Restriction map for
pF1KB5349
(Circular) (Six-base) MAPSORT of: pF1KB5349.seq Check: 9650 from: 1 to: 4241
pF1KB5349 4241 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 1279 1279
Size: 4241
Acc65I G'GTAC_C
Cuts at: 1257 1257
Size: 4241
AclI AA'CG_TT
Cuts at: 4081 4081
Size: 4241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 880 2066 2498 3245 3513 208
Size: 672 1186 432 747 268 936
Fragments arranged by size:
1186 936 747 672 432 268
AflIII A'CryG_T
Cuts at: 614 1593 2697 3674 614
Size: 979 1104 977 1181
Fragments arranged by size:
1181 1104 979 977
AgeI A'CCGG_T
Cuts at: 2629 2629
Size: 4241
AleI CACnn'nnGTG
Cuts at: 1183 1183
Size: 4241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1216 1216
Size: 4241
AlwNI CAG_nnn'CTG
Cuts at: 679 804 858 912 1546 3113 679
Size: 125 54 54 634 1567 1807
Fragments arranged by size:
1807 1567 634 125 54 54
ApaI G_GGCC'C
Cuts at: 155 155
Size: 4241
ApaLI G'TGCA_C
Cuts at: 1176 3011 1176
Size: 1835 2406
ApoI r'AATT_y
Cuts at: 304 920 1245 3594 3850 304
Size: 616 325 2349 256 695
Fragments arranged by size:
2349 695 616 325 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4241
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 154 1121 1261 154
Size: 967 140 3134
Fragments arranged by size:
3134 967 140
BamHI G'GATC_C
Cuts at: 246 1266 246
Size: 1020 3221
BanI G'GyrC_C
Cuts at: 173 1257 1671 1919 1954 173
Size: 1084 414 248 35 2460
Fragments arranged by size:
2460 1084 414 248 35
BanII G_rGCy'C
Cuts at: 148 155 191 468 1255 2285 148
Size: 7 36 277 787 1030 2104
Fragments arranged by size:
2104 1030 787 277 36 7
BbeI G_GCGC'C
Cuts at: 1675 1923 1675
Size: 248 3993
BbsI GAAGACnn'nnnn_
Cuts at: 868 868
Size: 4241
BciVI GTATCCnnnnn_n'
Cuts at: 2139 2900 4241 2139
Size: 761 1341 2139
Fragments arranged by size:
2139 1341 761
BclI T'GATC_A
Cuts at: 1761 1761
Size: 4241
BglII A'GATC_T
Cuts at: 1756 3793 1756
Size: 2037 2204
BlpI GC'TnA_GC
Cuts at: 1353 1353
Size: 4241
Bme1580I G_kGCm'C
Cuts at: 155 1180 1866 1959 3015 155
Size: 1025 686 93 1056 1381
Fragments arranged by size:
1381 1056 1025 686 93
BmrI ACTGGGnnnn_n'
Cuts at: 1453 1534 1869 4013 1453
Size: 81 335 2144 1681
Fragments arranged by size:
2144 1681 335 81
BmtI G_CTAG'C
Cuts at: 715 715
Size: 4241
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 289 772 2608 289
Size: 483 1836 1922
Fragments arranged by size:
1922 1836 483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 567 793 1186 202
Size: 365 226 393 3257
Fragments arranged by size:
3257 393 365 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1417 2296 2788 3086 1417
Size: 879 492 298 2572
Fragments arranged by size:
2572 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 277 3945 277
Size: 3668 573
BsaAI yAC'GTr
Cuts at: 354 1594 354
Size: 1240 3001
BsaBI GATnn'nnATC
Cuts at: 6 1755 3792 6
Size: 1749 2037 455
Fragments arranged by size:
2037 1749 455
BsaHI Gr'CG_yC
Cuts at: 1672 1920 2622 4118 1672
Size: 248 702 1496 1795
Fragments arranged by size:
1795 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1430 1951 2629 2903 3050 3461 1430
Size: 521 678 274 147 411 2210
Fragments arranged by size:
2210 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1159 1189 1159
Size: 30 4211
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 745 199
Size: 546 3695
BseYI C'CCAG_C
Cuts at: 705 905 1666 2424 3001 3497 705
Size: 200 761 758 577 496 1449
Fragments arranged by size:
1449 761 758 577 496 200
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 1135 262
Size: 873 3368
BsiEI CG_ry'CG
Cuts at: 104 1783 1829 3037 104
Size: 1679 46 1208 1308
Fragments arranged by size:
1679 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 148 191 468 1180 1214 1255 2033 2223 3015
Size: 43 277 712 34 41 778 190 792
Cuts at: 3015 148
Size: 1374
Fragments arranged by size:
1374 792 778 712 277 190 43 41
34
Bsp1286I G_dGCh'C
Cuts at: 148 155 191 468 1180 1214 1255 1866 1959
Size: 7 36 277 712 34 41 611 93
Cuts at: 1959 2033 2223 2285 3015 148
Size: 74 190 62 730 1374
Fragments arranged by size:
1374 730 712 611 277 190 93 74
62 41 36 34 7
BspEI T'CCGG_A
Cuts at: 3461 3461
Size: 4241
BspHI T'CATG_A
Cuts at: 3417 3417
Size: 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 969 1291 1505 1807 2188 969
Size: 322 214 302 381 3022
Fragments arranged by size:
3022 381 322 302 214
BsrBI CCG'CTC
Cuts at: 50 846 2533 2587 4071 4236 50
Size: 796 1687 54 1484 165 55
Fragments arranged by size:
1687 1484 796 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2153 2153
Size: 4241
BsrFI r'CCGG_y
Cuts at: 794 2239 2420 2629 794
Size: 1445 181 209 2406
Fragments arranged by size:
2406 1445 209 181
BssSI C'ACGA_G
Cuts at: 334 407 2512 2870 334
Size: 73 2105 358 1705
Fragments arranged by size:
2105 1705 358 73
BstAPI GCAn_nnn'nTGC
Cuts at: 768 1618 768
Size: 850 3391
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstXI CCAn_nnnn'nTGG
Cuts at: 268 268
Size: 4241
BstYI r'GATC_y
Cuts at: 1 246 666 1266 1733 1756 2091 2337 3349
Size: 245 420 600 467 23 335 246 1012
Cuts at: 3349 3435 3447 3793 1
Size: 86 12 346 449
Fragments arranged by size:
1012 600 467 449 420 346 335 246
245 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 781 781
Size: 4241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2191 2372 2535 2191
Size: 181 163 3897
Fragments arranged by size:
3897 181 163
BtsI GCAGTG_nn'
Cuts at: 443 805 1484 443
Size: 362 679 3200
Fragments arranged by size:
3200 679 362
DraI TTT'AAA
Cuts at: 1240 1240
Size: 4241
DraIII CAC_nnn'GTG
Cuts at: 318 768 318
Size: 450 3791
DrdI GACnn_nn'nnGTC
Cuts at: 1096 1947 2805 1096
Size: 851 858 2532
Fragments arranged by size:
2532 858 851
EaeI y'GGCC_r
Cuts at: 1826 2000 2391 2418 3763 1826
Size: 174 391 27 1345 2304
Fragments arranged by size:
2304 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1826 1826
Size: 4241
EarI CTCTTCn'nnn_
Cuts at: 1183 2264 2474 1183
Size: 1081 210 2950
Fragments arranged by size:
2950 1081 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2449 2759 2905 4051 2449
Size: 310 146 1146 2639
Fragments arranged by size:
2639 1146 310 146
EcoICRI GAG'CTC
Cuts at: 146 189 466 1253 146
Size: 43 277 787 3134
Fragments arranged by size:
3134 787 277 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 208 567 793 880 1186 2066 2498 3245
Size: 6 359 226 87 306 880 432 747
Cuts at: 3245 3513 202
Size: 268 930
Fragments arranged by size:
930 880 747 432 359 306 268 226
87 6
EcoO109I rG'GnC_Cy
Cuts at: 486 1047 1380 486
Size: 561 333 3347
Fragments arranged by size:
3347 561 333
EcoRI G'AATT_C
Cuts at: 1245 1245
Size: 4241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 925 2260 925
Size: 1335 2906
FspI TGC'GCA
Cuts at: 2022 2022
Size: 4241
HaeII r_GCGC'y
Cuts at: 1675 1923 2945 3924 1675
Size: 248 1022 979 1992
Fragments arranged by size:
1992 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 357 389 2083 2113 2115 2145 357
Size: 32 1694 30 2 30 2453
Fragments arranged by size:
2453 1694 32 30 30 2
HincII GTy'rAC
Cuts at: 657 1280 657
Size: 623 3618
HindIII A'AGCT_T
Cuts at: 280 280
Size: 4241
KasI G'GCGC_C
Cuts at: 1671 1919 1671
Size: 248 3993
KpnI G_GTAC'C
Cuts at: 1261 1261
Size: 4241
MfeI C'AATT_G
Cuts at: 458 509 458
Size: 51 4190
MluI A'CGCG_T
Cuts at: 614 3674 614
Size: 3060 1181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1109 2912 3096 1109
Size: 1803 184 2254
Fragments arranged by size:
2254 1803 184
MscI TGG'CCA
Cuts at: 2002 2002
Size: 4241
MslI CAynn'nnrTG
Cuts at: 943 1183 1198 2230 2357 943
Size: 240 15 1032 127 2827
Fragments arranged by size:
2827 1032 240 127 15
MspA1I CmG'CkG
Cuts at: 166 216 1352 1666 2026 3039 3284 166
Size: 50 1136 314 360 1013 245 1123
Fragments arranged by size:
1136 1123 1013 360 314 245 50
NaeI GCC'GGC
Cuts at: 2422 2422
Size: 4241
NarI GG'CG_CC
Cuts at: 1672 1920 1672
Size: 248 3993
NgoMIV G'CCGG_C
Cuts at: 2420 2420
Size: 4241
NheI G'CTAG_C
Cuts at: 711 711
Size: 4241
NspI r_CATG'y
Cuts at: 1294 1794 2701 1294
Size: 500 907 2834
Fragments arranged by size:
2834 907 500
PciI A'CATG_T
Cuts at: 2697 2697
Size: 4241
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 3380
Size: 32 4209
PpuMI rG'GwC_Cy
Cuts at: 486 486
Size: 4241
PspOMI G'GGCC_C
Cuts at: 151 151
Size: 4241
PstI C_TGCA'G
Cuts at: 1288 1973 1288
Size: 685 3556
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4241
PvuII CAG'CTG
Cuts at: 216 1666 2026 216
Size: 1450 360 2431
Fragments arranged by size:
2431 1450 360
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 148 191 468 1255 148
Size: 43 277 787 3134
Fragments arranged by size:
3134 787 277 43
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 1183 2264 2474 1183
Size: 1081 210 2950
Fragments arranged by size:
2950 1081 210
SbfI CC_TGCA'GG
Cuts at: 1288 1288
Size: 4241
ScaI AGT'ACT
Cuts at: 3758 3758
Size: 4241
SfcI C'TryA_G
Cuts at: 33 130 490 1284 1969 2962 3153 33
Size: 97 360 794 685 993 191 1121
Fragments arranged by size:
1121 993 794 685 360 191 97
SfoI GGC'GCC
Cuts at: 1673 1921 1673
Size: 248 3993
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SmaI CCC'GGG
Cuts at: 156 1263 156
Size: 1107 3134
SmlI C'TyrA_G
Cuts at: 1396 2311 2803 3065 1396
Size: 915 492 262 2572
Fragments arranged by size:
2572 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4241
SphI G_CATG'C
Cuts at: 1294 1794 1294
Size: 500 3741
SrfI GCCC'GGGC
Cuts at: 156 156
Size: 4241
StyI C'CwwG_G
Cuts at: 261 1375 1729 261
Size: 1114 354 2773
Fragments arranged by size:
2773 1114 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1101 1824 1957 2416 2625 1101
Size: 723 133 459 209 2717
Fragments arranged by size:
2717 723 459 209 133
TatI w'GTAC_w
Cuts at: 3756 3756
Size: 4241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1159 1191 3758 3790 1159
Size: 32 2567 32 1610
Fragments arranged by size:
2567 1610 32 32
Tth111I GACn'n_nGTC
Cuts at: 2038 2038
Size: 4241
XbaI T'CTAG_A
Cuts at: 1272 1272
Size: 4241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1736 1736
Size: 4241
XmaI C'CCGG_G
Cuts at: 154 1261 154
Size: 1107 3134
XmnI GAAnn'nnTTC
Cuts at: 344 344
Size: 4241
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NspI PciI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI Bpu10I BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I BtgI ClaI
CspCI EcoNI EcoRV FseI FspAI HpaI NcoI NdeI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SspI StuI SwaI XhoI ZraI