Restriction map for
pF1KB5375
(Circular) (Six-base) MAPSORT of: pF1KB5375.seq Check: 845 from: 1 to: 3788
pF1KB5375 3788 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 826 826
Size: 3788
Acc65I G'GTAC_C
Cuts at: 804 804
Size: 3788
AclI AA'CG_TT
Cuts at: 3628 3628
Size: 3788
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 508 682 1613 2045 2792 3060 410
Size: 98 174 931 432 747 268 1138
Fragments arranged by size:
1138 931 747 432 268 174 98
AflIII A'CryG_T
Cuts at: 218 374 1140 2244 3221 218
Size: 156 766 1104 977 785
Fragments arranged by size:
1104 977 785 766 156
AgeI A'CCGG_T
Cuts at: 2176 2176
Size: 3788
AleI CACnn'nnGTG
Cuts at: 583 583
Size: 3788
AlwNI CAG_nnn'CTG
Cuts at: 1093 2660 1093
Size: 1567 2221
ApaLI G'TGCA_C
Cuts at: 2558 2558
Size: 3788
ApoI r'AATT_y
Cuts at: 268 550 792 3141 3397 268
Size: 282 242 2349 256 659
Fragments arranged by size:
2349 659 282 256 242
AseI AT'TA_AT
Cuts at: 20 20
Size: 3788
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
AvaI C'yCGr_G
Cuts at: 133 808 133
Size: 675 3113
BamHI G'GATC_C
Cuts at: 813 813
Size: 3788
BanI G'GyrC_C
Cuts at: 804 1218 1466 1501 804
Size: 414 248 35 3091
Fragments arranged by size:
3091 414 248 35
BanII G_rGCy'C
Cuts at: 802 1832 802
Size: 1030 2758
BbeI G_GCGC'C
Cuts at: 1222 1470 1222
Size: 248 3540
BciVI GTATCCnnnnn_n'
Cuts at: 186 1686 2447 3788 186
Size: 1500 761 1341 186
Fragments arranged by size:
1500 1341 761 186
BclI T'GATC_A
Cuts at: 720 1308 720
Size: 588 3200
BglII A'GATC_T
Cuts at: 1303 3340 1303
Size: 2037 1751
BlpI GC'TnA_GC
Cuts at: 900 900
Size: 3788
Bme1580I G_kGCm'C
Cuts at: 1413 1506 2562 1413
Size: 93 1056 2639
Fragments arranged by size:
2639 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 150 753 1000 1081 1416 3560 150
Size: 603 247 81 335 2144 378
Fragments arranged by size:
2144 603 378 335 247 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 3788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 202
Size: 3788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 964 1843 2335 2633 964
Size: 879 492 298 2119
Fragments arranged by size:
2119 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3492 3492
Size: 3788
BsaAI yAC'GTr
Cuts at: 221 1141 221
Size: 920 2868
BsaBI GATnn'nnATC
Cuts at: 6 739 1302 3339 6
Size: 733 563 2037 455
Fragments arranged by size:
2037 733 563 455
BsaHI Gr'CG_yC
Cuts at: 1219 1467 2169 3665 1219
Size: 248 702 1496 1342
Fragments arranged by size:
1496 1342 702 248
BsaWI w'CCGG_w
Cuts at: 977 1498 2176 2450 2597 3008 977
Size: 521 678 274 147 411 1757
Fragments arranged by size:
1757 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 199 229 199
Size: 30 3758
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 220
Size: 3788
BseYI C'CCAG_C
Cuts at: 1213 1971 2548 3044 1213
Size: 758 577 496 1957
Fragments arranged by size:
1957 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1330 1376 2584 104
Size: 1226 46 1208 1308
Fragments arranged by size:
1308 1226 1208 46
BsiHKAI G_wGCw'C
Cuts at: 802 1580 1770 2562 802
Size: 778 190 792 2028
Fragments arranged by size:
2028 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 802 1413 1506 1580 1770 1832 2562 802
Size: 611 93 74 190 62 730 2028
Fragments arranged by size:
2028 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3008 3008
Size: 3788
BspHI T'CATG_A
Cuts at: 2964 2964
Size: 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 838 1052 1354 1735 838
Size: 214 302 381 2891
Fragments arranged by size:
2891 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2080 2134 3618 3783 50
Size: 2030 54 1484 165 55
Fragments arranged by size:
2030 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 345 1700 345
Size: 1355 2433
BsrFI r'CCGG_y
Cuts at: 1786 1967 2176 1786
Size: 181 209 3398
Fragments arranged by size:
3398 209 181
BssSI C'ACGA_G
Cuts at: 2059 2417 2059
Size: 358 3430
BstAPI GCAn_nnn'nTGC
Cuts at: 1165 1165
Size: 3788
BstBI TT'CG_AA
Cuts at: 2149 2149
Size: 3788
BstXI CCAn_nnnn'nTGG
Cuts at: 543 642 543
Size: 99 3689
BstYI r'GATC_y
Cuts at: 1 813 1280 1303 1638 1884 2896 2982 2994
Size: 812 467 23 335 246 1012 86 12
Cuts at: 2994 3340 1
Size: 346 449
Fragments arranged by size:
1012 812 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 342 1738 1919 2082 342
Size: 1396 181 163 2048
Fragments arranged by size:
2048 1396 181 163
BtsI GCAGTG_nn'
Cuts at: 1031 1031
Size: 3788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 360 395 360
Size: 35 3753
DraI TTT'AAA
Cuts at: 787 787
Size: 3788
DrdI GACnn_nn'nnGTC
Cuts at: 1494 2352 1494
Size: 858 2930
EaeI y'GGCC_r
Cuts at: 1373 1547 1938 1965 3310 1373
Size: 174 391 27 1345 1851
Fragments arranged by size:
1851 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1373 1373
Size: 3788
EarI CTCTTCn'nnn_
Cuts at: 500 1811 2021 500
Size: 1311 210 2267
Fragments arranged by size:
2267 1311 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 160 1996 2306 2452 3598 160
Size: 1836 310 146 1146 350
Fragments arranged by size:
1836 1146 350 310 146
EcoICRI GAG'CTC
Cuts at: 800 800
Size: 3788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 410 508 682 1613 2045 2792 3060 202
Size: 208 98 174 931 432 747 268 930
Fragments arranged by size:
931 930 747 432 268 208 174 98
EcoO109I rG'GnC_Cy
Cuts at: 927 927
Size: 3788
EcoRI G'AATT_C
Cuts at: 792 792
Size: 3788
EcoRV GAT'ATC
Cuts at: 606 606
Size: 3788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 597 1807 597
Size: 1210 2578
FspI TGC'GCA
Cuts at: 1569 1569
Size: 3788
HaeII r_GCGC'y
Cuts at: 1222 1470 2492 3471 1222
Size: 248 1022 979 1539
Fragments arranged by size:
1539 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 398 430 637 669 1630 1660 1662 1692 398
Size: 32 207 32 961 30 2 30 2494
Fragments arranged by size:
2494 961 207 32 32 30 30 2
HincII GTy'rAC
Cuts at: 827 827
Size: 3788
KasI G'GCGC_C
Cuts at: 1218 1466 1218
Size: 248 3540
KpnI G_GTAC'C
Cuts at: 808 808
Size: 3788
MluI A'CGCG_T
Cuts at: 3221 3221
Size: 3788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2459 2643 2459
Size: 184 3604
MscI TGG'CCA
Cuts at: 1549 1549
Size: 3788
MslI CAynn'nnrTG
Cuts at: 583 1777 1904 583
Size: 1194 127 2467
Fragments arranged by size:
2467 1194 127
MspA1I CmG'CkG
Cuts at: 899 1213 1573 2586 2831 899
Size: 314 360 1013 245 1856
Fragments arranged by size:
1856 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1969 1969
Size: 3788
NarI GG'CG_CC
Cuts at: 1219 1467 1219
Size: 248 3540
NgoMIV G'CCGG_C
Cuts at: 1967 1967
Size: 3788
NspI r_CATG'y
Cuts at: 378 841 1341 2248 378
Size: 463 500 907 1918
Fragments arranged by size:
1918 907 500 463
PciI A'CATG_T
Cuts at: 374 2244 374
Size: 1870 1918
PflMI CCAn_nnn'nTGG
Cuts at: 258 432 258
Size: 174 3614
PfoI T'CCnGG_A
Cuts at: 179 308 179
Size: 129 3659
PmeI GTTT'AAAC
Cuts at: 787 787
Size: 3788
PmlI CAC'GTG
Cuts at: 221 221
Size: 3788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2927 2959 2927
Size: 32 3756
PstI C_TGCA'G
Cuts at: 835 1520 835
Size: 685 3103
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3788
PvuII CAG'CTG
Cuts at: 1213 1573 1213
Size: 360 3428
RsrII CG'GwC_CG
Cuts at: 1983 1983
Size: 3788
SacI G_AGCT'C
Cuts at: 802 802
Size: 3788
SalI G'TCGA_C
Cuts at: 825 825
Size: 3788
SapI GCTCTTCn'nnn_
Cuts at: 500 1811 2021 500
Size: 1311 210 2267
Fragments arranged by size:
2267 1311 210
SbfI CC_TGCA'GG
Cuts at: 835 835
Size: 3788
ScaI AGT'ACT
Cuts at: 499 3305 499
Size: 2806 982
SfcI C'TryA_G
Cuts at: 33 831 1516 2509 2700 33
Size: 798 685 993 191 1121
Fragments arranged by size:
1121 993 798 685 191
SfoI GGC'GCC
Cuts at: 1220 1468 1220
Size: 248 3540
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
SmaI CCC'GGG
Cuts at: 810 810
Size: 3788
SmlI C'TyrA_G
Cuts at: 943 1858 2350 2612 943
Size: 915 492 262 2119
Fragments arranged by size:
2119 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3788
SphI G_CATG'C
Cuts at: 841 1341 841
Size: 500 3288
StyI C'CwwG_G
Cuts at: 922 1276 922
Size: 354 3434
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 346 1371 1504 1963 2172 346
Size: 1025 133 459 209 1962
Fragments arranged by size:
1962 1025 459 209 133
TatI w'GTAC_w
Cuts at: 497 3303 497
Size: 2806 982
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 3756
Tth111I GACn'n_nGTC
Cuts at: 1585 1585
Size: 3788
XbaI T'CTAG_A
Cuts at: 819 819
Size: 3788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1283 1283
Size: 3788
XmaI C'CCGG_G
Cuts at: 808 808
Size: 3788
XmnI GAAnn'nnTTC
Cuts at: 401 529 667 401
Size: 128 138 3522
Fragments arranged by size:
3522 138 128
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI DraIII EcoNI
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI