Restriction map for
pF1KB5364
(Circular) (Six-base) MAPSORT of: pF1KB5364.seq Check: 3732 from: 1 to: 4298
pF1KB5364 4298 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 381 381
Size: 4298
AccI GT'mk_AC
Cuts at: 1214 1336 1214
Size: 122 4176
Acc65I G'GTAC_C
Cuts at: 1314 1314
Size: 4298
AclI AA'CG_TT
Cuts at: 783 4138 783
Size: 3355 943
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 367 2123 2555 3302 3570 206
Size: 161 1756 432 747 268 934
Fragments arranged by size:
1756 934 747 432 268 161
AflIII A'CryG_T
Cuts at: 1650 2754 3731 1650
Size: 1104 977 2217
Fragments arranged by size:
2217 1104 977
AgeI A'CCGG_T
Cuts at: 2686 2686
Size: 4298
AlwNI CAG_nnn'CTG
Cuts at: 1603 3170 1603
Size: 1567 2731
ApaLI G'TGCA_C
Cuts at: 3068 3068
Size: 4298
ApoI r'AATT_y
Cuts at: 339 669 909 1302 3651 3907 339
Size: 330 240 393 2349 256 730
Fragments arranged by size:
2349 730 393 330 256 240
AseI AT'TA_AT
Cuts at: 20 20
Size: 4298
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
AvaI C'yCGr_G
Cuts at: 1318 1318
Size: 4298
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 620 653 620
Size: 33 4265
BamHI G'GATC_C
Cuts at: 1323 1323
Size: 4298
BanI G'GyrC_C
Cuts at: 315 1314 1728 1976 2011 315
Size: 999 414 248 35 2602
Fragments arranged by size:
2602 999 414 248 35
BanII G_rGCy'C
Cuts at: 1312 2342 1312
Size: 1030 3268
BbeI G_GCGC'C
Cuts at: 1732 1980 1732
Size: 248 4050
BbsI GAAGACnn'nnnn_
Cuts at: 560 693 560
Size: 133 4165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4264
BciVI GTATCCnnnnn_n'
Cuts at: 2196 2957 4298 2196
Size: 761 1341 2196
Fragments arranged by size:
2196 1341 761
BclI T'GATC_A
Cuts at: 1818 1818
Size: 4298
BglI GCCn_nnn'nGGC
Cuts at: 422 422
Size: 4298
BglII A'GATC_T
Cuts at: 1813 3850 1813
Size: 2037 2261
BlpI GC'TnA_GC
Cuts at: 1034 1410 1034
Size: 376 3922
Bme1580I G_kGCm'C
Cuts at: 1923 2016 3072 1923
Size: 93 1056 3149
Fragments arranged by size:
3149 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1510 1591 1926 4070 1510
Size: 81 335 2144 1738
Fragments arranged by size:
2144 1738 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1170 2665 1170
Size: 1495 2803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1474 2353 2845 3143 1474
Size: 879 492 298 2629
Fragments arranged by size:
2629 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4002 4002
Size: 4298
BsaAI yAC'GTr
Cuts at: 1651 1651
Size: 4298
BsaBI GATnn'nnATC
Cuts at: 6 1812 3849 6
Size: 1806 2037 455
Fragments arranged by size:
2037 1806 455
BsaHI Gr'CG_yC
Cuts at: 1729 1977 2679 4175 1729
Size: 248 702 1496 1852
Fragments arranged by size:
1852 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1487 2008 2686 2960 3107 3518 1487
Size: 521 678 274 147 411 2267
Fragments arranged by size:
2267 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 201 231 201
Size: 30 4268
BseYI C'CCAG_C
Cuts at: 939 1723 2481 3058 3554 939
Size: 784 758 577 496 1683
Fragments arranged by size:
1683 784 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 263
Size: 4298
BsiEI CG_ry'CG
Cuts at: 104 1840 1886 3094 104
Size: 1736 46 1208 1308
Fragments arranged by size:
1736 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1312 2090 2280 3072 1312
Size: 778 190 792 2538
Fragments arranged by size:
2538 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1312 1923 2016 2090 2280 2342 3072 1312
Size: 611 93 74 190 62 730 2538
Fragments arranged by size:
2538 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3518 3518
Size: 4298
BspHI T'CATG_A
Cuts at: 3474 3474
Size: 4298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 381 1268 1348 1562 1864 2245 381
Size: 887 80 214 302 381 2434
Fragments arranged by size:
2434 887 381 302 214 80
BsrBI CCG'CTC
Cuts at: 50 2590 2644 4128 4293 50
Size: 2540 54 1484 165 55
Fragments arranged by size:
2540 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2210 2210
Size: 4298
BsrFI r'CCGG_y
Cuts at: 2296 2477 2686 2296
Size: 181 209 3908
Fragments arranged by size:
3908 209 181
BssSI C'ACGA_G
Cuts at: 992 2569 2927 992
Size: 1577 358 2363
Fragments arranged by size:
2363 1577 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1675 1675
Size: 4298
BstBI TT'CG_AA
Cuts at: 1250 2659 1250
Size: 1409 2889
BstXI CCAn_nnnn'nTGG
Cuts at: 933 933
Size: 4298
BstYI r'GATC_y
Cuts at: 1 1323 1790 1813 2148 2394 3406 3492 3504
Size: 1322 467 23 335 246 1012 86 12
Cuts at: 3504 3850 1
Size: 346 449
Fragments arranged by size:
1322 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1215 1215
Size: 4298
BtgI C'CryG_G
Cuts at: 184 250 184
Size: 66 4232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2248 2429 2592 2248
Size: 181 163 3954
Fragments arranged by size:
3954 181 163
BtsI GCAGTG_nn'
Cuts at: 1541 1541
Size: 4298
DraI TTT'AAA
Cuts at: 1297 1297
Size: 4298
DrdI GACnn_nn'nnGTC
Cuts at: 764 2004 2862 764
Size: 1240 858 2200
Fragments arranged by size:
2200 1240 858
EaeI y'GGCC_r
Cuts at: 1883 2057 2448 2475 3820 1883
Size: 174 391 27 1345 2361
Fragments arranged by size:
2361 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1883 1883
Size: 4298
EarI CTCTTCn'nnn_
Cuts at: 440 2321 2531 440
Size: 1881 210 2207
Fragments arranged by size:
2207 1881 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2506 2816 2962 4108 2506
Size: 310 146 1146 2696
Fragments arranged by size:
2696 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1310 1310
Size: 4298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 367 2123 2555 3302 3570 206
Size: 161 1756 432 747 268 934
Fragments arranged by size:
1756 934 747 432 268 161
EcoNI CCTnn'n_nnAGG
Cuts at: 221 1283 221
Size: 1062 3236
EcoO109I rG'GnC_Cy
Cuts at: 1437 1437
Size: 4298
EcoRI G'AATT_C
Cuts at: 339 1302 339
Size: 963 3335
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 4298
FspI TGC'GCA
Cuts at: 2079 2079
Size: 4298
HaeII r_GCGC'y
Cuts at: 1732 1980 3002 3981 1732
Size: 248 1022 979 2049
Fragments arranged by size:
2049 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1165 1197 2140 2170 2172 2202 1165
Size: 32 943 30 2 30 3261
Fragments arranged by size:
3261 943 32 30 30 2
HincII GTy'rAC
Cuts at: 740 1184 1337 740
Size: 444 153 3701
Fragments arranged by size:
3701 444 153
HindIII A'AGCT_T
Cuts at: 714 714
Size: 4298
HpaI GTT'AAC
Cuts at: 740 740
Size: 4298
KasI G'GCGC_C
Cuts at: 1728 1976 1728
Size: 248 4050
KpnI G_GTAC'C
Cuts at: 1318 1318
Size: 4298
MluI A'CGCG_T
Cuts at: 3731 3731
Size: 4298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1057 2969 3153 1057
Size: 1912 184 2202
Fragments arranged by size:
2202 1912 184
MscI TGG'CCA
Cuts at: 2059 2059
Size: 4298
MslI CAynn'nnrTG
Cuts at: 2287 2414 2287
Size: 127 4171
MspA1I CmG'CkG
Cuts at: 158 469 1409 1723 2083 3096 3341 158
Size: 311 940 314 360 1013 245 1115
Fragments arranged by size:
1115 1013 940 360 314 311 245
NaeI GCC'GGC
Cuts at: 2479 2479
Size: 4298
NarI GG'CG_CC
Cuts at: 1729 1977 1729
Size: 248 4050
NcoI C'CATG_G
Cuts at: 250 250
Size: 4298
NgoMIV G'CCGG_C
Cuts at: 2477 2477
Size: 4298
NspI r_CATG'y
Cuts at: 738 1351 1851 2758 738
Size: 613 500 907 2278
Fragments arranged by size:
2278 907 613 500
PciI A'CATG_T
Cuts at: 2754 2754
Size: 4298
PmeI GTTT'AAAC
Cuts at: 1297 1297
Size: 4298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 230 262 3437 3469 230
Size: 32 3175 32 1059
Fragments arranged by size:
3175 1059 32 32
PstI C_TGCA'G
Cuts at: 202 1345 2030 202
Size: 1143 685 2470
Fragments arranged by size:
2470 1143 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4298
PvuII CAG'CTG
Cuts at: 158 1723 2083 158
Size: 1565 360 2373
Fragments arranged by size:
2373 1565 360
RsrII CG'GwC_CG
Cuts at: 2493 2493
Size: 4298
SacI G_AGCT'C
Cuts at: 1312 1312
Size: 4298
SalI G'TCGA_C
Cuts at: 1335 1335
Size: 4298
SapI GCTCTTCn'nnn_
Cuts at: 2321 2531 2321
Size: 210 4088
SbfI CC_TGCA'GG
Cuts at: 1345 1345
Size: 4298
ScaI AGT'ACT
Cuts at: 3815 3815
Size: 4298
SfcI C'TryA_G
Cuts at: 33 198 1341 2026 3019 3210 33
Size: 165 1143 685 993 191 1121
Fragments arranged by size:
1143 1121 993 685 191 165
SfoI GGC'GCC
Cuts at: 1730 1978 1730
Size: 248 4050
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
SmaI CCC'GGG
Cuts at: 1320 1320
Size: 4298
SmlI C'TyrA_G
Cuts at: 1453 2368 2860 3122 1453
Size: 915 492 262 2629
Fragments arranged by size:
2629 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4298
SphI G_CATG'C
Cuts at: 1351 1851 1351
Size: 500 3798
SspI AAT'ATT
Cuts at: 510 510
Size: 4298
StyI C'CwwG_G
Cuts at: 250 1432 1786 250
Size: 1182 354 2762
Fragments arranged by size:
2762 1182 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 599 1881 2014 2473 2682 599
Size: 1282 133 459 209 2215
Fragments arranged by size:
2215 1282 459 209 133
TatI w'GTAC_w
Cuts at: 535 3813 535
Size: 3278 1020
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3815 3847 3815
Size: 32 4266
Tth111I GACn'n_nGTC
Cuts at: 2095 2095
Size: 4298
XbaI T'CTAG_A
Cuts at: 816 1329 816
Size: 513 3785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1793 1793
Size: 4298
XmaI C'CCGG_G
Cuts at: 1318 1318
Size: 4298
XmnI GAAnn'nnTTC
Cuts at: 824 824
Size: 4298
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BfrBI BmgBI BmtI BpmI Bpu10I
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI