Restriction map for
pF1KB5337
(Circular) (Six-base) MAPSORT of: pF1KB5337.seq Check: 7123 from: 1 to: 3887
pF1KB5337 3887 bp
With 182 enzymes: SgfI *
February 20, 2009 16:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 506 506
Size: 3887
AccI GT'mk_AC
Cuts at: 925 925
Size: 3887
Acc65I G'GTAC_C
Cuts at: 903 903
Size: 3887
AclI AA'CG_TT
Cuts at: 3727 3727
Size: 3887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 474 1712 2144 2891 3159 474
Size: 1238 432 747 268 1202
Fragments arranged by size:
1238 1202 747 432 268
AflIII A'CryG_T
Cuts at: 1239 2343 3320 1239
Size: 1104 977 1806
Fragments arranged by size:
1806 1104 977
AgeI A'CCGG_T
Cuts at: 2275 2275
Size: 3887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 538 538
Size: 3887
AlwNI CAG_nnn'CTG
Cuts at: 497 1192 2759 497
Size: 695 1567 1625
Fragments arranged by size:
1625 1567 695
ApaI G_GGCC'C
Cuts at: 155 155
Size: 3887
ApaLI G'TGCA_C
Cuts at: 2657 2657
Size: 3887
ApoI r'AATT_y
Cuts at: 542 891 3240 3496 542
Size: 349 2349 256 933
Fragments arranged by size:
2349 933 349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3887
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
AvaI C'yCGr_G
Cuts at: 154 333 356 593 907 154
Size: 179 23 237 314 3134
Fragments arranged by size:
3134 314 237 179 23
BamHI G'GATC_C
Cuts at: 912 912
Size: 3887
BanI G'GyrC_C
Cuts at: 518 588 903 1317 1565 1600 518
Size: 70 315 414 248 35 2805
Fragments arranged by size:
2805 414 315 248 70 35
BanII G_rGCy'C
Cuts at: 155 625 901 1931 155
Size: 470 276 1030 2111
Fragments arranged by size:
2111 1030 470 276
BbeI G_GCGC'C
Cuts at: 592 1321 1569 592
Size: 729 248 2910
Fragments arranged by size:
2910 729 248
BbsI GAAGACnn'nnnn_
Cuts at: 808 808
Size: 3887
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3853
BciVI GTATCCnnnnn_n'
Cuts at: 1785 2546 3887 1785
Size: 761 1341 1785
Fragments arranged by size:
1785 1341 761
BclI T'GATC_A
Cuts at: 1407 1407
Size: 3887
BglII A'GATC_T
Cuts at: 1402 3439 1402
Size: 2037 1850
BlpI GC'TnA_GC
Cuts at: 999 999
Size: 3887
Bme1580I G_kGCm'C
Cuts at: 155 507 617 1512 1605 2661 155
Size: 352 110 895 93 1056 1381
Fragments arranged by size:
1381 1056 895 352 110 93
BmrI ACTGGGnnnn_n'
Cuts at: 1099 1180 1515 3659 1099
Size: 81 335 2144 1327
Fragments arranged by size:
2144 1327 335 81
BmtI G_CTAG'C
Cuts at: 214 214
Size: 3887
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 3887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 850 673
Size: 177 3710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1942 2434 2732 1063
Size: 879 492 298 2218
Fragments arranged by size:
2218 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 542 3591 542
Size: 3049 838
BsaAI yAC'GTr
Cuts at: 1240 1240
Size: 3887
BsaBI GATnn'nnATC
Cuts at: 6 1401 3438 6
Size: 1395 2037 455
Fragments arranged by size:
2037 1395 455
BsaHI Gr'CG_yC
Cuts at: 589 1318 1566 2268 3764 589
Size: 729 248 702 1496 712
Fragments arranged by size:
1496 729 712 702 248
BsaWI w'CCGG_w
Cuts at: 551 797 1076 1597 2275 2549 2696 3107 551
Size: 246 279 521 678 274 147 411 1331
Fragments arranged by size:
1331 678 521 411 279 274 246 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 546 576 546
Size: 30 3857
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 256 217
Size: 39 3848
BseYI C'CCAG_C
Cuts at: 756 1312 2070 2647 3143 756
Size: 556 758 577 496 1500
Fragments arranged by size:
1500 758 577 556 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 625 361
Size: 264 3623
BsiEI CG_ry'CG
Cuts at: 104 723 1429 1475 2683 104
Size: 619 706 46 1208 1308
Fragments arranged by size:
1308 1208 706 619 46
BsiHKAI G_wGCw'C
Cuts at: 559 901 1679 1869 2661 559
Size: 342 778 190 792 1785
Fragments arranged by size:
1785 792 778 342 190
Bsp1286I G_dGCh'C
Cuts at: 155 507 559 617 625 901 1512 1605 1679
Size: 352 52 58 8 276 611 93 74
Cuts at: 1679 1869 1931 2661 155
Size: 190 62 730 1381
Fragments arranged by size:
1381 730 611 352 276 190 93 74
62 58 52 8
BspEI T'CCGG_A
Cuts at: 3107 3107
Size: 3887
BspHI T'CATG_A
Cuts at: 3063 3063
Size: 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 506 937 1151 1453 1834 506
Size: 431 214 302 381 2559
Fragments arranged by size:
2559 431 381 302 214
BsrBI CCG'CTC
Cuts at: 50 657 2179 2233 3717 3882 50
Size: 607 1522 54 1484 165 55
Fragments arranged by size:
1522 1484 607 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1799 1799
Size: 3887
BsrFI r'CCGG_y
Cuts at: 1885 2066 2275 1885
Size: 181 209 3497
Fragments arranged by size:
3497 209 181
BsrGI T'GTAC_A
Cuts at: 314 314
Size: 3887
BssHII G'CGCG_C
Cuts at: 214 214
Size: 3887
BssSI C'ACGA_G
Cuts at: 124 685 2158 2516 124
Size: 561 1473 358 1495
Fragments arranged by size:
1495 1473 561 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1264 1264
Size: 3887
BstBI TT'CG_AA
Cuts at: 2248 2248
Size: 3887
BstEII G'GTnAC_C
Cuts at: 469 469
Size: 3887
BstYI r'GATC_y
Cuts at: 1 912 1379 1402 1737 1983 2995 3081 3093
Size: 911 467 23 335 246 1012 86 12
Cuts at: 3093 3439 1
Size: 346 449
Fragments arranged by size:
1012 911 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 230 230
Size: 3887
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1837 2018 2181 1837
Size: 181 163 3543
Fragments arranged by size:
3543 181 163
BtsI GCAGTG_nn'
Cuts at: 183 754 1130 183
Size: 571 376 2940
Fragments arranged by size:
2940 571 376
DraI TTT'AAA
Cuts at: 886 886
Size: 3887
DrdI GACnn_nn'nnGTC
Cuts at: 1593 2451 1593
Size: 858 3029
EaeI y'GGCC_r
Cuts at: 128 143 662 1472 1646 2037 2064 3409 128
Size: 15 519 810 174 391 27 1345 606
Fragments arranged by size:
1345 810 606 519 391 174 27 15
EagI C'GGCC_G
Cuts at: 1472 1472
Size: 3887
EarI CTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 433 2095 2405 2551 3697 433
Size: 1662 310 146 1146 623
Fragments arranged by size:
1662 1146 623 310 146
EcoICRI GAG'CTC
Cuts at: 899 899
Size: 3887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 474 673 850 1712 2144 2891 3159 474
Size: 199 177 862 432 747 268 1202
Fragments arranged by size:
1202 862 747 432 268 199 177
EcoO109I rG'GnC_Cy
Cuts at: 690 825 1026 690
Size: 135 201 3551
Fragments arranged by size:
3551 201 135
EcoRI G'AATT_C
Cuts at: 891 891
Size: 3887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1906 1906
Size: 3887
FspI TGC'GCA
Cuts at: 1668 1668
Size: 3887
HaeII r_GCGC'y
Cuts at: 592 652 1321 1569 2591 3570 592
Size: 60 669 248 1022 979 909
Fragments arranged by size:
1022 979 909 669 248 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1729 1759 1761 1791 1729
Size: 30 2 30 3825
Fragments arranged by size:
3825 30 30 2
HincII GTy'rAC
Cuts at: 926 926
Size: 3887
HindIII A'AGCT_T
Cuts at: 814 814
Size: 3887
KasI G'GCGC_C
Cuts at: 588 1317 1565 588
Size: 729 248 2910
Fragments arranged by size:
2910 729 248
KpnI G_GTAC'C
Cuts at: 907 907
Size: 3887
MluI A'CGCG_T
Cuts at: 3320 3320
Size: 3887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2558 2742 2558
Size: 184 3703
MscI TGG'CCA
Cuts at: 1648 1648
Size: 3887
MslI CAynn'nnrTG
Cuts at: 385 1876 2003 385
Size: 1491 127 2269
Fragments arranged by size:
2269 1491 127
MspA1I CmG'CkG
Cuts at: 861 998 1312 1672 2685 2930 861
Size: 137 314 360 1013 245 1818
Fragments arranged by size:
1818 1013 360 314 245 137
NaeI GCC'GGC
Cuts at: 2068 2068
Size: 3887
NarI GG'CG_CC
Cuts at: 589 1318 1566 589
Size: 729 248 2910
Fragments arranged by size:
2910 729 248
NgoMIV G'CCGG_C
Cuts at: 2066 2066
Size: 3887
NheI G'CTAG_C
Cuts at: 210 210
Size: 3887
NspI r_CATG'y
Cuts at: 940 1440 2347 940
Size: 500 907 2480
Fragments arranged by size:
2480 907 500
PasI CC'CwG_GG
Cuts at: 288 618 288
Size: 330 3557
PciI A'CATG_T
Cuts at: 2343 2343
Size: 3887
PflMI CCAn_nnn'nTGG
Cuts at: 861 861
Size: 3887
PfoI T'CCnGG_A
Cuts at: 434 434
Size: 3887
PmeI GTTT'AAAC
Cuts at: 886 886
Size: 3887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3026 3058 3026
Size: 32 3855
PpuMI rG'GwC_Cy
Cuts at: 690 690
Size: 3887
PspOMI G'GGCC_C
Cuts at: 151 151
Size: 3887
PstI C_TGCA'G
Cuts at: 449 527 934 1619 449
Size: 78 407 685 2717
Fragments arranged by size:
2717 685 407 78
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3887
PvuII CAG'CTG
Cuts at: 861 1312 1672 861
Size: 451 360 3076
Fragments arranged by size:
3076 451 360
RsrII CG'GwC_CG
Cuts at: 2082 2082
Size: 3887
SacI G_AGCT'C
Cuts at: 901 901
Size: 3887
SalI G'TCGA_C
Cuts at: 924 924
Size: 3887
SapI GCTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
SbfI CC_TGCA'GG
Cuts at: 934 934
Size: 3887
ScaI AGT'ACT
Cuts at: 3404 3404
Size: 3887
SfcI C'TryA_G
Cuts at: 33 445 498 523 930 1615 2608 2799 33
Size: 412 53 25 407 685 993 191 1121
Fragments arranged by size:
1121 993 685 412 407 191 53 25
SfoI GGC'GCC
Cuts at: 590 1319 1567 590
Size: 729 248 2910
Fragments arranged by size:
2910 729 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
SmaI CCC'GGG
Cuts at: 156 595 909 156
Size: 439 314 3134
Fragments arranged by size:
3134 439 314
SmlI C'TyrA_G
Cuts at: 1042 1957 2449 2711 1042
Size: 915 492 262 2218
Fragments arranged by size:
2218 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3887
SphI G_CATG'C
Cuts at: 940 1440 940
Size: 500 3387
SrfI GCCC'GGGC
Cuts at: 156 156
Size: 3887
StyI C'CwwG_G
Cuts at: 1021 1375 1021
Size: 354 3533
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 697 770 1470 1603 2062 2271 697
Size: 73 700 133 459 209 2313
Fragments arranged by size:
2313 700 459 209 133 73
TatI w'GTAC_w
Cuts at: 314 3402 314
Size: 3088 799
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 546 578 3404 3436 546
Size: 32 2826 32 997
Fragments arranged by size:
2826 997 32 32
Tth111I GACn'n_nGTC
Cuts at: 1684 1684
Size: 3887
XbaI T'CTAG_A
Cuts at: 918 918
Size: 3887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 285 1382 285
Size: 1097 2790
XmaI C'CCGG_G
Cuts at: 154 593 907 154
Size: 439 314 3134
Fragments arranged by size:
3134 439 314
XmnI GAAnn'nnTTC
Cuts at: 532 532
Size: 3887
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI Bpu10I BsiWI BsmI
BsmBI BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SspI StuI
SwaI XhoI ZraI