Restriction map for
pF1KB5209
(Circular) (Six-base) MAPSORT of: pF1KB5209.seq Check: 6492 from: 1 to: 4694
pF1KB5209 4694 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 1732 1732
Size: 4694
Acc65I G'GTAC_C
Cuts at: 1710 1710
Size: 4694
AclI AA'CG_TT
Cuts at: 1688 4534 1688
Size: 2846 1848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 2519 2951 3304 3966 358
Size: 2161 432 353 662 1086
Fragments arranged by size:
2161 1086 662 432 353
AflIII A'CryG_T
Cuts at: 410 780 1202 2046 3846 4127 410
Size: 370 422 844 1800 281 977
Fragments arranged by size:
1800 977 844 422 370 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 219 1285 1309 1459 1665 219
Size: 1066 24 150 206 3248
Fragments arranged by size:
3248 1066 206 150 24
AlwNI CAG_nnn'CTG
Cuts at: 960 1999 3437 960
Size: 1039 1438 2217
Fragments arranged by size:
2217 1438 1039
ApaI G_GGCC'C
Cuts at: 811 1612 811
Size: 801 3893
ApaLI G'TGCA_C
Cuts at: 3532 3532
Size: 4694
ApoI r'AATT_y
Cuts at: 1072 1698 4047 4303 1072
Size: 626 2349 256 1463
Fragments arranged by size:
2349 1463 626 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4694
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4694
AvaI C'yCGr_G
Cuts at: 1714 1714
Size: 4694
BamHI G'GATC_C
Cuts at: 1527 1719 1527
Size: 192 4502
BanI G'GyrC_C
Cuts at: 203 1622 1710 2124 2372 2407 203
Size: 1419 88 414 248 35 2490
Fragments arranged by size:
2490 1419 414 248 88 35
BanII G_rGCy'C
Cuts at: 193 811 1019 1496 1612 1708 2738 193
Size: 618 208 477 116 96 1030 2149
Fragments arranged by size:
2149 1030 618 477 208 116 96
BbeI G_GCGC'C
Cuts at: 2128 2376 2128
Size: 248 4446
BbsI GAAGACnn'nnnn_
Cuts at: 223 388 223
Size: 165 4529
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1641 1675 1641
Size: 34 4660
BciVI GTATCCnnnnn_n'
Cuts at: 2592 3648 4694 2592
Size: 1056 1046 2592
Fragments arranged by size:
2592 1056 1046
BclI T'GATC_A
Cuts at: 1470 2214 1470
Size: 744 3950
BglI GCCn_nnn'nGGC
Cuts at: 140 976 140
Size: 836 3858
BglII A'GATC_T
Cuts at: 905 2209 4246 905
Size: 1304 2037 1353
Fragments arranged by size:
2037 1353 1304
BlpI GC'TnA_GC
Cuts at: 1806 1806
Size: 4694
Bme1580I G_kGCm'C
Cuts at: 811 1612 2319 2412 3536 811
Size: 801 707 93 1124 1969
Fragments arranged by size:
1969 1124 801 707 93
BmrI ACTGGGnnnn_n'
Cuts at: 1641 1906 1987 2322 4466 1641
Size: 265 81 335 2144 1869
Fragments arranged by size:
2144 1869 335 265 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 519 3061 519
Size: 2542 2152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 589 1255 1405 514
Size: 75 666 150 3803
Fragments arranged by size:
3803 666 150 75
Bpu10I CC'TnA_GC
Cuts at: 1250 1250
Size: 4694
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1444 1870 2749 3463 3761 1444
Size: 426 879 714 298 2377
Fragments arranged by size:
2377 879 714 426 298
BsaI GGTCTCn'nnnn_
Cuts at: 4398 4398
Size: 4694
BsaAI yAC'GTr
Cuts at: 1205 1314 2047 1205
Size: 109 733 3852
Fragments arranged by size:
3852 733 109
BsaBI GATnn'nnATC
Cuts at: 6 1507 2208 4245 6
Size: 1501 701 2037 455
Fragments arranged by size:
2037 1501 701 455
BsaHI Gr'CG_yC
Cuts at: 223 2125 2373 3075 4571 223
Size: 1902 248 702 1496 346
Fragments arranged by size:
1902 1496 702 346 248
BsaWI w'CCGG_w
Cuts at: 1883 2404 3082 3493 3640 3914 1883
Size: 521 678 411 147 274 2663
Fragments arranged by size:
2663 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1198 1228 1198
Size: 30 4664
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 370 373 628 631 805 1252 370
Size: 3 255 3 174 447 3812
Fragments arranged by size:
3812 447 255 174 3 3
BseYI C'CCAG_C
Cuts at: 674 1038 2119 2877 3542 3950 674
Size: 364 1081 758 665 408 1418
Fragments arranged by size:
1418 1081 758 665 408 364
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1255 1281 1283 1255
Size: 26 2 4666
Fragments arranged by size:
4666 26 2
BsiEI CG_ry'CG
Cuts at: 104 2236 2282 3512 104
Size: 2132 46 1230 1286
Fragments arranged by size:
2132 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 505 1156 1708 2486 2676 3536 505
Size: 651 552 778 190 860 1663
Fragments arranged by size:
1663 860 778 651 552 190
BsiWI C'GTAC_G
Cuts at: 1205 1205
Size: 4694
BsmI GAATG_Cn'
Cuts at: 184 710 184
Size: 526 4168
Bsp1286I G_dGCh'C
Cuts at: 193 505 811 1019 1156 1496 1612 1708 2319
Size: 312 306 208 137 340 116 96 611
Cuts at: 2319 2412 2486 2676 2738 3536 193
Size: 93 74 190 62 798 1351
Fragments arranged by size:
1351 798 611 340 312 306 208 190
137 116 96 93 74 62
BspHI T'CATG_A
Cuts at: 3126 3126
Size: 4694
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1744 1958 2260 2641 1744
Size: 214 302 381 3797
Fragments arranged by size:
3797 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2986 3040 4524 4689 50
Size: 2936 54 1484 165 55
Fragments arranged by size:
2936 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1659 1665 2606 1659
Size: 6 941 3747
Fragments arranged by size:
3747 941 6
BsrFI r'CCGG_y
Cuts at: 154 2692 2873 154
Size: 2538 181 1975
Fragments arranged by size:
2538 1975 181
BssSI C'ACGA_G
Cuts at: 1030 2965 3673 1030
Size: 1935 708 2051
Fragments arranged by size:
2051 1935 708
BstAPI GCAn_nnn'nTGC
Cuts at: 252 2071 252
Size: 1819 2875
BstBI TT'CG_AA
Cuts at: 3055 3055
Size: 4694
BstEII G'GTnAC_C
Cuts at: 1089 1089
Size: 4694
BstXI CCAn_nnnn'nTGG
Cuts at: 138 1233 1619 138
Size: 1095 386 3213
Fragments arranged by size:
3213 1095 386
BstYI r'GATC_y
Cuts at: 1 362 581 905 1409 1508 1527 1719 2186
Size: 361 219 324 504 99 19 192 467
Cuts at: 2186 2209 2544 2790 3096 3108 3194 4246 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 504 467 449 361 335 324 306
246 219 192 99 86 23 19 12
Bsu36I CC'TnA_GG
Cuts at: 650 650
Size: 4694
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2644 2825 2988 2644
Size: 181 163 4350
Fragments arranged by size:
4350 181 163
BtsI GCAGTG_nn'
Cuts at: 203 245 246 1471 1937 203
Size: 42 1 1225 466 2960
Fragments arranged by size:
2960 1225 466 42 1
DraI TTT'AAA
Cuts at: 1693 1693
Size: 4694
DrdI GACnn_nn'nnGTC
Cuts at: 2400 3744 2400
Size: 1344 3350
EaeI y'GGCC_r
Cuts at: 128 623 818 2279 2453 2844 2871 4216 128
Size: 495 195 1461 174 391 27 1345 606
Fragments arranged by size:
1461 1345 606 495 391 195 174 27
EagI C'GGCC_G
Cuts at: 2279 2279
Size: 4694
EarI CTCTTCn'nnn_
Cuts at: 124 1175 2717 2927 124
Size: 1051 1542 210 1891
Fragments arranged by size:
1891 1542 1051 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2902 3644 3790 4504 2902
Size: 742 146 714 3092
Fragments arranged by size:
3092 742 714 146
EcoICRI GAG'CTC
Cuts at: 1706 1706
Size: 4694
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 514 589 1255 1405 2519 2951 3304 3966
Size: 156 75 666 150 1114 432 353 662
Cuts at: 3966 358
Size: 1086
Fragments arranged by size:
1114 1086 666 662 432 353 156 150
75
EcoNI CCTnn'n_nnAGG
Cuts at: 1081 1234 1516 1081
Size: 153 282 4259
Fragments arranged by size:
4259 282 153
EcoO109I rG'GnC_Cy
Cuts at: 808 1035 1608 1833 808
Size: 227 573 225 3669
Fragments arranged by size:
3669 573 227 225
EcoRI G'AATT_C
Cuts at: 1072 1698 1072
Size: 626 4068
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 4694
FspI TGC'GCA
Cuts at: 2475 2475
Size: 4694
HaeII r_GCGC'y
Cuts at: 2128 2376 3606 4377 2128
Size: 248 1230 771 2445
Fragments arranged by size:
2445 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2536 2566 2568 2598 2536
Size: 30 2 30 4632
Fragments arranged by size:
4632 30 30 2
HincII GTy'rAC
Cuts at: 1733 1733
Size: 4694
KasI G'GCGC_C
Cuts at: 2124 2372 2124
Size: 248 4446
KpnI G_GTAC'C
Cuts at: 1714 1714
Size: 4694
MluI A'CGCG_T
Cuts at: 4127 4127
Size: 4694
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 251 1157 3453 3637 251
Size: 906 2296 184 1308
Fragments arranged by size:
2296 1308 906 184
MscI TGG'CCA
Cuts at: 130 625 2455 130
Size: 495 1830 2369
Fragments arranged by size:
2369 1830 495
MslI CAynn'nnrTG
Cuts at: 715 2683 2810 715
Size: 1968 127 2599
Fragments arranged by size:
2599 1968 127
MspA1I CmG'CkG
Cuts at: 325 567 1805 2119 2479 3263 3508 325
Size: 242 1238 314 360 784 245 1511
Fragments arranged by size:
1511 1238 784 360 314 245 242
NaeI GCC'GGC
Cuts at: 156 2875 156
Size: 2719 1975
NarI GG'CG_CC
Cuts at: 2125 2373 2125
Size: 248 4446
NdeI CA'TA_TG
Cuts at: 301 826 301
Size: 525 4169
NgoMIV G'CCGG_C
Cuts at: 154 2873 154
Size: 2719 1975
NspI r_CATG'y
Cuts at: 414 784 885 1269 1747 2247 3850 414
Size: 370 101 384 478 500 1603 1258
Fragments arranged by size:
1603 1258 500 478 384 370 101
PciI A'CATG_T
Cuts at: 410 780 3846 410
Size: 370 3066 1258
Fragments arranged by size:
3066 1258 370
PflMI CCAn_nnn'nTGG
Cuts at: 771 1194 771
Size: 423 4271
PfoI T'CCnGG_A
Cuts at: 458 458
Size: 4694
PmeI GTTT'AAAC
Cuts at: 1693 1693
Size: 4694
PmlI CAC'GTG
Cuts at: 1314 1314
Size: 4694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3140 3172 3140
Size: 32 4662
PpuMI rG'GwC_Cy
Cuts at: 1035 1035
Size: 4694
PshAI GACnn'nnGTC
Cuts at: 961 961
Size: 4694
PspOMI G'GGCC_C
Cuts at: 807 1608 807
Size: 801 3893
PstI C_TGCA'G
Cuts at: 257 1438 1741 2426 257
Size: 1181 303 685 2525
Fragments arranged by size:
2525 1181 685 303
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4694
PvuII CAG'CTG
Cuts at: 325 567 2119 2479 325
Size: 242 1552 360 2540
Fragments arranged by size:
2540 1552 360 242
RsrII CG'GwC_CG
Cuts at: 2889 2889
Size: 4694
SacI G_AGCT'C
Cuts at: 1708 1708
Size: 4694
SalI G'TCGA_C
Cuts at: 1731 1731
Size: 4694
SapI GCTCTTCn'nnn_
Cuts at: 2717 2927 2717
Size: 210 4484
SbfI CC_TGCA'GG
Cuts at: 1741 1741
Size: 4694
ScaI AGT'ACT
Cuts at: 4211 4211
Size: 4694
SfcI C'TryA_G
Cuts at: 33 253 1434 1737 2422 3390 3581 33
Size: 220 1181 303 685 968 191 1146
Fragments arranged by size:
1181 1146 968 685 303 220 191
SfoI GGC'GCC
Cuts at: 2126 2374 2126
Size: 248 4446
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4694
SmaI CCC'GGG
Cuts at: 1716 1716
Size: 4694
SmlI C'TyrA_G
Cuts at: 1423 1849 2764 3478 3740 1423
Size: 426 915 714 262 2377
Fragments arranged by size:
2377 915 714 426 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4694
SphI G_CATG'C
Cuts at: 1269 1747 2247 1269
Size: 478 500 3716
Fragments arranged by size:
3716 500 478
StuI AGG'CCT
Cuts at: 769 769
Size: 4694
StyI C'CwwG_G
Cuts at: 165 770 899 1828 2182 165
Size: 605 129 929 354 2677
Fragments arranged by size:
2677 929 605 354 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2277 2410 2869 3078 2277
Size: 133 459 209 3893
Fragments arranged by size:
3893 459 209 133
TatI w'GTAC_w
Cuts at: 4209 4209
Size: 4694
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4211 4243 4211
Size: 32 4662
Tth111I GACn'n_nGTC
Cuts at: 222 313 2491 222
Size: 91 2178 2425
Fragments arranged by size:
2425 2178 91
XbaI T'CTAG_A
Cuts at: 1725 1725
Size: 4694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 957 2189 957
Size: 1232 3462
XmaI C'CCGG_G
Cuts at: 1714 1714
Size: 4694
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI BsmBI
BspEI BsrGI BssHII BstZ17I BtgI ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NheI
NotI NruI NsiI PacI PasI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI