Restriction map for
pF1KB5646
(Circular) (Six-base) MAPSORT of: pF1KB5646.seq Check: 1056 from: 1 to: 4577
pF1KB5646 4577 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 1615 1615
Size: 4577
Acc65I G'GTAC_C
Cuts at: 1593 1593
Size: 4577
AclI AA'CG_TT
Cuts at: 4417 4417
Size: 4577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1385 2402 2834 3581 3849 1385
Size: 1017 432 747 268 2113
Fragments arranged by size:
2113 1017 747 432 268
AflII C'TTAA_G
Cuts at: 822 822
Size: 4577
AflIII A'CryG_T
Cuts at: 438 1929 3033 4010 438
Size: 1491 1104 977 1005
Fragments arranged by size:
1491 1104 1005 977
AgeI A'CCGG_T
Cuts at: 2965 2965
Size: 4577
AlwNI CAG_nnn'CTG
Cuts at: 1882 3449 1882
Size: 1567 3010
ApaLI G'TGCA_C
Cuts at: 3347 3347
Size: 4577
ApoI r'AATT_y
Cuts at: 290 497 808 1581 3930 4186 290
Size: 207 311 773 2349 256 681
Fragments arranged by size:
2349 773 681 311 256 207
AseI AT'TA_AT
Cuts at: 20 737 1030 20
Size: 717 293 3567
Fragments arranged by size:
3567 717 293
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
AvaI C'yCGr_G
Cuts at: 139 157 187 1556 1597 139
Size: 18 30 1369 41 3119
Fragments arranged by size:
3119 1369 41 30 18
BamHI G'GATC_C
Cuts at: 1158 1602 1158
Size: 444 4133
BanI G'GyrC_C
Cuts at: 570 1593 2007 2255 2290 570
Size: 1023 414 248 35 2857
Fragments arranged by size:
2857 1023 414 248 35
BanII G_rGCy'C
Cuts at: 334 1591 2621 334
Size: 1257 1030 2290
Fragments arranged by size:
2290 1257 1030
BbeI G_GCGC'C
Cuts at: 2011 2259 2011
Size: 248 4329
BciVI GTATCCnnnnn_n'
Cuts at: 2475 3236 4577 2475
Size: 761 1341 2475
Fragments arranged by size:
2475 1341 761
BclI T'GATC_A
Cuts at: 2097 2097
Size: 4577
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4577
BglII A'GATC_T
Cuts at: 2092 4129 2092
Size: 2037 2540
BlpI GC'TnA_GC
Cuts at: 178 1689 178
Size: 1511 3066
Bme1580I G_kGCm'C
Cuts at: 2202 2295 3351 2202
Size: 93 1056 3428
Fragments arranged by size:
3428 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1789 1870 2205 4349 1789
Size: 81 335 2144 2017
Fragments arranged by size:
2144 2017 335 81
BmtI G_CTAG'C
Cuts at: 487 487
Size: 4577
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 234 2944 234
Size: 2710 1867
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 510 989 416
Size: 94 479 4004
Fragments arranged by size:
4004 479 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 771 1753 2632 3124 3422 353
Size: 418 982 879 492 298 1508
Fragments arranged by size:
1508 982 879 492 418 298
BsaI GGTCTCn'nnnn_
Cuts at: 4281 4281
Size: 4577
BsaAI yAC'GTr
Cuts at: 1930 1930
Size: 4577
BsaBI GATnn'nnATC
Cuts at: 6 2091 4128 6
Size: 2085 2037 455
Fragments arranged by size:
2085 2037 455
BsaHI Gr'CG_yC
Cuts at: 2008 2256 2958 4454 2008
Size: 248 702 1496 2131
Fragments arranged by size:
2131 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1766 2287 2965 3239 3386 3797 1766
Size: 521 678 274 147 411 2546
Fragments arranged by size:
2546 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1082 1112 1082
Size: 30 4547
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1207 1415 1207
Size: 208 4369
BseYI C'CCAG_C
Cuts at: 843 2002 2760 3337 3833 843
Size: 1159 758 577 496 1587
Fragments arranged by size:
1587 1159 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 855 932 1166 855
Size: 77 234 4266
Fragments arranged by size:
4266 234 77
BsiEI CG_ry'CG
Cuts at: 104 2119 2165 3373 104
Size: 2015 46 1208 1308
Fragments arranged by size:
2015 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 179 1591 2369 2559 3351 179
Size: 1412 778 190 792 1405
Fragments arranged by size:
1412 1405 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 179 334 1591 2202 2295 2369 2559 2621 3351
Size: 155 1257 611 93 74 190 62 730
Cuts at: 3351 179
Size: 1405
Fragments arranged by size:
1405 1257 730 611 190 155 93 74
62
BspEI T'CCGG_A
Cuts at: 3797 3797
Size: 4577
BspHI T'CATG_A
Cuts at: 3753 3753
Size: 4577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1627 1841 2143 2524 1627
Size: 214 302 381 3680
Fragments arranged by size:
3680 381 302 214
BsrBI CCG'CTC
Cuts at: 50 120 2869 2923 4407 4572 50
Size: 70 2749 54 1484 165 55
Fragments arranged by size:
2749 1484 165 70 55 54
BsrDI GCAATG_nn'
Cuts at: 2489 2489
Size: 4577
BsrFI r'CCGG_y
Cuts at: 1538 2575 2756 2965 1538
Size: 1037 181 209 3150
Fragments arranged by size:
3150 1037 209 181
BsrGI T'GTAC_A
Cuts at: 921 921
Size: 4577
BssHII G'CGCG_C
Cuts at: 201 201
Size: 4577
BssSI C'ACGA_G
Cuts at: 2848 3206 2848
Size: 358 4219
BstAPI GCAn_nnn'nTGC
Cuts at: 1954 1954
Size: 4577
BstBI TT'CG_AA
Cuts at: 2938 2938
Size: 4577
BstYI r'GATC_y
Cuts at: 1 1158 1306 1602 2069 2092 2427 2673 3685
Size: 1157 148 296 467 23 335 246 1012
Cuts at: 3685 3771 3783 4129 1
Size: 86 12 346 449
Fragments arranged by size:
1157 1012 467 449 346 335 296 246
148 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1520 1520
Size: 4577
BtgI C'CryG_G
Cuts at: 107 107
Size: 4577
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2527 2708 2871 2527
Size: 181 163 4233
Fragments arranged by size:
4233 181 163
BtsI GCAGTG_nn'
Cuts at: 867 1820 867
Size: 953 3624
DraI TTT'AAA
Cuts at: 434 1576 434
Size: 1142 3435
DrdI GACnn_nn'nnGTC
Cuts at: 2283 3141 2283
Size: 858 3719
EaeI y'GGCC_r
Cuts at: 1225 2162 2336 2727 2754 4099 1225
Size: 937 174 391 27 1345 1703
Fragments arranged by size:
1703 1345 937 391 174 27
EagI C'GGCC_G
Cuts at: 2162 2162
Size: 4577
EarI CTCTTCn'nnn_
Cuts at: 222 825 2600 2810 222
Size: 603 1775 210 1989
Fragments arranged by size:
1989 1775 603 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 130 2785 3095 3241 4387 130
Size: 2655 310 146 1146 320
Fragments arranged by size:
2655 1146 320 310 146
EcoICRI GAG'CTC
Cuts at: 1589 1589
Size: 4577
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 510 989 1385 2402 2834 3581 3849 416
Size: 94 479 396 1017 432 747 268 1144
Fragments arranged by size:
1144 1017 747 479 432 396 268 94
EcoNI CCTnn'n_nnAGG
Cuts at: 704 1523 704
Size: 819 3758
EcoO109I rG'GnC_Cy
Cuts at: 1716 1716
Size: 4577
EcoRI G'AATT_C
Cuts at: 1581 1581
Size: 4577
EcoRV GAT'ATC
Cuts at: 602 602
Size: 4577
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 679 2596 679
Size: 1917 2660
FspI TGC'GCA
Cuts at: 2358 2358
Size: 4577
HaeII r_GCGC'y
Cuts at: 2011 2259 3281 4260 2011
Size: 248 1022 979 2328
Fragments arranged by size:
2328 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 395 427 1291 1323 1503 1535 2419 2449 2451
Size: 32 864 32 180 32 884 30 2
Cuts at: 2451 2481 395
Size: 30 2491
Fragments arranged by size:
2491 884 864 180 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1616 1616
Size: 4577
KasI G'GCGC_C
Cuts at: 2007 2255 2007
Size: 248 4329
KpnI G_GTAC'C
Cuts at: 1597 1597
Size: 4577
MluI A'CGCG_T
Cuts at: 4010 4010
Size: 4577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 539 832 1245 3248 3432 539
Size: 293 413 2003 184 1684
Fragments arranged by size:
2003 1684 413 293 184
MscI TGG'CCA
Cuts at: 1227 2338 1227
Size: 1111 3466
MslI CAynn'nnrTG
Cuts at: 2566 2693 2566
Size: 127 4450
MspA1I CmG'CkG
Cuts at: 182 1688 2002 2362 3375 3620 182
Size: 1506 314 360 1013 245 1139
Fragments arranged by size:
1506 1139 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2758 2758
Size: 4577
NarI GG'CG_CC
Cuts at: 2008 2256 2008
Size: 248 4329
NcoI C'CATG_G
Cuts at: 107 107
Size: 4577
NdeI CA'TA_TG
Cuts at: 579 579
Size: 4577
NgoMIV G'CCGG_C
Cuts at: 2756 2756
Size: 4577
NheI G'CTAG_C
Cuts at: 483 483
Size: 4577
NspI r_CATG'y
Cuts at: 1630 2130 3037 1630
Size: 500 907 3170
Fragments arranged by size:
3170 907 500
PciI A'CATG_T
Cuts at: 3033 3033
Size: 4577
PflMI CCAn_nnn'nTGG
Cuts at: 394 394
Size: 4577
PfoI T'CCnGG_A
Cuts at: 251 251
Size: 4577
PmeI GTTT'AAAC
Cuts at: 1576 1576
Size: 4577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 3716
Size: 32 4545
PsiI TTA'TAA
Cuts at: 296 296
Size: 4577
PstI C_TGCA'G
Cuts at: 226 1624 2309 226
Size: 1398 685 2494
Fragments arranged by size:
2494 1398 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4577
PvuII CAG'CTG
Cuts at: 2002 2362 2002
Size: 360 4217
RsrII CG'GwC_CG
Cuts at: 2772 2772
Size: 4577
SacI G_AGCT'C
Cuts at: 1591 1591
Size: 4577
SalI G'TCGA_C
Cuts at: 1614 1614
Size: 4577
SapI GCTCTTCn'nnn_
Cuts at: 222 2600 2810 222
Size: 2378 210 1989
Fragments arranged by size:
2378 1989 210
SbfI CC_TGCA'GG
Cuts at: 1624 1624
Size: 4577
ScaI AGT'ACT
Cuts at: 4094 4094
Size: 4577
SfcI C'TryA_G
Cuts at: 33 222 1620 2305 3298 3489 33
Size: 189 1398 685 993 191 1121
Fragments arranged by size:
1398 1121 993 685 191 189
SfoI GGC'GCC
Cuts at: 2009 2257 2009
Size: 248 4329
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
SmaI CCC'GGG
Cuts at: 141 159 189 1599 141
Size: 18 30 1410 3119
Fragments arranged by size:
3119 1410 30 18
SmlI C'TyrA_G
Cuts at: 368 786 822 1732 2647 3139 3401 368
Size: 418 36 910 915 492 262 1544
Fragments arranged by size:
1544 915 910 492 418 262 36
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4577
SphI G_CATG'C
Cuts at: 1630 2130 1630
Size: 500 4077
StuI AGG'CCT
Cuts at: 1389 1389
Size: 4577
StyI C'CwwG_G
Cuts at: 107 683 1711 2065 107
Size: 576 1028 354 2619
Fragments arranged by size:
2619 1028 576 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2160 2293 2752 2961 2160
Size: 133 459 209 3776
Fragments arranged by size:
3776 459 209 133
TatI w'GTAC_w
Cuts at: 760 921 4092 760
Size: 161 3171 1245
Fragments arranged by size:
3171 1245 161
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 846 878 4094 4126 846
Size: 32 3216 32 1297
Fragments arranged by size:
3216 1297 32 32
Tth111I GACn'n_nGTC
Cuts at: 2374 2374
Size: 4577
XbaI T'CTAG_A
Cuts at: 1608 1608
Size: 4577
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1347 2072 1347
Size: 725 3852
XmaI C'CCGG_G
Cuts at: 139 157 187 1597 139
Size: 18 30 1410 3119
Fragments arranged by size:
3119 1410 30 18
XmnI GAAnn'nnTTC
Cuts at: 497 497
Size: 4577
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NspI PciI
PflMI PfoI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
Bpu10I BsiWI BsmI BsmBI BstEII BstXI BstZ17I ClaI
CspCI DraIII FseI FspAI HindIII HpaI MfeI NotI
NruI NsiI PacI PasI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI