Restriction map for
pF1KB5559
(Circular) (Six-base) MAPSORT of: pF1KB5559.seq Check: 8623 from: 1 to: 3848
pF1KB5559 3848 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 539 886 539
Size: 347 3501
Acc65I G'GTAC_C
Cuts at: 310 864 310
Size: 554 3294
AclI AA'CG_TT
Cuts at: 3688 3688
Size: 3848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1673 2105 2852 3120 1673
Size: 432 747 268 2401
Fragments arranged by size:
2401 747 432 268
AflIII A'CryG_T
Cuts at: 1200 2304 3281 1200
Size: 1104 977 1767
Fragments arranged by size:
1767 1104 977
AgeI A'CCGG_T
Cuts at: 2236 2236
Size: 3848
AlwNI CAG_nnn'CTG
Cuts at: 1153 2720 1153
Size: 1567 2281
ApaLI G'TGCA_C
Cuts at: 2618 2618
Size: 3848
ApoI r'AATT_y
Cuts at: 749 852 3201 3457 749
Size: 103 2349 256 1140
Fragments arranged by size:
2349 1140 256 103
AseI AT'TA_AT
Cuts at: 20 20
Size: 3848
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
AvaI C'yCGr_G
Cuts at: 868 868
Size: 3848
BamHI G'GATC_C
Cuts at: 873 873
Size: 3848
BanI G'GyrC_C
Cuts at: 310 331 864 1278 1526 1561 310
Size: 21 533 414 248 35 2597
Fragments arranged by size:
2597 533 414 248 35 21
BanII G_rGCy'C
Cuts at: 862 1892 862
Size: 1030 2818
BbeI G_GCGC'C
Cuts at: 1282 1530 1282
Size: 248 3600
BciVI GTATCCnnnnn_n'
Cuts at: 1746 2507 3848 1746
Size: 761 1341 1746
Fragments arranged by size:
1746 1341 761
BclI T'GATC_A
Cuts at: 1368 1368
Size: 3848
BglII A'GATC_T
Cuts at: 1363 3400 1363
Size: 2037 1811
BlpI GC'TnA_GC
Cuts at: 960 960
Size: 3848
Bme1580I G_kGCm'C
Cuts at: 334 1473 1566 2622 334
Size: 1139 93 1056 1560
Fragments arranged by size:
1560 1139 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1060 1141 1476 3620 1060
Size: 81 335 2144 1288
Fragments arranged by size:
2144 1288 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 3848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 265
Size: 3848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1903 2395 2693 1024
Size: 879 492 298 2179
Fragments arranged by size:
2179 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3552 3552
Size: 3848
BsaAI yAC'GTr
Cuts at: 1201 1201
Size: 3848
BsaBI GATnn'nnATC
Cuts at: 6 784 1362 3399 6
Size: 778 578 2037 455
Fragments arranged by size:
2037 778 578 455
BsaHI Gr'CG_yC
Cuts at: 112 1279 1527 2229 3725 112
Size: 1167 248 702 1496 235
Fragments arranged by size:
1496 1167 702 248 235
BsaWI w'CCGG_w
Cuts at: 1037 1558 2236 2510 2657 3068 1037
Size: 521 678 274 147 411 1817
Fragments arranged by size:
1817 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1273 2031 2608 3104 1273
Size: 758 577 496 2017
Fragments arranged by size:
2017 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1390 1436 2644 104
Size: 1286 46 1208 1308
Fragments arranged by size:
1308 1286 1208 46
BsiHKAI G_wGCw'C
Cuts at: 299 862 1640 1830 2622 299
Size: 563 778 190 792 1525
Fragments arranged by size:
1525 792 778 563 190
BsmBI CGTCTCn'nnnn_
Cuts at: 125 125
Size: 3848
Bsp1286I G_dGCh'C
Cuts at: 299 334 862 1473 1566 1640 1830 1892 2622
Size: 35 528 611 93 74 190 62 730
Cuts at: 2622 299
Size: 1525
Fragments arranged by size:
1525 730 611 528 190 93 74 62
35
BspEI T'CCGG_A
Cuts at: 3068 3068
Size: 3848
BspHI T'CATG_A
Cuts at: 3024 3024
Size: 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 361 898 1112 1414 1795 361
Size: 537 214 302 381 2414
Fragments arranged by size:
2414 537 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2140 2194 3678 3843 50
Size: 2090 54 1484 165 55
Fragments arranged by size:
2090 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 299 395 1760 299
Size: 96 1365 2387
Fragments arranged by size:
2387 1365 96
BsrFI r'CCGG_y
Cuts at: 1846 2027 2236 1846
Size: 181 209 3458
Fragments arranged by size:
3458 209 181
BssSI C'ACGA_G
Cuts at: 809 2119 2477 809
Size: 1310 358 2180
Fragments arranged by size:
2180 1310 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1225 1225
Size: 3848
BstBI TT'CG_AA
Cuts at: 2209 2209
Size: 3848
BstXI CCAn_nnnn'nTGG
Cuts at: 781 781
Size: 3848
BstYI r'GATC_y
Cuts at: 1 545 873 1340 1363 1698 1944 2956 3042
Size: 544 328 467 23 335 246 1012 86
Cuts at: 3042 3054 3400 1
Size: 12 346 449
Fragments arranged by size:
1012 544 467 449 346 335 328 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3848
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1798 1979 2142 1798
Size: 181 163 3504
Fragments arranged by size:
3504 181 163
BtsI GCAGTG_nn'
Cuts at: 1091 1091
Size: 3848
DraI TTT'AAA
Cuts at: 266 847 266
Size: 581 3267
DrdI GACnn_nn'nnGTC
Cuts at: 1554 2412 1554
Size: 858 2990
EaeI y'GGCC_r
Cuts at: 1433 1607 1998 2025 3370 1433
Size: 174 391 27 1345 1911
Fragments arranged by size:
1911 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1433 1433
Size: 3848
EarI CTCTTCn'nnn_
Cuts at: 1871 2081 1871
Size: 210 3638
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2056 2366 2512 3658 2056
Size: 310 146 1146 2246
Fragments arranged by size:
2246 1146 310 146
EcoICRI GAG'CTC
Cuts at: 860 860
Size: 3848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 1673 2105 2852 3120 265
Size: 1408 432 747 268 993
Fragments arranged by size:
1408 993 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 987 987
Size: 3848
EcoRI G'AATT_C
Cuts at: 852 852
Size: 3848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 486 1867 486
Size: 1381 2467
FspI TGC'GCA
Cuts at: 1629 1629
Size: 3848
HaeII r_GCGC'y
Cuts at: 1282 1530 2552 3531 1282
Size: 248 1022 979 1599
Fragments arranged by size:
1599 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1690 1720 1722 1752 1690
Size: 30 2 30 3786
Fragments arranged by size:
3786 30 30 2
HincII GTy'rAC
Cuts at: 887 887
Size: 3848
KasI G'GCGC_C
Cuts at: 1278 1526 1278
Size: 248 3600
KpnI G_GTAC'C
Cuts at: 314 868 314
Size: 554 3294
MluI A'CGCG_T
Cuts at: 3281 3281
Size: 3848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 798 2519 2703 798
Size: 1721 184 1943
Fragments arranged by size:
1943 1721 184
MscI TGG'CCA
Cuts at: 1609 1609
Size: 3848
MslI CAynn'nnrTG
Cuts at: 566 1837 1964 566
Size: 1271 127 2450
Fragments arranged by size:
2450 1271 127
MspA1I CmG'CkG
Cuts at: 959 1273 1633 2646 2891 959
Size: 314 360 1013 245 1916
Fragments arranged by size:
1916 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2029 2029
Size: 3848
NarI GG'CG_CC
Cuts at: 1279 1527 1279
Size: 248 3600
NcoI C'CATG_G
Cuts at: 107 107
Size: 3848
NgoMIV G'CCGG_C
Cuts at: 2027 2027
Size: 3848
NspI r_CATG'y
Cuts at: 901 1401 2308 901
Size: 500 907 2441
Fragments arranged by size:
2441 907 500
PciI A'CATG_T
Cuts at: 2304 2304
Size: 3848
PmeI GTTT'AAAC
Cuts at: 847 847
Size: 3848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 3816
PsiI TTA'TAA
Cuts at: 703 703
Size: 3848
PstI C_TGCA'G
Cuts at: 134 895 1580 134
Size: 761 685 2402
Fragments arranged by size:
2402 761 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3848
PvuII CAG'CTG
Cuts at: 1273 1633 1273
Size: 360 3488
RsrII CG'GwC_CG
Cuts at: 2043 2043
Size: 3848
SacI G_AGCT'C
Cuts at: 862 862
Size: 3848
SalI G'TCGA_C
Cuts at: 885 885
Size: 3848
SapI GCTCTTCn'nnn_
Cuts at: 1871 2081 1871
Size: 210 3638
SbfI CC_TGCA'GG
Cuts at: 895 895
Size: 3848
ScaI AGT'ACT
Cuts at: 3365 3365
Size: 3848
SfcI C'TryA_G
Cuts at: 33 130 512 891 1576 2569 2760 33
Size: 97 382 379 685 993 191 1121
Fragments arranged by size:
1121 993 685 382 379 191 97
SfoI GGC'GCC
Cuts at: 1280 1528 1280
Size: 248 3600
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
SmaI CCC'GGG
Cuts at: 870 870
Size: 3848
SmlI C'TyrA_G
Cuts at: 1003 1918 2410 2672 1003
Size: 915 492 262 2179
Fragments arranged by size:
2179 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3848
SphI G_CATG'C
Cuts at: 901 1401 901
Size: 500 3348
StyI C'CwwG_G
Cuts at: 107 982 1336 107
Size: 875 354 2619
Fragments arranged by size:
2619 875 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1431 1564 2023 2232 1431
Size: 133 459 209 3047
Fragments arranged by size:
3047 459 209 133
TatI w'GTAC_w
Cuts at: 655 3363 655
Size: 2708 1140
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 3816
Tth111I GACn'n_nGTC
Cuts at: 1645 1645
Size: 3848
XbaI T'CTAG_A
Cuts at: 879 879
Size: 3848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1343 1343
Size: 3848
XmaI C'CCGG_G
Cuts at: 868 868
Size: 3848
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI