Restriction map for
pF1KB5670
(Circular) (Six-base) MAPSORT of: pF1KB5670.seq Check: 1268 from: 1 to: 5888
pF1KB5670 5888 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 547 547
Size: 5888
AccI GT'mk_AC
Cuts at: 2640 2926 2640
Size: 286 5602
Acc65I G'GTAC_C
Cuts at: 2904 2904
Size: 5888
AclI AA'CG_TT
Cuts at: 432 5728 432
Size: 5296 592
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 779 1286 1803 2395 3713 4145 4892 5160 779
Size: 507 517 592 1318 432 747 268 1507
Fragments arranged by size:
1507 1318 747 592 517 507 432 268
AflIII A'CryG_T
Cuts at: 2369 3240 4344 5321 2369
Size: 871 1104 977 2936
Fragments arranged by size:
2936 1104 977 871
AgeI A'CCGG_T
Cuts at: 4276 4276
Size: 5888
AleI CACnn'nnGTG
Cuts at: 2142 2142
Size: 5888
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 298 330 298
Size: 32 5856
AlwNI CAG_nnn'CTG
Cuts at: 2206 2817 3193 4760 2206
Size: 611 376 1567 3334
Fragments arranged by size:
3334 1567 611 376
ApaI G_GGCC'C
Cuts at: 256 256
Size: 5888
ApaLI G'TGCA_C
Cuts at: 4658 4658
Size: 5888
ApoI r'AATT_y
Cuts at: 445 521 780 958 1516 1601 1615 2470 2892
Size: 76 259 178 558 85 14 855 422
Cuts at: 2892 5241 5497 445
Size: 2349 256 836
Fragments arranged by size:
2349 855 836 558 422 259 256 178
85 76 14
AseI AT'TA_AT
Cuts at: 20 503 20
Size: 483 5405
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5888
AvaI C'yCGr_G
Cuts at: 2908 2908
Size: 5888
BamHI G'GATC_C
Cuts at: 1914 2913 1914
Size: 999 4889
BanI G'GyrC_C
Cuts at: 2904 3318 3566 3601 2904
Size: 414 248 35 5191
Fragments arranged by size:
5191 414 248 35
BanII G_rGCy'C
Cuts at: 256 299 2902 3932 256
Size: 43 2603 1030 2212
Fragments arranged by size:
2603 2212 1030 43
BbeI G_GCGC'C
Cuts at: 3322 3570 3322
Size: 248 5640
BbsI GAAGACnn'nnnn_
Cuts at: 166 1949 166
Size: 1783 4105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1566 1600 2693 2727 1566
Size: 34 1093 34 4727
Fragments arranged by size:
4727 1093 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3786 4547 5888 3786
Size: 761 1341 3786
Fragments arranged by size:
3786 1341 761
BclI T'GATC_A
Cuts at: 1262 3408 1262
Size: 2146 3742
BfrBI ATG'CAT
Cuts at: 1667 2158 1667
Size: 491 5397
BglII A'GATC_T
Cuts at: 702 1860 3403 5440 702
Size: 1158 1543 2037 1150
Fragments arranged by size:
2037 1543 1158 1150
BlpI GC'TnA_GC
Cuts at: 1535 3000 1535
Size: 1465 4423
Bme1580I G_kGCm'C
Cuts at: 256 2703 3513 3606 4662 256
Size: 2447 810 93 1056 1482
Fragments arranged by size:
2447 1482 1056 810 93
BmgBI CAC'GTC
Cuts at: 2098 2098
Size: 5888
BmrI ACTGGGnnnn_n'
Cuts at: 1003 1099 2733 3100 3181 3516 5660 1003
Size: 96 1634 367 81 335 2144 1231
Fragments arranged by size:
2144 1634 1231 367 335 96 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4255 4255
Size: 5888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 1103 1465 1474 2825 283
Size: 820 362 9 1351 3346
Fragments arranged by size:
3346 1351 820 362 9
Bpu10I CC'TnA_GC
Cuts at: 184 184
Size: 5888
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 727 3064 3943 4435 4733 265
Size: 462 2337 879 492 298 1420
Fragments arranged by size:
2337 1420 879 492 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 5592 5592
Size: 5888
BsaAI yAC'GTr
Cuts at: 3241 3241
Size: 5888
BsaBI GATnn'nnATC
Cuts at: 6 3402 5439 6
Size: 3396 2037 455
Fragments arranged by size:
3396 2037 455
BsaHI Gr'CG_yC
Cuts at: 3319 3567 4269 5765 3319
Size: 248 702 1496 3442
Fragments arranged by size:
3442 1496 702 248
BsaWI w'CCGG_w
Cuts at: 155 3077 3598 4276 4550 4697 5108 155
Size: 2922 521 678 274 147 411 935
Fragments arranged by size:
2922 935 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 424 424
Size: 5888
BseYI C'CCAG_C
Cuts at: 1918 3313 4071 4648 5144 1918
Size: 1395 758 577 496 2662
Fragments arranged by size:
2662 1395 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 581 241
Size: 340 5548
BsiEI CG_ry'CG
Cuts at: 104 3430 3476 4684 104
Size: 3326 46 1208 1308
Fragments arranged by size:
3326 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 299 900 2022 2902 3680 3870 4662 299
Size: 601 1122 880 778 190 792 1525
Fragments arranged by size:
1525 1122 880 792 778 601 190
BsmI GAATG_Cn'
Cuts at: 583 766 1231 2158 2596 2632 583
Size: 183 465 927 438 36 3839
Fragments arranged by size:
3839 927 465 438 183 36
Bsp1286I G_dGCh'C
Cuts at: 256 299 900 2022 2703 2902 3513 3606 3680
Size: 43 601 1122 681 199 611 93 74
Cuts at: 3680 3870 3932 4662 256
Size: 190 62 730 1482
Fragments arranged by size:
1482 1122 730 681 611 601 199 190
93 74 62 43
BspEI T'CCGG_A
Cuts at: 155 5108 155
Size: 4953 935
BspHI T'CATG_A
Cuts at: 2463 5064 2463
Size: 2601 3287
BspMI ACCTGCnnnn'nnnn_
Cuts at: 337 547 2938 3152 3454 3835 337
Size: 210 2391 214 302 381 2390
Fragments arranged by size:
2391 2390 381 302 214 210
BsrBI CCG'CTC
Cuts at: 50 4180 4234 5718 5883 50
Size: 4130 54 1484 165 55
Fragments arranged by size:
4130 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1830 1929 2222 2271 3800 1830
Size: 99 293 49 1529 3918
Fragments arranged by size:
3918 1529 293 99 49
BsrFI r'CCGG_y
Cuts at: 3886 4067 4276 3886
Size: 181 209 5498
Fragments arranged by size:
5498 209 181
BsrGI T'GTAC_A
Cuts at: 818 2372 818
Size: 1554 4334
BssSI C'ACGA_G
Cuts at: 4159 4517 4159
Size: 358 5530
BstAPI GCAn_nnn'nTGC
Cuts at: 2543 3265 2543
Size: 722 5166
BstBI TT'CG_AA
Cuts at: 4249 4249
Size: 5888
BstXI CCAn_nnnn'nTGG
Cuts at: 1324 1324
Size: 5888
BstYI r'GATC_y
Cuts at: 1 702 753 1004 1297 1860 1914 2913 3380
Size: 701 51 251 293 563 54 999 467
Cuts at: 3380 3403 3738 3984 4996 5082 5094 5440 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 999 701 563 467 449 346 335
293 251 246 86 54 51 23 12
BtgI C'CryG_G
Cuts at: 318 318
Size: 5888
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3838 4019 4182 3838
Size: 181 163 5544
Fragments arranged by size:
5544 181 163
BtsI GCAGTG_nn'
Cuts at: 928 1309 3131 928
Size: 381 1822 3685
Fragments arranged by size:
3685 1822 381
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2151 2184 2186 2219 2151
Size: 33 2 33 5820
Fragments arranged by size:
5820 33 33 2
DraI TTT'AAA
Cuts at: 428 450 831 1521 2036 2887 428
Size: 22 381 690 515 851 3429
Fragments arranged by size:
3429 851 690 515 381 22
DrdI GACnn_nn'nnGTC
Cuts at: 3594 4452 3594
Size: 858 5030
EaeI y'GGCC_r
Cuts at: 3473 3647 4038 4065 5410 3473
Size: 174 391 27 1345 3951
Fragments arranged by size:
3951 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3473 3473
Size: 5888
EarI CTCTTCn'nnn_
Cuts at: 209 1691 1961 2295 3911 4121 209
Size: 1482 270 334 1616 210 1976
Fragments arranged by size:
1976 1616 1482 334 270 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4096 4406 4552 5698 4096
Size: 310 146 1146 4286
Fragments arranged by size:
4286 1146 310 146
EcoICRI GAG'CTC
Cuts at: 297 2900 297
Size: 2603 3285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 779 1103 1286 1465 1474 1803 2395 2825
Size: 496 324 183 179 9 329 592 430
Cuts at: 2825 3713 4145 4892 5160 283
Size: 888 432 747 268 1011
Fragments arranged by size:
1011 888 747 592 496 432 430 329
324 268 183 179 9
EcoNI CCTnn'n_nnAGG
Cuts at: 204 1174 204
Size: 970 4918
EcoO109I rG'GnC_Cy
Cuts at: 252 3027 252
Size: 2775 3113
EcoRI G'AATT_C
Cuts at: 780 2892 780
Size: 2112 3776
EcoRV GAT'ATC
Cuts at: 474 474
Size: 5888
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2646 3907 2646
Size: 1261 4627
FspI TGC'GCA
Cuts at: 3669 3669
Size: 5888
HaeII r_GCGC'y
Cuts at: 3322 3570 4592 5571 3322
Size: 248 1022 979 3639
Fragments arranged by size:
3639 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3730 3760 3762 3792 3730
Size: 30 2 30 5826
Fragments arranged by size:
5826 30 30 2
HincII GTy'rAC
Cuts at: 2927 2927
Size: 5888
KasI G'GCGC_C
Cuts at: 3318 3566 3318
Size: 248 5640
KpnI G_GTAC'C
Cuts at: 2908 2908
Size: 5888
MluI A'CGCG_T
Cuts at: 5321 5321
Size: 5888
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 272 386 4559 4743 272
Size: 114 4173 184 1417
Fragments arranged by size:
4173 1417 184 114
MscI TGG'CCA
Cuts at: 3649 3649
Size: 5888
MslI CAynn'nnrTG
Cuts at: 2122 2142 2275 3877 4004 2122
Size: 20 133 1602 127 4006
Fragments arranged by size:
4006 1602 133 127 20
MspA1I CmG'CkG
Cuts at: 1093 1539 2569 2999 3313 3673 4686 4931 1093
Size: 446 1030 430 314 360 1013 245 2050
Fragments arranged by size:
2050 1030 1013 446 430 360 314 245
NaeI GCC'GGC
Cuts at: 4069 4069
Size: 5888
NarI GG'CG_CC
Cuts at: 3319 3567 3319
Size: 248 5640
NgoMIV G'CCGG_C
Cuts at: 4067 4067
Size: 5888
NsiI A_TGCA'T
Cuts at: 1669 2160 1669
Size: 491 5397
NspI r_CATG'y
Cuts at: 1667 1780 2373 2561 2941 3441 4348 1667
Size: 113 593 188 380 500 907 3207
Fragments arranged by size:
3207 907 593 500 380 188 113
PacI TTA_AT'TAA
Cuts at: 503 503
Size: 5888
PciI A'CATG_T
Cuts at: 2369 4344 2369
Size: 1975 3913
PfoI T'CCnGG_A
Cuts at: 143 299 143
Size: 156 5732
PmeI GTTT'AAAC
Cuts at: 2887 2887
Size: 5888
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5027 5059 5027
Size: 32 5856
PsiI TTA'TAA
Cuts at: 1469 2767 1469
Size: 1298 4590
PspOMI G'GGCC_C
Cuts at: 252 252
Size: 5888
PstI C_TGCA'G
Cuts at: 1892 2539 2716 2935 3620 1892
Size: 647 177 219 685 4160
Fragments arranged by size:
4160 685 647 219 177
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5888
PvuII CAG'CTG
Cuts at: 1093 1539 2569 3313 3673 1093
Size: 446 1030 744 360 3308
Fragments arranged by size:
3308 1030 744 446 360
RsrII CG'GwC_CG
Cuts at: 4083 4083
Size: 5888
SacI G_AGCT'C
Cuts at: 299 2902 299
Size: 2603 3285
SalI G'TCGA_C
Cuts at: 2925 2925
Size: 5888
SapI GCTCTTCn'nnn_
Cuts at: 2295 3911 4121 2295
Size: 1616 210 4062
Fragments arranged by size:
4062 1616 210
SbfI CC_TGCA'GG
Cuts at: 2935 2935
Size: 5888
ScaI AGT'ACT
Cuts at: 5405 5405
Size: 5888
SfcI C'TryA_G
Cuts at: 33 526 902 1888 2535 2712 2931 3616 4609
Size: 493 376 986 647 177 219 685 993
Cuts at: 4609 4800 33
Size: 191 1121
Fragments arranged by size:
1121 993 986 685 647 493 376 219
191 177
SfoI GGC'GCC
Cuts at: 3320 3568 3320
Size: 248 5640
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5888
SmaI CCC'GGG
Cuts at: 2910 2910
Size: 5888
SmlI C'TyrA_G
Cuts at: 280 706 3043 3958 4450 4712 280
Size: 426 2337 915 492 262 1456
Fragments arranged by size:
2337 1456 915 492 426 262
SpeI A'CTAG_T
Cuts at: 67 1237 2332 67
Size: 1170 1095 3623
Fragments arranged by size:
3623 1170 1095
SphI G_CATG'C
Cuts at: 1667 1780 2941 3441 1667
Size: 113 1161 500 4114
Fragments arranged by size:
4114 1161 500 113
SspI AAT'ATT
Cuts at: 2184 2184
Size: 5888
StyI C'CwwG_G
Cuts at: 1376 2552 3022 3376 1376
Size: 1176 470 354 3888
Fragments arranged by size:
3888 1176 470 354
SwaI ATTT'AAAT
Cuts at: 1521 1521
Size: 5888
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3471 3604 4063 4272 3471
Size: 133 459 209 5087
Fragments arranged by size:
5087 459 209 133
TatI w'GTAC_w
Cuts at: 818 2372 2741 2819 5403 818
Size: 1554 369 78 2584 1303
Fragments arranged by size:
2584 1554 1303 369 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2720 2752 5405 5437 2720
Size: 32 2653 32 3171
Fragments arranged by size:
3171 2653 32 32
Tth111I GACn'n_nGTC
Cuts at: 3685 3685
Size: 5888
XbaI T'CTAG_A
Cuts at: 1278 2919 1278
Size: 1641 4247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2022 3383 2022
Size: 1361 4527
XmaI C'CCGG_G
Cuts at: 2908 2908
Size: 5888
XmnI GAAnn'nnTTC
Cuts at: 521 581 649 1483 1813 2444 521
Size: 60 68 834 330 631 3965
Fragments arranged by size:
3965 834 631 330 68 60
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PacI PciI PfoI PmeI PpiI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BaeI
BbvCI BglI BmtI BsaXI BsiWI BsmBI BssHII BstEII
BstZ17I Bsu36I ClaI DraIII FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI PasI PflMI
PmlI PpuMI PshAI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI XhoI ZraI