Restriction map for
pF1KB5535
(Circular) (Six-base) MAPSORT of: pF1KB5535.seq Check: 5784 from: 1 to: 4502
pF1KB5535 4502 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 195 195
Size: 4502
AccI GT'mk_AC
Cuts at: 924 1540 924
Size: 616 3886
Acc65I G'GTAC_C
Cuts at: 1518 1518
Size: 4502
AclI AA'CG_TT
Cuts at: 4342 4342
Size: 4502
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 2327 2759 3506 3774 295
Size: 2032 432 747 268 1023
Fragments arranged by size:
2032 1023 747 432 268
AflII C'TTAA_G
Cuts at: 574 574
Size: 4502
AflIII A'CryG_T
Cuts at: 1854 2958 3935 1854
Size: 1104 977 2421
Fragments arranged by size:
2421 1104 977
AgeI A'CCGG_T
Cuts at: 2890 2890
Size: 4502
AlwNI CAG_nnn'CTG
Cuts at: 318 1087 1360 1807 3374 318
Size: 769 273 447 1567 1446
Fragments arranged by size:
1567 1446 769 447 273
ApaI G_GGCC'C
Cuts at: 122 1214 122
Size: 1092 3410
ApaLI G'TGCA_C
Cuts at: 3272 3272
Size: 4502
ApoI r'AATT_y
Cuts at: 579 1506 3855 4111 579
Size: 927 2349 256 970
Fragments arranged by size:
2349 970 927 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4502
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
AvaI C'yCGr_G
Cuts at: 1522 1522
Size: 4502
BamHI G'GATC_C
Cuts at: 1427 1527 1427
Size: 100 4402
BanI G'GyrC_C
Cuts at: 166 217 1396 1518 1932 2180 2215 166
Size: 51 1179 122 414 248 35 2453
Fragments arranged by size:
2453 1179 414 248 122 51 35
BanII G_rGCy'C
Cuts at: 122 241 1214 1516 2546 122
Size: 119 973 302 1030 2078
Fragments arranged by size:
2078 1030 973 302 119
BbeI G_GCGC'C
Cuts at: 170 1936 2184 170
Size: 1766 248 2488
Fragments arranged by size:
2488 1766 248
BbsI GAAGACnn'nnnn_
Cuts at: 219 1118 219
Size: 899 3603
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 169 203 169
Size: 34 4468
BciVI GTATCCnnnnn_n'
Cuts at: 315 2400 3161 4502 315
Size: 2085 761 1341 315
Fragments arranged by size:
2085 1341 761 315
BclI T'GATC_A
Cuts at: 618 2022 618
Size: 1404 3098
BglII A'GATC_T
Cuts at: 2017 4054 2017
Size: 2037 2465
BlpI GC'TnA_GC
Cuts at: 1614 1614
Size: 4502
Bme1580I G_kGCm'C
Cuts at: 122 220 1214 1399 2127 2220 3276 122
Size: 98 994 185 728 93 1056 1348
Fragments arranged by size:
1348 1056 994 728 185 98 93
BmrI ACTGGGnnnn_n'
Cuts at: 485 516 1714 1795 2130 4274 485
Size: 31 1198 81 335 2144 713
Fragments arranged by size:
2144 1198 713 335 81 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2869 2869
Size: 4502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 837 225
Size: 612 3890
Bpu10I CC'TnA_GC
Cuts at: 955 955
Size: 4502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 977 1213 1678 2557 3049 3347 977
Size: 236 465 879 492 298 2132
Fragments arranged by size:
2132 879 492 465 298 236
BsaI GGTCTCn'nnnn_
Cuts at: 1283 4206 1283
Size: 2923 1579
BsaAI yAC'GTr
Cuts at: 1855 1855
Size: 4502
BsaBI GATnn'nnATC
Cuts at: 6 2016 4053 6
Size: 2010 2037 455
Fragments arranged by size:
2037 2010 455
BsaHI Gr'CG_yC
Cuts at: 167 1933 2181 2883 4379 167
Size: 1766 248 702 1496 290
Fragments arranged by size:
1766 1496 702 290 248
BsaWI w'CCGG_w
Cuts at: 520 1424 1691 2212 2890 3164 3311 3722 520
Size: 904 267 521 678 274 147 411 1300
Fragments arranged by size:
1300 904 678 521 411 274 267 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 708 738 958 988 1077 1107 708
Size: 30 220 30 89 30 4103
Fragments arranged by size:
4103 220 89 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1122 1122
Size: 4502
BseYI C'CCAG_C
Cuts at: 115 213 251 1391 1403 1927 2685 3262 3758
Size: 98 38 1140 12 524 758 577 496
Cuts at: 3758 115
Size: 859
Fragments arranged by size:
1140 859 758 577 524 496 98 38
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 743 180
Size: 563 3939
BsiEI CG_ry'CG
Cuts at: 104 2044 2090 3298 104
Size: 1940 46 1208 1308
Fragments arranged by size:
1940 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1516 2294 2484 3276 1516
Size: 778 190 792 2742
Fragments arranged by size:
2742 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 122 220 241 1214 1399 1516 2127 2220 2294
Size: 98 21 973 185 117 611 93 74
Cuts at: 2294 2484 2546 3276 122
Size: 190 62 730 1348
Fragments arranged by size:
1348 973 730 611 190 185 117 98
93 74 62 21
BspEI T'CCGG_A
Cuts at: 1424 3722 1424
Size: 2298 2204
BspHI T'CATG_A
Cuts at: 3678 3678
Size: 4502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 195 218 765 1552 1766 2068 2449 195
Size: 23 547 787 214 302 381 2248
Fragments arranged by size:
2248 787 547 381 302 214 23
BsrBI CCG'CTC
Cuts at: 50 2794 2848 4332 4497 50
Size: 2744 54 1484 165 55
Fragments arranged by size:
2744 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1179 2414 1179
Size: 1235 3267
BsrFI r'CCGG_y
Cuts at: 1399 2500 2681 2890 1399
Size: 1101 181 209 3011
Fragments arranged by size:
3011 1101 209 181
BssSI C'ACGA_G
Cuts at: 2773 3131 2773
Size: 358 4144
BstAPI GCAn_nnn'nTGC
Cuts at: 1879 1879
Size: 4502
BstBI TT'CG_AA
Cuts at: 855 2863 855
Size: 2008 2494
BstEII G'GTnAC_C
Cuts at: 447 447
Size: 4502
BstXI CCAn_nnnn'nTGG
Cuts at: 809 1048 1279 809
Size: 239 231 4032
Fragments arranged by size:
4032 239 231
BstYI r'GATC_y
Cuts at: 1 1265 1427 1527 1994 2017 2352 2598 3610
Size: 1264 162 100 467 23 335 246 1012
Cuts at: 3610 3696 3708 4054 1
Size: 86 12 346 449
Fragments arranged by size:
1264 1012 467 449 346 335 246 162
100 86 23 12
BstZ17I GTA'TAC
Cuts at: 925 925
Size: 4502
Bsu36I CC'TnA_GG
Cuts at: 962 962
Size: 4502
BtgI C'CryG_G
Cuts at: 107 1214 107
Size: 1107 3395
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 272 2452 2633 2796 272
Size: 2180 181 163 1978
Fragments arranged by size:
2180 1978 181 163
BtsI GCAGTG_nn'
Cuts at: 1475 1745 1475
Size: 270 4232
DraI TTT'AAA
Cuts at: 1501 1501
Size: 4502
DrdI GACnn_nn'nnGTC
Cuts at: 2208 3066 2208
Size: 858 3644
EaeI y'GGCC_r
Cuts at: 351 726 2087 2261 2652 2679 4024 351
Size: 375 1361 174 391 27 1345 829
Fragments arranged by size:
1361 1345 829 391 375 174 27
EagI C'GGCC_G
Cuts at: 2087 2087
Size: 4502
EarI CTCTTCn'nnn_
Cuts at: 428 2525 2735 428
Size: 2097 210 2195
Fragments arranged by size:
2195 2097 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2710 3020 3166 4312 2710
Size: 310 146 1146 2900
Fragments arranged by size:
2900 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1514 1514
Size: 4502
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 295 837 2327 2759 3506 3774 225
Size: 70 542 1490 432 747 268 953
Fragments arranged by size:
1490 953 747 542 432 268 70
EcoO109I rG'GnC_Cy
Cuts at: 119 1641 119
Size: 1522 2980
EcoRI G'AATT_C
Cuts at: 579 1506 579
Size: 927 3575
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1310 2521 1310
Size: 1211 3291
FspI TGC'GCA
Cuts at: 2283 2283
Size: 4502
HaeII r_GCGC'y
Cuts at: 170 1936 2184 3206 4185 170
Size: 1766 248 1022 979 487
Fragments arranged by size:
1766 1022 979 487 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 958 990 1342 1374 2344 2374 2376 2406 958
Size: 32 352 32 970 30 2 30 3054
Fragments arranged by size:
3054 970 352 32 32 30 30 2
HincII GTy'rAC
Cuts at: 264 561 1541 264
Size: 297 980 3225
Fragments arranged by size:
3225 980 297
HindIII A'AGCT_T
Cuts at: 1064 1189 1064
Size: 125 4377
KasI G'GCGC_C
Cuts at: 166 1932 2180 166
Size: 1766 248 2488
Fragments arranged by size:
2488 1766 248
KpnI G_GTAC'C
Cuts at: 1522 1522
Size: 4502
MfeI C'AATT_G
Cuts at: 1454 1454
Size: 4502
MluI A'CGCG_T
Cuts at: 3935 3935
Size: 4502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3173 3357 3173
Size: 184 4318
MscI TGG'CCA
Cuts at: 353 728 2263 353
Size: 375 1535 2592
Fragments arranged by size:
2592 1535 375
MslI CAynn'nnrTG
Cuts at: 1468 2491 2618 1468
Size: 1023 127 3352
Fragments arranged by size:
3352 1023 127
MspA1I CmG'CkG
Cuts at: 245 878 1391 1613 1927 2287 3300 3545 245
Size: 633 513 222 314 360 1013 245 1202
Fragments arranged by size:
1202 1013 633 513 360 314 245 222
NaeI GCC'GGC
Cuts at: 2683 2683
Size: 4502
NarI GG'CG_CC
Cuts at: 167 1933 2181 167
Size: 1766 248 2488
Fragments arranged by size:
2488 1766 248
NcoI C'CATG_G
Cuts at: 107 1214 107
Size: 1107 3395
NdeI CA'TA_TG
Cuts at: 330 330
Size: 4502
NgoMIV G'CCGG_C
Cuts at: 2681 2681
Size: 4502
NspI r_CATG'y
Cuts at: 1555 2055 2962 1555
Size: 500 907 3095
Fragments arranged by size:
3095 907 500
PasI CC'CwG_GG
Cuts at: 699 699
Size: 4502
PciI A'CATG_T
Cuts at: 2958 2958
Size: 4502
PmeI GTTT'AAAC
Cuts at: 1501 1501
Size: 4502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3641 3673 3641
Size: 32 4470
PspOMI G'GGCC_C
Cuts at: 118 1210 118
Size: 1092 3410
PstI C_TGCA'G
Cuts at: 1549 2234 1549
Size: 685 3817
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4502
PvuII CAG'CTG
Cuts at: 878 1391 1927 2287 878
Size: 513 536 360 3093
Fragments arranged by size:
3093 536 513 360
RsrII CG'GwC_CG
Cuts at: 2697 2697
Size: 4502
SacI G_AGCT'C
Cuts at: 1516 1516
Size: 4502
SalI G'TCGA_C
Cuts at: 1539 1539
Size: 4502
SapI GCTCTTCn'nnn_
Cuts at: 2525 2735 2525
Size: 210 4292
SbfI CC_TGCA'GG
Cuts at: 1549 1549
Size: 4502
ScaI AGT'ACT
Cuts at: 4019 4019
Size: 4502
SfcI C'TryA_G
Cuts at: 33 1545 2230 3223 3414 33
Size: 1512 685 993 191 1121
Fragments arranged by size:
1512 1121 993 685 191
SfoI GGC'GCC
Cuts at: 168 1934 2182 168
Size: 1766 248 2488
Fragments arranged by size:
2488 1766 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
SmaI CCC'GGG
Cuts at: 1524 1524
Size: 4502
SmlI C'TyrA_G
Cuts at: 574 992 1192 1657 2572 3064 3326 574
Size: 418 200 465 915 492 262 1750
Fragments arranged by size:
1750 915 492 465 418 262 200
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4502
SphI G_CATG'C
Cuts at: 1555 2055 1555
Size: 500 4002
StyI C'CwwG_G
Cuts at: 107 339 375 745 1214 1636 1990 107
Size: 232 36 370 469 422 354 2619
Fragments arranged by size:
2619 469 422 370 354 232 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 467 471 2085 2218 2677 2886 100
Size: 367 4 1614 133 459 209 1716
Fragments arranged by size:
1716 1614 459 367 209 133 4
TatI w'GTAC_w
Cuts at: 4017 4017
Size: 4502
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 515 547 708 740 1077 1109 1470 1502 4019
Size: 32 161 32 337 32 361 32 2517
Cuts at: 4019 4051 515
Size: 32 966
Fragments arranged by size:
2517 966 361 337 161 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2299 2299
Size: 4502
XbaI T'CTAG_A
Cuts at: 1533 1533
Size: 4502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 868 1997 868
Size: 1129 3373
XmaI C'CCGG_G
Cuts at: 1522 1522
Size: 4502
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI