Restriction map for
pF1KB5419
(Circular) (Six-base) MAPSORT of: pF1KB5419.seq Check: 8919 from: 1 to: 4067
pF1KB5419 4067 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 641 1105 641
Size: 464 3603
Acc65I G'GTAC_C
Cuts at: 882 1083 882
Size: 201 3866
AclI AA'CG_TT
Cuts at: 3907 3907
Size: 4067
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 630 1892 2324 3071 3339 630
Size: 1262 432 747 268 1358
Fragments arranged by size:
1358 1262 747 432 268
AflIII A'CryG_T
Cuts at: 1419 2523 3500 1419
Size: 1104 977 1986
Fragments arranged by size:
1986 1104 977
AgeI A'CCGG_T
Cuts at: 2455 2455
Size: 4067
AlwNI CAG_nnn'CTG
Cuts at: 1372 2939 1372
Size: 1567 2500
ApaLI G'TGCA_C
Cuts at: 2837 2837
Size: 4067
ApoI r'AATT_y
Cuts at: 164 1053 1071 3420 3676 164
Size: 889 18 2349 256 555
Fragments arranged by size:
2349 889 555 256 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 4067
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
AvaI C'yCGr_G
Cuts at: 1087 1087
Size: 4067
BamHI G'GATC_C
Cuts at: 1092 1092
Size: 4067
BanI G'GyrC_C
Cuts at: 882 1083 1497 1745 1780 882
Size: 201 414 248 35 3169
Fragments arranged by size:
3169 414 248 201 35
BanII G_rGCy'C
Cuts at: 1081 2111 1081
Size: 1030 3037
BbeI G_GCGC'C
Cuts at: 1501 1749 1501
Size: 248 3819
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4033
BciVI GTATCCnnnnn_n'
Cuts at: 1965 2726 4067 1965
Size: 761 1341 1965
Fragments arranged by size:
1965 1341 761
BclI T'GATC_A
Cuts at: 1587 1587
Size: 4067
BglII A'GATC_T
Cuts at: 1582 3619 1582
Size: 2037 2030
BlpI GC'TnA_GC
Cuts at: 1179 1179
Size: 4067
Bme1580I G_kGCm'C
Cuts at: 1692 1785 2841 1692
Size: 93 1056 2918
Fragments arranged by size:
2918 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1279 1360 1695 3839 1279
Size: 81 335 2144 1507
Fragments arranged by size:
2144 1507 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1243 2122 2614 2912 1243
Size: 879 492 298 2398
Fragments arranged by size:
2398 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3771 3771
Size: 4067
BsaAI yAC'GTr
Cuts at: 1420 1420
Size: 4067
BsaBI GATnn'nnATC
Cuts at: 6 667 1581 3618 6
Size: 661 914 2037 455
Fragments arranged by size:
2037 914 661 455
BsaHI Gr'CG_yC
Cuts at: 1498 1746 2448 3944 1498
Size: 248 702 1496 1621
Fragments arranged by size:
1621 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1256 1777 2455 2729 2876 3287 1256
Size: 521 678 274 147 411 2036
Fragments arranged by size:
2036 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 229 1492 2250 2827 3323 229
Size: 1263 758 577 496 973
Fragments arranged by size:
1263 973 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1609 1655 2863 104
Size: 1505 46 1208 1308
Fragments arranged by size:
1505 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1081 1859 2049 2841 1081
Size: 778 190 792 2307
Fragments arranged by size:
2307 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1081 1692 1785 1859 2049 2111 2841 1081
Size: 611 93 74 190 62 730 2307
Fragments arranged by size:
2307 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3287 3287
Size: 4067
BspHI T'CATG_A
Cuts at: 620 3243 620
Size: 2623 1444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1117 1331 1633 2014 1117
Size: 214 302 381 3170
Fragments arranged by size:
3170 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2359 2413 3897 4062 50
Size: 2309 54 1484 165 55
Fragments arranged by size:
2309 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 281 1979 281
Size: 1698 2369
BsrFI r'CCGG_y
Cuts at: 2065 2246 2455 2065
Size: 181 209 3677
Fragments arranged by size:
3677 209 181
BsrGI T'GTAC_A
Cuts at: 899 899
Size: 4067
BssSI C'ACGA_G
Cuts at: 2338 2696 2338
Size: 358 3709
BstAPI GCAn_nnn'nTGC
Cuts at: 1444 1444
Size: 4067
BstBI TT'CG_AA
Cuts at: 2428 2428
Size: 4067
BstEII G'GTnAC_C
Cuts at: 468 468
Size: 4067
BstXI CCAn_nnnn'nTGG
Cuts at: 423 423
Size: 4067
BstYI r'GATC_y
Cuts at: 1 1092 1559 1582 1917 2163 3175 3261 3273
Size: 1091 467 23 335 246 1012 86 12
Cuts at: 3273 3619 1
Size: 346 449
Fragments arranged by size:
1091 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 642 642
Size: 4067
BtgI C'CryG_G
Cuts at: 158 158
Size: 4067
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 402 2017 2198 2361 402
Size: 1615 181 163 2108
Fragments arranged by size:
2108 1615 181 163
BtsI GCAGTG_nn'
Cuts at: 122 1310 122
Size: 1188 2879
DraI TTT'AAA
Cuts at: 1066 1066
Size: 4067
DrdI GACnn_nn'nnGTC
Cuts at: 795 1773 2631 795
Size: 978 858 2231
Fragments arranged by size:
2231 978 858
EaeI y'GGCC_r
Cuts at: 137 311 413 1652 1826 2217 2244 3589 137
Size: 174 102 1239 174 391 27 1345 615
Fragments arranged by size:
1345 1239 615 391 174 174 102 27
EagI C'GGCC_G
Cuts at: 1652 1652
Size: 4067
EarI CTCTTCn'nnn_
Cuts at: 2090 2300 2090
Size: 210 3857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2275 2585 2731 3877 2275
Size: 310 146 1146 2465
Fragments arranged by size:
2465 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1079 1079
Size: 4067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 630 1892 2324 3071 3339 630
Size: 1262 432 747 268 1358
Fragments arranged by size:
1358 1262 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1206 1206
Size: 4067
EcoRI G'AATT_C
Cuts at: 1053 1071 1053
Size: 18 4049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 4067
FspI TGC'GCA
Cuts at: 1848 1848
Size: 4067
HaeII r_GCGC'y
Cuts at: 1501 1749 2771 3750 1501
Size: 248 1022 979 1818
Fragments arranged by size:
1818 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1909 1939 1941 1971 1909
Size: 30 2 30 4005
Fragments arranged by size:
4005 30 30 2
HincII GTy'rAC
Cuts at: 1106 1106
Size: 4067
HindIII A'AGCT_T
Cuts at: 445 445
Size: 4067
KasI G'GCGC_C
Cuts at: 1497 1745 1497
Size: 248 3819
KpnI G_GTAC'C
Cuts at: 886 1087 886
Size: 201 3866
MluI A'CGCG_T
Cuts at: 3500 3500
Size: 4067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2738 2922 2738
Size: 184 3883
MscI TGG'CCA
Cuts at: 139 313 415 1828 139
Size: 174 102 1413 2378
Fragments arranged by size:
2378 1413 174 102
MslI CAynn'nnrTG
Cuts at: 157 211 421 584 625 2056 2183 157
Size: 54 210 163 41 1431 127 2041
Fragments arranged by size:
2041 1431 210 163 127 54 41
MspA1I CmG'CkG
Cuts at: 353 1178 1492 1852 2865 3110 353
Size: 825 314 360 1013 245 1310
Fragments arranged by size:
1310 1013 825 360 314 245
NaeI GCC'GGC
Cuts at: 2248 2248
Size: 4067
NarI GG'CG_CC
Cuts at: 1498 1746 1498
Size: 248 3819
NcoI C'CATG_G
Cuts at: 158 158
Size: 4067
NgoMIV G'CCGG_C
Cuts at: 2246 2246
Size: 4067
NspI r_CATG'y
Cuts at: 1120 1620 2527 1120
Size: 500 907 2660
Fragments arranged by size:
2660 907 500
PasI CC'CwG_GG
Cuts at: 498 498
Size: 4067
PciI A'CATG_T
Cuts at: 2523 2523
Size: 4067
PmeI GTTT'AAAC
Cuts at: 1066 1066
Size: 4067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 4035
PstI C_TGCA'G
Cuts at: 239 1114 1799 239
Size: 875 685 2507
Fragments arranged by size:
2507 875 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4067
PvuII CAG'CTG
Cuts at: 353 1492 1852 353
Size: 1139 360 2568
Fragments arranged by size:
2568 1139 360
RsrII CG'GwC_CG
Cuts at: 2262 2262
Size: 4067
SacI G_AGCT'C
Cuts at: 1081 1081
Size: 4067
SalI G'TCGA_C
Cuts at: 1104 1104
Size: 4067
SapI GCTCTTCn'nnn_
Cuts at: 2090 2300 2090
Size: 210 3857
SbfI CC_TGCA'GG
Cuts at: 1114 1114
Size: 4067
ScaI AGT'ACT
Cuts at: 3584 3584
Size: 4067
SfcI C'TryA_G
Cuts at: 33 235 348 1110 1795 2788 2979 33
Size: 202 113 762 685 993 191 1121
Fragments arranged by size:
1121 993 762 685 202 191 113
SfoI GGC'GCC
Cuts at: 1499 1747 1499
Size: 248 3819
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
SmaI CCC'GGG
Cuts at: 1089 1089
Size: 4067
SmlI C'TyrA_G
Cuts at: 1222 2137 2629 2891 1222
Size: 915 492 262 2398
Fragments arranged by size:
2398 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4067
SphI G_CATG'C
Cuts at: 1120 1620 1120
Size: 500 3567
StyI C'CwwG_G
Cuts at: 158 1201 1555 158
Size: 1043 354 2670
Fragments arranged by size:
2670 1043 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 443 1650 1783 2242 2451 443
Size: 1207 133 459 209 2059
Fragments arranged by size:
2059 1207 459 209 133
TatI w'GTAC_w
Cuts at: 899 3582 899
Size: 2683 1384
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3584 3616 3584
Size: 32 4035
Tth111I GACn'n_nGTC
Cuts at: 1864 1864
Size: 4067
XbaI T'CTAG_A
Cuts at: 1098 1098
Size: 4067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1562 1562
Size: 4067
XmaI C'CCGG_G
Cuts at: 1087 1087
Size: 4067
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsmBI BssHII Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI