Restriction map for
pF1KB5289
(Circular) (Six-base) MAPSORT of: pF1KB5289.seq Check: 291 from: 1 to: 4367
pF1KB5289 4367 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 1405 1405
Size: 4367
Acc65I G'GTAC_C
Cuts at: 466 1383 466
Size: 917 3450
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1042 1078 2192 2624 3371 3639 1042
Size: 36 1114 432 747 268 1770
Fragments arranged by size:
1770 1114 747 432 268 36
AflIII A'CryG_T
Cuts at: 1719 2823 3800 1719
Size: 1104 977 2286
Fragments arranged by size:
2286 1104 977
AgeI A'CCGG_T
Cuts at: 2755 2755
Size: 4367
AlwNI CAG_nnn'CTG
Cuts at: 117 161 277 1672 3239 117
Size: 44 116 1395 1567 1245
Fragments arranged by size:
1567 1395 1245 116 44
ApaI G_GGCC'C
Cuts at: 1323 1360 1323
Size: 37 4330
ApaLI G'TGCA_C
Cuts at: 3137 3137
Size: 4367
ApoI r'AATT_y
Cuts at: 701 960 1371 3720 3976 701
Size: 259 411 2349 256 1092
Fragments arranged by size:
2349 1092 411 259 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 148 790 1387 148
Size: 642 597 3128
Fragments arranged by size:
3128 642 597
BamHI G'GATC_C
Cuts at: 237 1392 237
Size: 1155 3212
BanI G'GyrC_C
Cuts at: 466 573 1383 1797 2045 2080 466
Size: 107 810 414 248 35 2753
Fragments arranged by size:
2753 810 414 248 107 35
BanII G_rGCy'C
Cuts at: 144 196 328 429 482 1323 1360 1381 2411
Size: 52 132 101 53 841 37 21 1030
Cuts at: 2411 144
Size: 2100
Fragments arranged by size:
2100 1030 841 132 101 53 52 37
21
BbeI G_GCGC'C
Cuts at: 1801 2049 1801
Size: 248 4119
BbsI GAAGACnn'nnnn_
Cuts at: 1030 1030
Size: 4367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 321 355 90
Size: 34 197 34 4102
Fragments arranged by size:
4102 197 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3026 4367 2265
Size: 761 1341 2265
Fragments arranged by size:
2265 1341 761
BclI T'GATC_A
Cuts at: 443 1887 443
Size: 1444 2923
BglI GCCn_nnn'nGGC
Cuts at: 112 376 112
Size: 264 4103
BglII A'GATC_T
Cuts at: 879 1882 3919 879
Size: 1003 2037 1327
Fragments arranged by size:
2037 1327 1003
BlpI GC'TnA_GC
Cuts at: 1479 1479
Size: 4367
Bme1580I G_kGCm'C
Cuts at: 155 1323 1360 1992 2085 3141 155
Size: 1168 37 632 93 1056 1381
Fragments arranged by size:
1381 1168 1056 632 93 37
BmrI ACTGGGnnnn_n'
Cuts at: 663 1579 1660 1995 4139 663
Size: 916 81 335 2144 891
Fragments arranged by size:
2144 916 891 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 163 210 2734 163
Size: 47 2524 1796
Fragments arranged by size:
2524 1796 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 759 759
Size: 4367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 1543 2422 2914 3212 499
Size: 1044 879 492 298 1654
Fragments arranged by size:
1654 1044 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4071 4071
Size: 4367
BsaAI yAC'GTr
Cuts at: 1720 1720
Size: 4367
BsaBI GATnn'nnATC
Cuts at: 6 878 1881 3918 6
Size: 872 1003 2037 455
Fragments arranged by size:
2037 1003 872 455
BsaHI Gr'CG_yC
Cuts at: 1798 2046 2748 4244 1798
Size: 248 702 1496 1921
Fragments arranged by size:
1921 1496 702 248
BsaWI w'CCGG_w
Cuts at: 913 1556 2077 2755 3029 3176 3587 913
Size: 643 521 678 274 147 411 1693
Fragments arranged by size:
1693 678 643 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 118 136 202 244 247 341 991 118
Size: 18 66 42 3 94 650 3494
Fragments arranged by size:
3494 650 94 66 42 18 3
BseYI C'CCAG_C
Cuts at: 387 899 1225 1792 2550 3127 3623 387
Size: 512 326 567 758 577 496 1131
Fragments arranged by size:
1131 758 577 567 512 496 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 1051 679
Size: 372 3995
BsiEI CG_ry'CG
Cuts at: 104 1909 1955 3163 104
Size: 1805 46 1208 1308
Fragments arranged by size:
1805 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 344 482 1381 2159 2349 3141 344
Size: 138 899 778 190 792 1570
Fragments arranged by size:
1570 899 792 778 190 138
BsmI GAATG_Cn'
Cuts at: 719 719
Size: 4367
Bsp1286I G_dGCh'C
Cuts at: 144 155 196 328 344 429 482 1323 1360
Size: 11 41 132 16 85 53 841 37
Cuts at: 1360 1381 1992 2085 2159 2349 2411 3141 144
Size: 21 611 93 74 190 62 730 1370
Fragments arranged by size:
1370 841 730 611 190 132 93 85
74 62 53 41 37 21 16 11
BspEI T'CCGG_A
Cuts at: 3587 3587
Size: 4367
BspHI T'CATG_A
Cuts at: 1002 3543 1002
Size: 2541 1826
BspMI ACCTGCnnnn'nnnn_
Cuts at: 644 1417 1631 1933 2314 644
Size: 773 214 302 381 2697
Fragments arranged by size:
2697 773 381 302 214
BsrBI CCG'CTC
Cuts at: 50 394 2659 2713 4197 4362 50
Size: 344 2265 54 1484 165 55
Fragments arranged by size:
2265 1484 344 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2279 2279
Size: 4367
BsrFI r'CCGG_y
Cuts at: 2365 2546 2755 2365
Size: 181 209 3977
Fragments arranged by size:
3977 209 181
BssSI C'ACGA_G
Cuts at: 2638 2996 2638
Size: 358 4009
BstAPI GCAn_nnn'nTGC
Cuts at: 1744 1744
Size: 4367
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstXI CCAn_nnnn'nTGG
Cuts at: 570 570
Size: 4367
BstYI r'GATC_y
Cuts at: 1 237 879 1392 1859 1882 2217 2463 3475
Size: 236 642 513 467 23 335 246 1012
Cuts at: 3475 3561 3573 3919 1
Size: 86 12 346 449
Fragments arranged by size:
1012 642 513 467 449 346 335 246
236 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 848 848
Size: 4367
BtgI C'CryG_G
Cuts at: 365 462 365
Size: 97 4270
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2317 2498 2661 2317
Size: 181 163 4023
Fragments arranged by size:
4023 181 163
BtsI GCAGTG_nn'
Cuts at: 494 760 1610 494
Size: 266 850 3251
Fragments arranged by size:
3251 850 266
DraI TTT'AAA
Cuts at: 1366 1366
Size: 4367
DrdI GACnn_nn'nnGTC
Cuts at: 2073 2931 2073
Size: 858 3509
EaeI y'GGCC_r
Cuts at: 362 1952 2126 2517 2544 3889 362
Size: 1590 174 391 27 1345 840
Fragments arranged by size:
1590 1345 840 391 174 27
EagI C'GGCC_G
Cuts at: 1952 1952
Size: 4367
EarI CTCTTCn'nnn_
Cuts at: 806 1333 2390 2600 806
Size: 527 1057 210 2573
Fragments arranged by size:
2573 1057 527 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2575 2885 3031 4177 2575
Size: 310 146 1146 2765
Fragments arranged by size:
2765 1146 310 146
EcoICRI GAG'CTC
Cuts at: 480 1379 480
Size: 899 3468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 759 1042 1078 2192 2624 3371 3639 759
Size: 283 36 1114 432 747 268 1487
Fragments arranged by size:
1487 1114 747 432 283 268 36
EcoO109I rG'GnC_Cy
Cuts at: 114 828 1319 1320 1356 1357 1506 114
Size: 714 491 1 36 1 149 2975
Fragments arranged by size:
2975 714 491 149 36 1 1
EcoRI G'AATT_C
Cuts at: 1371 1371
Size: 4367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4367
FspI TGC'GCA
Cuts at: 553 2148 553
Size: 1595 2772
HaeII r_GCGC'y
Cuts at: 223 1801 2049 3071 4050 223
Size: 1578 248 1022 979 540
Fragments arranged by size:
1578 1022 979 540 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2209 2239 2241 2271 2209
Size: 30 2 30 4305
Fragments arranged by size:
4305 30 30 2
HincII GTy'rAC
Cuts at: 1406 1406
Size: 4367
KasI G'GCGC_C
Cuts at: 1797 2045 1797
Size: 248 4119
KpnI G_GTAC'C
Cuts at: 470 1387 470
Size: 917 3450
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 308 821 3038 3222 308
Size: 513 2217 184 1453
Fragments arranged by size:
2217 1453 513 184
MscI TGG'CCA
Cuts at: 364 2128 364
Size: 1764 2603
MslI CAynn'nnrTG
Cuts at: 2356 2483 2356
Size: 127 4240
MspA1I CmG'CkG
Cuts at: 161 274 887 903 1263 1478 1792 2152 3165
Size: 113 613 16 360 215 314 360 1013
Cuts at: 3165 3410 161
Size: 245 1118
Fragments arranged by size:
1118 1013 613 360 360 314 245 215
113 16
NaeI GCC'GGC
Cuts at: 2548 2548
Size: 4367
NarI GG'CG_CC
Cuts at: 1798 2046 1798
Size: 248 4119
NcoI C'CATG_G
Cuts at: 462 462
Size: 4367
NdeI CA'TA_TG
Cuts at: 591 591
Size: 4367
NgoMIV G'CCGG_C
Cuts at: 2546 2546
Size: 4367
NspI r_CATG'y
Cuts at: 1420 1920 2827 1420
Size: 500 907 2960
Fragments arranged by size:
2960 907 500
PasI CC'CwG_GG
Cuts at: 1231 1352 1231
Size: 121 4246
PciI A'CATG_T
Cuts at: 2823 2823
Size: 4367
PfoI T'CCnGG_A
Cuts at: 526 526
Size: 4367
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 4335
PpuMI rG'GwC_Cy
Cuts at: 828 828
Size: 4367
PspOMI G'GGCC_C
Cuts at: 1319 1356 1319
Size: 37 4330
PspXI vC'TCGA_Gb
Cuts at: 790 790
Size: 4367
PstI C_TGCA'G
Cuts at: 1097 1241 1414 2099 1097
Size: 144 173 685 3365
Fragments arranged by size:
3365 685 173 144
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 161 887 903 1792 2152 161
Size: 726 16 889 360 2376
Fragments arranged by size:
2376 889 726 360 16
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 482 1381 482
Size: 899 3468
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SanDI GG'GwC_CC
Cuts at: 828 828
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 2390 2600 2390
Size: 210 4157
SbfI CC_TGCA'GG
Cuts at: 1097 1414 1097
Size: 317 4050
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SfcI C'TryA_G
Cuts at: 33 1093 1237 1410 2095 3088 3279 33
Size: 1060 144 173 685 993 191 1121
Fragments arranged by size:
1121 1060 993 685 191 173 144
SfiI GGCCn_nnn'nGGCC
Cuts at: 376 376
Size: 4367
SfoI GGC'GCC
Cuts at: 1799 2047 1799
Size: 248 4119
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 1389 1389
Size: 4367
SmlI C'TyrA_G
Cuts at: 514 790 1522 2437 2929 3191 514
Size: 276 732 915 492 262 1690
Fragments arranged by size:
1690 915 732 492 276 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 1420 1920 1420
Size: 500 3867
StyI C'CwwG_G
Cuts at: 462 1501 1855 462
Size: 1039 354 2974
Fragments arranged by size:
2974 1039 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 645 1028 1950 2083 2542 2751 645
Size: 383 922 133 459 209 2261
Fragments arranged by size:
2261 922 459 383 209 133
TatI w'GTAC_w
Cuts at: 3882 3882
Size: 4367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3884 3916 3884
Size: 32 4335
Tth111I GACn'n_nGTC
Cuts at: 490 2164 490
Size: 1674 2693
XbaI T'CTAG_A
Cuts at: 1398 1398
Size: 4367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1862 114
Size: 1748 2619
XhoI C'TCGA_G
Cuts at: 790 790
Size: 4367
XmaI C'CCGG_G
Cuts at: 1387 1387
Size: 4367
XmnI GAAnn'nnTTC
Cuts at: 783 783
Size: 4367
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PflMI PmlI
PshAI PsiI PsrI SacII SexAI SgrAI SnaBI SrfI
SspI StuI SwaI ZraI