Restriction map for
pF1KB5675
(Circular) (Six-base) MAPSORT of: pF1KB5675.seq Check: 1011 from: 1 to: 6146
pF1KB5675 6146 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 314 807 1326 2819 314
Size: 493 519 1493 3641
Fragments arranged by size:
3641 1493 519 493
AccI GT'mk_AC
Cuts at: 3184 3184
Size: 6146
Acc65I G'GTAC_C
Cuts at: 3162 3162
Size: 6146
AclI AA'CG_TT
Cuts at: 1951 5986 1951
Size: 4035 2111
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 1815 2076 2581 3971 4403 4756 5418 862
Size: 953 261 505 1390 432 353 662 1590
Fragments arranged by size:
1590 1390 953 662 505 432 353 261
AflIII A'CryG_T
Cuts at: 1085 3498 5298 5579 1085
Size: 2413 1800 281 1652
Fragments arranged by size:
2413 1800 1652 281
AgeI A'CCGG_T
Cuts at: 1273 1273
Size: 6146
AleI CACnn'nnGTG
Cuts at: 392 602 871 2974 392
Size: 210 269 2103 3564
Fragments arranged by size:
3564 2103 269 210
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 521 1756 2809 2885 521
Size: 1235 1053 76 3782
Fragments arranged by size:
3782 1235 1053 76
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1786 1818 1786
Size: 32 6114
AlwNI CAG_nnn'CTG
Cuts at: 804 939 1607 3451 4889 804
Size: 135 668 1844 1438 2061
Fragments arranged by size:
2061 1844 1438 668 135
ApaI G_GGCC'C
Cuts at: 2836 2836
Size: 6146
ApaLI G'TGCA_C
Cuts at: 301 1406 2173 2545 2806 4984 301
Size: 1105 767 372 261 2178 1463
Fragments arranged by size:
2178 1463 1105 767 372 261
ApoI r'AATT_y
Cuts at: 3150 5499 5755 3150
Size: 2349 256 3541
Fragments arranged by size:
3541 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6146
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6146
AvaI C'yCGr_G
Cuts at: 248 710 961 2724 2749 3166 248
Size: 462 251 1763 25 417 3228
Fragments arranged by size:
3228 1763 462 417 251 25
BamHI G'GATC_C
Cuts at: 3171 3171
Size: 6146
BanI G'GyrC_C
Cuts at: 112 491 545 604 764 1459 1854 2080 2231
Size: 379 54 59 160 695 395 226 151
Cuts at: 2231 2627 2734 3162 3576 3824 3859 112
Size: 396 107 428 414 248 35 2399
Fragments arranged by size:
2399 695 428 414 396 395 379 248
226 160 151 107 59 54 35
BanII G_rGCy'C
Cuts at: 1060 2136 2417 2540 2757 2836 3160 4190 1060
Size: 1076 281 123 217 79 324 1030 3016
Fragments arranged by size:
3016 1076 1030 324 281 217 123 79
BbeI G_GCGC'C
Cuts at: 116 549 768 2631 2738 3580 3828 116
Size: 433 219 1863 107 842 248 2434
Fragments arranged by size:
2434 1863 842 433 248 219 107
BbsI GAAGACnn'nnnn_
Cuts at: 2158 2158
Size: 6146
BbvCI CC'TCA_GC
Cuts at: 1318 1318
Size: 6146
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1413 1447 1413
Size: 34 6112
BciVI GTATCCnnnnn_n'
Cuts at: 253 4044 5100 6146 253
Size: 3791 1056 1046 253
Fragments arranged by size:
3791 1056 1046 253
BclI T'GATC_A
Cuts at: 3666 3666
Size: 6146
BglI GCCn_nnn'nGGC
Cuts at: 433 433
Size: 6146
BglII A'GATC_T
Cuts at: 3661 5698 3661
Size: 2037 4109
BlpI GC'TnA_GC
Cuts at: 1615 3258 1615
Size: 1643 4503
Bme1580I G_kGCm'C
Cuts at: 305 1410 1745 2177 2549 2810 2836 3771 3864
Size: 1105 335 432 372 261 26 935 93
Cuts at: 3864 4988 305
Size: 1124 1463
Fragments arranged by size:
1463 1124 1105 935 432 372 335 261
93 26
BmgBI CAC'GTC
Cuts at: 330 330
Size: 6146
BmrI ACTGGGnnnn_n'
Cuts at: 3358 3439 3774 5918 3358
Size: 81 335 2144 3586
Fragments arranged by size:
3586 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 738 3018 4513 738
Size: 2280 1495 2371
Fragments arranged by size:
2371 2280 1495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 664 826 1372 1420 2257 2338 2386 2554
Size: 93 162 546 48 837 81 48 168
Cuts at: 2554 2988 571
Size: 434 3729
Fragments arranged by size:
3729 837 546 434 168 162 93 81
48 48
Bpu10I CC'TnA_GC
Cuts at: 1318 2899 1318
Size: 1581 4565
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2943 2995 3322 4201 4915 5213 2943
Size: 52 327 879 714 298 3876
Fragments arranged by size:
3876 879 714 327 298 52
BsaI GGTCTCn'nnnn_
Cuts at: 521 2959 5850 521
Size: 2438 2891 817
Fragments arranged by size:
2891 2438 817
BsaAI yAC'GTr
Cuts at: 462 1476 3499 462
Size: 1014 2023 3109
Fragments arranged by size:
3109 2023 1014
BsaBI GATnn'nnATC
Cuts at: 6 2455 3660 5697 6
Size: 2449 1205 2037 455
Fragments arranged by size:
2449 2037 1205 455
BsaHI Gr'CG_yC
Cuts at: 113 546 765 1448 2628 2735 3577 3825 4527
Size: 433 219 683 1180 107 842 248 702
Cuts at: 4527 6023 113
Size: 1496 236
Fragments arranged by size:
1496 1180 842 702 683 433 248 236
219 107
BsaWI w'CCGG_w
Cuts at: 1273 1396 1791 2887 3335 3856 4534 4945 5092
Size: 123 395 1096 448 521 678 411 147
Cuts at: 5092 5366 1273
Size: 274 2053
Fragments arranged by size:
2053 1096 678 521 448 411 395 274
147 123
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 805 835 1786 1816 2230 2260 2590 2620 2663
Size: 30 951 30 414 30 330 30 43
Cuts at: 2663 2693 2851 2881 2920 2950 805
Size: 30 158 30 39 30 4001
Fragments arranged by size:
4001 951 414 330 158 43 39 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2385 2747 2385
Size: 362 5784
BseYI C'CCAG_C
Cuts at: 569 911 1511 1744 1765 2129 2828 3571 4329
Size: 342 600 233 21 364 699 743 758
Cuts at: 4329 4994 5402 569
Size: 665 408 1313
Fragments arranged by size:
1313 758 743 699 665 600 408 364
342 233 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 418 2185 301
Size: 117 1767 4262
Fragments arranged by size:
4262 1767 117
BsiEI CG_ry'CG
Cuts at: 104 854 1294 3688 3734 4964 104
Size: 750 440 2394 46 1230 1286
Fragments arranged by size:
2394 1286 1230 750 440 46
BsiHKAI G_wGCw'C
Cuts at: 249 305 1410 1436 1940 2177 2540 2549 2810
Size: 56 1105 26 504 237 363 9 261
Cuts at: 2810 2864 3160 3938 4128 4988 249
Size: 54 296 778 190 860 1407
Fragments arranged by size:
1407 1105 860 778 504 363 296 261
237 190 56 54 26 9
BsmI GAATG_Cn'
Cuts at: 1083 1083
Size: 6146
Bsp1286I G_dGCh'C
Cuts at: 249 305 1060 1410 1436 1745 1940 2136 2177
Size: 56 755 350 26 309 195 196 41
Cuts at: 2177 2417 2540 2549 2757 2810 2836 2864 3160
Size: 240 123 9 208 53 26 28 296
Cuts at: 3160 3771 3864 3938 4128 4190 4988 249
Size: 611 93 74 190 62 798 1407
Fragments arranged by size:
1407 798 755 611 350 309 296 240
208 196 195 190 123 93 74 62
56 53 41 28 26 26 9
BspEI T'CCGG_A
Cuts at: 1396 1791 2887 1396
Size: 395 1096 4655
Fragments arranged by size:
4655 1096 395
BspHI T'CATG_A
Cuts at: 4578 4578
Size: 6146
BspMI ACCTGCnnnn'nnnn_
Cuts at: 314 807 1326 2801 2819 3196 3410 3712 4093
Size: 493 519 1475 18 377 214 302 381
Cuts at: 4093 314
Size: 2367
Fragments arranged by size:
2367 1475 519 493 381 377 302 214
18
BsrBI CCG'CTC
Cuts at: 50 201 1614 2498 4438 4492 5976 6141 50
Size: 151 1413 884 1940 54 1484 165 55
Fragments arranged by size:
1940 1484 1413 884 165 151 55 54
BsrDI GCAATG_nn'
Cuts at: 666 2355 4058 666
Size: 1689 1703 2754
Fragments arranged by size:
2754 1703 1689
BsrFI r'CCGG_y
Cuts at: 241 391 1019 1273 1293 1692 2675 4144 4325
Size: 150 628 254 20 399 983 1469 181
Cuts at: 4325 241
Size: 2062
Fragments arranged by size:
2062 1469 983 628 399 254 181 150
20
BssHII G'CGCG_C
Cuts at: 1492 1630 1492
Size: 138 6008
BssSI C'ACGA_G
Cuts at: 4417 5125 4417
Size: 708 5438
BstAPI GCAn_nnn'nTGC
Cuts at: 309 2007 2172 3523 309
Size: 1698 165 1351 2932
Fragments arranged by size:
2932 1698 1351 165
BstBI TT'CG_AA
Cuts at: 4507 4507
Size: 6146
BstXI CCAn_nnnn'nTGG
Cuts at: 981 1752 2976 981
Size: 771 1224 4151
Fragments arranged by size:
4151 1224 771
BstYI r'GATC_y
Cuts at: 1 438 1903 2138 2360 3171 3638 3661 3996
Size: 437 1465 235 222 811 467 23 335
Cuts at: 3996 4242 4548 4560 4646 5698 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1465 1052 811 467 449 437 335 306
246 235 222 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2076 2708 2076
Size: 632 5514
BtgI C'CryG_G
Cuts at: 107 347 854 1052 2417 3089 107
Size: 240 507 198 1365 672 3164
Fragments arranged by size:
3164 1365 672 507 240 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1438 1763 4096 4277 4440 1438
Size: 325 2333 181 163 3144
Fragments arranged by size:
3144 2333 325 181 163
BtsI GCAGTG_nn'
Cuts at: 1007 1155 1813 3389 1007
Size: 148 658 1576 3764
Fragments arranged by size:
3764 1576 658 148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 766 801 766
Size: 35 6111
DraI TTT'AAA
Cuts at: 3145 3145
Size: 6146
DraIII CAC_nnn'GTG
Cuts at: 1044 2148 2637 1044
Size: 1104 489 4553
Fragments arranged by size:
4553 1104 489
DrdI GACnn_nn'nnGTC
Cuts at: 3852 5196 3852
Size: 1344 4802
EaeI y'GGCC_r
Cuts at: 193 702 1004 1291 1373 1478 2352 2822 2978
Size: 509 302 287 82 105 874 470 156
Cuts at: 2978 3068 3731 3905 4296 4323 5668 193
Size: 90 663 174 391 27 1345 671
Fragments arranged by size:
1345 874 671 663 509 470 391 302
287 174 156 105 90 82 27
EagI C'GGCC_G
Cuts at: 1291 3731 1291
Size: 2440 3706
EarI CTCTTCn'nnn_
Cuts at: 868 4169 4379 868
Size: 3301 210 2635
Fragments arranged by size:
3301 2635 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1680 2701 4354 5096 5242 5956 1680
Size: 1021 1653 742 146 714 1870
Fragments arranged by size:
1870 1653 1021 742 714 146
EcoICRI GAG'CTC
Cuts at: 2538 3158 2538
Size: 620 5526
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 664 826 862 1372 1420 1815 2076 2257
Size: 93 162 36 510 48 395 261 181
Cuts at: 2257 2338 2386 2554 2581 2988 3971 4403 4756
Size: 81 48 168 27 407 983 432 353
Cuts at: 4756 5418 571
Size: 662 1299
Fragments arranged by size:
1299 983 662 510 432 407 395 353
261 181 168 162 93 81 48 48
36 27
EcoNI CCTnn'n_nnAGG
Cuts at: 1417 1964 1417
Size: 547 5599
EcoO109I rG'GnC_Cy
Cuts at: 166 948 1384 1516 1722 2697 2790 2832 2833
Size: 782 436 132 206 975 93 42 1
Cuts at: 2833 2928 3031 3285 166
Size: 95 103 254 3027
Fragments arranged by size:
3027 975 782 436 254 206 132 103
95 93 42 1
EcoRI G'AATT_C
Cuts at: 3150 3150
Size: 6146
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2175 2349 4165 2175
Size: 174 1816 4156
Fragments arranged by size:
4156 1816 174
FseI GG_CCGG'CC
Cuts at: 1297 1297
Size: 6146
FspI TGC'GCA
Cuts at: 734 1130 2785 3927 734
Size: 396 1655 1142 2953
Fragments arranged by size:
2953 1655 1142 396
HaeII r_GCGC'y
Cuts at: 116 549 768 1317 1360 2631 2738 2906 3580
Size: 433 219 549 43 1271 107 168 674
Cuts at: 3580 3828 5058 5829 116
Size: 248 1230 771 433
Fragments arranged by size:
1271 1230 771 674 549 433 433 248
219 168 107 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2059 2091 2590 2622 2663 2695 2920 2952 3988
Size: 32 499 32 41 32 225 32 1036
Cuts at: 3988 4018 4020 4050 2059
Size: 30 2 30 4155
Fragments arranged by size:
4155 1036 499 225 41 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2442 3185 2442
Size: 743 5403
KasI G'GCGC_C
Cuts at: 112 545 764 2627 2734 3576 3824 112
Size: 433 219 1863 107 842 248 2434
Fragments arranged by size:
2434 1863 842 433 248 219 107
KpnI G_GTAC'C
Cuts at: 3166 3166
Size: 6146
MluI A'CGCG_T
Cuts at: 5579 5579
Size: 6146
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4905 5089 4905
Size: 184 5962
MscI TGG'CCA
Cuts at: 1006 1480 2824 2980 3907 1006
Size: 474 1344 156 927 3245
Fragments arranged by size:
3245 1344 927 474 156
MslI CAynn'nnrTG
Cuts at: 320 392 460 602 871 1045 2974 4135 4262
Size: 72 68 142 269 174 1929 1161 127
Cuts at: 4262 320
Size: 2204
Fragments arranged by size:
2204 1929 1161 269 174 142 127 72
68
MspA1I CmG'CkG
Cuts at: 856 939 1119 1350 1457 2115 2129 2229 2316
Size: 83 180 231 107 658 14 100 87
Cuts at: 2316 2478 2828 3060 3257 3571 3931 4715 4960
Size: 162 350 232 197 314 360 784 245
Cuts at: 4960 856
Size: 2042
Fragments arranged by size:
2042 784 658 360 350 314 245 232
231 197 180 162 107 100 87 83
14
NaeI GCC'GGC
Cuts at: 1295 4327 1295
Size: 3032 3114
NarI GG'CG_CC
Cuts at: 113 546 765 2628 2735 3577 3825 113
Size: 433 219 1863 107 842 248 2434
Fragments arranged by size:
2434 1863 842 433 248 219 107
NcoI C'CATG_G
Cuts at: 107 1052 3089 107
Size: 945 2037 3164
Fragments arranged by size:
3164 2037 945
NgoMIV G'CCGG_C
Cuts at: 1293 4325 1293
Size: 3032 3114
NspI r_CATG'y
Cuts at: 272 294 1089 2475 3199 3699 5302 272
Size: 22 795 1386 724 500 1603 1116
Fragments arranged by size:
1603 1386 1116 795 724 500 22
PasI CC'CwG_GG
Cuts at: 1014 1659 1824 2502 1014
Size: 645 165 678 4658
Fragments arranged by size:
4658 678 645 165
PciI A'CATG_T
Cuts at: 1085 5298 1085
Size: 4213 1933
PflMI CCAn_nnn'nTGG
Cuts at: 1088 1476 1088
Size: 388 5758
PfoI T'CCnGG_A
Cuts at: 407 2363 407
Size: 1956 4190
PmeI GTTT'AAAC
Cuts at: 3145 3145
Size: 6146
PmlI CAC'GTG
Cuts at: 1476 1476
Size: 6146
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1786 1818 4592 4624 1786
Size: 32 2774 32 3308
Fragments arranged by size:
3308 2774 32 32
PpuMI rG'GwC_Cy
Cuts at: 948 1384 1722 2697 2790 2928 948
Size: 436 338 975 93 138 4166
Fragments arranged by size:
4166 975 436 338 138 93
PsiI TTA'TAA
Cuts at: 1652 1652
Size: 6146
PspOMI G'GGCC_C
Cuts at: 2832 2832
Size: 6146
PstI C_TGCA'G
Cuts at: 1337 2664 2963 3193 3878 1337
Size: 1327 299 230 685 3605
Fragments arranged by size:
3605 1327 685 299 230
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6146
PvuII CAG'CTG
Cuts at: 1119 1350 2478 2828 3060 3571 3931 1119
Size: 231 1128 350 232 511 360 3334
Fragments arranged by size:
3334 1128 511 360 350 232 231
RsrII CG'GwC_CG
Cuts at: 4341 4341
Size: 6146
SacI G_AGCT'C
Cuts at: 2540 3160 2540
Size: 620 5526
SacII CC_GC'GG
Cuts at: 857 857
Size: 6146
SalI G'TCGA_C
Cuts at: 3183 3183
Size: 6146
SanDI GG'GwC_CC
Cuts at: 1384 1384
Size: 6146
SapI GCTCTTCn'nnn_
Cuts at: 4169 4379 4169
Size: 210 5936
SbfI CC_TGCA'GG
Cuts at: 1337 2963 3193 1337
Size: 1626 230 4290
Fragments arranged by size:
4290 1626 230
ScaI AGT'ACT
Cuts at: 358 5663 358
Size: 5305 841
SexAI A'CCwGG_T
Cuts at: 1213 2144 2633 1213
Size: 931 489 4726
Fragments arranged by size:
4726 931 489
SfcI C'TryA_G
Cuts at: 33 1333 2660 2959 3189 3874 4842 5033 33
Size: 1300 1327 299 230 685 968 191 1146
Fragments arranged by size:
1327 1300 1146 968 685 299 230 191
SfoI GGC'GCC
Cuts at: 114 547 766 2629 2736 3578 3826 114
Size: 433 219 1863 107 842 248 2434
Fragments arranged by size:
2434 1863 842 433 248 219 107
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6146
SgrAI Cr'CCGG_yG
Cuts at: 391 391
Size: 6146
SmaI CCC'GGG
Cuts at: 2726 3168 2726
Size: 442 5704
SmlI C'TyrA_G
Cuts at: 2922 3010 3301 4216 4930 5192 2922
Size: 88 291 915 714 262 3876
Fragments arranged by size:
3876 915 714 291 262 88
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6146
SphI G_CATG'C
Cuts at: 3199 3699 3199
Size: 500 5646
SspI AAT'ATT
Cuts at: 1760 1760
Size: 6146
StuI AGG'CCT
Cuts at: 721 2074 721
Size: 1353 4793
StyI C'CwwG_G
Cuts at: 107 898 974 1052 2159 3089 3280 3634 107
Size: 791 76 78 1107 930 191 354 2619
Fragments arranged by size:
2619 1107 930 791 354 191 78 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 405 561 610 2607 3729 3862 4321 4530 405
Size: 156 49 1997 1122 133 459 209 2021
Fragments arranged by size:
2021 1997 1122 459 209 156 133 49
TatI w'GTAC_w
Cuts at: 356 401 965 5661 356
Size: 45 564 4696 841
Fragments arranged by size:
4696 841 564 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 805 837 907 939 1877 1909 2230 2262 5663
Size: 32 70 32 938 32 321 32 3401
Cuts at: 5663 5695 805
Size: 32 1256
Fragments arranged by size:
3401 1256 938 321 70 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 177 415 1450 3943 177
Size: 238 1035 2493 2380
Fragments arranged by size:
2493 2380 1035 238
XbaI T'CTAG_A
Cuts at: 3177 3177
Size: 6146
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 361 625 3078 3641 361
Size: 264 2453 563 2866
Fragments arranged by size:
2866 2453 563 264
XmaI C'CCGG_G
Cuts at: 2724 3166 2724
Size: 442 5704
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmBI BsrGI BstEII BstZ17I ClaI EcoRV
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PshAI PspXI PsrI SfiI SnaBI SrfI
SwaI XhoI XmnI ZraI