Restriction map for
pF1KB5659
(Circular) (Six-base) MAPSORT of: pF1KB5659.seq Check: 7308 from: 1 to: 4037
pF1KB5659 4037 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AatII G_ACGT'C
Cuts at: 477 477
Size: 4037
AccI GT'mk_AC
Cuts at: 1075 1075
Size: 4037
Acc65I G'GTAC_C
Cuts at: 1053 1053
Size: 4037
AclI AA'CG_TT
Cuts at: 3877 3877
Size: 4037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 1862 2294 3041 3309 634
Size: 1228 432 747 268 1362
Fragments arranged by size:
1362 1228 747 432 268
AflIII A'CryG_T
Cuts at: 1389 2493 3470 1389
Size: 1104 977 1956
Fragments arranged by size:
1956 1104 977
AgeI A'CCGG_T
Cuts at: 2425 2425
Size: 4037
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 368 368
Size: 4037
AlwNI CAG_nnn'CTG
Cuts at: 492 1342 2909 492
Size: 850 1567 1620
Fragments arranged by size:
1620 1567 850
ApaI G_GGCC'C
Cuts at: 933 933
Size: 4037
ApaLI G'TGCA_C
Cuts at: 2807 2807
Size: 4037
ApoI r'AATT_y
Cuts at: 607 1041 3390 3646 607
Size: 434 2349 256 998
Fragments arranged by size:
2349 998 434 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4037
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
AvaI C'yCGr_G
Cuts at: 1057 1057
Size: 4037
BamHI G'GATC_C
Cuts at: 367 1062 367
Size: 695 3342
BanI G'GyrC_C
Cuts at: 1053 1467 1715 1750 1053
Size: 414 248 35 3340
Fragments arranged by size:
3340 414 248 35
BanII G_rGCy'C
Cuts at: 470 933 977 1051 2081 470
Size: 463 44 74 1030 2426
Fragments arranged by size:
2426 1030 463 74 44
BbeI G_GCGC'C
Cuts at: 1471 1719 1471
Size: 248 3789
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 338 372 338
Size: 34 4003
BciVI GTATCCnnnnn_n'
Cuts at: 668 1935 2696 4037 668
Size: 1267 761 1341 668
Fragments arranged by size:
1341 1267 761 668
BclI T'GATC_A
Cuts at: 1557 1557
Size: 4037
BglII A'GATC_T
Cuts at: 194 1552 3589 194
Size: 1358 2037 642
Fragments arranged by size:
2037 1358 642
BlpI GC'TnA_GC
Cuts at: 1149 1149
Size: 4037
Bme1580I G_kGCm'C
Cuts at: 810 933 1662 1755 2811 810
Size: 123 729 93 1056 2036
Fragments arranged by size:
2036 1056 729 123 93
BmrI ACTGGGnnnn_n'
Cuts at: 430 1249 1330 1665 3809 430
Size: 819 81 335 2144 658
Fragments arranged by size:
2144 819 658 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 4037
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 585 832 585
Size: 247 3790
Bpu10I CC'TnA_GC
Cuts at: 178 685 178
Size: 507 3530
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 1213 2092 2584 2882 748
Size: 465 879 492 298 1903
Fragments arranged by size:
1903 879 492 465 298
BsaI GGTCTCn'nnnn_
Cuts at: 835 3741 835
Size: 2906 1131
BsaAI yAC'GTr
Cuts at: 1390 1390
Size: 4037
BsaBI GATnn'nnATC
Cuts at: 6 1551 3588 6
Size: 1545 2037 455
Fragments arranged by size:
2037 1545 455
BsaHI Gr'CG_yC
Cuts at: 474 1468 1716 2418 3914 474
Size: 994 248 702 1496 597
Fragments arranged by size:
1496 994 702 597 248
BsaWI w'CCGG_w
Cuts at: 1226 1747 2425 2699 2846 3257 1226
Size: 521 678 274 147 411 2006
Fragments arranged by size:
2006 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 961 964 961
Size: 3 4034
BseYI C'CCAG_C
Cuts at: 1462 2220 2797 3293 1462
Size: 758 577 496 2206
Fragments arranged by size:
2206 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 254
Size: 4037
BsiEI CG_ry'CG
Cuts at: 104 152 225 1579 1625 2833 104
Size: 48 73 1354 46 1208 1308
Fragments arranged by size:
1354 1308 1208 73 48 46
BsiHKAI G_wGCw'C
Cuts at: 470 977 1021 1051 1829 2019 2811 470
Size: 507 44 30 778 190 792 1696
Fragments arranged by size:
1696 792 778 507 190 44 30
Bsp1286I G_dGCh'C
Cuts at: 470 810 933 977 1021 1051 1662 1755 1829
Size: 340 123 44 44 30 611 93 74
Cuts at: 1829 2019 2081 2811 470
Size: 190 62 730 1696
Fragments arranged by size:
1696 730 611 340 190 123 93 74
62 44 44 30
BspEI T'CCGG_A
Cuts at: 3257 3257
Size: 4037
BspHI T'CATG_A
Cuts at: 3213 3213
Size: 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1087 1301 1603 1984 1087
Size: 214 302 381 3140
Fragments arranged by size:
3140 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2329 2383 3867 4032 50
Size: 2279 54 1484 165 55
Fragments arranged by size:
2279 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 283 1949 283
Size: 1666 2371
BsrFI r'CCGG_y
Cuts at: 220 2035 2216 2425 220
Size: 1815 181 209 1832
Fragments arranged by size:
1832 1815 209 181
BsrGI T'GTAC_A
Cuts at: 892 892
Size: 4037
BssSI C'ACGA_G
Cuts at: 2308 2666 2308
Size: 358 3679
BstAPI GCAn_nnn'nTGC
Cuts at: 1414 1414
Size: 4037
BstBI TT'CG_AA
Cuts at: 2398 2398
Size: 4037
BstXI CCAn_nnnn'nTGG
Cuts at: 518 518
Size: 4037
BstYI r'GATC_y
Cuts at: 1 194 367 1062 1529 1552 1887 2133 3145
Size: 193 173 695 467 23 335 246 1012
Cuts at: 3145 3231 3243 3589 1
Size: 86 12 346 449
Fragments arranged by size:
1012 695 467 449 346 335 246 193
173 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1987 2168 2331 1987
Size: 181 163 3693
Fragments arranged by size:
3693 181 163
BtsI GCAGTG_nn'
Cuts at: 396 1280 396
Size: 884 3153
DraI TTT'AAA
Cuts at: 1036 1036
Size: 4037
DrdI GACnn_nn'nnGTC
Cuts at: 1743 2601 1743
Size: 858 3179
EaeI y'GGCC_r
Cuts at: 222 1622 1796 2187 2214 3559 222
Size: 1400 174 391 27 1345 700
Fragments arranged by size:
1400 1345 700 391 174 27
EagI C'GGCC_G
Cuts at: 222 1622 222
Size: 1400 2637
EarI CTCTTCn'nnn_
Cuts at: 167 2060 2270 167
Size: 1893 210 1934
Fragments arranged by size:
1934 1893 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2245 2555 2701 3847 2245
Size: 310 146 1146 2435
Fragments arranged by size:
2435 1146 310 146
EcoICRI GAG'CTC
Cuts at: 468 975 1049 468
Size: 507 74 3456
Fragments arranged by size:
3456 507 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 585 634 832 1862 2294 3041 3309 585
Size: 49 198 1030 432 747 268 1313
Fragments arranged by size:
1313 1030 747 432 268 198 49
EcoO109I rG'GnC_Cy
Cuts at: 929 1176 929
Size: 247 3790
EcoRI G'AATT_C
Cuts at: 607 1041 607
Size: 434 3603
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2056 2056
Size: 4037
FspI TGC'GCA
Cuts at: 1818 1818
Size: 4037
HaeII r_GCGC'y
Cuts at: 1471 1719 2741 3720 1471
Size: 248 1022 979 1788
Fragments arranged by size:
1788 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 359 391 397 429 464 496 1879 1909 1911
Size: 32 6 32 35 32 1383 30 2
Cuts at: 1911 1941 359
Size: 30 2455
Fragments arranged by size:
2455 1383 35 32 32 32 30 30
6 2
HincII GTy'rAC
Cuts at: 1076 1076
Size: 4037
KasI G'GCGC_C
Cuts at: 1467 1715 1467
Size: 248 3789
KpnI G_GTAC'C
Cuts at: 1057 1057
Size: 4037
MluI A'CGCG_T
Cuts at: 3470 3470
Size: 4037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 479 495 2708 2892 479
Size: 16 2213 184 1624
Fragments arranged by size:
2213 1624 184 16
MscI TGG'CCA
Cuts at: 1798 1798
Size: 4037
MslI CAynn'nnrTG
Cuts at: 350 2026 2153 350
Size: 1676 127 2234
Fragments arranged by size:
2234 1676 127
MspA1I CmG'CkG
Cuts at: 863 997 1148 1462 1822 2835 3080 863
Size: 134 151 314 360 1013 245 1820
Fragments arranged by size:
1820 1013 360 314 245 151 134
NaeI GCC'GGC
Cuts at: 222 2218 222
Size: 1996 2041
NarI GG'CG_CC
Cuts at: 1468 1716 1468
Size: 248 3789
NgoMIV G'CCGG_C
Cuts at: 220 2216 220
Size: 1996 2041
NspI r_CATG'y
Cuts at: 1090 1590 2497 1090
Size: 500 907 2630
Fragments arranged by size:
2630 907 500
PciI A'CATG_T
Cuts at: 2493 2493
Size: 4037
PflMI CCAn_nnn'nTGG
Cuts at: 492 492
Size: 4037
PfoI T'CCnGG_A
Cuts at: 968 968
Size: 4037
PmeI GTTT'AAAC
Cuts at: 1036 1036
Size: 4037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 4005
PspOMI G'GGCC_C
Cuts at: 929 929
Size: 4037
PstI C_TGCA'G
Cuts at: 1084 1769 1084
Size: 685 3352
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4037
PvuII CAG'CTG
Cuts at: 997 1462 1822 997
Size: 465 360 3212
Fragments arranged by size:
3212 465 360
RsrII CG'GwC_CG
Cuts at: 2232 2232
Size: 4037
SacI G_AGCT'C
Cuts at: 470 977 1051 470
Size: 507 74 3456
Fragments arranged by size:
3456 507 74
SalI G'TCGA_C
Cuts at: 1074 1074
Size: 4037
SapI GCTCTTCn'nnn_
Cuts at: 167 2060 2270 167
Size: 1893 210 1934
Fragments arranged by size:
1934 1893 210
SbfI CC_TGCA'GG
Cuts at: 1084 1084
Size: 4037
ScaI AGT'ACT
Cuts at: 3554 3554
Size: 4037
SfcI C'TryA_G
Cuts at: 33 540 1080 1765 2758 2949 33
Size: 507 540 685 993 191 1121
Fragments arranged by size:
1121 993 685 540 507 191
SfoI GGC'GCC
Cuts at: 1469 1717 1469
Size: 248 3789
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
SmaI CCC'GGG
Cuts at: 1059 1059
Size: 4037
SmlI C'TyrA_G
Cuts at: 763 1192 2107 2599 2861 763
Size: 429 915 492 262 1939
Fragments arranged by size:
1939 915 492 429 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4037
SphI G_CATG'C
Cuts at: 1090 1590 1090
Size: 500 3537
SspI AAT'ATT
Cuts at: 648 648
Size: 4037
StuI AGG'CCT
Cuts at: 297 297
Size: 4037
StyI C'CwwG_G
Cuts at: 1171 1525 1171
Size: 354 3683
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 859 1620 1753 2212 2421 859
Size: 761 133 459 209 2475
Fragments arranged by size:
2475 761 459 209 133
TatI w'GTAC_w
Cuts at: 892 3552 892
Size: 2660 1377
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3554 3586 3554
Size: 32 4005
Tth111I GACn'n_nGTC
Cuts at: 1834 1834
Size: 4037
XbaI T'CTAG_A
Cuts at: 1068 1068
Size: 4037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1532 1532
Size: 4037
XmaI C'CCGG_G
Cuts at: 1057 1057
Size: 4037
ZraI GAC'GTC
Cuts at: 475 475
Size: 4037
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI XmnI