Restriction map for
pF1KB5539
(Circular) (Six-base) MAPSORT of: pF1KB5539.seq Check: 6659 from: 1 to: 4796
pF1KB5539 4796 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 112 1037 1834 112
Size: 925 797 3074
Fragments arranged by size:
3074 925 797
Acc65I G'GTAC_C
Cuts at: 1812 1812
Size: 4796
AclI AA'CG_TT
Cuts at: 4636 4636
Size: 4796
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 974 990 1175 1324 1616 2621 3053 3800 4068
Size: 16 185 149 292 1005 432 747 268
Cuts at: 4068 974
Size: 1702
Fragments arranged by size:
1702 1005 747 432 292 268 185 149
16
AflIII A'CryG_T
Cuts at: 1628 2148 3252 4229 1628
Size: 520 1104 977 2195
Fragments arranged by size:
2195 1104 977 520
AgeI A'CCGG_T
Cuts at: 3184 3184
Size: 4796
AlwNI CAG_nnn'CTG
Cuts at: 462 484 1744 2101 3668 462
Size: 22 1260 357 1567 1590
Fragments arranged by size:
1590 1567 1260 357 22
ApaLI G'TGCA_C
Cuts at: 3566 3566
Size: 4796
ApoI r'AATT_y
Cuts at: 1800 4149 4405 1800
Size: 2349 256 2191
Fragments arranged by size:
2349 2191 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4796
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
AvaI C'yCGr_G
Cuts at: 1226 1816 1226
Size: 590 4206
BamHI G'GATC_C
Cuts at: 173 1073 1821 173
Size: 900 748 3148
Fragments arranged by size:
3148 900 748
BanI G'GyrC_C
Cuts at: 1237 1812 2226 2474 2509 1237
Size: 575 414 248 35 3524
Fragments arranged by size:
3524 575 414 248 35
BanII G_rGCy'C
Cuts at: 1227 1320 1699 1810 2840 1227
Size: 93 379 111 1030 3183
Fragments arranged by size:
3183 1030 379 111 93
BbeI G_GCGC'C
Cuts at: 2230 2478 2230
Size: 248 4548
BbsI GAAGACnn'nnnn_
Cuts at: 782 1163 1604 782
Size: 381 441 3974
Fragments arranged by size:
3974 441 381
BbvCI CC'TCA_GC
Cuts at: 1729 1729
Size: 4796
BciVI GTATCCnnnnn_n'
Cuts at: 1570 2694 3455 4796 1570
Size: 1124 761 1341 1570
Fragments arranged by size:
1570 1341 1124 761
BclI T'GATC_A
Cuts at: 2316 2316
Size: 4796
BglII A'GATC_T
Cuts at: 508 1469 1511 2311 4348 508
Size: 961 42 800 2037 956
Fragments arranged by size:
2037 961 956 800 42
BlpI GC'TnA_GC
Cuts at: 1908 1908
Size: 4796
Bme1580I G_kGCm'C
Cuts at: 2421 2514 3570 2421
Size: 93 1056 3647
Fragments arranged by size:
3647 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 472 989 1499 2008 2089 2424 4568 472
Size: 517 510 509 81 335 2144 700
Fragments arranged by size:
2144 700 517 510 509 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 737 3163 737
Size: 2426 2370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 700 821 1721 590
Size: 110 121 900 3665
Fragments arranged by size:
3665 900 121 110
Bpu10I CC'TnA_GC
Cuts at: 1336 1729 1336
Size: 393 4403
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1972 2851 3343 3641 1972
Size: 879 492 298 3127
Fragments arranged by size:
3127 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1678 4500 1678
Size: 2822 1974
BsaAI yAC'GTr
Cuts at: 2149 2149
Size: 4796
BsaBI GATnn'nnATC
Cuts at: 6 1078 2310 4347 6
Size: 1072 1232 2037 455
Fragments arranged by size:
2037 1232 1072 455
BsaHI Gr'CG_yC
Cuts at: 2227 2475 3177 4673 2227
Size: 248 702 1496 2350
Fragments arranged by size:
2350 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1985 2506 3184 3458 3605 4016 1985
Size: 521 678 274 147 411 2765
Fragments arranged by size:
2765 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1169 1199 1169
Size: 30 4766
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 854 893 854
Size: 39 4757
BseYI C'CCAG_C
Cuts at: 177 1130 2221 2979 3556 4052 177
Size: 953 1091 758 577 496 921
Fragments arranged by size:
1091 953 921 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 833 833
Size: 4796
BsiEI CG_ry'CG
Cuts at: 104 2338 2384 3592 104
Size: 2234 46 1208 1308
Fragments arranged by size:
2234 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 360 1058 1699 1810 2588 2778 3570 360
Size: 698 641 111 778 190 792 1586
Fragments arranged by size:
1586 792 778 698 641 190 111
Bsp1286I G_dGCh'C
Cuts at: 360 1058 1227 1320 1699 1810 2421 2514 2588
Size: 698 169 93 379 111 611 93 74
Cuts at: 2588 2778 2840 3570 360
Size: 190 62 730 1586
Fragments arranged by size:
1586 730 698 611 379 190 169 111
93 93 74 62
BspEI T'CCGG_A
Cuts at: 4016 4016
Size: 4796
BspHI T'CATG_A
Cuts at: 3972 3972
Size: 4796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 203 1846 2060 2362 2743 203
Size: 1643 214 302 381 2256
Fragments arranged by size:
2256 1643 381 302 214
BsrBI CCG'CTC
Cuts at: 50 237 3088 3142 4626 4791 50
Size: 187 2851 54 1484 165 55
Fragments arranged by size:
2851 1484 187 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1539 2708 1539
Size: 1169 3627
BsrFI r'CCGG_y
Cuts at: 314 2794 2975 3184 314
Size: 2480 181 209 1926
Fragments arranged by size:
2480 1926 209 181
BssSI C'ACGA_G
Cuts at: 3067 3425 3067
Size: 358 4438
BstAPI GCAn_nnn'nTGC
Cuts at: 193 1378 2173 193
Size: 1185 795 2816
Fragments arranged by size:
2816 1185 795
BstBI TT'CG_AA
Cuts at: 3157 3157
Size: 4796
BstEII G'GTnAC_C
Cuts at: 1390 1390
Size: 4796
BstXI CCAn_nnnn'nTGG
Cuts at: 815 1084 815
Size: 269 4527
BstYI r'GATC_y
Cuts at: 1 173 458 508 1073 1469 1493 1511 1821
Size: 172 285 50 565 396 24 18 310
Cuts at: 1821 2288 2311 2646 2892 3904 3990 4002 4348
Size: 467 23 335 246 1012 86 12 346
Cuts at: 4348 1
Size: 449
Fragments arranged by size:
1012 565 467 449 396 346 335 310
285 246 172 86 50 24 23 18
12
Bsu36I CC'TnA_GG
Cuts at: 1312 1386 1312
Size: 74 4722
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 404 2746 2927 3090 404
Size: 2342 181 163 2110
Fragments arranged by size:
2342 2110 181 163
BtsI GCAGTG_nn'
Cuts at: 2039 2039
Size: 4796
DraI TTT'AAA
Cuts at: 1795 1795
Size: 4796
DrdI GACnn_nn'nnGTC
Cuts at: 138 2502 3360 138
Size: 2364 858 1574
Fragments arranged by size:
2364 1574 858
EaeI y'GGCC_r
Cuts at: 312 1504 1643 2381 2555 2946 2973 4318 312
Size: 1192 139 738 174 391 27 1345 790
Fragments arranged by size:
1345 1192 790 738 391 174 139 27
EagI C'GGCC_G
Cuts at: 2381 2381
Size: 4796
EarI CTCTTCn'nnn_
Cuts at: 227 706 765 1397 2819 3029 227
Size: 479 59 632 1422 210 1994
Fragments arranged by size:
1994 1422 632 479 210 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3004 3314 3460 4606 3004
Size: 310 146 1146 3194
Fragments arranged by size:
3194 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1697 1808 1697
Size: 111 4685
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 700 821 974 990 1175 1324 1616 1721
Size: 110 121 153 16 185 149 292 105
Cuts at: 1721 2621 3053 3800 4068 590
Size: 900 432 747 268 1318
Fragments arranged by size:
1318 900 747 432 292 268 185 153
149 121 110 105 16
EcoO109I rG'GnC_Cy
Cuts at: 741 1230 1308 1935 741
Size: 489 78 627 3602
Fragments arranged by size:
3602 627 489 78
EcoRI G'AATT_C
Cuts at: 1800 1800
Size: 4796
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1533 2815 1533
Size: 1282 3514
FspI TGC'GCA
Cuts at: 2577 2577
Size: 4796
HaeII r_GCGC'y
Cuts at: 2230 2478 3500 4479 2230
Size: 248 1022 979 2547
Fragments arranged by size:
2547 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 342 374 580 612 1214 1246 2638 2668 2670
Size: 32 206 32 602 32 1392 30 2
Cuts at: 2670 2700 342
Size: 30 2438
Fragments arranged by size:
2438 1392 602 206 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1275 1835 1275
Size: 560 4236
HindIII A'AGCT_T
Cuts at: 1111 1409 1111
Size: 298 4498
KasI G'GCGC_C
Cuts at: 2226 2474 2226
Size: 248 4548
KpnI G_GTAC'C
Cuts at: 1816 1816
Size: 4796
MfeI C'AATT_G
Cuts at: 1102 1583 1102
Size: 481 4315
MluI A'CGCG_T
Cuts at: 4229 4229
Size: 4796
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3467 3651 3467
Size: 184 4612
MscI TGG'CCA
Cuts at: 1506 1645 2557 1506
Size: 139 912 3745
Fragments arranged by size:
3745 912 139
MslI CAynn'nnrTG
Cuts at: 892 1019 2785 2912 892
Size: 127 1766 127 2776
Fragments arranged by size:
2776 1766 127 127
MspA1I CmG'CkG
Cuts at: 339 1907 2221 2581 3594 3839 339
Size: 1568 314 360 1013 245 1296
Fragments arranged by size:
1568 1296 1013 360 314 245
NaeI GCC'GGC
Cuts at: 316 2977 316
Size: 2661 2135
NarI GG'CG_CC
Cuts at: 2227 2475 2227
Size: 248 4548
NgoMIV G'CCGG_C
Cuts at: 314 2975 314
Size: 2661 2135
NspI r_CATG'y
Cuts at: 1174 1632 1849 2349 3256 1174
Size: 458 217 500 907 2714
Fragments arranged by size:
2714 907 500 458 217
PasI CC'CwG_GG
Cuts at: 162 162
Size: 4796
PciI A'CATG_T
Cuts at: 1628 3252 1628
Size: 1624 3172
PmeI GTTT'AAAC
Cuts at: 1795 1795
Size: 4796
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 3935
Size: 32 4764
PpuMI rG'GwC_Cy
Cuts at: 741 1230 1308 741
Size: 489 78 4229
Fragments arranged by size:
4229 489 78
PsiI TTA'TAA
Cuts at: 628 628
Size: 4796
PstI C_TGCA'G
Cuts at: 732 1843 2528 732
Size: 1111 685 3000
Fragments arranged by size:
3000 1111 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4796
PvuII CAG'CTG
Cuts at: 339 2221 2581 339
Size: 1882 360 2554
Fragments arranged by size:
2554 1882 360
RsrII CG'GwC_CG
Cuts at: 2991 2991
Size: 4796
SacI G_AGCT'C
Cuts at: 1699 1810 1699
Size: 111 4685
SalI G'TCGA_C
Cuts at: 1833 1833
Size: 4796
SanDI GG'GwC_CC
Cuts at: 741 741
Size: 4796
SapI GCTCTTCn'nnn_
Cuts at: 227 2819 3029 227
Size: 2592 210 1994
Fragments arranged by size:
2592 1994 210
SbfI CC_TGCA'GG
Cuts at: 1843 1843
Size: 4796
ScaI AGT'ACT
Cuts at: 4313 4313
Size: 4796
SfcI C'TryA_G
Cuts at: 33 728 1839 2524 3517 3708 33
Size: 695 1111 685 993 191 1121
Fragments arranged by size:
1121 1111 993 695 685 191
SfoI GGC'GCC
Cuts at: 2228 2476 2228
Size: 248 4548
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
SmaI CCC'GGG
Cuts at: 1228 1818 1228
Size: 590 4206
SmlI C'TyrA_G
Cuts at: 1951 2866 3358 3620 1951
Size: 915 492 262 3127
Fragments arranged by size:
3127 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4796
SphI G_CATG'C
Cuts at: 1849 2349 1849
Size: 500 4296
SspI AAT'ATT
Cuts at: 330 330
Size: 4796
StyI C'CwwG_G
Cuts at: 168 1241 1930 2284 168
Size: 1073 689 354 2680
Fragments arranged by size:
2680 1073 689 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 885 2379 2512 2971 3180 885
Size: 1494 133 459 209 2501
Fragments arranged by size:
2501 1494 459 209 133
TatI w'GTAC_w
Cuts at: 1566 4311 1566
Size: 2745 2051
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4313 4345 4313
Size: 32 4764
Tth111I GACn'n_nGTC
Cuts at: 2593 2593
Size: 4796
XbaI T'CTAG_A
Cuts at: 1827 1827
Size: 4796
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 834 2291 834
Size: 1457 3339
XmaI C'CCGG_G
Cuts at: 1226 1816 1226
Size: 590 4206
XmnI GAAnn'nnTTC
Cuts at: 704 704
Size: 4796
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PshAI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI