Restriction map for
pF1KB5567
(Circular) (Six-base) MAPSORT of: pF1KB5567.seq Check: 3627 from: 1 to: 5129
pF1KB5567 5129 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 2167 2167
Size: 5129
Acc65I G'GTAC_C
Cuts at: 2145 2145
Size: 5129
AclI AA'CG_TT
Cuts at: 4969 4969
Size: 5129
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1148 1297 2954 3386 3739 4401 1148
Size: 149 1657 432 353 662 1876
Fragments arranged by size:
1876 1657 662 432 353 149
AfeI AGC'GCT
Cuts at: 1726 1726
Size: 5129
AflII C'TTAA_G
Cuts at: 1236 1236
Size: 5129
AflIII A'CryG_T
Cuts at: 237 2481 4281 4562 237
Size: 2244 1800 281 804
Fragments arranged by size:
2244 1800 804 281
AlwNI CAG_nnn'CTG
Cuts at: 1141 1705 2434 3872 1141
Size: 564 729 1438 2398
Fragments arranged by size:
2398 1438 729 564
ApaI G_GGCC'C
Cuts at: 1621 1621
Size: 5129
ApaLI G'TGCA_C
Cuts at: 1034 3967 1034
Size: 2933 2196
ApoI r'AATT_y
Cuts at: 1244 1572 1759 2086 2133 4482 4738 1244
Size: 328 187 327 47 2349 256 1635
Fragments arranged by size:
2349 1635 328 327 256 187 47
AseI AT'TA_AT
Cuts at: 20 20
Size: 5129
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5129
AvaI C'yCGr_G
Cuts at: 2149 2149
Size: 5129
BamHI G'GATC_C
Cuts at: 2154 2154
Size: 5129
BanI G'GyrC_C
Cuts at: 813 2145 2559 2807 2842 813
Size: 1332 414 248 35 3100
Fragments arranged by size:
3100 1332 414 248 35
BanII G_rGCy'C
Cuts at: 575 1621 1661 2143 3173 575
Size: 1046 40 482 1030 2531
Fragments arranged by size:
2531 1046 1030 482 40
BbeI G_GCGC'C
Cuts at: 2563 2811 2563
Size: 248 4881
BbsI GAAGACnn'nnnn_
Cuts at: 946 1154 1350 1538 946
Size: 208 196 188 4537
Fragments arranged by size:
4537 208 196 188
BciVI GTATCCnnnnn_n'
Cuts at: 3027 4083 5129 3027
Size: 1056 1046 3027
Fragments arranged by size:
3027 1056 1046
BclI T'GATC_A
Cuts at: 2649 2649
Size: 5129
BglII A'GATC_T
Cuts at: 112 2644 4681 112
Size: 2532 2037 560
Fragments arranged by size:
2532 2037 560
BlpI GC'TnA_GC
Cuts at: 2241 2241
Size: 5129
Bme1580I G_kGCm'C
Cuts at: 816 1038 1621 2754 2847 3971 816
Size: 222 583 1133 93 1124 1974
Fragments arranged by size:
1974 1133 1124 583 222 93
BmrI ACTGGGnnnn_n'
Cuts at: 1348 2341 2422 2757 4901 1348
Size: 993 81 335 2144 1576
Fragments arranged by size:
2144 1576 993 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3496 3496
Size: 5129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1575 1985 1105
Size: 470 410 4249
Fragments arranged by size:
4249 470 410
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 918 1539 1666 2305 3184 3898 4196 918
Size: 621 127 639 879 714 298 1851
Fragments arranged by size:
1851 879 714 639 621 298 127
BsaI GGTCTCn'nnnn_
Cuts at: 445 968 1684 4833 445
Size: 523 716 3149 741
Fragments arranged by size:
3149 741 716 523
BsaAI yAC'GTr
Cuts at: 1434 2482 1434
Size: 1048 4081
BsaBI GATnn'nnATC
Cuts at: 6 753 2643 4680 6
Size: 747 1890 2037 455
Fragments arranged by size:
2037 1890 747 455
BsaHI Gr'CG_yC
Cuts at: 706 2560 2808 3510 5006 706
Size: 1854 248 702 1496 829
Fragments arranged by size:
1854 1496 829 702 248
BsaWI w'CCGG_w
Cuts at: 180 666 2318 2839 3517 3928 4075 4349 180
Size: 486 1652 521 678 411 147 274 960
Fragments arranged by size:
1652 960 678 521 486 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 632 662 1813 1843 1952 1968 1982 1998 632
Size: 30 1151 30 109 16 14 16 3763
Fragments arranged by size:
3763 1151 109 30 30 16 16 14
BseYI C'CCAG_C
Cuts at: 886 1156 2554 3312 3977 4385 886
Size: 270 1398 758 665 408 1630
Fragments arranged by size:
1630 1398 758 665 408 270
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1823 1823
Size: 5129
BsiEI CG_ry'CG
Cuts at: 104 2671 2717 3947 104
Size: 2567 46 1230 1286
Fragments arranged by size:
2567 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 231 1038 1661 2143 2921 3111 3971 231
Size: 807 623 482 778 190 860 1389
Fragments arranged by size:
1389 860 807 778 623 482 190
BsmI GAATG_Cn'
Cuts at: 776 1281 776
Size: 505 4624
BsmBI CGTCTCn'nnnn_
Cuts at: 713 894 713
Size: 181 4948
Bsp1286I G_dGCh'C
Cuts at: 231 575 816 1038 1621 1661 2143 2754 2847
Size: 344 241 222 583 40 482 611 93
Cuts at: 2847 2921 3111 3173 3971 231
Size: 74 190 62 798 1389
Fragments arranged by size:
1389 798 611 583 482 344 241 222
190 93 74 62 40
BspEI T'CCGG_A
Cuts at: 180 666 180
Size: 486 4643
BspHI T'CATG_A
Cuts at: 957 3561 957
Size: 2604 2525
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1762 2179 2393 2695 3076 1762
Size: 417 214 302 381 3815
Fragments arranged by size:
3815 417 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3421 3475 4959 5124 50
Size: 3371 54 1484 165 55
Fragments arranged by size:
3371 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1463 3041 1463
Size: 1578 3551
BsrFI r'CCGG_y
Cuts at: 3127 3308 3127
Size: 181 4948
BssSI C'ACGA_G
Cuts at: 3400 4108 3400
Size: 708 4421
BstAPI GCAn_nnn'nTGC
Cuts at: 1042 2506 1042
Size: 1464 3665
BstBI TT'CG_AA
Cuts at: 3490 3490
Size: 5129
BstXI CCAn_nnnn'nTGG
Cuts at: 2064 2064
Size: 5129
BstYI r'GATC_y
Cuts at: 1 112 660 2154 2621 2644 2979 3225 3531
Size: 111 548 1494 467 23 335 246 306
Cuts at: 3531 3543 3629 4681 1
Size: 12 86 1052 449
Fragments arranged by size:
1494 1052 548 467 449 335 306 246
111 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1401 1877 1401
Size: 476 4653
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3079 3260 3423 3079
Size: 181 163 4785
Fragments arranged by size:
4785 181 163
BtsI GCAGTG_nn'
Cuts at: 2372 2372
Size: 5129
DraI TTT'AAA
Cuts at: 2128 2128
Size: 5129
DrdI GACnn_nn'nnGTC
Cuts at: 2835 4179 2835
Size: 1344 3785
EaeI y'GGCC_r
Cuts at: 829 2714 2888 3279 3306 4651 829
Size: 1885 174 391 27 1345 1307
Fragments arranged by size:
1885 1345 1307 391 174 27
EagI C'GGCC_G
Cuts at: 2714 2714
Size: 5129
EarI CTCTTCn'nnn_
Cuts at: 503 716 1668 1935 3152 3362 503
Size: 213 952 267 1217 210 2270
Fragments arranged by size:
2270 1217 952 267 213 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3337 4079 4225 4939 3337
Size: 742 146 714 3527
Fragments arranged by size:
3527 742 714 146
EcoICRI GAG'CTC
Cuts at: 1659 2141 1659
Size: 482 4647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1148 1297 1575 1985 2954 3386 3739 4401
Size: 43 149 278 410 969 432 353 662
Cuts at: 4401 1105
Size: 1833
Fragments arranged by size:
1833 969 662 432 410 353 278 149
43
EcoO109I rG'GnC_Cy
Cuts at: 946 1617 1618 2268 946
Size: 671 1 650 3807
Fragments arranged by size:
3807 671 650 1
EcoRI G'AATT_C
Cuts at: 2133 2133
Size: 5129
EcoRV GAT'ATC
Cuts at: 1465 1465
Size: 5129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 496 1979 3148 496
Size: 1483 1169 2477
Fragments arranged by size:
2477 1483 1169
FspI TGC'GCA
Cuts at: 2910 2910
Size: 5129
HaeII r_GCGC'y
Cuts at: 887 1728 2563 2811 4041 4812 887
Size: 841 835 248 1230 771 1204
Fragments arranged by size:
1230 1204 841 835 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 645 677 1278 1310 1498 1530 1860 1892 1968
Size: 32 601 32 188 32 330 32 76
Cuts at: 1968 2000 2971 3001 3003 3033 645
Size: 32 971 30 2 30 2741
Fragments arranged by size:
2741 971 601 330 188 76 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2168 2168
Size: 5129
KasI G'GCGC_C
Cuts at: 2559 2807 2559
Size: 248 4881
KpnI G_GTAC'C
Cuts at: 2149 2149
Size: 5129
MluI A'CGCG_T
Cuts at: 4562 4562
Size: 5129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 233 325 1492 2116 3888 4072 233
Size: 92 1167 624 1772 184 1290
Fragments arranged by size:
1772 1290 1167 624 184 92
MscI TGG'CCA
Cuts at: 831 2890 831
Size: 2059 3070
MslI CAynn'nnrTG
Cuts at: 3118 3245 3118
Size: 127 5002
MspA1I CmG'CkG
Cuts at: 1903 1981 2035 2240 2554 2914 3698 3943 1903
Size: 78 54 205 314 360 784 245 3089
Fragments arranged by size:
3089 784 360 314 245 205 78 54
NaeI GCC'GGC
Cuts at: 3310 3310
Size: 5129
NarI GG'CG_CC
Cuts at: 2560 2808 2560
Size: 248 4881
NdeI CA'TA_TG
Cuts at: 1392 2019 1392
Size: 627 4502
NgoMIV G'CCGG_C
Cuts at: 3308 3308
Size: 5129
NspI r_CATG'y
Cuts at: 241 721 1045 2182 2682 4285 241
Size: 480 324 1137 500 1603 1085
Fragments arranged by size:
1603 1137 1085 500 480 324
PciI A'CATG_T
Cuts at: 237 4281 237
Size: 4044 1085
PflMI CCAn_nnn'nTGG
Cuts at: 150 2008 150
Size: 1858 3271
PmeI GTTT'AAAC
Cuts at: 2128 2128
Size: 5129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3575 3607 3575
Size: 32 5097
PpuMI rG'GwC_Cy
Cuts at: 946 946
Size: 5129
PsiI TTA'TAA
Cuts at: 553 553
Size: 5129
PspOMI G'GGCC_C
Cuts at: 1617 1617
Size: 5129
PstI C_TGCA'G
Cuts at: 1914 2176 2861 1914
Size: 262 685 4182
Fragments arranged by size:
4182 685 262
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5129
PvuII CAG'CTG
Cuts at: 1903 1981 2035 2554 2914 1903
Size: 78 54 519 360 4118
Fragments arranged by size:
4118 519 360 78 54
RsrII CG'GwC_CG
Cuts at: 3324 3324
Size: 5129
SacI G_AGCT'C
Cuts at: 1661 2143 1661
Size: 482 4647
SalI G'TCGA_C
Cuts at: 2166 2166
Size: 5129
SapI GCTCTTCn'nnn_
Cuts at: 3152 3362 3152
Size: 210 4919
SbfI CC_TGCA'GG
Cuts at: 2176 2176
Size: 5129
ScaI AGT'ACT
Cuts at: 4646 4646
Size: 5129
SfcI C'TryA_G
Cuts at: 33 857 1370 1910 2172 2857 3825 4016 33
Size: 824 513 540 262 685 968 191 1146
Fragments arranged by size:
1146 968 824 685 540 513 262 191
SfoI GGC'GCC
Cuts at: 2561 2809 2561
Size: 248 4881
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5129
SmaI CCC'GGG
Cuts at: 2151 2151
Size: 5129
SmlI C'TyrA_G
Cuts at: 897 1236 1554 1681 2284 3199 3913 4175 897
Size: 339 318 127 603 915 714 262 1851
Fragments arranged by size:
1851 915 714 603 339 318 262 127
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5129
SphI G_CATG'C
Cuts at: 1045 2182 2682 1045
Size: 1137 500 3492
Fragments arranged by size:
3492 1137 500
StuI AGG'CCT
Cuts at: 895 1305 895
Size: 410 4719
StyI C'CwwG_G
Cuts at: 329 701 1817 2263 2617 329
Size: 372 1116 446 354 2841
Fragments arranged by size:
2841 1116 446 372 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2712 2845 3304 3513 2712
Size: 133 459 209 4328
Fragments arranged by size:
4328 459 209 133
TatI w'GTAC_w
Cuts at: 4644 4644
Size: 5129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 339 371 4646 4678 339
Size: 32 4275 32 790
Fragments arranged by size:
4275 790 32 32
Tth111I GACn'n_nGTC
Cuts at: 2926 2926
Size: 5129
XbaI T'CTAG_A
Cuts at: 2160 2160
Size: 5129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 824 2624 824
Size: 1800 3329
XmaI C'CCGG_G
Cuts at: 2149 2149
Size: 5129
XmnI GAAnn'nnTTC
Cuts at: 197 197
Size: 5129
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AgeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BseRI BsiWI BsrGI BssHII BstEII BstZ17I BtgI
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NcoI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI