Restriction map for
pF1KB5503
(Circular) (Six-base) MAPSORT of: pF1KB5503.seq Check: 1435 from: 1 to: 4778
pF1KB5503 4778 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 836 1816 836
Size: 980 3798
Acc65I G'GTAC_C
Cuts at: 1794 1794
Size: 4778
AclI AA'CG_TT
Cuts at: 4618 4618
Size: 4778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 783 2603 3035 3782 4050 262
Size: 521 1820 432 747 268 990
Fragments arranged by size:
1820 990 747 521 432 268
AfeI AGC'GCT
Cuts at: 750 750
Size: 4778
AflIII A'CryG_T
Cuts at: 2130 3234 4211 2130
Size: 1104 977 2697
Fragments arranged by size:
2697 1104 977
AgeI A'CCGG_T
Cuts at: 3166 3166
Size: 4778
AhdI GACnn_n'nnGTC
Cuts at: 822 822
Size: 4778
AleI CACnn'nnGTG
Cuts at: 307 364 307
Size: 57 4721
AlwNI CAG_nnn'CTG
Cuts at: 1452 2083 3650 1452
Size: 631 1567 2580
Fragments arranged by size:
2580 1567 631
ApaI G_GGCC'C
Cuts at: 599 599
Size: 4778
ApaLI G'TGCA_C
Cuts at: 3548 3548
Size: 4778
ApoI r'AATT_y
Cuts at: 1782 4131 4387 1782
Size: 2349 256 2173
Fragments arranged by size:
2349 2173 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4778
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4778
AvaI C'yCGr_G
Cuts at: 149 1798 149
Size: 1649 3129
BamHI G'GATC_C
Cuts at: 1042 1803 1042
Size: 761 4017
BanI G'GyrC_C
Cuts at: 294 895 1553 1794 2208 2456 2491 294
Size: 601 658 241 414 248 35 2581
Fragments arranged by size:
2581 658 601 414 248 241 35
BanII G_rGCy'C
Cuts at: 529 599 1395 1792 2822 529
Size: 70 796 397 1030 2485
Fragments arranged by size:
2485 1030 796 397 70
BbeI G_GCGC'C
Cuts at: 2212 2460 2212
Size: 248 4530
BbsI GAAGACnn'nnnn_
Cuts at: 223 1771 223
Size: 1548 3230
BciVI GTATCCnnnnn_n'
Cuts at: 2676 3437 4778 2676
Size: 761 1341 2676
Fragments arranged by size:
2676 1341 761
BclI T'GATC_A
Cuts at: 2298 2298
Size: 4778
BglI GCCn_nnn'nGGC
Cuts at: 112 121 112
Size: 9 4769
BglII A'GATC_T
Cuts at: 1724 2293 4330 1724
Size: 569 2037 2172
Fragments arranged by size:
2172 2037 569
BlpI GC'TnA_GC
Cuts at: 1307 1890 1307
Size: 583 4195
Bme1580I G_kGCm'C
Cuts at: 332 599 1556 1703 2403 2496 3552 332
Size: 267 957 147 700 93 1056 1558
Fragments arranged by size:
1558 1056 957 700 267 147 93
BmgBI CAC'GTC
Cuts at: 1488 1488
Size: 4778
BmrI ACTGGGnnnn_n'
Cuts at: 849 1018 1990 2071 2406 4550 849
Size: 169 972 81 335 2144 1077
Fragments arranged by size:
2144 1077 972 335 169 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 782 3145 782
Size: 2363 2415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 963 1124 1753 1770 963
Size: 161 629 17 3971
Fragments arranged by size:
3971 629 161 17
Bpu10I CC'TnA_GC
Cuts at: 1094 1094
Size: 4778
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1954 2833 3325 3623 754
Size: 1200 879 492 298 1909
Fragments arranged by size:
1909 1200 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 220 1729 4482 220
Size: 1509 2753 516
Fragments arranged by size:
2753 1509 516
BsaAI yAC'GTr
Cuts at: 543 1089 2131 543
Size: 546 1042 3190
Fragments arranged by size:
3190 1042 546
BsaBI GATnn'nnATC
Cuts at: 6 2292 4329 6
Size: 2286 2037 455
Fragments arranged by size:
2286 2037 455
BsaHI Gr'CG_yC
Cuts at: 1546 2209 2457 3159 4655 1546
Size: 663 248 702 1496 1669
Fragments arranged by size:
1669 1496 702 663 248
BsaWI w'CCGG_w
Cuts at: 1967 2488 3166 3440 3587 3998 1967
Size: 521 678 274 147 411 2747
Fragments arranged by size:
2747 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 448 478 448
Size: 30 4748
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 466 469 549 1618 466
Size: 3 80 1069 3626
Fragments arranged by size:
3626 1069 80 3
BseYI C'CCAG_C
Cuts at: 195 1038 1172 1313 2203 2961 3538 4034 195
Size: 843 134 141 890 758 577 496 939
Fragments arranged by size:
939 890 843 758 577 496 141 134
BsiEI CG_ry'CG
Cuts at: 104 116 2320 2366 3574 104
Size: 12 2204 46 1208 1308
Fragments arranged by size:
2204 1308 1208 46 12
BsiHKAI G_wGCw'C
Cuts at: 1073 1792 2570 2760 3552 1073
Size: 719 778 190 792 2299
Fragments arranged by size:
2299 792 778 719 190
BsmI GAATG_Cn'
Cuts at: 276 891 276
Size: 615 4163
Bsp1286I G_dGCh'C
Cuts at: 332 529 599 1073 1395 1556 1703 1792 2403
Size: 197 70 474 322 161 147 89 611
Cuts at: 2403 2496 2570 2760 2822 3552 332
Size: 93 74 190 62 730 1558
Fragments arranged by size:
1558 730 611 474 322 197 190 161
147 93 89 74 70 62
BspEI T'CCGG_A
Cuts at: 3998 3998
Size: 4778
BspHI T'CATG_A
Cuts at: 3954 3954
Size: 4778
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1828 2042 2344 2725 1828
Size: 214 302 381 3881
Fragments arranged by size:
3881 381 302 214
BsrBI CCG'CTC
Cuts at: 50 207 3070 3124 4608 4773 50
Size: 157 2863 54 1484 165 55
Fragments arranged by size:
2863 1484 165 157 55 54
BsrDI GCAATG_nn'
Cuts at: 947 2690 947
Size: 1743 3035
BsrFI r'CCGG_y
Cuts at: 1379 2776 2957 3166 1379
Size: 1397 181 209 2991
Fragments arranged by size:
2991 1397 209 181
BsrGI T'GTAC_A
Cuts at: 1115 1502 1115
Size: 387 4391
BssHII G'CGCG_C
Cuts at: 159 159
Size: 4778
BssSI C'ACGA_G
Cuts at: 3049 3407 3049
Size: 358 4420
BstAPI GCAn_nnn'nTGC
Cuts at: 2155 2155
Size: 4778
BstBI TT'CG_AA
Cuts at: 3139 3139
Size: 4778
BstXI CCAn_nnnn'nTGG
Cuts at: 195 262 195
Size: 67 4711
BstYI r'GATC_y
Cuts at: 1 1042 1724 1803 2270 2293 2628 2874 3886
Size: 1041 682 79 467 23 335 246 1012
Cuts at: 3886 3972 3984 4330 1
Size: 86 12 346 449
Fragments arranged by size:
1041 1012 682 467 449 346 335 246
86 79 23 12
Bsu36I CC'TnA_GG
Cuts at: 509 509
Size: 4778
BtgI C'CryG_G
Cuts at: 107 221 107
Size: 114 4664
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2728 2909 3072 2728
Size: 181 163 4434
Fragments arranged by size:
4434 181 163
BtsI GCAGTG_nn'
Cuts at: 480 617 2021 480
Size: 137 1404 3237
Fragments arranged by size:
3237 1404 137
DraI TTT'AAA
Cuts at: 1777 1777
Size: 4778
DrdI GACnn_nn'nnGTC
Cuts at: 1743 2484 3342 1743
Size: 741 858 3179
Fragments arranged by size:
3179 858 741
EaeI y'GGCC_r
Cuts at: 113 185 654 1145 1412 2363 2537 2928 2955
Size: 72 469 491 267 951 174 391 27
Cuts at: 2955 4300 113
Size: 1345 591
Fragments arranged by size:
1345 951 591 491 469 391 267 174
72 27
EagI C'GGCC_G
Cuts at: 113 2363 113
Size: 2250 2528
EarI CTCTTCn'nnn_
Cuts at: 175 751 1532 1687 2801 3011 175
Size: 576 781 155 1114 210 1942
Fragments arranged by size:
1942 1114 781 576 210 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 274 2986 3296 3442 4588 274
Size: 2712 310 146 1146 464
Fragments arranged by size:
2712 1146 464 310 146
EcoICRI GAG'CTC
Cuts at: 1790 1790
Size: 4778
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 783 963 1124 1753 1770 2603 3035 3782
Size: 521 180 161 629 17 833 432 747
Cuts at: 3782 4050 262
Size: 268 990
Fragments arranged by size:
990 833 747 629 521 432 268 180
161 17
EcoNI CCTnn'n_nnAGG
Cuts at: 432 733 432
Size: 301 4477
EcoO109I rG'GnC_Cy
Cuts at: 459 1342 1917 459
Size: 883 575 3320
Fragments arranged by size:
3320 883 575
EcoRI G'AATT_C
Cuts at: 1782 1782
Size: 4778
EcoRV GAT'ATC
Cuts at: 548 548
Size: 4778
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 372 795 2797 372
Size: 423 2002 2353
Fragments arranged by size:
2353 2002 423
FspI TGC'GCA
Cuts at: 2559 2559
Size: 4778
HaeII r_GCGC'y
Cuts at: 752 867 1139 2212 2460 3482 4461 752
Size: 115 272 1073 248 1022 979 1069
Fragments arranged by size:
1073 1069 1022 979 272 248 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 448 480 808 840 1477 1509 1531 1563 2620
Size: 32 328 32 637 32 22 32 1057
Cuts at: 2620 2650 2652 2682 448
Size: 30 2 30 2544
Fragments arranged by size:
2544 1057 637 328 32 32 32 32
30 30 22 2
HincII GTy'rAC
Cuts at: 1200 1817 1200
Size: 617 4161
KasI G'GCGC_C
Cuts at: 2208 2456 2208
Size: 248 4530
KpnI G_GTAC'C
Cuts at: 1798 1798
Size: 4778
MluI A'CGCG_T
Cuts at: 4211 4211
Size: 4778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3449 3633 3449
Size: 184 4594
MscI TGG'CCA
Cuts at: 187 1147 1414 2539 187
Size: 960 267 1125 2426
Fragments arranged by size:
2426 1125 960 267
MslI CAynn'nnrTG
Cuts at: 307 364 2767 2894 307
Size: 57 2403 127 2191
Fragments arranged by size:
2403 2191 127 57
MspA1I CmG'CkG
Cuts at: 901 1113 1172 1317 1889 2203 2563 3576 3821
Size: 212 59 145 572 314 360 1013 245
Cuts at: 3821 901
Size: 1858
Fragments arranged by size:
1858 1013 572 360 314 245 212 145
59
NaeI GCC'GGC
Cuts at: 2959 2959
Size: 4778
NarI GG'CG_CC
Cuts at: 2209 2457 2209
Size: 248 4530
NcoI C'CATG_G
Cuts at: 107 107
Size: 4778
NgoMIV G'CCGG_C
Cuts at: 2957 2957
Size: 4778
NotI GC'GGCC_GC
Cuts at: 113 113
Size: 4778
NspI r_CATG'y
Cuts at: 999 1831 2331 3238 999
Size: 832 500 907 2539
Fragments arranged by size:
2539 907 832 500
PciI A'CATG_T
Cuts at: 3234 3234
Size: 4778
PfoI T'CCnGG_A
Cuts at: 1730 1747 1730
Size: 17 4761
PmeI GTTT'AAAC
Cuts at: 1777 1777
Size: 4778
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 3917
Size: 32 4746
PspOMI G'GGCC_C
Cuts at: 595 595
Size: 4778
PstI C_TGCA'G
Cuts at: 1825 2510 1825
Size: 685 4093
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4778
PvuII CAG'CTG
Cuts at: 1317 2203 2563 1317
Size: 886 360 3532
Fragments arranged by size:
3532 886 360
RsrII CG'GwC_CG
Cuts at: 2973 2973
Size: 4778
SacI G_AGCT'C
Cuts at: 1792 1792
Size: 4778
SalI G'TCGA_C
Cuts at: 1815 1815
Size: 4778
SapI GCTCTTCn'nnn_
Cuts at: 2801 3011 2801
Size: 210 4568
SbfI CC_TGCA'GG
Cuts at: 1825 1825
Size: 4778
ScaI AGT'ACT
Cuts at: 4295 4295
Size: 4778
SfcI C'TryA_G
Cuts at: 33 1511 1821 2506 3499 3690 33
Size: 1478 310 685 993 191 1121
Fragments arranged by size:
1478 1121 993 685 310 191
SfoI GGC'GCC
Cuts at: 2210 2458 2210
Size: 248 4530
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4778
SmaI CCC'GGG
Cuts at: 151 1800 151
Size: 1649 3129
SmlI C'TyrA_G
Cuts at: 769 1933 2848 3340 3602 769
Size: 1164 915 492 262 1945
Fragments arranged by size:
1945 1164 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4778
SphI G_CATG'C
Cuts at: 1831 2331 1831
Size: 500 4278
StuI AGG'CCT
Cuts at: 427 640 427
Size: 213 4565
StyI C'CwwG_G
Cuts at: 107 1912 2266 107
Size: 1805 354 2619
Fragments arranged by size:
2619 1805 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1385 2361 2494 2953 3162 1385
Size: 976 133 459 209 3001
Fragments arranged by size:
3001 976 459 209 133
TatI w'GTAC_w
Cuts at: 1115 1502 4293 1115
Size: 387 2791 1600
Fragments arranged by size:
2791 1600 387
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4295 4327 4295
Size: 32 4746
Tth111I GACn'n_nGTC
Cuts at: 2575 2575
Size: 4778
XbaI T'CTAG_A
Cuts at: 1809 1809
Size: 4778
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 740 987 2273 740
Size: 247 1286 3245
Fragments arranged by size:
3245 1286 247
XmaI C'CCGG_G
Cuts at: 149 1798 149
Size: 1649 3129
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PciI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BsgI BsiWI BsmBI BstEII
BstZ17I ClaI CspCI DraIII FseI FspAI HindIII HpaI
MfeI NdeI NheI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI