Restriction map for
pF1KB5625
(Circular) (Six-base) MAPSORT of: pF1KB5625.seq Check: 2622 from: 1 to: 6872
pF1KB5625 6872 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 590 3059 3910 590
Size: 2469 851 3552
Fragments arranged by size:
3552 2469 851
Acc65I G'GTAC_C
Cuts at: 3888 3888
Size: 6872
AclI AA'CG_TT
Cuts at: 3594 6712 3594
Size: 3118 3754
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1085 2137 2251 4697 5129 5876 6144 1085
Size: 1052 114 2446 432 747 268 1813
Fragments arranged by size:
2446 1813 1052 747 432 268 114
AflII C'TTAA_G
Cuts at: 1918 1918
Size: 6872
AflIII A'CryG_T
Cuts at: 4224 5328 6305 4224
Size: 1104 977 4791
Fragments arranged by size:
4791 1104 977
AgeI A'CCGG_T
Cuts at: 5260 5260
Size: 6872
AleI CACnn'nnGTG
Cuts at: 2707 2707
Size: 6872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3260 3260
Size: 6872
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 639 671 639
Size: 32 6840
AlwNI CAG_nnn'CTG
Cuts at: 1455 1475 2349 3046 4177 5744 1455
Size: 20 874 697 1131 1567 2583
Fragments arranged by size:
2583 1567 1131 874 697 20
ApaLI G'TGCA_C
Cuts at: 5642 5642
Size: 6872
ApoI r'AATT_y
Cuts at: 1273 2399 3025 3876 6225 6481 1273
Size: 1126 626 851 2349 256 1664
Fragments arranged by size:
2349 1664 1126 851 626 256
AseI AT'TA_AT
Cuts at: 20 2618 20
Size: 2598 4274
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6872
AvaI C'yCGr_G
Cuts at: 1197 1909 3892 1197
Size: 712 1983 4177
Fragments arranged by size:
4177 1983 712
AvrII C'CTAG_G
Cuts at: 2892 2892
Size: 6872
BamHI G'GATC_C
Cuts at: 3897 3897
Size: 6872
BanI G'GyrC_C
Cuts at: 1628 3888 4302 4550 4585 1628
Size: 2260 414 248 35 3915
Fragments arranged by size:
3915 2260 414 248 35
BanII G_rGCy'C
Cuts at: 1033 1612 3306 3886 4916 1033
Size: 579 1694 580 1030 2989
Fragments arranged by size:
2989 1694 1030 580 579
BbeI G_GCGC'C
Cuts at: 4306 4554 4306
Size: 248 6624
BbsI GAAGACnn'nnnn_
Cuts at: 173 2182 2282 3467 3544 173
Size: 2009 100 1185 77 3501
Fragments arranged by size:
3501 2009 1185 100 77
BbvCI CC'TCA_GC
Cuts at: 220 220
Size: 6872
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 611 645 611
Size: 34 6838
BciVI GTATCCnnnnn_n'
Cuts at: 1825 4770 5531 6872 1825
Size: 2945 761 1341 1825
Fragments arranged by size:
2945 1825 1341 761
BclI T'GATC_A
Cuts at: 492 1170 1944 3705 4392 492
Size: 678 774 1761 687 2972
Fragments arranged by size:
2972 1761 774 687 678
BglII A'GATC_T
Cuts at: 1855 4387 6424 1855
Size: 2532 2037 2303
Fragments arranged by size:
2532 2303 2037
BlpI GC'TnA_GC
Cuts at: 3984 3984
Size: 6872
Bme1580I G_kGCm'C
Cuts at: 3420 4497 4590 5646 3420
Size: 1077 93 1056 4646
Fragments arranged by size:
4646 1077 1056 93
BmgBI CAC'GTC
Cuts at: 1372 1372
Size: 6872
BmrI ACTGGGnnnn_n'
Cuts at: 1523 4084 4165 4500 6644 1523
Size: 2561 81 335 2144 1751
Fragments arranged by size:
2561 2144 1751 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 186 2181 3571 5239 186
Size: 1995 1390 1668 1819
Fragments arranged by size:
1995 1819 1668 1390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 816 1238 1477 2302 2526 816
Size: 422 239 825 224 5162
Fragments arranged by size:
5162 825 422 239 224
Bpu10I CC'TnA_GC
Cuts at: 220 360 1032 220
Size: 140 672 6060
Fragments arranged by size:
6060 672 140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 546 549 1576 3697 4048 4927 5419 5717 546
Size: 3 1027 2121 351 879 492 298 1701
Fragments arranged by size:
2121 1701 1027 879 492 351 298 3
BsaI GGTCTCn'nnnn_
Cuts at: 1453 3139 6576 1453
Size: 1686 3437 1749
Fragments arranged by size:
3437 1749 1686
BsaAI yAC'GTr
Cuts at: 4225 4225
Size: 6872
BsaBI GATnn'nnATC
Cuts at: 6 4386 6423 6
Size: 4380 2037 455
Fragments arranged by size:
4380 2037 455
BsaHI Gr'CG_yC
Cuts at: 3324 4303 4551 5253 6749 3324
Size: 979 248 702 1496 3447
Fragments arranged by size:
3447 1496 979 702 248
BsaWI w'CCGG_w
Cuts at: 1634 2060 3452 4061 4582 5260 5534 5681 6092
Size: 426 1392 609 521 678 274 147 411
Cuts at: 6092 1634
Size: 2414
Fragments arranged by size:
2414 1392 678 609 521 426 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1573 1603 1668 1698 1740 1770 1573
Size: 30 65 30 42 30 6675
Fragments arranged by size:
6675 65 42 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1137 1594 1748 1137
Size: 457 154 6261
Fragments arranged by size:
6261 457 154
BseYI C'CCAG_C
Cuts at: 1322 2180 4297 5055 5632 6128 1322
Size: 858 2117 758 577 496 2066
Fragments arranged by size:
2117 2066 858 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1555 1555
Size: 6872
BsiEI CG_ry'CG
Cuts at: 104 4414 4460 5668 104
Size: 4310 46 1208 1308
Fragments arranged by size:
4310 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1033 1720 1989 2741 2924 3886 4664 4854 5646
Size: 687 269 752 183 962 778 190 792
Cuts at: 5646 1033
Size: 2259
Fragments arranged by size:
2259 962 792 778 752 687 269 190
183
BsmI GAATG_Cn'
Cuts at: 180 743 3723 180
Size: 563 2980 3329
Fragments arranged by size:
3329 2980 563
Bsp1286I G_dGCh'C
Cuts at: 1033 1612 1720 1989 2741 2924 3306 3420 3886
Size: 579 108 269 752 183 382 114 466
Cuts at: 3886 4497 4590 4664 4854 4916 5646 1033
Size: 611 93 74 190 62 730 2259
Fragments arranged by size:
2259 752 730 611 579 466 382 269
190 183 114 108 93 74 62
BspEI T'CCGG_A
Cuts at: 3452 6092 3452
Size: 2640 4232
BspHI T'CATG_A
Cuts at: 1604 3765 6048 1604
Size: 2161 2283 2428
Fragments arranged by size:
2428 2283 2161
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3452 3550 3922 4136 4438 4819 3452
Size: 98 372 214 302 381 5505
Fragments arranged by size:
5505 381 372 302 214 98
BsrBI CCG'CTC
Cuts at: 50 5164 5218 6702 6867 50
Size: 5114 54 1484 165 55
Fragments arranged by size:
5114 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1880 4784 1880
Size: 2904 3968
BsrFI r'CCGG_y
Cuts at: 1394 4870 5051 5260 1394
Size: 3476 181 209 3006
Fragments arranged by size:
3476 3006 209 181
BsrGI T'GTAC_A
Cuts at: 884 884
Size: 6872
BssSI C'ACGA_G
Cuts at: 1112 5143 5501 1112
Size: 4031 358 2483
Fragments arranged by size:
4031 2483 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4249 4249
Size: 6872
BstBI TT'CG_AA
Cuts at: 5233 5233
Size: 6872
BstYI r'GATC_y
Cuts at: 1 975 1264 1855 3812 3897 4364 4387 4722
Size: 974 289 591 1957 85 467 23 335
Cuts at: 4722 4968 5980 6066 6078 6424 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1957 1012 974 591 467 449 346 335
289 246 86 85 23 12
Bsu36I CC'TnA_GG
Cuts at: 1311 1311
Size: 6872
BtgI C'CryG_G
Cuts at: 107 1523 2942 107
Size: 1416 1419 4037
Fragments arranged by size:
4037 1419 1416
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3779 4822 5003 5166 3779
Size: 1043 181 163 5485
Fragments arranged by size:
5485 1043 181 163
BtsI GCAGTG_nn'
Cuts at: 1698 2853 4115 1698
Size: 1155 1262 4455
Fragments arranged by size:
4455 1262 1155
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3549 3584 3549
Size: 35 6837
DraI TTT'AAA
Cuts at: 255 2588 3871 255
Size: 2333 1283 3256
Fragments arranged by size:
3256 2333 1283
DrdI GACnn_nn'nnGTC
Cuts at: 4578 5436 4578
Size: 858 6014
EaeI y'GGCC_r
Cuts at: 504 638 2945 3495 4457 4631 5022 5049 6394
Size: 134 2307 550 962 174 391 27 1345
Cuts at: 6394 504
Size: 982
Fragments arranged by size:
2307 1345 982 962 550 391 174 134
27
EagI C'GGCC_G
Cuts at: 4457 4457
Size: 6872
EarI CTCTTCn'nnn_
Cuts at: 107 243 474 2227 4895 5105 107
Size: 136 231 1753 2668 210 1874
Fragments arranged by size:
2668 1874 1753 231 210 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5080 5390 5536 6682 5080
Size: 310 146 1146 5270
Fragments arranged by size:
5270 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1031 3884 1031
Size: 2853 4019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 816 1085 1238 1477 2137 2251 2302 2526 4697
Size: 269 153 239 660 114 51 224 2171
Cuts at: 4697 5129 5876 6144 816
Size: 432 747 268 1544
Fragments arranged by size:
2171 1544 747 660 432 269 268 239
224 153 114 51
EcoNI CCTnn'n_nnAGG
Cuts at: 1303 1303
Size: 6872
EcoO109I rG'GnC_Cy
Cuts at: 521 4011 521
Size: 3490 3382
EcoRI G'AATT_C
Cuts at: 3876 3876
Size: 6872
EcoRV GAT'ATC
Cuts at: 469 469
Size: 6872
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 728 2349 3480 4891 728
Size: 1621 1131 1411 2709
Fragments arranged by size:
2709 1621 1411 1131
FspI TGC'GCA
Cuts at: 4653 4653
Size: 6872
HaeII r_GCGC'y
Cuts at: 763 4306 4554 5576 6555 763
Size: 3543 248 1022 979 1080
Fragments arranged by size:
3543 1080 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 781 813 1374 1406 1437 1469 1567 1599 1668
Size: 32 561 32 31 32 98 32 69
Cuts at: 1668 1700 2034 2066 2944 2976 3163 3195 3542
Size: 32 334 32 878 32 187 32 347
Cuts at: 3542 3574 3720 3752 4714 4744 4746 4776 781
Size: 32 146 32 962 30 2 30 2877
Fragments arranged by size:
2877 962 878 561 347 334 187 146
98 69 32 32 32 32 32 32
32 32 32 32 31 30 30 2
HincII GTy'rAC
Cuts at: 776 2574 3911 776
Size: 1798 1337 3737
Fragments arranged by size:
3737 1798 1337
HindIII A'AGCT_T
Cuts at: 1787 2119 1787
Size: 332 6540
HpaI GTT'AAC
Cuts at: 776 2574 776
Size: 1798 5074
KasI G'GCGC_C
Cuts at: 4302 4550 4302
Size: 248 6624
KpnI G_GTAC'C
Cuts at: 3892 3892
Size: 6872
MfeI C'AATT_G
Cuts at: 2190 2190
Size: 6872
MluI A'CGCG_T
Cuts at: 6305 6305
Size: 6872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 454 595 1952 2231 5543 5727 454
Size: 141 1357 279 3312 184 1599
Fragments arranged by size:
3312 1599 1357 279 184 141
MscI TGG'CCA
Cuts at: 640 2947 3497 4633 640
Size: 2307 550 1136 2879
Fragments arranged by size:
2879 2307 1136 550
MslI CAynn'nnrTG
Cuts at: 584 2707 3562 4861 4988 584
Size: 2123 855 1299 127 2468
Fragments arranged by size:
2468 2123 1299 855 127
MspA1I CmG'CkG
Cuts at: 906 2184 2707 2909 3983 4297 4657 5670 5915
Size: 1278 523 202 1074 314 360 1013 245
Cuts at: 5915 906
Size: 1863
Fragments arranged by size:
1863 1278 1074 1013 523 360 314 245
202
NaeI GCC'GGC
Cuts at: 5053 5053
Size: 6872
NarI GG'CG_CC
Cuts at: 4303 4551 4303
Size: 248 6624
NcoI C'CATG_G
Cuts at: 107 2942 107
Size: 2835 4037
NdeI CA'TA_TG
Cuts at: 1182 1182
Size: 6872
NgoMIV G'CCGG_C
Cuts at: 5051 5051
Size: 6872
NspI r_CATG'y
Cuts at: 711 3925 4425 5332 711
Size: 3214 500 907 2251
Fragments arranged by size:
3214 2251 907 500
PciI A'CATG_T
Cuts at: 5328 5328
Size: 6872
PfoI T'CCnGG_A
Cuts at: 3335 3335
Size: 6872
PmeI GTTT'AAAC
Cuts at: 3871 3871
Size: 6872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6011 6043 6011
Size: 32 6840
PshAI GACnn'nnGTC
Cuts at: 2192 2192
Size: 6872
PsiI TTA'TAA
Cuts at: 2984 3032 2984
Size: 48 6824
PspXI vC'TCGA_Gb
Cuts at: 1909 1909
Size: 6872
PstI C_TGCA'G
Cuts at: 1568 3919 4604 1568
Size: 2351 685 3836
Fragments arranged by size:
3836 2351 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6872
PvuII CAG'CTG
Cuts at: 906 2184 2707 2909 4297 4657 906
Size: 1278 523 202 1388 360 3121
Fragments arranged by size:
3121 1388 1278 523 360 202
RsrII CG'GwC_CG
Cuts at: 5067 5067
Size: 6872
SacI G_AGCT'C
Cuts at: 1033 3886 1033
Size: 2853 4019
SalI G'TCGA_C
Cuts at: 3909 3909
Size: 6872
SapI GCTCTTCn'nnn_
Cuts at: 107 474 4895 5105 107
Size: 367 4421 210 1874
Fragments arranged by size:
4421 1874 367 210
SbfI CC_TGCA'GG
Cuts at: 3919 3919
Size: 6872
ScaI AGT'ACT
Cuts at: 829 6389 829
Size: 5560 1312
SfcI C'TryA_G
Cuts at: 33 861 1564 3915 4600 5593 5784 33
Size: 828 703 2351 685 993 191 1121
Fragments arranged by size:
2351 1121 993 828 703 685 191
SfoI GGC'GCC
Cuts at: 4304 4552 4304
Size: 248 6624
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6872
SgrAI Cr'CCGG_yG
Cuts at: 1394 1394
Size: 6872
SmaI CCC'GGG
Cuts at: 3894 3894
Size: 6872
SmlI C'TyrA_G
Cuts at: 525 564 1555 1909 1918 3712 4027 4942 5434
Size: 39 991 354 9 1794 315 915 492
Cuts at: 5434 5696 525
Size: 262 1701
Fragments arranged by size:
1794 1701 991 915 492 354 315 262
39 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6872
SphI G_CATG'C
Cuts at: 711 3925 4425 711
Size: 3214 500 3158
Fragments arranged by size:
3214 3158 500
SspI AAT'ATT
Cuts at: 414 3201 3504 414
Size: 2787 303 3782
Fragments arranged by size:
3782 2787 303
StuI AGG'CCT
Cuts at: 1309 2783 3106 1309
Size: 1474 323 5075
Fragments arranged by size:
5075 1474 323
StyI C'CwwG_G
Cuts at: 107 2105 2892 2942 4006 4360 107
Size: 1998 787 50 1064 354 2619
Fragments arranged by size:
2619 1998 1064 787 354 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3556 4455 4588 5047 5256 3556
Size: 899 133 459 209 5172
Fragments arranged by size:
5172 899 459 209 133
TatI w'GTAC_w
Cuts at: 827 884 6387 827
Size: 57 5503 1312
Fragments arranged by size:
5503 1312 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1740 1772 2649 2681 6389 6421 1740
Size: 32 877 32 3708 32 2191
Fragments arranged by size:
3708 2191 877 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 586 1451 4669 586
Size: 865 3218 2789
Fragments arranged by size:
3218 2789 865
XbaI T'CTAG_A
Cuts at: 2313 2386 3903 2313
Size: 73 1517 5282
Fragments arranged by size:
5282 1517 73
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1113 2709 4367 1113
Size: 1596 1658 3618
Fragments arranged by size:
3618 1658 1596
XhoI C'TCGA_G
Cuts at: 1909 1909
Size: 6872
XmaI C'CCGG_G
Cuts at: 3892 3892
Size: 6872
XmnI GAAnn'nnTTC
Cuts at: 321 3070 321
Size: 2749 4123
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PfoI PmeI PpiI PshAI PsiI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI ApaI AscI BaeI BfrBI
BglI BmtI BsiWI BsmBI BssHII BstEII BstXI BstZ17I
ClaI DraIII FseI FspAI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PspOMI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI SwaI ZraI