Restriction map for
pF1KB5664
(Circular) (Six-base) MAPSORT of: pF1KB5664.seq Check: 5534 from: 1 to: 4247
pF1KB5664 4247 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 493 493
Size: 4247
AccI GT'mk_AC
Cuts at: 1285 1285
Size: 4247
Acc65I G'GTAC_C
Cuts at: 1263 1263
Size: 4247
AclI AA'CG_TT
Cuts at: 1241 4087 1241
Size: 2846 1401
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 1204 2072 2504 2857 3519 223
Size: 981 868 432 353 662 951
Fragments arranged by size:
981 951 868 662 432 353
AflIII A'CryG_T
Cuts at: 1599 3399 3680 1599
Size: 1800 281 2166
Fragments arranged by size:
2166 1800 281
AleI CACnn'nnGTG
Cuts at: 235 235
Size: 4247
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1036 1036
Size: 4247
AlwNI CAG_nnn'CTG
Cuts at: 1552 2990 1552
Size: 1438 2809
ApaI G_GGCC'C
Cuts at: 1077 1077
Size: 4247
ApaLI G'TGCA_C
Cuts at: 3085 3085
Size: 4247
ApoI r'AATT_y
Cuts at: 313 1251 3600 3856 313
Size: 938 2349 256 704
Fragments arranged by size:
2349 938 704 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4247
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
AvaI C'yCGr_G
Cuts at: 1267 1267
Size: 4247
BamHI G'GATC_C
Cuts at: 1272 1272
Size: 4247
BanI G'GyrC_C
Cuts at: 560 613 1263 1677 1925 1960 560
Size: 53 650 414 248 35 2847
Fragments arranged by size:
2847 650 414 248 53 35
BanII G_rGCy'C
Cuts at: 1077 1261 2291 1077
Size: 184 1030 3033
Fragments arranged by size:
3033 1030 184
BbeI G_GCGC'C
Cuts at: 617 1681 1929 617
Size: 1064 248 2935
Fragments arranged by size:
2935 1064 248
BbsI GAAGACnn'nnnn_
Cuts at: 418 561 1172 418
Size: 143 611 3493
Fragments arranged by size:
3493 611 143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 246 280 444 478 246
Size: 34 164 34 4015
Fragments arranged by size:
4015 164 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2145 3201 4247 2145
Size: 1056 1046 2145
Fragments arranged by size:
2145 1056 1046
BclI T'GATC_A
Cuts at: 527 1767 527
Size: 1240 3007
BglI GCCn_nnn'nGGC
Cuts at: 475 475
Size: 4247
BglII A'GATC_T
Cuts at: 1762 3799 1762
Size: 2037 2210
BlpI GC'TnA_GC
Cuts at: 1359 1359
Size: 4247
Bme1580I G_kGCm'C
Cuts at: 931 1077 1872 1965 3089 931
Size: 146 795 93 1124 2089
Fragments arranged by size:
2089 1124 795 146 93
BmrI ACTGGGnnnn_n'
Cuts at: 1459 1540 1875 4019 1459
Size: 81 335 2144 1687
Fragments arranged by size:
2144 1687 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 4247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 118
Size: 4247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1423 2302 3016 3314 1423
Size: 879 714 298 2356
Fragments arranged by size:
2356 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 148 3951 148
Size: 3803 444
BsaAI yAC'GTr
Cuts at: 180 1600 180
Size: 1420 2827
BsaBI GATnn'nnATC
Cuts at: 6 295 1761 3798 6
Size: 289 1466 2037 455
Fragments arranged by size:
2037 1466 455 289
BsaHI Gr'CG_yC
Cuts at: 478 548 614 1678 1926 2628 4124 478
Size: 70 66 1064 248 702 1496 601
Fragments arranged by size:
1496 1064 702 601 248 70 66
BsaWI w'CCGG_w
Cuts at: 1436 1957 2635 3046 3193 3467 1436
Size: 521 678 411 147 274 2216
Fragments arranged by size:
2216 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 609 1672 2430 3095 3503 609
Size: 1063 758 665 408 1353
Fragments arranged by size:
1353 1063 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 940 628
Size: 312 3935
BsiEI CG_ry'CG
Cuts at: 104 1789 1835 3065 104
Size: 1685 46 1230 1286
Fragments arranged by size:
1685 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 242 974 1261 2039 2229 3089 242
Size: 732 287 778 190 860 1400
Fragments arranged by size:
1400 860 778 732 287 190
BsmBI CGTCTCn'nnnn_
Cuts at: 136 136
Size: 4247
Bsp1286I G_dGCh'C
Cuts at: 242 931 974 1077 1261 1872 1965 2039 2229
Size: 689 43 103 184 611 93 74 190
Cuts at: 2229 2291 3089 242
Size: 62 798 1400
Fragments arranged by size:
1400 798 689 611 190 184 103 93
74 62 43
BspHI T'CATG_A
Cuts at: 2679 2679
Size: 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 371 493 1178 1297 1511 1813 2194 371
Size: 122 685 119 214 302 381 2424
Fragments arranged by size:
2424 685 381 302 214 122 119
BsrBI CCG'CTC
Cuts at: 50 513 816 2539 2593 4077 4242 50
Size: 463 303 1723 54 1484 165 55
Fragments arranged by size:
1723 1484 463 303 165 55 54
BsrDI GCAATG_nn'
Cuts at: 596 695 2159 596
Size: 99 1464 2684
Fragments arranged by size:
2684 1464 99
BsrFI r'CCGG_y
Cuts at: 138 370 2245 2426 138
Size: 232 1875 181 1959
Fragments arranged by size:
1959 1875 232 181
BssSI C'ACGA_G
Cuts at: 2518 3226 2518
Size: 708 3539
BstAPI GCAn_nnn'nTGC
Cuts at: 1624 1624
Size: 4247
BstBI TT'CG_AA
Cuts at: 2608 2608
Size: 4247
BstEII G'GTnAC_C
Cuts at: 732 732
Size: 4247
BstYI r'GATC_y
Cuts at: 1 1229 1272 1739 1762 2097 2343 2649 2661
Size: 1228 43 467 23 335 246 306 12
Cuts at: 2661 2747 3799 1
Size: 86 1052 449
Fragments arranged by size:
1228 1052 467 449 335 306 246 86
43 23 12
Bsu36I CC'TnA_GG
Cuts at: 445 445
Size: 4247
BtgI C'CryG_G
Cuts at: 107 431 821 839 107
Size: 324 390 18 3515
Fragments arranged by size:
3515 390 324 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 360 486 849 2197 2378 2541 360
Size: 126 363 1348 181 163 2066
Fragments arranged by size:
2066 1348 363 181 163 126
BtsI GCAGTG_nn'
Cuts at: 826 1490 826
Size: 664 3583
DraI TTT'AAA
Cuts at: 1246 1246
Size: 4247
DrdI GACnn_nn'nnGTC
Cuts at: 1953 3297 1953
Size: 1344 2903
EaeI y'GGCC_r
Cuts at: 368 497 722 794 1007 1832 2006 2397 2424
Size: 129 225 72 213 825 174 391 27
Cuts at: 2424 3769 368
Size: 1345 846
Fragments arranged by size:
1345 846 825 391 225 213 174 129
72 27
EagI C'GGCC_G
Cuts at: 1832 1832
Size: 4247
EarI CTCTTCn'nnn_
Cuts at: 247 889 898 943 2270 2480 247
Size: 642 9 45 1327 210 2014
Fragments arranged by size:
2014 1327 642 210 45 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 969 2455 3197 3343 4057 969
Size: 1486 742 146 714 1159
Fragments arranged by size:
1486 1159 742 714 146
EcoICRI GAG'CTC
Cuts at: 1259 1259
Size: 4247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 223 1204 2072 2504 2857 3519 118
Size: 105 981 868 432 353 662 846
Fragments arranged by size:
981 868 846 662 432 353 105
EcoNI CCTnn'n_nnAGG
Cuts at: 448 940 448
Size: 492 3755
EcoO109I rG'GnC_Cy
Cuts at: 1073 1386 1073
Size: 313 3934
EcoRI G'AATT_C
Cuts at: 1251 1251
Size: 4247
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 4247
FspI TGC'GCA
Cuts at: 2028 2028
Size: 4247
HaeII r_GCGC'y
Cuts at: 617 1681 1929 3159 3930 617
Size: 1064 248 1230 771 934
Fragments arranged by size:
1230 1064 934 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2089 2119 2121 2151 2089
Size: 30 2 30 4185
Fragments arranged by size:
4185 30 30 2
HincII GTy'rAC
Cuts at: 390 1286 390
Size: 896 3351
KasI G'GCGC_C
Cuts at: 613 1677 1925 613
Size: 1064 248 2935
Fragments arranged by size:
2935 1064 248
KpnI G_GTAC'C
Cuts at: 1267 1267
Size: 4247
MluI A'CGCG_T
Cuts at: 3680 3680
Size: 4247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3006 3190 3006
Size: 184 4063
MscI TGG'CCA
Cuts at: 499 796 1009 2008 499
Size: 297 213 999 2738
Fragments arranged by size:
2738 999 297 213
MslI CAynn'nnrTG
Cuts at: 235 397 730 904 2236 2363 235
Size: 162 333 174 1332 127 2119
Fragments arranged by size:
2119 1332 333 174 162 127
MspA1I CmG'CkG
Cuts at: 1358 1672 2032 2816 3061 1358
Size: 314 360 784 245 2544
Fragments arranged by size:
2544 784 360 314 245
NaeI GCC'GGC
Cuts at: 372 2428 372
Size: 2056 2191
NarI GG'CG_CC
Cuts at: 614 1678 1926 614
Size: 1064 248 2935
Fragments arranged by size:
2935 1064 248
NcoI C'CATG_G
Cuts at: 107 821 107
Size: 714 3533
NgoMIV G'CCGG_C
Cuts at: 370 2426 370
Size: 2056 2191
NspI r_CATG'y
Cuts at: 330 1300 1800 3403 330
Size: 970 500 1603 1174
Fragments arranged by size:
1603 1174 970 500
PciI A'CATG_T
Cuts at: 3399 3399
Size: 4247
PflMI CCAn_nnn'nTGG
Cuts at: 180 437 180
Size: 257 3990
PmeI GTTT'AAAC
Cuts at: 1246 1246
Size: 4247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2693 2725 2693
Size: 32 4215
PspOMI G'GGCC_C
Cuts at: 1073 1073
Size: 4247
PstI C_TGCA'G
Cuts at: 259 490 916 1294 1979 259
Size: 231 426 378 685 2527
Fragments arranged by size:
2527 685 426 378 231
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4247
PvuII CAG'CTG
Cuts at: 1672 2032 1672
Size: 360 3887
RsrII CG'GwC_CG
Cuts at: 836 2442 836
Size: 1606 2641
SacI G_AGCT'C
Cuts at: 1261 1261
Size: 4247
SalI G'TCGA_C
Cuts at: 1284 1284
Size: 4247
SapI GCTCTTCn'nnn_
Cuts at: 247 2270 2480 247
Size: 2023 210 2014
Fragments arranged by size:
2023 2014 210
SbfI CC_TGCA'GG
Cuts at: 916 1294 916
Size: 378 3869
ScaI AGT'ACT
Cuts at: 3764 3764
Size: 4247
SfcI C'TryA_G
Cuts at: 33 255 486 912 1290 1975 2943 3134 33
Size: 222 231 426 378 685 968 191 1146
Fragments arranged by size:
1146 968 685 426 378 231 222 191
SfoI GGC'GCC
Cuts at: 615 1679 1927 615
Size: 1064 248 2935
Fragments arranged by size:
2935 1064 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
SmaI CCC'GGG
Cuts at: 1269 1269
Size: 4247
SmlI C'TyrA_G
Cuts at: 1402 2317 3031 3293 1402
Size: 915 714 262 2356
Fragments arranged by size:
2356 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4247
SphI G_CATG'C
Cuts at: 1300 1800 1300
Size: 500 3747
StuI AGG'CCT
Cuts at: 211 540 211
Size: 329 3918
StyI C'CwwG_G
Cuts at: 107 471 821 1381 1735 107
Size: 364 350 560 354 2619
Fragments arranged by size:
2619 560 364 354 350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1101 1830 1963 2422 2631 1101
Size: 729 133 459 209 2717
Fragments arranged by size:
2717 729 459 209 133
TatI w'GTAC_w
Cuts at: 329 692 3762 329
Size: 363 3070 814
Fragments arranged by size:
3070 814 363
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 872 904 3764 3796 872
Size: 32 2860 32 1323
Fragments arranged by size:
2860 1323 32 32
Tth111I GACn'n_nGTC
Cuts at: 2044 2044
Size: 4247
XbaI T'CTAG_A
Cuts at: 1278 1278
Size: 4247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1742 1742
Size: 4247
XmaI C'CCGG_G
Cuts at: 1267 1267
Size: 4247
XmnI GAAnn'nnTTC
Cuts at: 415 415
Size: 4247
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BseRI
BsiWI BsmI BspEI BsrGI BssHII BstXI BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI