Restriction map for
pF1KB5674
(Circular) (Six-base) MAPSORT of: pF1KB5674.seq Check: 1916 from: 1 to: 4418
pF1KB5674 4418 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AatII G_ACGT'C
Cuts at: 254 254
Size: 4418
AccI GT'mk_AC
Cuts at: 1456 1456
Size: 4418
Acc65I G'GTAC_C
Cuts at: 1434 1434
Size: 4418
AclI AA'CG_TT
Cuts at: 4258 4258
Size: 4418
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 1232 2243 2675 3028 3690 566
Size: 666 1011 432 353 662 1294
Fragments arranged by size:
1294 1011 666 662 432 353
AflIII A'CryG_T
Cuts at: 1343 1770 3570 3851 1343
Size: 427 1800 281 1910
Fragments arranged by size:
1910 1800 427 281
AleI CACnn'nnGTG
Cuts at: 752 752
Size: 4418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 770 770
Size: 4418
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 109 141 109
Size: 32 4386
AlwNI CAG_nnn'CTG
Cuts at: 1723 3161 1723
Size: 1438 2980
ApaI G_GGCC'C
Cuts at: 367 737 367
Size: 370 4048
ApaLI G'TGCA_C
Cuts at: 745 3256 745
Size: 2511 1907
ApoI r'AATT_y
Cuts at: 522 943 1422 3771 4027 522
Size: 421 479 2349 256 913
Fragments arranged by size:
2349 913 479 421 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4418
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4418
AvaI C'yCGr_G
Cuts at: 611 1015 1291 1438 611
Size: 404 276 147 3591
Fragments arranged by size:
3591 404 276 147
BamHI G'GATC_C
Cuts at: 1443 1443
Size: 4418
BanI G'GyrC_C
Cuts at: 937 1249 1434 1848 2096 2131 937
Size: 312 185 414 248 35 3224
Fragments arranged by size:
3224 414 312 248 185 35
BanII G_rGCy'C
Cuts at: 367 737 1010 1432 2462 367
Size: 370 273 422 1030 2323
Fragments arranged by size:
2323 1030 422 370 273
BbeI G_GCGC'C
Cuts at: 1852 2100 1852
Size: 248 4170
BbsI GAAGACnn'nnnn_
Cuts at: 521 1234 521
Size: 713 3705
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 408 442 1291 1325 408
Size: 34 849 34 3501
Fragments arranged by size:
3501 849 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2316 3372 4418 2316
Size: 1056 1046 2316
Fragments arranged by size:
2316 1056 1046
BclI T'GATC_A
Cuts at: 1938 1938
Size: 4418
BglII A'GATC_T
Cuts at: 617 1933 3970 617
Size: 1316 2037 1065
Fragments arranged by size:
2037 1316 1065
BlpI GC'TnA_GC
Cuts at: 1530 1530
Size: 4418
Bme1580I G_kGCm'C
Cuts at: 367 737 749 1254 2043 2136 3260 367
Size: 370 12 505 789 93 1124 1525
Fragments arranged by size:
1525 1124 789 505 370 93 12
BmrI ACTGGGnnnn_n'
Cuts at: 1630 1711 2046 4190 1630
Size: 81 335 2144 1858
Fragments arranged by size:
2144 1858 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2785 2785
Size: 4418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1078 1024
Size: 54 4364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1594 2473 3187 3485 1594
Size: 879 714 298 2527
Fragments arranged by size:
2527 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1111 4122 1111
Size: 3011 1407
BsaAI yAC'GTr
Cuts at: 1771 1771
Size: 4418
BsaBI GATnn'nnATC
Cuts at: 6 1932 3969 6
Size: 1926 2037 455
Fragments arranged by size:
2037 1926 455
BsaHI Gr'CG_yC
Cuts at: 251 1849 2097 2799 4295 251
Size: 1598 248 702 1496 374
Fragments arranged by size:
1598 1496 702 374 248
BsaWI w'CCGG_w
Cuts at: 1607 2128 2806 3217 3364 3638 1607
Size: 521 678 411 147 274 2387
Fragments arranged by size:
2387 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 388 418 388
Size: 30 4388
BseYI C'CCAG_C
Cuts at: 152 1266 1843 2601 3266 3674 152
Size: 1114 577 758 665 408 896
Fragments arranged by size:
1114 896 758 665 577 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 776 988 776
Size: 212 4206
BsiEI CG_ry'CG
Cuts at: 104 1370 1960 2006 3236 104
Size: 1266 590 46 1230 1286
Fragments arranged by size:
1286 1266 1230 590 46
BsiHKAI G_wGCw'C
Cuts at: 731 749 1432 2210 2400 3260 731
Size: 18 683 778 190 860 1889
Fragments arranged by size:
1889 860 778 683 190 18
BsmBI CGTCTCn'nnnn_
Cuts at: 242 242
Size: 4418
Bsp1286I G_dGCh'C
Cuts at: 367 731 737 749 1010 1254 1432 2043 2136
Size: 364 6 12 261 244 178 611 93
Cuts at: 2136 2210 2400 2462 3260 367
Size: 74 190 62 798 1525
Fragments arranged by size:
1525 798 611 364 261 244 190 178
93 74 62 12 6
BspHI T'CATG_A
Cuts at: 2850 2850
Size: 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 758 805 1468 1682 1984 2365 758
Size: 47 663 214 302 381 2811
Fragments arranged by size:
2811 663 381 302 214 47
BsrBI CCG'CTC
Cuts at: 50 2710 2764 4248 4413 50
Size: 2660 54 1484 165 55
Fragments arranged by size:
2660 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2330 2330
Size: 4418
BsrFI r'CCGG_y
Cuts at: 1369 2416 2597 1369
Size: 1047 181 3190
Fragments arranged by size:
3190 1047 181
BssSI C'ACGA_G
Cuts at: 994 2689 3397 994
Size: 1695 708 2015
Fragments arranged by size:
2015 1695 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1795 1795
Size: 4418
BstBI TT'CG_AA
Cuts at: 2779 2779
Size: 4418
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4418
BstYI r'GATC_y
Cuts at: 1 617 1443 1910 1933 2268 2514 2820 2832
Size: 616 826 467 23 335 246 306 12
Cuts at: 2832 2918 3970 1
Size: 86 1052 449
Fragments arranged by size:
1052 826 616 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4418
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2368 2549 2712 2368
Size: 181 163 4074
Fragments arranged by size:
4074 181 163
BtsI GCAGTG_nn'
Cuts at: 747 759 1285 1661 747
Size: 12 526 376 3504
Fragments arranged by size:
3504 526 376 12
DraI TTT'AAA
Cuts at: 1417 1417
Size: 4418
DrdI GACnn_nn'nnGTC
Cuts at: 655 2124 3468 655
Size: 1469 1344 1605
Fragments arranged by size:
1605 1469 1344
EaeI y'GGCC_r
Cuts at: 158 209 481 1039 1367 2003 2177 2568 2595
Size: 51 272 558 328 636 174 391 27
Cuts at: 2595 3940 158
Size: 1345 636
Fragments arranged by size:
1345 636 636 558 391 328 272 174
51 27
EagI C'GGCC_G
Cuts at: 1367 2003 1367
Size: 636 3782
EarI CTCTTCn'nnn_
Cuts at: 966 1109 2441 2651 966
Size: 143 1332 210 2733
Fragments arranged by size:
2733 1332 210 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2626 3368 3514 4228 2626
Size: 742 146 714 2816
Fragments arranged by size:
2816 742 714 146
EcoICRI GAG'CTC
Cuts at: 1430 1430
Size: 4418
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 1024 1078 1232 2243 2675 3028 3690 566
Size: 458 54 154 1011 432 353 662 1294
Fragments arranged by size:
1294 1011 662 458 432 353 154 54
EcoO109I rG'GnC_Cy
Cuts at: 363 364 999 1557 363
Size: 1 635 558 3224
Fragments arranged by size:
3224 635 558 1
EcoRI G'AATT_C
Cuts at: 1422 1422
Size: 4418
EcoRV GAT'ATC
Cuts at: 174 552 843 174
Size: 378 291 3749
Fragments arranged by size:
3749 378 291
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2437 2437
Size: 4418
FspI TGC'GCA
Cuts at: 471 2199 471
Size: 1728 2690
FspAI rTGC'GCAy
Cuts at: 471 471
Size: 4418
HaeII r_GCGC'y
Cuts at: 1376 1852 2100 3330 4101 1376
Size: 476 248 1230 771 1693
Fragments arranged by size:
1693 1230 771 476 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 541 573 697 729 2260 2290 2292 2322 541
Size: 32 124 32 1531 30 2 30 2637
Fragments arranged by size:
2637 1531 124 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1341 1457 1341
Size: 116 4302
HindIII A'AGCT_T
Cuts at: 214 214
Size: 4418
KasI G'GCGC_C
Cuts at: 1848 2096 1848
Size: 248 4170
KpnI G_GTAC'C
Cuts at: 1438 1438
Size: 4418
MluI A'CGCG_T
Cuts at: 3851 3851
Size: 4418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 201 253 3177 3361 201
Size: 52 2924 184 1258
Fragments arranged by size:
2924 1258 184 52
MscI TGG'CCA
Cuts at: 211 483 2179 211
Size: 272 1696 2450
Fragments arranged by size:
2450 1696 272
MslI CAynn'nnrTG
Cuts at: 112 752 1348 2407 2534 112
Size: 640 596 1059 127 1996
Fragments arranged by size:
1996 1059 640 596 127
MspA1I CmG'CkG
Cuts at: 906 1529 1843 2203 2987 3232 906
Size: 623 314 360 784 245 2092
Fragments arranged by size:
2092 784 623 360 314 245
NaeI GCC'GGC
Cuts at: 1371 2599 1371
Size: 1228 3190
NarI GG'CG_CC
Cuts at: 1849 2097 1849
Size: 248 4170
NcoI C'CATG_G
Cuts at: 107 107
Size: 4418
NgoMIV G'CCGG_C
Cuts at: 1369 2597 1369
Size: 1228 3190
NspI r_CATG'y
Cuts at: 383 1347 1471 1971 3574 383
Size: 964 124 500 1603 1227
Fragments arranged by size:
1603 1227 964 500 124
PasI CC'CwG_GG
Cuts at: 360 360
Size: 4418
PciI A'CATG_T
Cuts at: 1343 3570 1343
Size: 2227 2191
PflMI CCAn_nnn'nTGG
Cuts at: 1069 1069
Size: 4418
PfoI T'CCnGG_A
Cuts at: 145 1055 145
Size: 910 3508
PmeI GTTT'AAAC
Cuts at: 1417 1417
Size: 4418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2864 2896 2864
Size: 32 4386
PpuMI rG'GwC_Cy
Cuts at: 999 999
Size: 4418
PshAI GACnn'nnGTC
Cuts at: 254 269 254
Size: 15 4403
PspOMI G'GGCC_C
Cuts at: 363 733 363
Size: 370 4048
PstI C_TGCA'G
Cuts at: 754 1465 2150 754
Size: 711 685 3022
Fragments arranged by size:
3022 711 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4418
PvuII CAG'CTG
Cuts at: 906 1843 2203 906
Size: 937 360 3121
Fragments arranged by size:
3121 937 360
RsrII CG'GwC_CG
Cuts at: 2613 2613
Size: 4418
SacI G_AGCT'C
Cuts at: 1432 1432
Size: 4418
SalI G'TCGA_C
Cuts at: 1455 1455
Size: 4418
SapI GCTCTTCn'nnn_
Cuts at: 2441 2651 2441
Size: 210 4208
SbfI CC_TGCA'GG
Cuts at: 1465 1465
Size: 4418
ScaI AGT'ACT
Cuts at: 3935 3935
Size: 4418
SfcI C'TryA_G
Cuts at: 33 750 1386 1461 2146 3114 3305 33
Size: 717 636 75 685 968 191 1146
Fragments arranged by size:
1146 968 717 685 636 191 75
SfoI GGC'GCC
Cuts at: 1850 2098 1850
Size: 248 4170
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4418
SmaI CCC'GGG
Cuts at: 1440 1440
Size: 4418
SmlI C'TyrA_G
Cuts at: 611 1573 2488 3202 3464 611
Size: 962 915 714 262 1565
Fragments arranged by size:
1565 962 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4418
SphI G_CATG'C
Cuts at: 1471 1971 1471
Size: 500 3918
StyI C'CwwG_G
Cuts at: 107 1552 1906 107
Size: 1445 354 2619
Fragments arranged by size:
2619 1445 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1059 1095 2001 2134 2593 2802 1059
Size: 36 906 133 459 209 2675
Fragments arranged by size:
2675 906 459 209 133 36
TatI w'GTAC_w
Cuts at: 3933 3933
Size: 4418
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 388 420 3935 3967 388
Size: 32 3515 32 839
Fragments arranged by size:
3515 839 32 32
Tth111I GACn'n_nGTC
Cuts at: 2215 2215
Size: 4418
XbaI T'CTAG_A
Cuts at: 1449 1449
Size: 4418
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 948 1053 1100 1913 948
Size: 105 47 813 3453
Fragments arranged by size:
3453 813 105 47
XhoI C'TCGA_G
Cuts at: 611 611
Size: 4418
XmaI C'CCGG_G
Cuts at: 1438 1438
Size: 4418
XmnI GAAnn'nnTTC
Cuts at: 1330 1330
Size: 4418
ZraI GAC'GTC
Cuts at: 252 252
Size: 4418
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AscI AvrII BaeI
BbvCI BfrBI BglI BmgBI BmtI Bpu10I BseRI BsiWI
BsmI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI