Restriction map for
pF1KB5580
(Circular) (Six-base) MAPSORT of: pF1KB5580.seq Check: 7376 from: 1 to: 4958
pF1KB5580 4958 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1523 1523
Size: 4958
AatII G_ACGT'C
Cuts at: 488 488
Size: 4958
AccI GT'mk_AC
Cuts at: 1216 1996 1216
Size: 780 4178
Acc65I G'GTAC_C
Cuts at: 1974 1974
Size: 4958
AclI AA'CG_TT
Cuts at: 4798 4798
Size: 4958
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1037 2783 3215 3568 4230 1037
Size: 1746 432 353 662 1765
Fragments arranged by size:
1765 1746 662 432 353
AflIII A'CryG_T
Cuts at: 1260 2310 4110 4391 1260
Size: 1050 1800 281 1827
Fragments arranged by size:
1827 1800 1050 281
AleI CACnn'nnGTG
Cuts at: 283 844 283
Size: 561 4397
AlwNI CAG_nnn'CTG
Cuts at: 2263 3701 2263
Size: 1438 3520
ApaLI G'TGCA_C
Cuts at: 1312 3796 1312
Size: 2484 2474
ApoI r'AATT_y
Cuts at: 606 1711 1962 4311 4567 606
Size: 1105 251 2349 256 997
Fragments arranged by size:
2349 1105 997 256 251
AseI AT'TA_AT
Cuts at: 20 20
Size: 4958
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4958
AvaI C'yCGr_G
Cuts at: 1094 1978 1094
Size: 884 4074
BamHI G'GATC_C
Cuts at: 298 431 1790 1983 298
Size: 133 1359 193 3273
Fragments arranged by size:
3273 1359 193 133
BanI G'GyrC_C
Cuts at: 1974 2388 2636 2671 1974
Size: 414 248 35 4261
Fragments arranged by size:
4261 414 248 35
BanII G_rGCy'C
Cuts at: 184 984 1368 1442 1828 1972 3002 184
Size: 800 384 74 386 144 1030 2140
Fragments arranged by size:
2140 1030 800 386 384 144 74
BbeI G_GCGC'C
Cuts at: 2392 2640 2392
Size: 248 4710
BbsI GAAGACnn'nnnn_
Cuts at: 484 871 1775 484
Size: 387 904 3667
Fragments arranged by size:
3667 904 387
BbvCI CC'TCA_GC
Cuts at: 167 167
Size: 4958
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1348 1382 1348
Size: 34 4924
BciVI GTATCCnnnnn_n'
Cuts at: 2856 3912 4958 2856
Size: 1056 1046 2856
Fragments arranged by size:
2856 1056 1046
BclI T'GATC_A
Cuts at: 2478 2478
Size: 4958
BfrBI ATG'CAT
Cuts at: 1642 1642
Size: 4958
BglI GCCn_nnn'nGGC
Cuts at: 1387 1387
Size: 4958
BglII A'GATC_T
Cuts at: 2473 4510 2473
Size: 2037 2921
BlpI GC'TnA_GC
Cuts at: 2070 2070
Size: 4958
Bme1580I G_kGCm'C
Cuts at: 991 1316 1470 2583 2676 3800 991
Size: 325 154 1113 93 1124 2149
Fragments arranged by size:
2149 1124 1113 325 154 93
BmgBI CAC'GTC
Cuts at: 1197 1263 1197
Size: 66 4892
BmrI ACTGGGnnnn_n'
Cuts at: 356 481 1280 1371 1455 2170 2251 2586 4730
Size: 125 799 91 84 715 81 335 2144
Cuts at: 4730 356
Size: 584
Fragments arranged by size:
2144 799 715 584 335 125 91 84
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3325 3325
Size: 4958
Bpu10I CC'TnA_GC
Cuts at: 167 1110 167
Size: 943 4015
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1888 2134 3013 3727 4025 1888
Size: 246 879 714 298 2821
Fragments arranged by size:
2821 879 714 298 246
BsaI GGTCTCn'nnnn_
Cuts at: 1202 1864 4662 1202
Size: 662 2798 1498
Fragments arranged by size:
2798 1498 662
BsaAI yAC'GTr
Cuts at: 925 2311 925
Size: 1386 3572
BsaBI GATnn'nnATC
Cuts at: 6 2472 4509 6
Size: 2466 2037 455
Fragments arranged by size:
2466 2037 455
BsaHI Gr'CG_yC
Cuts at: 290 485 2389 2637 3339 4835 290
Size: 195 1904 248 702 1496 413
Fragments arranged by size:
1904 1496 702 413 248 195
BsaWI w'CCGG_w
Cuts at: 295 659 803 1553 1939 2147 2668 3346 3757
Size: 364 144 750 386 208 521 678 411
Cuts at: 3757 3904 4178 295
Size: 147 274 1075
Fragments arranged by size:
1075 750 678 521 411 386 364 274
208 147 144
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 520 550 520
Size: 30 4928
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1131 1196 1131
Size: 65 4893
BseYI C'CCAG_C
Cuts at: 1377 2383 3141 3806 4214 1377
Size: 1006 758 665 408 2121
Fragments arranged by size:
2121 1006 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 745
Size: 4958
BsiEI CG_ry'CG
Cuts at: 104 543 2500 2546 3776 104
Size: 439 1957 46 1230 1286
Fragments arranged by size:
1957 1286 1230 439 46
BsiHKAI G_wGCw'C
Cuts at: 984 1316 1442 1577 1828 1972 2750 2940 3800
Size: 332 126 135 251 144 778 190 860
Cuts at: 3800 984
Size: 2142
Fragments arranged by size:
2142 860 778 332 251 190 144 135
126
BsmI GAATG_Cn'
Cuts at: 173 173
Size: 4958
Bsp1286I G_dGCh'C
Cuts at: 184 984 991 1316 1368 1442 1470 1577 1828
Size: 800 7 325 52 74 28 107 251
Cuts at: 1828 1972 2583 2676 2750 2940 3002 3800 184
Size: 144 611 93 74 190 62 798 1342
Fragments arranged by size:
1342 800 798 611 325 251 190 144
107 93 74 74 62 52 28 7
BspEI T'CCGG_A
Cuts at: 295 803 295
Size: 508 4450
BspHI T'CATG_A
Cuts at: 1475 3390 1475
Size: 1915 3043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 198 1523 2008 2222 2524 2905 198
Size: 1325 485 214 302 381 2251
Fragments arranged by size:
2251 1325 485 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3250 3304 4788 4953 50
Size: 3200 54 1484 165 55
Fragments arranged by size:
3200 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1875 2870 1875
Size: 995 3963
BsrFI r'CCGG_y
Cuts at: 680 1806 2956 3137 680
Size: 1126 1150 181 2501
Fragments arranged by size:
2501 1150 1126 181
BssSI C'ACGA_G
Cuts at: 3229 3937 3229
Size: 708 4250
BstAPI GCAn_nnn'nTGC
Cuts at: 2335 2335
Size: 4958
BstBI TT'CG_AA
Cuts at: 3319 3319
Size: 4958
BstEII G'GTnAC_C
Cuts at: 1549 1549
Size: 4958
BstXI CCAn_nnnn'nTGG
Cuts at: 846 1494 846
Size: 648 4310
BstYI r'GATC_y
Cuts at: 1 298 431 1790 1983 2450 2473 2808 3054
Size: 297 133 1359 193 467 23 335 246
Cuts at: 3054 3360 3372 3458 4510 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1359 1052 467 449 335 306 297 246
193 133 86 23 12
BtgI C'CryG_G
Cuts at: 950 950
Size: 4958
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2908 3089 3252 2908
Size: 181 163 4614
Fragments arranged by size:
4614 181 163
BtsI GCAGTG_nn'
Cuts at: 290 2201 290
Size: 1911 3047
DraI TTT'AAA
Cuts at: 1957 1957
Size: 4958
DrdI GACnn_nn'nnGTC
Cuts at: 418 875 1261 2664 4008 418
Size: 457 386 1403 1344 1368
Fragments arranged by size:
1403 1368 1344 457 386
EaeI y'GGCC_r
Cuts at: 540 947 1929 2543 2717 3108 3135 4480 540
Size: 407 982 614 174 391 27 1345 1018
Fragments arranged by size:
1345 1018 982 614 407 391 174 27
EagI C'GGCC_G
Cuts at: 540 2543 540
Size: 2003 2955
EarI CTCTTCn'nnn_
Cuts at: 1149 1447 2981 3191 1149
Size: 298 1534 210 2916
Fragments arranged by size:
2916 1534 298 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3166 3908 4054 4768 3166
Size: 742 146 714 3356
Fragments arranged by size:
3356 742 714 146
EcoICRI GAG'CTC
Cuts at: 982 1440 1826 1970 982
Size: 458 386 144 3970
Fragments arranged by size:
3970 458 386 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1037 2783 3215 3568 4230 1037
Size: 1746 432 353 662 1765
Fragments arranged by size:
1765 1746 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1240 2097 1240
Size: 857 4101
EcoRI G'AATT_C
Cuts at: 1962 1962
Size: 4958
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1049 1558 2977 1049
Size: 509 1419 3030
Fragments arranged by size:
3030 1419 509
FspI TGC'GCA
Cuts at: 2739 2739
Size: 4958
HaeII r_GCGC'y
Cuts at: 386 2392 2640 3870 4641 386
Size: 2006 248 1230 771 703
Fragments arranged by size:
2006 1230 771 703 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 251 283 439 471 520 552 819 851 1060
Size: 32 156 32 49 32 267 32 209
Cuts at: 1060 1092 1126 1158 1175 1207 1318 1350 2800
Size: 32 34 32 17 32 111 32 1450
Cuts at: 2800 2830 2832 2862 251
Size: 30 2 30 2347
Fragments arranged by size:
2347 1450 267 209 156 111 49 34
32 32 32 32 32 32 32 32
30 30 17 2
HincII GTy'rAC
Cuts at: 1217 1509 1997 1217
Size: 292 488 4178
Fragments arranged by size:
4178 488 292
HindIII A'AGCT_T
Cuts at: 1152 1152
Size: 4958
KasI G'GCGC_C
Cuts at: 2388 2636 2388
Size: 248 4710
KpnI G_GTAC'C
Cuts at: 1978 1978
Size: 4958
MfeI C'AATT_G
Cuts at: 771 771
Size: 4958
MluI A'CGCG_T
Cuts at: 4391 4391
Size: 4958
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1853 3717 3901 1853
Size: 1864 184 2910
Fragments arranged by size:
2910 1864 184
MscI TGG'CCA
Cuts at: 949 1931 2719 949
Size: 982 788 3188
Fragments arranged by size:
3188 982 788
MslI CAynn'nnrTG
Cuts at: 283 712 844 2947 3074 283
Size: 429 132 2103 127 2167
Fragments arranged by size:
2167 2103 429 132 127
MspA1I CmG'CkG
Cuts at: 492 620 668 2069 2383 2743 3527 3772 492
Size: 128 48 1401 314 360 784 245 1678
Fragments arranged by size:
1678 1401 784 360 314 245 128 48
NaeI GCC'GGC
Cuts at: 682 1808 3139 682
Size: 1126 1331 2501
Fragments arranged by size:
2501 1331 1126
NarI GG'CG_CC
Cuts at: 2389 2637 2389
Size: 248 4710
NgoMIV G'CCGG_C
Cuts at: 680 1806 3137 680
Size: 1126 1331 2501
Fragments arranged by size:
2501 1331 1126
NsiI A_TGCA'T
Cuts at: 1644 1644
Size: 4958
NspI r_CATG'y
Cuts at: 2011 2511 4114 2011
Size: 500 1603 2855
Fragments arranged by size:
2855 1603 500
PciI A'CATG_T
Cuts at: 4110 4110
Size: 4958
PfoI T'CCnGG_A
Cuts at: 1529 1529
Size: 4958
PmeI GTTT'AAAC
Cuts at: 1957 1957
Size: 4958
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 4926
PshAI GACnn'nnGTC
Cuts at: 484 484
Size: 4958
PstI C_TGCA'G
Cuts at: 1520 1592 2005 2690 1520
Size: 72 413 685 3788
Fragments arranged by size:
3788 685 413 72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4958
PvuII CAG'CTG
Cuts at: 620 2383 2743 620
Size: 1763 360 2835
Fragments arranged by size:
2835 1763 360
RsrII CG'GwC_CG
Cuts at: 3153 3153
Size: 4958
SacI G_AGCT'C
Cuts at: 984 1442 1828 1972 984
Size: 458 386 144 3970
Fragments arranged by size:
3970 458 386 144
SalI G'TCGA_C
Cuts at: 1215 1995 1215
Size: 780 4178
SapI GCTCTTCn'nnn_
Cuts at: 1447 2981 3191 1447
Size: 1534 210 3214
Fragments arranged by size:
3214 1534 210
SbfI CC_TGCA'GG
Cuts at: 2005 2005
Size: 4958
ScaI AGT'ACT
Cuts at: 511 4475 511
Size: 3964 994
SfcI C'TryA_G
Cuts at: 33 155 615 1516 1588 1655 2001 2686 3654
Size: 122 460 901 72 67 346 685 968
Cuts at: 3654 3845 33
Size: 191 1146
Fragments arranged by size:
1146 968 901 685 460 346 191 122
72 67
SfoI GGC'GCC
Cuts at: 2390 2638 2390
Size: 248 4710
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4958
SmaI CCC'GGG
Cuts at: 1980 1980
Size: 4958
SmlI C'TyrA_G
Cuts at: 1903 2113 3028 3742 4004 1903
Size: 210 915 714 262 2857
Fragments arranged by size:
2857 915 714 262 210
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4958
SphI G_CATG'C
Cuts at: 2011 2511 2011
Size: 500 4458
StyI C'CwwG_G
Cuts at: 2092 2446 2092
Size: 354 4604
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1236 1358 2541 2674 3133 3342 1236
Size: 122 1183 133 459 209 2852
Fragments arranged by size:
2852 1183 459 209 133 122
TatI w'GTAC_w
Cuts at: 509 4473 509
Size: 3964 994
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4475 4507 4475
Size: 32 4926
Tth111I GACn'n_nGTC
Cuts at: 2755 2755
Size: 4958
XbaI T'CTAG_A
Cuts at: 1989 1989
Size: 4958
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2453 2453
Size: 4958
XmaI C'CCGG_G
Cuts at: 1978 1978
Size: 4958
XmnI GAAnn'nnTTC
Cuts at: 742 742
Size: 4958
ZraI GAC'GTC
Cuts at: 486 486
Size: 4958
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BmtI BpmI BsiWI BsmBI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI NcoI NdeI NheI NotI NruI PacI
PasI PflMI PmlI PpuMI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI