CCnGG_A
Cuts at: 1792 1792
Size: 5459
PmeI GTTT'AAAC
Cuts at: 2458 2458
Size: 5459
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4598 4630 4598
Size: 32 5427
PsiI TTA'TAA
Cuts at: 2147 2147
Size: 5459
PstI C_TGCA'G
Cuts at: 1949 2117 2506 3191 1949
Size: 168 389 685 4217
Fragments arranged by size:
4217 685 389 168
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5459
PvuII CAG'CTG
Cuts at: 324 372 1452 1720 2884 3244 324
Size: 48 1080 268 1164 360 2539
Fragments arranged by size:
2539 1164 1080 360 268 48
RsrII CG'GwC_CG
Cuts at: 3654 3654
Size: 5459
SacI G_AGCT'C
Cuts at: 1057 2473 1057
Size: 1416 4043
SalI G'TCGA_C
Cuts at: 2496 2496
Size: 5459
SapI GCTCTTCn'nnn_
Cuts at: 918 2235 3482 3692 918
Size: 1317 1247 210 2685
Fragments arranged by size:
2685 1317 1247 210
SbfI CC_TGCA'GG
Cuts at: 2506 2506
Size: 5459
ScaI AGT'ACT
Cuts at: 4976 4976
Size: 5459
SexAI A'CCwGG_T
Cuts at: 622 622
Size: 5459
SfcI C'TryA_G
Cuts at: 33 1945 2113 2502 3187 4180 4371 33
Size: 1912 168 389 685 993 191 1121
Fragments arranged by size:
1912 1121 993 685 389 191 168
SfoI GGC'GCC
Cuts at: 2891 3139 2891
Size: 248 5211
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5459
SmaI CCC'GGG
Cuts at: 2481 2481
Size: 5459
SmlI C'TyrA_G
Cuts at: 394 1050 1522 2614 3529 4021 4283 394
Size: 656 472 1092 915 492 262 1570
Fragments arranged by size:
1570 1092 915 656 492 472 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5459
SphI G_CATG'C
Cuts at: 2512 3012 2512
Size: 500 4959
SspI AAT'ATT
Cuts at: 2398 2398
Size: 5459
StyI C'CwwG_G
Cuts at: 107 872 2593 2947 107
Size: 765 1721 354 2619
Fragments arranged by size:
2619 1721 765 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1784 1953 3042 3175 3634 3843 1784
Size: 169 1089 133 459 209 3400
Fragments arranged by size:
3400 1089 459 209 169 133
TatI w'GTAC_w
Cuts at: 503 668 2413 4974 503
Size: 165 1745 2561 988
Fragments arranged by size:
2561 1745 988 165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4976 5008 4976
Size: 32 5427
Tth111I GACn'n_nGTC
Cuts at: 3256 3256
Size: 5459
XbaI T'CTAG_A
Cuts at: 2490 2490
Size: 5459
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2954 2954
Size: 5459
XmaI C'CCGG_G
Cuts at: 2479 2479
Size: 5459
ZraI GAC'GTC
Cuts at: 532 617 532
Size: 85 5374
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AhdI AleI AlfI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmtI BsiWI BssHII BstEII
Bsu36I ClaI DraIII EcoNI FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI