Restriction map for
pF1KB5562
(Circular) (Six-base) MAPSORT of: pF1KB5562.seq Check: 129 from: 1 to: 5474
pF1KB5562 5474 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1051 2043 1051
Size: 992 4482
AccI GT'mk_AC
Cuts at: 2512 2512
Size: 5474
Acc65I G'GTAC_C
Cuts at: 453 1644 2490 453
Size: 1191 846 3437
Fragments arranged by size:
3437 1191 846
AclI AA'CG_TT
Cuts at: 5314 5314
Size: 5474
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 773 1334 2112 3299 3731 4084 4746 773
Size: 561 778 1187 432 353 662 1501
Fragments arranged by size:
1501 1187 778 662 561 432 353
AflIII A'CryG_T
Cuts at: 2331 2826 4626 4907 2331
Size: 495 1800 281 2898
Fragments arranged by size:
2898 1800 495 281
AleI CACnn'nnGTG
Cuts at: 1231 1244 1495 1231
Size: 13 251 5210
Fragments arranged by size:
5210 251 13
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 371 371
Size: 5474
AlwNI CAG_nnn'CTG
Cuts at: 597 940 1093 1951 2779 4217 597
Size: 343 153 858 828 1438 1854
Fragments arranged by size:
1854 1438 858 828 343 153
ApaLI G'TGCA_C
Cuts at: 745 4312 745
Size: 3567 1907
ApoI r'AATT_y
Cuts at: 194 280 1300 1902 2431 2478 4827 5083 194
Size: 86 1020 602 529 47 2349 256 585
Fragments arranged by size:
2349 1020 602 585 529 256 86 47
AseI AT'TA_AT
Cuts at: 20 20
Size: 5474
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5474
AvaI C'yCGr_G
Cuts at: 2494 2494
Size: 5474
BamHI G'GATC_C
Cuts at: 2499 2499
Size: 5474
BanI G'GyrC_C
Cuts at: 453 1605 1644 2149 2490 2904 3152 3187 453
Size: 1152 39 505 341 414 248 35 2740
Fragments arranged by size:
2740 1152 505 414 341 248 39 35
BanII G_rGCy'C
Cuts at: 355 883 1594 2488 3518 355
Size: 528 711 894 1030 2311
Fragments arranged by size:
2311 1030 894 711 528
BbeI G_GCGC'C
Cuts at: 2908 3156 2908
Size: 248 5226
BbsI GAAGACnn'nnnn_
Cuts at: 851 1322 2367 851
Size: 471 1045 3958
Fragments arranged by size:
3958 1045 471
BciVI GTATCCnnnnn_n'
Cuts at: 823 3372 4428 5474 823
Size: 2549 1056 1046 823
Fragments arranged by size:
2549 1056 1046 823
BclI T'GATC_A
Cuts at: 300 588 738 2994 300
Size: 288 150 2256 2780
Fragments arranged by size:
2780 2256 288 150
BglI GCCn_nnn'nGGC
Cuts at: 112 2286 112
Size: 2174 3300
BglII A'GATC_T
Cuts at: 2989 5026 2989
Size: 2037 3437
BlpI GC'TnA_GC
Cuts at: 952 2586 952
Size: 1634 3840
Bme1580I G_kGCm'C
Cuts at: 471 749 2154 3099 3192 4316 471
Size: 278 1405 945 93 1124 1629
Fragments arranged by size:
1629 1405 1124 945 278 93
BmrI ACTGGGnnnn_n'
Cuts at: 166 928 2085 2686 2767 3102 5246 166
Size: 762 1157 601 81 335 2144 394
Fragments arranged by size:
2144 1157 762 601 394 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 369 1215 1608 3841 369
Size: 846 393 2233 2002
Fragments arranged by size:
2233 2002 846 393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1603 1812 1603
Size: 209 5265
Bpu10I CC'TnA_GC
Cuts at: 1593 2034 1593
Size: 441 5033
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 867 1753 2650 3529 4243 4541 867
Size: 886 897 879 714 298 1800
Fragments arranged by size:
1800 897 886 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5178 5178
Size: 5474
BsaAI yAC'GTr
Cuts at: 2827 2827
Size: 5474
BsaBI GATnn'nnATC
Cuts at: 6 299 1766 2988 5025 6
Size: 293 1467 1222 2037 455
Fragments arranged by size:
2037 1467 1222 455 293
BsaHI Gr'CG_yC
Cuts at: 2905 3153 3855 5351 2905
Size: 248 702 1496 3028
Fragments arranged by size:
3028 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1388 1394 2663 3184 3862 4273 4420 4694 1388
Size: 6 1269 521 678 411 147 274 2168
Fragments arranged by size:
2168 1269 678 521 411 274 147 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 200 230 1134 1164 1863 1893 2374 2404 200
Size: 30 904 30 699 30 481 30 3270
Fragments arranged by size:
3270 904 699 481 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 221 1815 221
Size: 1594 3880
BseYI C'CCAG_C
Cuts at: 1056 2048 2397 2899 3657 4322 4730 1056
Size: 992 349 502 758 665 408 1800
Fragments arranged by size:
1800 992 758 665 502 408 349
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1014 1014
Size: 5474
BsiEI CG_ry'CG
Cuts at: 104 1894 3016 3062 4292 104
Size: 1790 1122 46 1230 1286
Fragments arranged by size:
1790 1286 1230 1122 46
BsiHKAI G_wGCw'C
Cuts at: 355 749 2488 3266 3456 4316 355
Size: 394 1739 778 190 860 1513
Fragments arranged by size:
1739 1513 860 778 394 190
Bsp1286I G_dGCh'C
Cuts at: 355 471 749 883 1594 2154 2488 3099 3192
Size: 116 278 134 711 560 334 611 93
Cuts at: 3192 3266 3456 3518 4316 355
Size: 74 190 62 798 1513
Fragments arranged by size:
1513 798 711 611 560 334 278 190
134 116 93 74 62
BspHI T'CATG_A
Cuts at: 3906 3906
Size: 5474
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1051 1128 2043 2524 2738 3040 3421 1051
Size: 77 915 481 214 302 381 3104
Fragments arranged by size:
3104 915 481 381 302 214 77
BsrBI CCG'CTC
Cuts at: 50 324 2033 3766 3820 5304 5469 50
Size: 274 1709 1733 54 1484 165 55
Fragments arranged by size:
1733 1709 1484 274 165 55 54
BsrDI GCAATG_nn'
Cuts at: 167 3386 167
Size: 3219 2255
BsrFI r'CCGG_y
Cuts at: 1893 3472 3653 1893
Size: 1579 181 3714
Fragments arranged by size:
3714 1579 181
BsrGI T'GTAC_A
Cuts at: 1159 1159
Size: 5474
BssSI C'ACGA_G
Cuts at: 3745 4453 3745
Size: 708 4766
BstAPI GCAn_nnn'nTGC
Cuts at: 1112 2851 1112
Size: 1739 3735
BstBI TT'CG_AA
Cuts at: 3835 3835
Size: 5474
BstYI r'GATC_y
Cuts at: 1 819 1767 2203 2499 2966 2989 3324 3570
Size: 818 948 436 296 467 23 335 246
Cuts at: 3570 3876 3888 3974 5026 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 948 818 467 449 436 335 306
296 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2369 2369
Size: 5474
BtgI C'CryG_G
Cuts at: 107 1519 107
Size: 1412 4062
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2147 3424 3605 3768 2147
Size: 1277 181 163 3853
Fragments arranged by size:
3853 1277 181 163
BtsI GCAGTG_nn'
Cuts at: 1026 2717 1026
Size: 1691 3783
DraI TTT'AAA
Cuts at: 2473 2473
Size: 5474
DraIII CAC_nnn'GTG
Cuts at: 1017 1017
Size: 5474
DrdI GACnn_nn'nnGTC
Cuts at: 3180 4524 3180
Size: 1344 4130
EaeI y'GGCC_r
Cuts at: 1095 1121 1664 1891 2278 2361 3059 3233 3624
Size: 26 543 227 387 83 698 174 391
Cuts at: 3624 3651 4996 1095
Size: 27 1345 1573
Fragments arranged by size:
1573 1345 698 543 391 387 227 174
83 27 26
EagI C'GGCC_G
Cuts at: 1891 3059 1891
Size: 1168 4306
EarI CTCTTCn'nnn_
Cuts at: 1647 3497 3707 1647
Size: 1850 210 3414
Fragments arranged by size:
3414 1850 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3682 4424 4570 5284 3682
Size: 742 146 714 3872
Fragments arranged by size:
3872 742 714 146
EcoICRI GAG'CTC
Cuts at: 353 2486 353
Size: 2133 3341
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 773 1334 1603 1812 2112 3299 3731 4084 4746
Size: 561 269 209 300 1187 432 353 662
Cuts at: 4746 773
Size: 1501
Fragments arranged by size:
1501 1187 662 561 432 353 300 269
209
EcoO109I rG'GnC_Cy
Cuts at: 1561 2613 1561
Size: 1052 4422
EcoRI G'AATT_C
Cuts at: 194 1300 2431 2478 194
Size: 1106 1131 47 3190
Fragments arranged by size:
3190 1131 1106 47
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 388 3493 388
Size: 3105 2369
FspI TGC'GCA
Cuts at: 3255 3255
Size: 5474
HaeII r_GCGC'y
Cuts at: 2908 3156 4386 5157 2908
Size: 248 1230 771 3225
Fragments arranged by size:
3225 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 200 232 292 324 2374 2406 3316
Size: 32 32 32 60 32 2050 32 910
Cuts at: 3316 3346 3348 3378 136
Size: 30 2 30 2232
Fragments arranged by size:
2232 2050 910 60 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1183 2513 1183
Size: 1330 4144
KasI G'GCGC_C
Cuts at: 2904 3152 2904
Size: 248 5226
KpnI G_GTAC'C
Cuts at: 457 1648 2494 457
Size: 1191 846 3437
Fragments arranged by size:
3437 1191 846
MluI A'CGCG_T
Cuts at: 4907 4907
Size: 5474
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 317 1368 1559 2012 4233 4417 317
Size: 1051 191 453 2221 184 1374
Fragments arranged by size:
2221 1374 1051 453 191 184
MscI TGG'CCA
Cuts at: 1097 1123 1666 2280 2363 3235 1097
Size: 26 543 614 83 872 3336
Fragments arranged by size:
3336 872 614 543 83 26
MslI CAynn'nnrTG
Cuts at: 752 868 1231 1244 1495 3463 3590 752
Size: 116 363 13 251 1968 127 2636
Fragments arranged by size:
2636 1968 363 251 127 116 13
MspA1I CmG'CkG
Cuts at: 1244 1602 2585 2899 3259 4043 4288 1244
Size: 358 983 314 360 784 245 2430
Fragments arranged by size:
2430 983 784 360 358 314 245
NaeI GCC'GGC
Cuts at: 1895 3655 1895
Size: 1760 3714
NarI GG'CG_CC
Cuts at: 2905 3153 2905
Size: 248 5226
NcoI C'CATG_G
Cuts at: 107 107
Size: 5474
NgoMIV G'CCGG_C
Cuts at: 1893 3653 1893
Size: 1760 3714
NspI r_CATG'y
Cuts at: 2335 2527 3027 4630 2335
Size: 192 500 1603 3179
Fragments arranged by size:
3179 1603 500 192
PasI CC'CwG_GG
Cuts at: 1863 1863
Size: 5474
PciI A'CATG_T
Cuts at: 2331 4626 2331
Size: 2295 3179
PflMI CCAn_nnn'nTGG
Cuts at: 1093 2055 1093
Size: 962 4512
PfoI T'CCnGG_A
Cuts at: 815 2378 815
Size: 1563 3911
PmeI GTTT'AAAC
Cuts at: 2473 2473
Size: 5474
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1441 1473 3920 3952 1441
Size: 32 2447 32 2963
Fragments arranged by size:
2963 2447 32 32
PpuMI rG'GwC_Cy
Cuts at: 1561 1561
Size: 5474
PstI C_TGCA'G
Cuts at: 1956 2238 2521 3206 1956
Size: 282 283 685 4224
Fragments arranged by size:
4224 685 283 282
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5474
PvuII CAG'CTG
Cuts at: 1602 2899 3259 1602
Size: 1297 360 3817
Fragments arranged by size:
3817 1297 360
RsrII CG'GwC_CG
Cuts at: 3669 3669
Size: 5474
SacI G_AGCT'C
Cuts at: 355 2488 355
Size: 2133 3341
SalI G'TCGA_C
Cuts at: 2511 2511
Size: 5474
SapI GCTCTTCn'nnn_
Cuts at: 3497 3707 3497
Size: 210 5264
SbfI CC_TGCA'GG
Cuts at: 2521 2521
Size: 5474
ScaI AGT'ACT
Cuts at: 4991 4991
Size: 5474
SfcI C'TryA_G
Cuts at: 33 462 1952 2234 2517 3202 4170 4361 33
Size: 429 1490 282 283 685 968 191 1146
Fragments arranged by size:
1490 1146 968 685 429 283 282 191
SfoI GGC'GCC
Cuts at: 2906 3154 2906
Size: 248 5226
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5474
SmaI CCC'GGG
Cuts at: 2496 2496
Size: 5474
SmlI C'TyrA_G
Cuts at: 882 1732 2629 3544 4258 4520 882
Size: 850 897 915 714 262 1836
Fragments arranged by size:
1836 915 897 850 714 262
SpeI A'CTAG_T
Cuts at: 67 1144 67
Size: 1077 4397
SphI G_CATG'C
Cuts at: 2527 3027 2527
Size: 500 4974
SspI AAT'ATT
Cuts at: 1293 1293
Size: 5474
StuI AGG'CCT
Cuts at: 1048 1048
Size: 5474
StyI C'CwwG_G
Cuts at: 107 1998 2608 2962 107
Size: 1891 610 354 2619
Fragments arranged by size:
2619 1891 610 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 764 1623 1861 2011 2260 3057 3190 3649 3858
Size: 859 238 150 249 797 133 459 209
Cuts at: 3858 764
Size: 2380
Fragments arranged by size:
2380 859 797 459 249 238 209 150
133
TatI w'GTAC_w
Cuts at: 1159 1472 4989 1159
Size: 313 3517 1644
Fragments arranged by size:
3517 1644 313
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 511 543 1863 1895 4991 5023 511
Size: 32 1320 32 3096 32 962
Fragments arranged by size:
3096 1320 962 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3271 3271
Size: 5474
XbaI T'CTAG_A
Cuts at: 2505 2505
Size: 5474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 504 713 2969 504
Size: 209 2256 3009
Fragments arranged by size:
3009 2256 209
XmaI C'CCGG_G
Cuts at: 2494 2494
Size: 5474
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI
BsmI BsmBI BspEI BssHII BstEII BstXI BstZ17I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI