Restriction map for
pF1KB5635
(Circular) (Six-base) MAPSORT of: pF1KB5635.seq Check: 3676 from: 1 to: 4535
pF1KB5635 4535 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 1573 1573
Size: 4535
Acc65I G'GTAC_C
Cuts at: 1551 1551
Size: 4535
AclI AA'CG_TT
Cuts at: 4375 4375
Size: 4535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2360 2792 3539 3807 2360
Size: 432 747 268 3088
Fragments arranged by size:
3088 747 432 268
AflIII A'CryG_T
Cuts at: 1887 2991 3968 1887
Size: 1104 977 2454
Fragments arranged by size:
2454 1104 977
AgeI A'CCGG_T
Cuts at: 2923 2923
Size: 4535
AlwNI CAG_nnn'CTG
Cuts at: 1840 3407 1840
Size: 1567 2968
ApaLI G'TGCA_C
Cuts at: 3305 3305
Size: 4535
ApoI r'AATT_y
Cuts at: 1539 3888 4144 1539
Size: 2349 256 1930
Fragments arranged by size:
2349 1930 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4535
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
AvaI C'yCGr_G
Cuts at: 1555 1555
Size: 4535
BamHI G'GATC_C
Cuts at: 1560 1560
Size: 4535
BanI G'GyrC_C
Cuts at: 371 1551 1965 2213 2248 371
Size: 1180 414 248 35 2658
Fragments arranged by size:
2658 1180 414 248 35
BanII G_rGCy'C
Cuts at: 1549 2579 1549
Size: 1030 3505
BbeI G_GCGC'C
Cuts at: 1969 2217 1969
Size: 248 4287
BbsI GAAGACnn'nnnn_
Cuts at: 795 795
Size: 4535
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 846 880 846
Size: 34 4501
BciVI GTATCCnnnnn_n'
Cuts at: 1202 2433 3194 4535 1202
Size: 1231 761 1341 1202
Fragments arranged by size:
1341 1231 1202 761
BclI T'GATC_A
Cuts at: 1373 2055 1373
Size: 682 3853
BfrBI ATG'CAT
Cuts at: 624 624
Size: 4535
BglII A'GATC_T
Cuts at: 2050 4087 2050
Size: 2037 2498
BlpI GC'TnA_GC
Cuts at: 1647 1647
Size: 4535
Bme1580I G_kGCm'C
Cuts at: 376 2160 2253 3309 376
Size: 1784 93 1056 1602
Fragments arranged by size:
1784 1602 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 821 1747 1828 2163 4307 821
Size: 926 81 335 2144 1049
Fragments arranged by size:
2144 1049 926 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 4535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1243 1243
Size: 4535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1297 1711 2590 3082 3380 1297
Size: 414 879 492 298 2452
Fragments arranged by size:
2452 879 492 414 298
BsaI GGTCTCn'nnnn_
Cuts at: 4239 4239
Size: 4535
BsaAI yAC'GTr
Cuts at: 1888 1888
Size: 4535
BsaBI GATnn'nnATC
Cuts at: 6 1378 2049 4086 6
Size: 1372 671 2037 455
Fragments arranged by size:
2037 1372 671 455
BsaHI Gr'CG_yC
Cuts at: 1966 2214 2916 4412 1966
Size: 248 702 1496 2089
Fragments arranged by size:
2089 1496 702 248
BsaWI w'CCGG_w
Cuts at: 656 1724 2245 2923 3197 3344 3755 656
Size: 1068 521 678 274 147 411 1436
Fragments arranged by size:
1436 1068 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 287 287
Size: 4535
BseYI C'CCAG_C
Cuts at: 1960 2718 3295 3791 1960
Size: 758 577 496 2704
Fragments arranged by size:
2704 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 399 572 660 280
Size: 119 173 88 4155
Fragments arranged by size:
4155 173 119 88
BsiEI CG_ry'CG
Cuts at: 104 2077 2123 3331 104
Size: 1973 46 1208 1308
Fragments arranged by size:
1973 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1549 2327 2517 3309 1549
Size: 778 190 792 2775
Fragments arranged by size:
2775 792 778 190
BsiWI C'GTAC_G
Cuts at: 1007 1007
Size: 4535
BsmI GAATG_Cn'
Cuts at: 624 1257 624
Size: 633 3902
Bsp1286I G_dGCh'C
Cuts at: 376 1549 2160 2253 2327 2517 2579 3309 376
Size: 1173 611 93 74 190 62 730 1602
Fragments arranged by size:
1602 1173 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3755 3755
Size: 4535
BspHI T'CATG_A
Cuts at: 885 1122 3711 885
Size: 237 2589 1709
Fragments arranged by size:
2589 1709 237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1585 1799 2101 2482 1585
Size: 214 302 381 3638
Fragments arranged by size:
3638 381 302 214
BsrBI CCG'CTC
Cuts at: 50 834 2827 2881 4365 4530 50
Size: 784 1993 54 1484 165 55
Fragments arranged by size:
1993 1484 784 165 55 54
BsrDI GCAATG_nn'
Cuts at: 392 543 804 909 2447 392
Size: 151 261 105 1538 2480
Fragments arranged by size:
2480 1538 261 151 105
BsrFI r'CCGG_y
Cuts at: 2533 2714 2923 2533
Size: 181 209 4145
Fragments arranged by size:
4145 209 181
BssSI C'ACGA_G
Cuts at: 197 2806 3164 197
Size: 2609 358 1568
Fragments arranged by size:
2609 1568 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1912 1912
Size: 4535
BstBI TT'CG_AA
Cuts at: 1241 2896 1241
Size: 1655 2880
BstXI CCAn_nnnn'nTGG
Cuts at: 786 786
Size: 4535
BstYI r'GATC_y
Cuts at: 1 434 1308 1560 2027 2050 2385 2631 3643
Size: 433 874 252 467 23 335 246 1012
Cuts at: 3643 3729 3741 4087 1
Size: 86 12 346 449
Fragments arranged by size:
1012 874 467 449 433 346 335 252
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2485 2666 2829 2485
Size: 181 163 4191
Fragments arranged by size:
4191 181 163
BtsI GCAGTG_nn'
Cuts at: 1349 1778 1349
Size: 429 4106
DraI TTT'AAA
Cuts at: 1442 1534 1442
Size: 92 4443
DrdI GACnn_nn'nnGTC
Cuts at: 418 2241 3099 418
Size: 1823 858 1854
Fragments arranged by size:
1854 1823 858
EaeI y'GGCC_r
Cuts at: 339 788 1403 2120 2294 2685 2712 4057 339
Size: 449 615 717 174 391 27 1345 817
Fragments arranged by size:
1345 817 717 615 449 391 174 27
EagI C'GGCC_G
Cuts at: 2120 2120
Size: 4535
EarI CTCTTCn'nnn_
Cuts at: 887 1226 2558 2768 887
Size: 339 1332 210 2654
Fragments arranged by size:
2654 1332 339 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2743 3053 3199 4345 2743
Size: 310 146 1146 2933
Fragments arranged by size:
2933 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1547 1547
Size: 4535
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1243 2360 2792 3539 3807 1243
Size: 1117 432 747 268 1971
Fragments arranged by size:
1971 1117 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1049 1049
Size: 4535
EcoO109I rG'GnC_Cy
Cuts at: 1674 1674
Size: 4535
EcoRI G'AATT_C
Cuts at: 1539 1539
Size: 4535
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2554 2554
Size: 4535
FspI TGC'GCA
Cuts at: 2316 2316
Size: 4535
HaeII r_GCGC'y
Cuts at: 1969 2217 3239 4218 1969
Size: 248 1022 979 2286
Fragments arranged by size:
2286 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 494 526 1438 1470 2377 2407 2409 2439 494
Size: 32 912 32 907 30 2 30 2590
Fragments arranged by size:
2590 912 907 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1089 1574 1089
Size: 485 4050
HpaI GTT'AAC
Cuts at: 1089 1089
Size: 4535
KasI G'GCGC_C
Cuts at: 1965 2213 1965
Size: 248 4287
KpnI G_GTAC'C
Cuts at: 1555 1555
Size: 4535
MfeI C'AATT_G
Cuts at: 875 875
Size: 4535
MluI A'CGCG_T
Cuts at: 3968 3968
Size: 4535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 550 1409 3206 3390 550
Size: 859 1797 184 1695
Fragments arranged by size:
1797 1695 859 184
MscI TGG'CCA
Cuts at: 341 2296 341
Size: 1955 2580
MslI CAynn'nnrTG
Cuts at: 421 2524 2651 421
Size: 2103 127 2305
Fragments arranged by size:
2305 2103 127
MspA1I CmG'CkG
Cuts at: 401 840 1204 1646 1960 2320 3333 3578 401
Size: 439 364 442 314 360 1013 245 1358
Fragments arranged by size:
1358 1013 442 439 364 360 314 245
NaeI GCC'GGC
Cuts at: 2716 2716
Size: 4535
NarI GG'CG_CC
Cuts at: 1966 2214 1966
Size: 248 4287
NgoMIV G'CCGG_C
Cuts at: 2714 2714
Size: 4535
NsiI A_TGCA'T
Cuts at: 626 626
Size: 4535
NspI r_CATG'y
Cuts at: 145 1068 1588 2088 2995 145
Size: 923 520 500 907 1685
Fragments arranged by size:
1685 923 907 520 500
PciI A'CATG_T
Cuts at: 2991 2991
Size: 4535
PflMI CCAn_nnn'nTGG
Cuts at: 445 445
Size: 4535
PmeI GTTT'AAAC
Cuts at: 1534 1534
Size: 4535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 3674
Size: 32 4503
PsiI TTA'TAA
Cuts at: 607 607
Size: 4535
PstI C_TGCA'G
Cuts at: 1582 2267 1582
Size: 685 3850
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4535
PvuII CAG'CTG
Cuts at: 840 1960 2320 840
Size: 1120 360 3055
Fragments arranged by size:
3055 1120 360
RsrII CG'GwC_CG
Cuts at: 2730 2730
Size: 4535
SacI G_AGCT'C
Cuts at: 1549 1549
Size: 4535
SalI G'TCGA_C
Cuts at: 1572 1572
Size: 4535
SapI GCTCTTCn'nnn_
Cuts at: 887 2558 2768 887
Size: 1671 210 2654
Fragments arranged by size:
2654 1671 210
SbfI CC_TGCA'GG
Cuts at: 1582 1582
Size: 4535
ScaI AGT'ACT
Cuts at: 4052 4052
Size: 4535
SfcI C'TryA_G
Cuts at: 33 329 1578 2263 3256 3447 33
Size: 296 1249 685 993 191 1121
Fragments arranged by size:
1249 1121 993 685 296 191
SfoI GGC'GCC
Cuts at: 1967 2215 1967
Size: 248 4287
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
SmaI CCC'GGG
Cuts at: 1557 1557
Size: 4535
SmlI C'TyrA_G
Cuts at: 1312 1690 2605 3097 3359 1312
Size: 378 915 492 262 2488
Fragments arranged by size:
2488 915 492 378 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4535
SphI G_CATG'C
Cuts at: 1588 2088 1588
Size: 500 4035
StyI C'CwwG_G
Cuts at: 1669 2023 1669
Size: 354 4181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2118 2251 2710 2919 2118
Size: 133 459 209 3734
Fragments arranged by size:
3734 459 209 133
TatI w'GTAC_w
Cuts at: 4050 4050
Size: 4535
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4052 4084 4052
Size: 32 4503
Tth111I GACn'n_nGTC
Cuts at: 2332 2332
Size: 4535
XbaI T'CTAG_A
Cuts at: 1566 1566
Size: 4535
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2030 114
Size: 1916 2619
XmaI C'CCGG_G
Cuts at: 1555 1555
Size: 4535
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BglI BmgBI BmtI
Bpu10I BsaXI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII
NcoI NdeI NheI NotI NruI PacI PasI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI