Restriction map for
pF1KB5427
(Circular) (Six-base) MAPSORT of: pF1KB5427.seq Check: 965 from: 1 to: 4247
pF1KB5427 4247 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 666 666
Size: 4247
AccI GT'mk_AC
Cuts at: 1285 1285
Size: 4247
Acc65I G'GTAC_C
Cuts at: 1263 1263
Size: 4247
AclI AA'CG_TT
Cuts at: 4087 4087
Size: 4247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 920 2072 2504 3251 3519 148
Size: 772 1152 432 747 268 876
Fragments arranged by size:
1152 876 772 747 432 268
AflIII A'CryG_T
Cuts at: 1599 2703 3680 1599
Size: 1104 977 2166
Fragments arranged by size:
2166 1104 977
AgeI A'CCGG_T
Cuts at: 2635 2635
Size: 4247
AlwNI CAG_nnn'CTG
Cuts at: 141 1083 1233 1552 3119 141
Size: 942 150 319 1567 1269
Fragments arranged by size:
1567 1269 942 319 150
ApaLI G'TGCA_C
Cuts at: 3017 3017
Size: 4247
ApoI r'AATT_y
Cuts at: 1251 3600 3856 1251
Size: 2349 256 1642
Fragments arranged by size:
2349 1642 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4247
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
AvaI C'yCGr_G
Cuts at: 604 683 1267 604
Size: 79 584 3584
Fragments arranged by size:
3584 584 79
BamHI G'GATC_C
Cuts at: 878 1272 878
Size: 394 3853
BanI G'GyrC_C
Cuts at: 982 1005 1263 1677 1925 1960 982
Size: 23 258 414 248 35 3269
Fragments arranged by size:
3269 414 258 248 35 23
BanII G_rGCy'C
Cuts at: 460 1201 1261 2291 460
Size: 741 60 1030 2416
Fragments arranged by size:
2416 1030 741 60
BbeI G_GCGC'C
Cuts at: 1681 1929 1681
Size: 248 3999
BbsI GAAGACnn'nnnn_
Cuts at: 147 525 896 908 1100 1131 147
Size: 378 371 12 192 31 3263
Fragments arranged by size:
3263 378 371 192 31 12
BciVI GTATCCnnnnn_n'
Cuts at: 2145 2906 4247 2145
Size: 761 1341 2145
Fragments arranged by size:
2145 1341 761
BclI T'GATC_A
Cuts at: 1094 1767 1094
Size: 673 3574
BglI GCCn_nnn'nGGC
Cuts at: 1014 1014
Size: 4247
BglII A'GATC_T
Cuts at: 1762 3799 1762
Size: 2037 2210
BlpI GC'TnA_GC
Cuts at: 1359 1359
Size: 4247
Bme1580I G_kGCm'C
Cuts at: 319 640 985 1010 1872 1965 3021 319
Size: 321 345 25 862 93 1056 1545
Fragments arranged by size:
1545 1056 862 345 321 93 25
BmrI ACTGGGnnnn_n'
Cuts at: 1459 1540 1875 4019 1459
Size: 81 335 2144 1687
Fragments arranged by size:
2144 1687 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 4247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 444 322
Size: 122 4125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 340 691 780 1423 2302 2794 3092 264
Size: 76 351 89 643 879 492 298 1419
Fragments arranged by size:
1419 879 643 492 351 298 89 76
BsaI GGTCTCn'nnnn_
Cuts at: 3951 3951
Size: 4247
BsaAI yAC'GTr
Cuts at: 1600 1600
Size: 4247
BsaBI GATnn'nnATC
Cuts at: 6 1761 3798 6
Size: 1755 2037 455
Fragments arranged by size:
2037 1755 455
BsaHI Gr'CG_yC
Cuts at: 997 1678 1926 2628 4124 997
Size: 681 248 702 1496 1120
Fragments arranged by size:
1496 1120 702 681 248
BsaWI w'CCGG_w
Cuts at: 245 1436 1957 2635 2909 3056 3467 245
Size: 1191 521 678 274 147 411 1025
Fragments arranged by size:
1191 1025 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 295 325 295
Size: 30 4217
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 208 768 979 205
Size: 3 560 211 3473
Fragments arranged by size:
3473 560 211 3
BseYI C'CCAG_C
Cuts at: 828 1672 2430 3007 3503 828
Size: 844 758 577 496 1572
Fragments arranged by size:
1572 844 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 913 913
Size: 4247
BsiEI CG_ry'CG
Cuts at: 104 1789 1835 3043 104
Size: 1685 46 1208 1308
Fragments arranged by size:
1685 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 919 1064 1261 2039 2229 3021 919
Size: 145 197 778 190 792 2145
Fragments arranged by size:
2145 792 778 197 190 145
BsmI GAATG_Cn'
Cuts at: 328 328
Size: 4247
BsmBI CGTCTCn'nnnn_
Cuts at: 988 988
Size: 4247
Bsp1286I G_dGCh'C
Cuts at: 319 460 640 919 985 1010 1064 1201 1261
Size: 141 180 279 66 25 54 137 60
Cuts at: 1261 1872 1965 2039 2229 2291 3021 319
Size: 611 93 74 190 62 730 1545
Fragments arranged by size:
1545 730 611 279 190 180 141 137
93 74 66 62 60 54 25
BspEI T'CCGG_A
Cuts at: 245 3467 245
Size: 3222 1025
BspHI T'CATG_A
Cuts at: 3423 3423
Size: 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 666 1297 1511 1813 2194 666
Size: 631 214 302 381 2719
Fragments arranged by size:
2719 631 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1103 2539 2593 4077 4242 50
Size: 1053 1436 54 1484 165 55
Fragments arranged by size:
1484 1436 1053 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2159 2159
Size: 4247
BsrFI r'CCGG_y
Cuts at: 2245 2426 2635 2245
Size: 181 209 3857
Fragments arranged by size:
3857 209 181
BsrGI T'GTAC_A
Cuts at: 790 790
Size: 4247
BssHII G'CGCG_C
Cuts at: 345 345
Size: 4247
BssSI C'ACGA_G
Cuts at: 1030 2518 2876 1030
Size: 1488 358 2401
Fragments arranged by size:
2401 1488 358
BstAPI GCAn_nnn'nTGC
Cuts at: 323 1624 323
Size: 1301 2946
BstBI TT'CG_AA
Cuts at: 2608 2608
Size: 4247
BstXI CCAn_nnnn'nTGG
Cuts at: 468 468
Size: 4247
BstYI r'GATC_y
Cuts at: 1 878 1272 1739 1762 2097 2343 3355 3441
Size: 877 394 467 23 335 246 1012 86
Cuts at: 3441 3453 3799 1
Size: 12 346 449
Fragments arranged by size:
1012 877 467 449 394 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 177 177
Size: 4247
BtgI C'CryG_G
Cuts at: 107 1001 107
Size: 894 3353
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2197 2378 2541 2197
Size: 181 163 3903
Fragments arranged by size:
3903 181 163
BtsI GCAGTG_nn'
Cuts at: 823 1490 823
Size: 667 3580
DraI TTT'AAA
Cuts at: 1246 1246
Size: 4247
DrdI GACnn_nn'nnGTC
Cuts at: 1953 2811 1953
Size: 858 3389
EaeI y'GGCC_r
Cuts at: 470 1121 1832 2006 2397 2424 3769 470
Size: 651 711 174 391 27 1345 948
Fragments arranged by size:
1345 948 711 651 391 174 27
EagI C'GGCC_G
Cuts at: 1832 1832
Size: 4247
EarI CTCTTCn'nnn_
Cuts at: 211 247 2270 2480 211
Size: 36 2023 210 1978
Fragments arranged by size:
2023 1978 210 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2455 2765 2911 4057 2455
Size: 310 146 1146 2645
Fragments arranged by size:
2645 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1259 1259
Size: 4247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 322 444 920 2072 2504 3251 3519 148
Size: 174 122 476 1152 432 747 268 876
Fragments arranged by size:
1152 876 747 476 432 268 174 122
EcoO109I rG'GnC_Cy
Cuts at: 465 1386 465
Size: 921 3326
EcoRI G'AATT_C
Cuts at: 1251 1251
Size: 4247
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 258 972 2266 258
Size: 714 1294 2239
Fragments arranged by size:
2239 1294 714
FspI TGC'GCA
Cuts at: 2028 2028
Size: 4247
HaeII r_GCGC'y
Cuts at: 1681 1929 2951 3930 1681
Size: 248 1022 979 1998
Fragments arranged by size:
1998 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 295 327 901 933 1183 1215 2089 2119 2121
Size: 32 574 32 250 32 874 30 2
Cuts at: 2121 2151 295
Size: 30 2391
Fragments arranged by size:
2391 874 574 250 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1286 1286
Size: 4247
KasI G'GCGC_C
Cuts at: 1677 1925 1677
Size: 248 3999
KpnI G_GTAC'C
Cuts at: 1267 1267
Size: 4247
MluI A'CGCG_T
Cuts at: 3680 3680
Size: 4247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 844 2918 3102 844
Size: 2074 184 1989
Fragments arranged by size:
2074 1989 184
MscI TGG'CCA
Cuts at: 472 1123 2008 472
Size: 651 885 2711
Fragments arranged by size:
2711 885 651
MslI CAynn'nnrTG
Cuts at: 526 676 748 2236 2363 526
Size: 150 72 1488 127 2410
Fragments arranged by size:
2410 1488 150 127 72
MspA1I CmG'CkG
Cuts at: 288 778 1358 1672 2032 3045 3290 288
Size: 490 580 314 360 1013 245 1245
Fragments arranged by size:
1245 1013 580 490 360 314 245
NaeI GCC'GGC
Cuts at: 2428 2428
Size: 4247
NarI GG'CG_CC
Cuts at: 1678 1926 1678
Size: 248 3999
NcoI C'CATG_G
Cuts at: 107 1001 107
Size: 894 3353
NgoMIV G'CCGG_C
Cuts at: 2426 2426
Size: 4247
NspI r_CATG'y
Cuts at: 675 1300 1800 2707 675
Size: 625 500 907 2215
Fragments arranged by size:
2215 907 625 500
PciI A'CATG_T
Cuts at: 2703 2703
Size: 4247
PflMI CCAn_nnn'nTGG
Cuts at: 1083 1083
Size: 4247
PfoI T'CCnGG_A
Cuts at: 593 593
Size: 4247
PmeI GTTT'AAAC
Cuts at: 1246 1246
Size: 4247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3386 3418 3386
Size: 32 4215
PpuMI rG'GwC_Cy
Cuts at: 465 465
Size: 4247
PstI C_TGCA'G
Cuts at: 262 1294 1979 262
Size: 1032 685 2530
Fragments arranged by size:
2530 1032 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4247
PvuII CAG'CTG
Cuts at: 288 778 1672 2032 288
Size: 490 894 360 2503
Fragments arranged by size:
2503 894 490 360
RsrII CG'GwC_CG
Cuts at: 2442 2442
Size: 4247
SacI G_AGCT'C
Cuts at: 1261 1261
Size: 4247
SalI G'TCGA_C
Cuts at: 1284 1284
Size: 4247
SapI GCTCTTCn'nnn_
Cuts at: 211 247 2270 2480 211
Size: 36 2023 210 1978
Fragments arranged by size:
2023 1978 210 36
SbfI CC_TGCA'GG
Cuts at: 1294 1294
Size: 4247
ScaI AGT'ACT
Cuts at: 970 3764 970
Size: 2794 1453
SexAI A'CCwGG_T
Cuts at: 149 149
Size: 4247
SfcI C'TryA_G
Cuts at: 33 258 972 1290 1975 2968 3159 33
Size: 225 714 318 685 993 191 1121
Fragments arranged by size:
1121 993 714 685 318 225 191
SfoI GGC'GCC
Cuts at: 1679 1927 1679
Size: 248 3999
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
SmaI CCC'GGG
Cuts at: 685 1269 685
Size: 584 3663
SmlI C'TyrA_G
Cuts at: 279 355 706 759 1402 2317 2809 3071 279
Size: 76 351 53 643 915 492 262 1455
Fragments arranged by size:
1455 915 643 492 351 262 76 53
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4247
SphI G_CATG'C
Cuts at: 675 1300 1800 675
Size: 625 500 3122
Fragments arranged by size:
3122 625 500
SrfI GCCC'GGGC
Cuts at: 685 685
Size: 4247
StyI C'CwwG_G
Cuts at: 107 461 689 731 1001 1381 1735 107
Size: 354 228 42 270 380 354 2619
Fragments arranged by size:
2619 380 354 354 270 228 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1830 1963 2422 2631 1830
Size: 133 459 209 3446
Fragments arranged by size:
3446 459 209 133
TatI w'GTAC_w
Cuts at: 790 968 3762 790
Size: 178 2794 1275
Fragments arranged by size:
2794 1275 178
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1161 1193 3764 3796 1161
Size: 32 2571 32 1612
Fragments arranged by size:
2571 1612 32 32
Tth111I GACn'n_nGTC
Cuts at: 2044 2044
Size: 4247
XbaI T'CTAG_A
Cuts at: 1278 1278
Size: 4247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 480 503 1742 480
Size: 23 1239 2985
Fragments arranged by size:
2985 1239 23
XmaI C'CCGG_G
Cuts at: 683 1267 683
Size: 584 3663
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BstEII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SspI StuI SwaI XhoI XmnI ZraI