Restriction map for
pF1KB5607
(Circular) (Six-base) MAPSORT of: pF1KB5607.seq Check: 2458 from: 1 to: 4508
pF1KB5607 4508 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 517 943 517
Size: 426 4082
AccI GT'mk_AC
Cuts at: 638 716 1546 638
Size: 78 830 3600
Fragments arranged by size:
3600 830 78
Acc65I G'GTAC_C
Cuts at: 754 1524 754
Size: 770 3738
AclI AA'CG_TT
Cuts at: 4348 4348
Size: 4508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 2765 3512 3780 2333
Size: 432 747 268 3061
Fragments arranged by size:
3061 747 432 268
AflIII A'CryG_T
Cuts at: 361 1860 2964 3941 361
Size: 1499 1104 977 928
Fragments arranged by size:
1499 1104 977 928
AgeI A'CCGG_T
Cuts at: 2896 2896
Size: 4508
AhdI GACnn_n'nnGTC
Cuts at: 1475 1475
Size: 4508
AleI CACnn'nnGTG
Cuts at: 674 766 674
Size: 92 4416
AlwNI CAG_nnn'CTG
Cuts at: 193 1102 1156 1813 3380 193
Size: 909 54 657 1567 1321
Fragments arranged by size:
1567 1321 909 657 54
ApaI G_GGCC'C
Cuts at: 784 784
Size: 4508
ApaLI G'TGCA_C
Cuts at: 3278 3278
Size: 4508
ApoI r'AATT_y
Cuts at: 1512 3861 4117 1512
Size: 2349 256 1903
Fragments arranged by size:
2349 1903 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4508
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
AvaI C'yCGr_G
Cuts at: 710 1016 1417 1528 710
Size: 306 401 111 3690
Fragments arranged by size:
3690 401 306 111
BamHI G'GATC_C
Cuts at: 1533 1533
Size: 4508
BanI G'GyrC_C
Cuts at: 754 961 1524 1938 2186 2221 754
Size: 207 563 414 248 35 3041
Fragments arranged by size:
3041 563 414 248 207 35
BanII G_rGCy'C
Cuts at: 138 374 784 1322 1522 2552 138
Size: 236 410 538 200 1030 2094
Fragments arranged by size:
2094 1030 538 410 236 200
BbeI G_GCGC'C
Cuts at: 1942 2190 1942
Size: 248 4260
BbvCI CC'TCA_GC
Cuts at: 942 942
Size: 4508
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 805 839 805
Size: 34 4474
BciVI GTATCCnnnnn_n'
Cuts at: 2406 3167 4508 2406
Size: 761 1341 2406
Fragments arranged by size:
2406 1341 761
BclI T'GATC_A
Cuts at: 2028 2028
Size: 4508
BglII A'GATC_T
Cuts at: 1187 2023 4060 1187
Size: 836 2037 1635
Fragments arranged by size:
2037 1635 836
BlpI GC'TnA_GC
Cuts at: 1620 1620
Size: 4508
Bme1580I G_kGCm'C
Cuts at: 361 784 2133 2226 3282 361
Size: 423 1349 93 1056 1587
Fragments arranged by size:
1587 1349 1056 423 93
BmrI ACTGGGnnnn_n'
Cuts at: 729 1720 1801 2136 4280 729
Size: 991 81 335 2144 957
Fragments arranged by size:
2144 991 957 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 154 810 2875 154
Size: 656 2065 1787
Fragments arranged by size:
2065 1787 656
Bpu10I CC'TnA_GC
Cuts at: 785 942 1321 785
Size: 157 379 3972
Fragments arranged by size:
3972 379 157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1684 2563 3055 3353 1684
Size: 879 492 298 2839
Fragments arranged by size:
2839 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1111 1385 4212 1111
Size: 274 2827 1407
Fragments arranged by size:
2827 1407 274
BsaAI yAC'GTr
Cuts at: 676 1861 676
Size: 1185 3323
BsaBI GATnn'nnATC
Cuts at: 6 2022 4059 6
Size: 2016 2037 455
Fragments arranged by size:
2037 2016 455
BsaHI Gr'CG_yC
Cuts at: 1939 2187 2889 4385 1939
Size: 248 702 1496 2062
Fragments arranged by size:
2062 1496 702 248
BsaWI w'CCGG_w
Cuts at: 204 1697 2218 2896 3170 3317 3728 204
Size: 1493 521 678 274 147 411 984
Fragments arranged by size:
1493 984 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 600 630 803 833 1121 1151 600
Size: 30 173 30 288 30 3957
Fragments arranged by size:
3957 288 173 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 146 250 1129 1330 146
Size: 104 879 201 3324
Fragments arranged by size:
3324 879 201 104
BseYI C'CCAG_C
Cuts at: 1472 1933 2691 3268 3764 1472
Size: 461 758 577 496 2216
Fragments arranged by size:
2216 758 577 496 461
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 495 921 495
Size: 426 4082
BsiEI CG_ry'CG
Cuts at: 104 2050 2096 3304 104
Size: 1946 46 1208 1308
Fragments arranged by size:
1946 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 138 374 1068 1322 1522 2300 2490 3282 138
Size: 236 694 254 200 778 190 792 1364
Fragments arranged by size:
1364 792 778 694 254 236 200 190
BsmBI CGTCTCn'nnnn_
Cuts at: 885 885
Size: 4508
Bsp1286I G_dGCh'C
Cuts at: 138 361 374 784 1068 1322 1522 2133 2226
Size: 223 13 410 284 254 200 611 93
Cuts at: 2226 2300 2490 2552 3282 138
Size: 74 190 62 730 1364
Fragments arranged by size:
1364 730 611 410 284 254 223 200
190 93 74 62 13
BspEI T'CCGG_A
Cuts at: 204 3728 204
Size: 3524 984
BspHI T'CATG_A
Cuts at: 3684 3684
Size: 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 178 517 943 1558 1772 2074 2455 178
Size: 339 426 615 214 302 381 2231
Fragments arranged by size:
2231 615 426 381 339 302 214
BsrBI CCG'CTC
Cuts at: 50 2800 2854 4338 4503 50
Size: 2750 54 1484 165 55
Fragments arranged by size:
2750 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 424 2420 424
Size: 1996 2512
BsrFI r'CCGG_y
Cuts at: 2506 2687 2896 2506
Size: 181 209 4118
Fragments arranged by size:
4118 209 181
BssSI C'ACGA_G
Cuts at: 2779 3137 2779
Size: 358 4150
BstAPI GCAn_nnn'nTGC
Cuts at: 423 1885 423
Size: 1462 3046
BstBI TT'CG_AA
Cuts at: 2869 2869
Size: 4508
BstXI CCAn_nnnn'nTGG
Cuts at: 985 985
Size: 4508
BstYI r'GATC_y
Cuts at: 1 645 656 1187 1533 2000 2023 2358 2604
Size: 644 11 531 346 467 23 335 246
Cuts at: 2604 3616 3702 3714 4060 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 644 531 467 449 346 346 335
246 86 23 12 11
BstZ17I GTA'TAC
Cuts at: 717 717
Size: 4508
Bsu36I CC'TnA_GG
Cuts at: 1265 1265
Size: 4508
BtgI C'CryG_G
Cuts at: 107 107
Size: 4508
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2458 2639 2802 2458
Size: 181 163 4164
Fragments arranged by size:
4164 181 163
BtsI GCAGTG_nn'
Cuts at: 173 830 1751 173
Size: 657 921 2930
Fragments arranged by size:
2930 921 657
DraI TTT'AAA
Cuts at: 1507 1507
Size: 4508
DrdI GACnn_nn'nnGTC
Cuts at: 268 2214 3072 268
Size: 1946 858 1704
Fragments arranged by size:
1946 1704 858
EaeI y'GGCC_r
Cuts at: 110 2093 2267 2658 2685 4030 110
Size: 1983 174 391 27 1345 588
Fragments arranged by size:
1983 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 2093 2093
Size: 4508
EarI CTCTTCn'nnn_
Cuts at: 2531 2741 2531
Size: 210 4298
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2716 3026 3172 4318 2716
Size: 310 146 1146 2906
Fragments arranged by size:
2906 1146 310 146
EcoICRI GAG'CTC
Cuts at: 136 372 1320 1520 136
Size: 236 948 200 3124
Fragments arranged by size:
3124 948 236 200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 2765 3512 3780 2333
Size: 432 747 268 3061
Fragments arranged by size:
3061 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 456 456
Size: 4508
EcoO109I rG'GnC_Cy
Cuts at: 240 723 780 781 1647 240
Size: 483 57 1 866 3101
Fragments arranged by size:
3101 866 483 57 1
EcoRI G'AATT_C
Cuts at: 1512 1512
Size: 4508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 4508
FspI TGC'GCA
Cuts at: 2289 2289
Size: 4508
HaeII r_GCGC'y
Cuts at: 1338 1942 2190 3212 4191 1338
Size: 604 248 1022 979 1655
Fragments arranged by size:
1655 1022 979 604 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 648 680 1127 1159 1461 1493 2350 2380 2382
Size: 32 447 32 302 32 857 30 2
Cuts at: 2382 2412 648
Size: 30 2744
Fragments arranged by size:
2744 857 447 302 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1086 1092 1469 1547 1086
Size: 6 377 78 4047
Fragments arranged by size:
4047 377 78 6
KasI G'GCGC_C
Cuts at: 1938 2186 1938
Size: 248 4260
KpnI G_GTAC'C
Cuts at: 758 1528 758
Size: 770 3738
MluI A'CGCG_T
Cuts at: 361 3941 361
Size: 3580 928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1190 3179 3363 1190
Size: 1989 184 2335
Fragments arranged by size:
2335 1989 184
MscI TGG'CCA
Cuts at: 112 2269 112
Size: 2157 2351
MslI CAynn'nnrTG
Cuts at: 177 674 766 983 2497 2624 177
Size: 497 92 217 1514 127 2061
Fragments arranged by size:
2061 1514 497 217 127 92
MspA1I CmG'CkG
Cuts at: 551 662 863 946 1099 1296 1619 1933 2293
Size: 111 201 83 153 197 323 314 360
Cuts at: 2293 3306 3551 551
Size: 1013 245 1508
Fragments arranged by size:
1508 1013 360 323 314 245 201 197
153 111 83
NaeI GCC'GGC
Cuts at: 2689 2689
Size: 4508
NarI GG'CG_CC
Cuts at: 1939 2187 1939
Size: 248 4260
NcoI C'CATG_G
Cuts at: 107 107
Size: 4508
NdeI CA'TA_TG
Cuts at: 1495 1495
Size: 4508
NgoMIV G'CCGG_C
Cuts at: 2687 2687
Size: 4508
NspI r_CATG'y
Cuts at: 320 820 1234 1561 2061 2968 320
Size: 500 414 327 500 907 1860
Fragments arranged by size:
1860 907 500 500 414 327
PciI A'CATG_T
Cuts at: 2964 2964
Size: 4508
PfoI T'CCnGG_A
Cuts at: 299 1283 299
Size: 984 3524
PmeI GTTT'AAAC
Cuts at: 1507 1507
Size: 4508
PmlI CAC'GTG
Cuts at: 676 676
Size: 4508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 3647
Size: 32 4476
PpuMI rG'GwC_Cy
Cuts at: 240 723 240
Size: 483 4025
PsiI TTA'TAA
Cuts at: 1178 1178
Size: 4508
PspOMI G'GGCC_C
Cuts at: 780 780
Size: 4508
PspXI vC'TCGA_Gb
Cuts at: 710 710
Size: 4508
PstI C_TGCA'G
Cuts at: 460 1555 2240 460
Size: 1095 685 2728
Fragments arranged by size:
2728 1095 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4508
PvuII CAG'CTG
Cuts at: 551 863 1099 1296 1933 2293 551
Size: 312 236 197 637 360 2766
Fragments arranged by size:
2766 637 360 312 236 197
RsrII CG'GwC_CG
Cuts at: 2703 2703
Size: 4508
SacI G_AGCT'C
Cuts at: 138 374 1322 1522 138
Size: 236 948 200 3124
Fragments arranged by size:
3124 948 236 200
SalI G'TCGA_C
Cuts at: 1545 1545
Size: 4508
SanDI GG'GwC_CC
Cuts at: 723 723
Size: 4508
SapI GCTCTTCn'nnn_
Cuts at: 2531 2741 2531
Size: 210 4298
SbfI CC_TGCA'GG
Cuts at: 1555 1555
Size: 4508
ScaI AGT'ACT
Cuts at: 4025 4025
Size: 4508
SfcI C'TryA_G
Cuts at: 33 456 546 1198 1551 2236 3229 3420 33
Size: 423 90 652 353 685 993 191 1121
Fragments arranged by size:
1121 993 685 652 423 353 191 90
SfoI GGC'GCC
Cuts at: 1940 2188 1940
Size: 248 4260
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
SmaI CCC'GGG
Cuts at: 1018 1530 1018
Size: 512 3996
SmlI C'TyrA_G
Cuts at: 710 1663 2578 3070 3332 710
Size: 953 915 492 262 1886
Fragments arranged by size:
1886 953 915 492 262
SpeI A'CTAG_T
Cuts at: 67 493 67
Size: 426 4082
SphI G_CATG'C
Cuts at: 1561 2061 1561
Size: 500 4008
StyI C'CwwG_G
Cuts at: 107 1642 1996 107
Size: 1535 354 2619
Fragments arranged by size:
2619 1535 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 857 1403 2091 2224 2683 2892 857
Size: 546 688 133 459 209 2473
Fragments arranged by size:
2473 688 546 459 209 133
TatI w'GTAC_w
Cuts at: 882 4023 882
Size: 3141 1367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 600 632 803 835 4025 4057 600
Size: 32 171 32 3190 32 1051
Fragments arranged by size:
3190 1051 171 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2305 2305
Size: 4508
XbaI T'CTAG_A
Cuts at: 1539 1539
Size: 4508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 434 1215 1245 2003 434
Size: 781 30 758 2939
Fragments arranged by size:
2939 781 758 30
XhoI C'TCGA_G
Cuts at: 710 710
Size: 4508
XmaI C'CCGG_G
Cuts at: 1016 1528 1016
Size: 512 3996
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BbsI BfrBI BglI BmgBI BmtI BpmI BsiWI BsmI
BsrGI BssHII BstEII ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PasI PflMI PshAI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI