Restriction map for
pF1KB5633
(Circular) (Six-base) MAPSORT of: pF1KB5633.seq Check: 1434 from: 1 to: 4679
pF1KB5633 4679 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1717 1717
Size: 4679
Acc65I G'GTAC_C
Cuts at: 1695 1695
Size: 4679
AclI AA'CG_TT
Cuts at: 4519 4519
Size: 4679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 1276 1442 2504 2936 3289 3951 664
Size: 612 166 1062 432 353 662 1392
Fragments arranged by size:
1392 1062 662 612 432 353 166
AfeI AGC'GCT
Cuts at: 192 1312 192
Size: 1120 3559
AflIII A'CryG_T
Cuts at: 2031 3831 4112 2031
Size: 1800 281 2598
Fragments arranged by size:
2598 1800 281
AleI CACnn'nnGTG
Cuts at: 1149 1149
Size: 4679
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1167 1300 1167
Size: 133 4546
AlwNI CAG_nnn'CTG
Cuts at: 185 1984 3422 185
Size: 1799 1438 1442
Fragments arranged by size:
1799 1442 1438
ApaLI G'TGCA_C
Cuts at: 3517 3517
Size: 4679
ApoI r'AATT_y
Cuts at: 619 987 1160 1683 4032 4288 619
Size: 368 173 523 2349 256 1010
Fragments arranged by size:
2349 1010 523 368 256 173
AseI AT'TA_AT
Cuts at: 20 629 20
Size: 609 4070
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
AvaI C'yCGr_G
Cuts at: 1261 1699 1261
Size: 438 4241
BamHI G'GATC_C
Cuts at: 139 1704 139
Size: 1565 3114
BanI G'GyrC_C
Cuts at: 1695 2109 2357 2392 1695
Size: 414 248 35 3982
Fragments arranged by size:
3982 414 248 35
BanII G_rGCy'C
Cuts at: 1097 1693 2723 1097
Size: 596 1030 3053
Fragments arranged by size:
3053 1030 596
BbeI G_GCGC'C
Cuts at: 2113 2361 2113
Size: 248 4431
BciVI GTATCCnnnnn_n'
Cuts at: 2577 3633 4679 2577
Size: 1056 1046 2577
Fragments arranged by size:
2577 1056 1046
BclI T'GATC_A
Cuts at: 1413 2199 1413
Size: 786 3893
BfrBI ATG'CAT
Cuts at: 1194 1539 1194
Size: 345 4334
BglII A'GATC_T
Cuts at: 2194 4231 2194
Size: 2037 2642
BlpI GC'TnA_GC
Cuts at: 1307 1791 1307
Size: 484 4195
Bme1580I G_kGCm'C
Cuts at: 2304 2397 3521 2304
Size: 93 1124 3462
Fragments arranged by size:
3462 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 900 1891 1972 2307 4451 900
Size: 991 81 335 2144 1128
Fragments arranged by size:
2144 1128 991 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3046 3046
Size: 4679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 228
Size: 4679
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 1138 1855 2734 3448 3746 571
Size: 567 717 879 714 298 1504
Fragments arranged by size:
1504 879 717 714 567 298
BsaI GGTCTCn'nnnn_
Cuts at: 1608 4383 1608
Size: 2775 1904
BsaAI yAC'GTr
Cuts at: 2032 2032
Size: 4679
BsaBI GATnn'nnATC
Cuts at: 6 2193 4230 6
Size: 2187 2037 455
Fragments arranged by size:
2187 2037 455
BsaHI Gr'CG_yC
Cuts at: 2110 2358 3060 4556 2110
Size: 248 702 1496 2233
Fragments arranged by size:
2233 1496 702 248
BsaWI w'CCGG_w
Cuts at: 983 1868 2389 3067 3478 3625 3899 983
Size: 885 521 678 411 147 274 1763
Fragments arranged by size:
1763 885 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1626 1656 1626
Size: 30 4649
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 274 1228 274
Size: 954 3725
BseYI C'CCAG_C
Cuts at: 2104 2862 3527 3935 2104
Size: 758 665 408 2848
Fragments arranged by size:
2848 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2221 2267 3497 104
Size: 2117 46 1230 1286
Fragments arranged by size:
2117 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1097 1693 2471 2661 3521 1097
Size: 596 778 190 860 2255
Fragments arranged by size:
2255 860 778 596 190
BsmI GAATG_Cn'
Cuts at: 319 1196 319
Size: 877 3802
Bsp1286I G_dGCh'C
Cuts at: 1097 1693 2304 2397 2471 2661 2723 3521 1097
Size: 596 611 93 74 190 62 798 2255
Fragments arranged by size:
2255 798 611 596 190 93 74 62
BspEI T'CCGG_A
Cuts at: 983 983
Size: 4679
BspHI T'CATG_A
Cuts at: 3111 3111
Size: 4679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 400 1729 1943 2245 2626 400
Size: 1329 214 302 381 2453
Fragments arranged by size:
2453 1329 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2971 3025 4509 4674 50
Size: 2921 54 1484 165 55
Fragments arranged by size:
2921 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1161 2591 1161
Size: 1430 3249
BsrFI r'CCGG_y
Cuts at: 2677 2858 2677
Size: 181 4498
BssSI C'ACGA_G
Cuts at: 2950 3658 2950
Size: 708 3971
BstAPI GCAn_nnn'nTGC
Cuts at: 383 2056 383
Size: 1673 3006
BstBI TT'CG_AA
Cuts at: 1110 3040 1110
Size: 1930 2749
BstYI r'GATC_y
Cuts at: 1 139 263 713 1704 2171 2194 2529 2775
Size: 138 124 450 991 467 23 335 246
Cuts at: 2775 3081 3093 3179 4231 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 991 467 450 449 335 306 246
138 124 86 23 12
BtgI C'CryG_G
Cuts at: 173 1501 173
Size: 1328 3351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2629 2810 2973 2629
Size: 181 163 4335
Fragments arranged by size:
4335 181 163
BtsI GCAGTG_nn'
Cuts at: 353 1156 1922 353
Size: 803 766 3110
Fragments arranged by size:
3110 803 766
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1357 1392 1596 1631 1357
Size: 35 204 35 4405
Fragments arranged by size:
4405 204 35 35
DraI TTT'AAA
Cuts at: 1678 1678
Size: 4679
DraIII CAC_nnn'GTG
Cuts at: 571 571
Size: 4679
DrdI GACnn_nn'nnGTC
Cuts at: 2385 3729 2385
Size: 1344 3335
EaeI y'GGCC_r
Cuts at: 170 176 363 2264 2438 2829 2856 4201 170
Size: 6 187 1901 174 391 27 1345 648
Fragments arranged by size:
1901 1345 648 391 187 174 27 6
EagI C'GGCC_G
Cuts at: 2264 2264
Size: 4679
EarI CTCTTCn'nnn_
Cuts at: 1205 1442 2702 2912 1205
Size: 237 1260 210 2972
Fragments arranged by size:
2972 1260 237 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2887 3629 3775 4489 2887
Size: 742 146 714 3077
Fragments arranged by size:
3077 742 714 146
EcoICRI GAG'CTC
Cuts at: 1095 1691 1095
Size: 596 4083
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 664 1276 1442 2504 2936 3289 3951 228
Size: 436 612 166 1062 432 353 662 956
Fragments arranged by size:
1062 956 662 612 436 432 353 166
EcoO109I rG'GnC_Cy
Cuts at: 1818 1818
Size: 4679
EcoRI G'AATT_C
Cuts at: 1683 1683
Size: 4679
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 4679
FspI TGC'GCA
Cuts at: 2460 2460
Size: 4679
HaeII r_GCGC'y
Cuts at: 194 1314 2113 2361 3591 4362 194
Size: 1120 799 248 1230 771 511
Fragments arranged by size:
1230 1120 799 771 511 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 477 509 777 809 1481 1513 2521 2551 2553
Size: 32 268 32 672 32 1008 30 2
Cuts at: 2553 2583 477
Size: 30 2573
Fragments arranged by size:
2573 1008 672 268 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 429 1718 429
Size: 1289 3390
KasI G'GCGC_C
Cuts at: 2109 2357 2109
Size: 248 4431
KpnI G_GTAC'C
Cuts at: 1699 1699
Size: 4679
MluI A'CGCG_T
Cuts at: 4112 4112
Size: 4679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3438 3622 3438
Size: 184 4495
MscI TGG'CCA
Cuts at: 172 178 2440 172
Size: 6 2262 2411
Fragments arranged by size:
2411 2262 6
MslI CAynn'nnrTG
Cuts at: 919 1149 2668 2795 919
Size: 230 1519 127 2803
Fragments arranged by size:
2803 1519 230 127
MspA1I CmG'CkG
Cuts at: 134 1790 2104 2464 3248 3493 134
Size: 1656 314 360 784 245 1320
Fragments arranged by size:
1656 1320 784 360 314 245
NaeI GCC'GGC
Cuts at: 2860 2860
Size: 4679
NarI GG'CG_CC
Cuts at: 2110 2358 2110
Size: 248 4431
NcoI C'CATG_G
Cuts at: 173 173
Size: 4679
NgoMIV G'CCGG_C
Cuts at: 2858 2858
Size: 4679
NsiI A_TGCA'T
Cuts at: 1196 1541 1196
Size: 345 4334
NspI r_CATG'y
Cuts at: 1281 1732 2232 3835 1281
Size: 451 500 1603 2125
Fragments arranged by size:
2125 1603 500 451
PciI A'CATG_T
Cuts at: 3831 3831
Size: 4679
PfoI T'CCnGG_A
Cuts at: 992 1046 992
Size: 54 4625
PmeI GTTT'AAAC
Cuts at: 1678 1678
Size: 4679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3125 3157 3125
Size: 32 4647
PstI C_TGCA'G
Cuts at: 1726 2411 1726
Size: 685 3994
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4679
PvuII CAG'CTG
Cuts at: 134 2104 2464 134
Size: 1970 360 2349
Fragments arranged by size:
2349 1970 360
RsrII CG'GwC_CG
Cuts at: 2874 2874
Size: 4679
SacI G_AGCT'C
Cuts at: 1097 1693 1097
Size: 596 4083
SalI G'TCGA_C
Cuts at: 1716 1716
Size: 4679
SapI GCTCTTCn'nnn_
Cuts at: 2702 2912 2702
Size: 210 4469
SbfI CC_TGCA'GG
Cuts at: 1726 1726
Size: 4679
ScaI AGT'ACT
Cuts at: 843 1659 4196 843
Size: 816 2537 1326
Fragments arranged by size:
2537 1326 816
SfcI C'TryA_G
Cuts at: 33 186 815 1722 2407 3375 3566 33
Size: 153 629 907 685 968 191 1146
Fragments arranged by size:
1146 968 907 685 629 191 153
SfoI GGC'GCC
Cuts at: 2111 2359 2111
Size: 248 4431
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
SmaI CCC'GGG
Cuts at: 1263 1701 1263
Size: 438 4241
SmlI C'TyrA_G
Cuts at: 550 1117 1834 2749 3463 3725 550
Size: 567 717 915 714 262 1504
Fragments arranged by size:
1504 915 717 714 567 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4679
SphI G_CATG'C
Cuts at: 1281 1732 2232 1281
Size: 451 500 3728
Fragments arranged by size:
3728 500 451
SspI AAT'ATT
Cuts at: 633 633
Size: 4679
StyI C'CwwG_G
Cuts at: 173 655 1375 1813 2167 173
Size: 482 720 438 354 2685
Fragments arranged by size:
2685 720 482 438 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 205 919 2262 2395 2854 3063 205
Size: 714 1343 133 459 209 1821
Fragments arranged by size:
1821 1343 714 459 209 133
TatI w'GTAC_w
Cuts at: 841 1657 4194 841
Size: 816 2537 1326
Fragments arranged by size:
2537 1326 816
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4196 4228 4196
Size: 32 4647
Tth111I GACn'n_nGTC
Cuts at: 2476 2476
Size: 4679
XbaI T'CTAG_A
Cuts at: 804 1710 804
Size: 906 3773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 252 327 2174 252
Size: 75 1847 2757
Fragments arranged by size:
2757 1847 75
XmaI C'CCGG_G
Cuts at: 1261 1699 1261
Size: 438 4241
XmnI GAAnn'nnTTC
Cuts at: 1001 1001
Size: 4679
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI