Restriction map for
pF1KB5541
(Circular) (Six-base) MAPSORT of: pF1KB5541.seq Check: 7859 from: 1 to: 6044
pF1KB5541 6044 bp
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AatII G_ACGT'C
Cuts at: 755 1127 2516 755
Size: 372 1389 4283
Fragments arranged by size:
4283 1389 372
AccI GT'mk_AC
Cuts at: 355 1222 3082 355
Size: 867 1860 3317
Fragments arranged by size:
3317 1860 867
Acc65I G'GTAC_C
Cuts at: 3060 3060
Size: 6044
AclI AA'CG_TT
Cuts at: 5884 5884
Size: 6044
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1322 3869 4301 4654 5316 1322
Size: 2547 432 353 662 2050
Fragments arranged by size:
2547 2050 662 432 353
AflII C'TTAA_G
Cuts at: 1429 1429
Size: 6044
AflIII A'CryG_T
Cuts at: 306 1643 3396 5196 5477 306
Size: 1337 1753 1800 281 873
Fragments arranged by size:
1800 1753 1337 873 281
AleI CACnn'nnGTG
Cuts at: 1861 1861
Size: 6044
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 447 1480 2458 447
Size: 1033 978 4033
Fragments arranged by size:
4033 1033 978
AlwNI CAG_nnn'CTG
Cuts at: 1572 1593 3349 4787 1572
Size: 21 1756 1438 2829
Fragments arranged by size:
2829 1756 1438 21
ApaI G_GGCC'C
Cuts at: 1064 1064
Size: 6044
ApaLI G'TGCA_C
Cuts at: 4882 4882
Size: 6044
ApoI r'AATT_y
Cuts at: 1811 2770 3048 5397 5653 1811
Size: 959 278 2349 256 2202
Fragments arranged by size:
2349 2202 959 278 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6044
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6044
AvaI C'yCGr_G
Cuts at: 157 617 1364 1405 1668 1915 2127 3064 157
Size: 460 747 41 263 247 212 937 3137
Fragments arranged by size:
3137 937 747 460 263 247 212 41
BamHI G'GATC_C
Cuts at: 761 1907 2553 3069 761
Size: 1146 646 516 3736
Fragments arranged by size:
3736 1146 646 516
BanI G'GyrC_C
Cuts at: 1672 2298 2470 3060 3474 3722 3757 1672
Size: 626 172 590 414 248 35 3959
Fragments arranged by size:
3959 626 590 414 248 172 35
BanII G_rGCy'C
Cuts at: 626 1064 1637 1669 2102 3002 3011 3058 4088
Size: 438 573 32 433 900 9 47 1030
Cuts at: 4088 626
Size: 2582
Fragments arranged by size:
2582 1030 900 573 438 433 47 32
9
BbeI G_GCGC'C
Cuts at: 3478 3726 3478
Size: 248 5796
BbsI GAAGACnn'nnnn_
Cuts at: 2348 2516 2348
Size: 168 5876
BbvCI CC'TCA_GC
Cuts at: 1850 2571 1850
Size: 721 5323
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1000 1034 2023 2057 2565 2599 90
Size: 34 876 34 989 34 508 34 3535
Fragments arranged by size:
3535 989 876 508 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3942 4998 6044 3942
Size: 1056 1046 3942
Fragments arranged by size:
3942 1056 1046
BclI T'GATC_A
Cuts at: 1106 3564 1106
Size: 2458 3586
BfrBI ATG'CAT
Cuts at: 1512 1512
Size: 6044
BglI GCCn_nnn'nGGC
Cuts at: 112 117 1741 112
Size: 5 1624 4415
Fragments arranged by size:
4415 1624 5
BglII A'GATC_T
Cuts at: 2802 3559 5596 2802
Size: 757 2037 3250
Fragments arranged by size:
3250 2037 757
BlpI GC'TnA_GC
Cuts at: 2335 3156 2335
Size: 821 5223
Bme1580I G_kGCm'C
Cuts at: 1031 1064 1675 1858 3669 3762 4886 1031
Size: 33 611 183 1811 93 1124 2189
Fragments arranged by size:
2189 1811 1124 611 183 93 33
BmgBI CAC'GTC
Cuts at: 678 1646 1691 1893 2640 2909 678
Size: 968 45 202 747 269 3813
Fragments arranged by size:
3813 968 747 269 202 45
BmrI ACTGGGnnnn_n'
Cuts at: 3013 3256 3337 3672 5816 3013
Size: 243 81 335 2144 3241
Fragments arranged by size:
3241 2144 335 243 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2824 4411 2824
Size: 1587 4457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1255 1513 1576 1631 2116 2309 2608 2625 2737
Size: 258 63 55 485 193 299 17 112
Cuts at: 2737 3011 1255
Size: 274 4288
Fragments arranged by size:
4288 485 299 274 258 193 112 63
55 17
Bpu10I CC'TnA_GC
Cuts at: 1453 1504 1622 1850 2571 1453
Size: 51 118 228 721 4926
Fragments arranged by size:
4926 721 228 118 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1191 3220 4099 4813 5111 1191
Size: 2029 879 714 298 2124
Fragments arranged by size:
2124 2029 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 844 2162 5748 844
Size: 1318 3586 1140
Fragments arranged by size:
3586 1318 1140
BsaAI yAC'GTr
Cuts at: 2406 2524 2583 3397 2406
Size: 118 59 814 5053
Fragments arranged by size:
5053 814 118 59
BsaBI GATnn'nnATC
Cuts at: 6 832 3558 5595 6
Size: 826 2726 2037 455
Fragments arranged by size:
2726 2037 826 455
BsaHI Gr'CG_yC
Cuts at: 752 949 1076 1124 2513 3032 3475 3723 4425
Size: 197 127 48 1389 519 443 248 702
Cuts at: 4425 5921 752
Size: 1496 875
Fragments arranged by size:
1496 1389 875 702 519 443 248 197
127 48
BsaWI w'CCGG_w
Cuts at: 502 626 3233 3754 4432 4843 4990 5264 502
Size: 124 2607 521 678 411 147 274 1282
Fragments arranged by size:
2607 1282 678 521 411 274 147 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 760 790 2131 2161 760
Size: 30 1341 30 4643
Fragments arranged by size:
4643 1341 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1390 2797 2917 1390
Size: 1407 120 4517
Fragments arranged by size:
4517 1407 120
BseYI C'CCAG_C
Cuts at: 1114 1475 1684 2871 3011 3469 4227 4892 5300
Size: 361 209 1187 140 458 758 665 408
Cuts at: 5300 1114
Size: 1858
Fragments arranged by size:
1858 1187 758 665 458 408 361 209
140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1980 2063 2979 439
Size: 1541 83 916 3504
Fragments arranged by size:
3504 1541 916 83
BsiEI CG_ry'CG
Cuts at: 104 2681 3586 3632 4862 104
Size: 2577 905 46 1230 1286
Fragments arranged by size:
2577 1286 1230 905 46
BsiHKAI G_wGCw'C
Cuts at: 626 1459 1637 1669 1973 2588 3058 3836 4026
Size: 833 178 32 304 615 470 778 190
Cuts at: 4026 4886 626
Size: 860 1784
Fragments arranged by size:
1784 860 833 778 615 470 304 190
178 32
BsmI GAATG_Cn'
Cuts at: 639 639
Size: 6044
BsmBI CGTCTCn'nnnn_
Cuts at: 2860 2915 2860
Size: 55 5989
Bsp1286I G_dGCh'C
Cuts at: 626 1031 1064 1459 1637 1669 1675 1858 1973
Size: 405 33 395 178 32 6 183 115
Cuts at: 1973 2102 2588 3002 3011 3058 3669 3762 3836
Size: 129 486 414 9 47 611 93 74
Cuts at: 3836 4026 4088 4886 626
Size: 190 62 798 1784
Fragments arranged by size:
1784 798 611 486 414 405 395 190
183 178 129 115 93 74 62 47
33 32 9 6
BspHI T'CATG_A
Cuts at: 4476 4476
Size: 6044
BspMI ACCTGCnnnn'nnnn_
Cuts at: 285 2771 3094 3308 3610 3991 285
Size: 2486 323 214 302 381 2338
Fragments arranged by size:
2486 2338 381 323 302 214
BsrBI CCG'CTC
Cuts at: 50 4336 4390 5874 6039 50
Size: 4286 54 1484 165 55
Fragments arranged by size:
4286 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2024 2516 3956 2024
Size: 492 1440 4112
Fragments arranged by size:
4112 1440 492
BsrFI r'CCGG_y
Cuts at: 111 2009 4042 4223 111
Size: 1898 2033 181 1932
Fragments arranged by size:
2033 1932 1898 181
BssSI C'ACGA_G
Cuts at: 1009 2965 2995 4315 5023 1009
Size: 1956 30 1320 708 2030
Fragments arranged by size:
2030 1956 1320 708 30
BstAPI GCAn_nnn'nTGC
Cuts at: 1593 3421 1593
Size: 1828 4216
BstBI TT'CG_AA
Cuts at: 4405 4405
Size: 6044
BstXI CCAn_nnnn'nTGG
Cuts at: 1072 1233 1609 2431 1072
Size: 161 376 822 4685
Fragments arranged by size:
4685 822 376 161
BstYI r'GATC_y
Cuts at: 1 282 333 761 1562 1907 2553 2802 3069
Size: 281 51 428 801 345 646 249 267
Cuts at: 3069 3536 3559 3894 4140 4446 4458 4544 5596
Size: 467 23 335 246 306 12 86 1052
Cuts at: 5596 1
Size: 449
Fragments arranged by size:
1052 801 646 467 449 428 345 335
306 281 267 249 246 86 51 23
12
BstZ17I GTA'TAC
Cuts at: 356 356
Size: 6044
Bsu36I CC'TnA_GG
Cuts at: 668 668
Size: 6044
BtgI C'CryG_G
Cuts at: 1032 1415 1842 2711 2948 1032
Size: 383 427 869 237 4128
Fragments arranged by size:
4128 869 427 383 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2563 3994 4175 4338 2563
Size: 1431 181 163 4269
Fragments arranged by size:
4269 1431 181 163
BtsI GCAGTG_nn'
Cuts at: 661 2270 3287 661
Size: 1609 1017 3418
Fragments arranged by size:
3418 1609 1017
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 290 325 627 662 290
Size: 35 302 35 5672
Fragments arranged by size:
5672 302 35 35
DraI TTT'AAA
Cuts at: 275 3043 275
Size: 2768 3276
DraIII CAC_nnn'GTG
Cuts at: 702 902 702
Size: 200 5844
DrdI GACnn_nn'nnGTC
Cuts at: 873 1942 2874 3750 5094 873
Size: 1069 932 876 1344 1823
Fragments arranged by size:
1823 1344 1069 932 876
EaeI y'GGCC_r
Cuts at: 1895 3629 3803 4194 4221 5566 1895
Size: 1734 174 391 27 1345 2373
Fragments arranged by size:
2373 1734 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3629 3629
Size: 6044
EarI CTCTTCn'nnn_
Cuts at: 308 569 1897 2980 4067 4277 308
Size: 261 1328 1083 1087 210 2075
Fragments arranged by size:
2075 1328 1087 1083 261 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4252 4994 5140 5854 4252
Size: 742 146 714 4442
Fragments arranged by size:
4442 742 714 146
EcoICRI GAG'CTC
Cuts at: 624 1635 1667 3056 624
Size: 1011 32 1389 3612
Fragments arranged by size:
3612 1389 1011 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1255 1322 1513 1576 1631 2116 2309 2608 2625
Size: 67 191 63 55 485 193 299 17
Cuts at: 2625 2737 3011 3869 4301 4654 5316 1255
Size: 112 274 858 432 353 662 1983
Fragments arranged by size:
1983 858 662 485 432 353 299 274
193 191 112 67 63 55 17
EcoO109I rG'GnC_Cy
Cuts at: 297 1060 1061 1410 1700 2777 2876 3183 297
Size: 763 1 349 290 1077 99 307 3158
Fragments arranged by size:
3158 1077 763 349 307 290 99 1
EcoRI G'AATT_C
Cuts at: 3048 3048
Size: 6044
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4063 4063
Size: 6044
FspI TGC'GCA
Cuts at: 2084 3825 2084
Size: 1741 4303
HaeII r_GCGC'y
Cuts at: 3478 3726 4956 5727 3478
Size: 248 1230 771 3795
Fragments arranged by size:
3795 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 727 759 939 971 1497 1529 2541 2573 3886
Size: 32 180 32 526 32 1012 32 1313
Cuts at: 3886 3916 3918 3948 727
Size: 30 2 30 2823
Fragments arranged by size:
2823 1313 1012 526 180 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 215 1329 3083 215
Size: 1114 1754 3176
Fragments arranged by size:
3176 1754 1114
KasI G'GCGC_C
Cuts at: 3474 3722 3474
Size: 248 5796
KpnI G_GTAC'C
Cuts at: 3064 3064
Size: 6044
MfeI C'AATT_G
Cuts at: 1859 1859
Size: 6044
MluI A'CGCG_T
Cuts at: 5477 5477
Size: 6044
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1730 2261 4803 4987 1730
Size: 531 2542 184 2787
Fragments arranged by size:
2787 2542 531 184
MscI TGG'CCA
Cuts at: 3805 3805
Size: 6044
MslI CAynn'nnrTG
Cuts at: 429 598 836 1861 2197 4033 4160 429
Size: 169 238 1025 336 1836 127 2313
Fragments arranged by size:
2313 1836 1025 336 238 169 127
MspA1I CmG'CkG
Cuts at: 807 1034 1593 1950 2448 3155 3469 3829 4613
Size: 227 559 357 498 707 314 360 784
Cuts at: 4613 4858 807
Size: 245 1993
Fragments arranged by size:
1993 784 707 559 498 360 357 314
245 227
NaeI GCC'GGC
Cuts at: 113 4225 113
Size: 4112 1932
NarI GG'CG_CC
Cuts at: 3475 3723 3475
Size: 248 5796
NcoI C'CATG_G
Cuts at: 1842 2948 1842
Size: 1106 4938
NgoMIV G'CCGG_C
Cuts at: 111 4223 111
Size: 4112 1932
NsiI A_TGCA'T
Cuts at: 1514 1514
Size: 6044
NspI r_CATG'y
Cuts at: 238 310 1512 1878 3097 3597 5200 238
Size: 72 1202 366 1219 500 1603 1082
Fragments arranged by size:
1603 1219 1202 1082 500 366 72
PasI CC'CwG_GG
Cuts at: 1697 1697
Size: 6044
PciI A'CATG_T
Cuts at: 306 5196 306
Size: 4890 1154
PfoI T'CCnGG_A
Cuts at: 1137 1637 2633 1137
Size: 500 996 4548
Fragments arranged by size:
4548 996 500
PmeI GTTT'AAAC
Cuts at: 3043 3043
Size: 6044
PmlI CAC'GTG
Cuts at: 2524 2583 2524
Size: 59 5985
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4490 4522 4490
Size: 32 6012
PpuMI rG'GwC_Cy
Cuts at: 297 2777 2876 297
Size: 2480 99 3465
Fragments arranged by size:
3465 2480 99
PspOMI G'GGCC_C
Cuts at: 1060 1060
Size: 6044
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2347 2379 2347
Size: 32 6012
PstI C_TGCA'G
Cuts at: 296 1577 2117 2388 2447 2479 2856 3091 3776
Size: 1281 540 271 59 32 377 235 685
Cuts at: 3776 296
Size: 2564
Fragments arranged by size:
2564 1281 685 540 377 271 235 59
32
PvuI CG_AT'CG
Cuts at: 104 2681 104
Size: 2577 3467
PvuII CAG'CTG
Cuts at: 1593 1950 3469 3829 1593
Size: 357 1519 360 3808
Fragments arranged by size:
3808 1519 360 357
RsrII CG'GwC_CG
Cuts at: 4239 4239
Size: 6044
SacI G_AGCT'C
Cuts at: 626 1637 1669 3058 626
Size: 1011 32 1389 3612
Fragments arranged by size:
3612 1389 1011 32
SacII CC_GC'GG
Cuts at: 1035 1035
Size: 6044
SalI G'TCGA_C
Cuts at: 3081 3081
Size: 6044
SanDI GG'GwC_CC
Cuts at: 2876 2876
Size: 6044
SapI GCTCTTCn'nnn_
Cuts at: 308 4067 4277 308
Size: 3759 210 2075
Fragments arranged by size:
3759 2075 210
SbfI CC_TGCA'GG
Cuts at: 296 3091 296
Size: 2795 3249
ScaI AGT'ACT
Cuts at: 5561 5561
Size: 6044
SexAI A'CCwGG_T
Cuts at: 1322 1322
Size: 6044
SfcI C'TryA_G
Cuts at: 33 292 1573 2113 2384 2443 2475 2852 3087
Size: 259 1281 540 271 59 32 377 235
Cuts at: 3087 3772 4740 4931 33
Size: 685 968 191 1146
Fragments arranged by size:
1281 1146 968 685 540 377 271 259
235 191 59 32
SfoI GGC'GCC
Cuts at: 3476 3724 3476
Size: 248 5796
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6044
SmaI CCC'GGG
Cuts at: 3066 3066
Size: 6044
SmlI C'TyrA_G
Cuts at: 1170 1429 3199 4114 4828 5090 1170
Size: 259 1770 915 714 262 2124
Fragments arranged by size:
2124 1770 915 714 262 259
SnaBI TAC'GTA
Cuts at: 2406 2406
Size: 6044
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6044
SphI G_CATG'C
Cuts at: 238 1512 3097 3597 238
Size: 1274 1585 500 2685
Fragments arranged by size:
2685 1585 1274 500
StuI AGG'CCT
Cuts at: 383 383
Size: 6044
StyI C'CwwG_G
Cuts at: 920 1739 1826 1842 2948 3178 3532 920
Size: 819 87 16 1106 230 354 3432
Fragments arranged by size:
3432 1106 819 354 230 87 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1467 1619 2458 2510 3627 3760 4219 4428 1467
Size: 152 839 52 1117 133 459 209 3083
Fragments arranged by size:
3083 1117 839 459 209 152 133 52
TatI w'GTAC_w
Cuts at: 2021 5559 2021
Size: 3538 2506
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 688 720 742 774 2131 2163 2171 2203 5561
Size: 32 22 32 1357 32 8 32 3358
Cuts at: 5561 5593 688
Size: 32 1139
Fragments arranged by size:
3358 1357 1139 32 32 32 32 32
22 8
Tth111I GACn'n_nGTC
Cuts at: 522 3841 522
Size: 3319 2725
XbaI T'CTAG_A
Cuts at: 3075 3075
Size: 6044
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 411 1833 2130 2463 3539 411
Size: 1422 297 333 1076 2916
Fragments arranged by size:
2916 1422 1076 333 297
XmaI C'CCGG_G
Cuts at: 3064 3064
Size: 6044
ZraI GAC'GTC
Cuts at: 753 1125 2514 753
Size: 372 1389 4283
Fragments arranged by size:
4283 1389 372
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AgeI AhdI AloI AscI AvrII BaeI
BmtI BsiWI BspEI BsrGI BssHII BstEII ClaI EcoNI
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI PacI PflMI PshAI PsiI PspXI SfiI SgrAI
SrfI SspI SwaI XhoI XmnI