Restriction map for
pF1KB5514
(Circular) (Six-base) MAPSORT of: pF1KB5514.seq Check: 3238 from: 1 to: 4454
pF1KB5514 4454 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 445 836 1492 445
Size: 391 656 3407
Fragments arranged by size:
3407 656 391
Acc65I G'GTAC_C
Cuts at: 1470 1470
Size: 4454
AclI AA'CG_TT
Cuts at: 4294 4294
Size: 4454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 944 1188 1303 2279 2711 3064 3726 944
Size: 244 115 976 432 353 662 1672
Fragments arranged by size:
1672 976 662 432 353 244 115
AflIII A'CryG_T
Cuts at: 1806 3606 3887 1806
Size: 1800 281 2373
Fragments arranged by size:
2373 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1023 1023
Size: 4454
AlwNI CAG_nnn'CTG
Cuts at: 118 1759 3197 118
Size: 1641 1438 1375
Fragments arranged by size:
1641 1438 1375
ApaLI G'TGCA_C
Cuts at: 3292 3292
Size: 4454
ApoI r'AATT_y
Cuts at: 211 805 982 1034 1141 1458 3807 4063 211
Size: 594 177 52 107 317 2349 256 602
Fragments arranged by size:
2349 602 594 317 256 177 107 52
AseI AT'TA_AT
Cuts at: 20 662 707 20
Size: 642 45 3767
Fragments arranged by size:
3767 642 45
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4454
AvaI C'yCGr_G
Cuts at: 1474 1474
Size: 4454
BamHI G'GATC_C
Cuts at: 1479 1479
Size: 4454
BanI G'GyrC_C
Cuts at: 1379 1470 1884 2132 2167 1379
Size: 91 414 248 35 3666
Fragments arranged by size:
3666 414 248 91 35
BanII G_rGCy'C
Cuts at: 1468 2498 1468
Size: 1030 3424
BbeI G_GCGC'C
Cuts at: 1888 2136 1888
Size: 248 4206
BciVI GTATCCnnnnn_n'
Cuts at: 2352 3408 4454 2352
Size: 1056 1046 2352
Fragments arranged by size:
2352 1056 1046
BclI T'GATC_A
Cuts at: 1974 1974
Size: 4454
BglII A'GATC_T
Cuts at: 1969 4006 1969
Size: 2037 2417
BlpI GC'TnA_GC
Cuts at: 1566 1566
Size: 4454
Bme1580I G_kGCm'C
Cuts at: 2079 2172 3296 2079
Size: 93 1124 3237
Fragments arranged by size:
3237 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 926 1666 1747 2082 4226 926
Size: 740 81 335 2144 1154
Fragments arranged by size:
2144 1154 740 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2821 2821
Size: 4454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 245 140
Size: 105 4349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1630 2509 3223 3521 1630
Size: 879 714 298 2563
Fragments arranged by size:
2563 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4158 4158
Size: 4454
BsaAI yAC'GTr
Cuts at: 1807 1807
Size: 4454
BsaBI GATnn'nnATC
Cuts at: 6 1968 4005 6
Size: 1962 2037 455
Fragments arranged by size:
2037 1962 455
BsaHI Gr'CG_yC
Cuts at: 1885 2133 2835 4331 1885
Size: 248 702 1496 2008
Fragments arranged by size:
2008 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1643 2164 2842 3253 3400 3674 1643
Size: 521 678 411 147 274 2423
Fragments arranged by size:
2423 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 137 326 535 954 137
Size: 189 209 419 3637
Fragments arranged by size:
3637 419 209 189
BseYI C'CCAG_C
Cuts at: 1879 2637 3302 3710 1879
Size: 758 665 408 2623
Fragments arranged by size:
2623 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1996 2042 3272 104
Size: 1892 46 1230 1286
Fragments arranged by size:
1892 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1468 2246 2436 3296 1468
Size: 778 190 860 2626
Fragments arranged by size:
2626 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 1468 2079 2172 2246 2436 2498 3296 1468
Size: 611 93 74 190 62 798 2626
Fragments arranged by size:
2626 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 1178 2886 1178
Size: 1708 2746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1504 1718 2020 2401 1504
Size: 214 302 381 3557
Fragments arranged by size:
3557 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2746 2800 4284 4449 50
Size: 2696 54 1484 165 55
Fragments arranged by size:
2696 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1397 2366 1397
Size: 969 3485
BsrFI r'CCGG_y
Cuts at: 2452 2633 2452
Size: 181 4273
BssSI C'ACGA_G
Cuts at: 2725 3433 2725
Size: 708 3746
BstAPI GCAn_nnn'nTGC
Cuts at: 1831 1831
Size: 4454
BstBI TT'CG_AA
Cuts at: 2815 2815
Size: 4454
BstEII G'GTnAC_C
Cuts at: 410 410
Size: 4454
BstYI r'GATC_y
Cuts at: 1 540 588 927 1162 1186 1479 1946 1969
Size: 539 48 339 235 24 293 467 23
Cuts at: 1969 2304 2550 2856 2868 2954 4006 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 539 467 449 339 335 306 293
246 235 86 48 24 23 12
BstZ17I GTA'TAC
Cuts at: 837 837
Size: 4454
BtgI C'CryG_G
Cuts at: 107 107
Size: 4454
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1235 2404 2585 2748 1235
Size: 1169 181 163 2941
Fragments arranged by size:
2941 1169 181 163
BtsI GCAGTG_nn'
Cuts at: 1697 1697
Size: 4454
DraI TTT'AAA
Cuts at: 1453 1453
Size: 4454
DrdI GACnn_nn'nnGTC
Cuts at: 1174 2160 3504 1174
Size: 986 1344 2124
Fragments arranged by size:
2124 1344 986
EaeI y'GGCC_r
Cuts at: 2039 2213 2604 2631 3976 2039
Size: 174 391 27 1345 2517
Fragments arranged by size:
2517 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2039 2039
Size: 4454
EarI CTCTTCn'nnn_
Cuts at: 770 2477 2687 770
Size: 1707 210 2537
Fragments arranged by size:
2537 1707 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2662 3404 3550 4264 2662
Size: 742 146 714 2852
Fragments arranged by size:
2852 742 714 146
EcoICRI GAG'CTC
Cuts at: 1466 1466
Size: 4454
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 245 944 1188 1303 2279 2711 3064 3726
Size: 105 699 244 115 976 432 353 662
Cuts at: 3726 140
Size: 868
Fragments arranged by size:
976 868 699 662 432 353 244 115
105
EcoO109I rG'GnC_Cy
Cuts at: 1593 1593
Size: 4454
EcoRI G'AATT_C
Cuts at: 1458 1458
Size: 4454
EcoRV GAT'ATC
Cuts at: 756 756
Size: 4454
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4454
FspI TGC'GCA
Cuts at: 2235 2235
Size: 4454
HaeII r_GCGC'y
Cuts at: 1888 2136 3366 4137 1888
Size: 248 1230 771 2205
Fragments arranged by size:
2205 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 251 283 557 589 1009 1041 2296 2326 2328
Size: 32 274 32 420 32 1255 30 2
Cuts at: 2328 2358 251
Size: 30 2347
Fragments arranged by size:
2347 1255 420 274 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1338 1493 1338
Size: 155 4299
KasI G'GCGC_C
Cuts at: 1884 2132 1884
Size: 248 4206
KpnI G_GTAC'C
Cuts at: 1474 1474
Size: 4454
MluI A'CGCG_T
Cuts at: 3887 3887
Size: 4454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1135 3213 3397 1135
Size: 2078 184 2192
Fragments arranged by size:
2192 2078 184
MscI TGG'CCA
Cuts at: 2215 2215
Size: 4454
MslI CAynn'nnrTG
Cuts at: 2443 2570 2443
Size: 127 4327
MspA1I CmG'CkG
Cuts at: 821 1565 1879 2239 3023 3268 821
Size: 744 314 360 784 245 2007
Fragments arranged by size:
2007 784 744 360 314 245
NaeI GCC'GGC
Cuts at: 2635 2635
Size: 4454
NarI GG'CG_CC
Cuts at: 1885 2133 1885
Size: 248 4206
NcoI C'CATG_G
Cuts at: 107 107
Size: 4454
NgoMIV G'CCGG_C
Cuts at: 2633 2633
Size: 4454
NspI r_CATG'y
Cuts at: 1507 2007 3610 1507
Size: 500 1603 2351
Fragments arranged by size:
2351 1603 500
PciI A'CATG_T
Cuts at: 3606 3606
Size: 4454
PmeI GTTT'AAAC
Cuts at: 1453 1453
Size: 4454
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2900 2932 2900
Size: 32 4422
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1092 1124 1092
Size: 32 4422
PstI C_TGCA'G
Cuts at: 693 1501 2186 693
Size: 808 685 2961
Fragments arranged by size:
2961 808 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4454
PvuII CAG'CTG
Cuts at: 821 1879 2239 821
Size: 1058 360 3036
Fragments arranged by size:
3036 1058 360
RsrII CG'GwC_CG
Cuts at: 2649 2649
Size: 4454
SacI G_AGCT'C
Cuts at: 1468 1468
Size: 4454
SalI G'TCGA_C
Cuts at: 1491 1491
Size: 4454
SapI GCTCTTCn'nnn_
Cuts at: 770 2477 2687 770
Size: 1707 210 2537
Fragments arranged by size:
2537 1707 210
SbfI CC_TGCA'GG
Cuts at: 1501 1501
Size: 4454
ScaI AGT'ACT
Cuts at: 3971 3971
Size: 4454
SexAI A'CCwGG_T
Cuts at: 1076 1076
Size: 4454
SfcI C'TryA_G
Cuts at: 33 203 548 689 1497 2182 3150 3341 33
Size: 170 345 141 808 685 968 191 1146
Fragments arranged by size:
1146 968 808 685 345 191 170 141
SfoI GGC'GCC
Cuts at: 1886 2134 1886
Size: 248 4206
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4454
SmaI CCC'GGG
Cuts at: 1476 1476
Size: 4454
SmlI C'TyrA_G
Cuts at: 1609 2524 3238 3500 1609
Size: 915 714 262 2563
Fragments arranged by size:
2563 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4454
SphI G_CATG'C
Cuts at: 1507 2007 1507
Size: 500 3954
SspI AAT'ATT
Cuts at: 620 620
Size: 4454
StuI AGG'CCT
Cuts at: 582 1062 582
Size: 480 3974
StyI C'CwwG_G
Cuts at: 107 1385 1588 1942 107
Size: 1278 203 354 2619
Fragments arranged by size:
2619 1278 354 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2037 2170 2629 2838 2037
Size: 133 459 209 3653
Fragments arranged by size:
3653 459 209 133
TatI w'GTAC_w
Cuts at: 3969 3969
Size: 4454
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 465 497 3971 4003 465
Size: 32 3474 32 916
Fragments arranged by size:
3474 916 32 32
Tth111I GACn'n_nGTC
Cuts at: 2251 2251
Size: 4454
XbaI T'CTAG_A
Cuts at: 1485 1485
Size: 4454
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1949 1949
Size: 4454
XmaI C'CCGG_G
Cuts at: 1474 1474
Size: 4454
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BspEI BsrGI BssHII BstXI Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI
SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI