Restriction map for
pF1KB5204
(Circular) (Six-base) MAPSORT of: pF1KB5204.seq Check: 9143 from: 1 to: 3920
pF1KB5204 3920 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 958 958
Size: 3920
Acc65I G'GTAC_C
Cuts at: 936 936
Size: 3920
AclI AA'CG_TT
Cuts at: 3760 3760
Size: 3920
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1745 2177 2924 3192 1745
Size: 432 747 268 2473
Fragments arranged by size:
2473 747 432 268
AflIII A'CryG_T
Cuts at: 1272 2376 3353 1272
Size: 1104 977 1839
Fragments arranged by size:
1839 1104 977
AgeI A'CCGG_T
Cuts at: 2308 2308
Size: 3920
AhdI GACnn_n'nnGTC
Cuts at: 459 459
Size: 3920
AleI CACnn'nnGTG
Cuts at: 287 287
Size: 3920
AlwNI CAG_nnn'CTG
Cuts at: 174 189 1225 2792 174
Size: 15 1036 1567 1302
Fragments arranged by size:
1567 1302 1036 15
ApaI G_GGCC'C
Cuts at: 587 587
Size: 3920
ApaLI G'TGCA_C
Cuts at: 2690 2690
Size: 3920
ApoI r'AATT_y
Cuts at: 232 595 924 3273 3529 232
Size: 363 329 2349 256 623
Fragments arranged by size:
2349 623 363 329 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3920
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
AvaI C'yCGr_G
Cuts at: 388 457 940 388
Size: 69 483 3368
Fragments arranged by size:
3368 483 69
BamHI G'GATC_C
Cuts at: 945 945
Size: 3920
BanI G'GyrC_C
Cuts at: 524 936 1350 1598 1633 524
Size: 412 414 248 35 2811
Fragments arranged by size:
2811 414 412 248 35
BanII G_rGCy'C
Cuts at: 587 934 1964 587
Size: 347 1030 2543
Fragments arranged by size:
2543 1030 347
BbeI G_GCGC'C
Cuts at: 1354 1602 1354
Size: 248 3672
BbsI GAAGACnn'nnnn_
Cuts at: 304 304
Size: 3920
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3886
BciVI GTATCCnnnnn_n'
Cuts at: 1818 2579 3920 1818
Size: 761 1341 1818
Fragments arranged by size:
1818 1341 761
BclI T'GATC_A
Cuts at: 1440 1440
Size: 3920
BglII A'GATC_T
Cuts at: 1435 3472 1435
Size: 2037 1883
BlpI GC'TnA_GC
Cuts at: 1032 1032
Size: 3920
Bme1580I G_kGCm'C
Cuts at: 587 893 1545 1638 2694 587
Size: 306 652 93 1056 1813
Fragments arranged by size:
1813 1056 652 306 93
BmrI ACTGGGnnnn_n'
Cuts at: 1132 1213 1548 3692 1132
Size: 81 335 2144 1360
Fragments arranged by size:
2144 1360 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 603 2287 603
Size: 1684 2236
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 847 653
Size: 194 3726
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1975 2467 2765 1096
Size: 879 492 298 2251
Fragments arranged by size:
2251 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3624 3624
Size: 3920
BsaAI yAC'GTr
Cuts at: 1273 1273
Size: 3920
BsaBI GATnn'nnATC
Cuts at: 6 1434 3471 6
Size: 1428 2037 455
Fragments arranged by size:
2037 1428 455
BsaHI Gr'CG_yC
Cuts at: 1351 1599 2301 3797 1351
Size: 248 702 1496 1474
Fragments arranged by size:
1496 1474 702 248
BsaWI w'CCGG_w
Cuts at: 1109 1630 2308 2582 2729 3140 1109
Size: 521 678 274 147 411 1889
Fragments arranged by size:
1889 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 388 418 651 681 388
Size: 30 233 30 3627
Fragments arranged by size:
3627 233 30 30
BseYI C'CCAG_C
Cuts at: 174 1345 2103 2680 3176 174
Size: 1171 758 577 496 918
Fragments arranged by size:
1171 918 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 441 430
Size: 11 3909
BsiEI CG_ry'CG
Cuts at: 104 1462 1508 2716 104
Size: 1358 46 1208 1308
Fragments arranged by size:
1358 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 934 1712 1902 2694 934
Size: 778 190 792 2160
Fragments arranged by size:
2160 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 587 893 934 1545 1638 1712 1902 1964 2694
Size: 306 41 611 93 74 190 62 730
Cuts at: 2694 587
Size: 1813
Fragments arranged by size:
1813 730 611 306 190 93 74 62
41
BspEI T'CCGG_A
Cuts at: 3140 3140
Size: 3920
BspHI T'CATG_A
Cuts at: 3096 3096
Size: 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 756 822 970 1184 1486 1867 756
Size: 66 148 214 302 381 2809
Fragments arranged by size:
2809 381 302 214 148 66
BsrBI CCG'CTC
Cuts at: 50 2212 2266 3750 3915 50
Size: 2162 54 1484 165 55
Fragments arranged by size:
2162 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1832 1832
Size: 3920
BsrFI r'CCGG_y
Cuts at: 527 1918 2099 2308 527
Size: 1391 181 209 2139
Fragments arranged by size:
2139 1391 209 181
BssSI C'ACGA_G
Cuts at: 562 588 2191 2549 562
Size: 26 1603 358 1933
Fragments arranged by size:
1933 1603 358 26
BstAPI GCAn_nnn'nTGC
Cuts at: 189 1297 189
Size: 1108 2812
BstBI TT'CG_AA
Cuts at: 2281 2281
Size: 3920
BstEII G'GTnAC_C
Cuts at: 834 834
Size: 3920
BstXI CCAn_nnnn'nTGG
Cuts at: 289 289
Size: 3920
BstYI r'GATC_y
Cuts at: 1 945 1412 1435 1770 2016 3028 3114 3126
Size: 944 467 23 335 246 1012 86 12
Cuts at: 3126 3472 1
Size: 346 449
Fragments arranged by size:
1012 944 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 492 623 492
Size: 131 3789
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 105 1870 2051 2214 105
Size: 1765 181 163 1811
Fragments arranged by size:
1811 1765 181 163
BtsI GCAGTG_nn'
Cuts at: 646 1163 646
Size: 517 3403
DraI TTT'AAA
Cuts at: 919 919
Size: 3920
DrdI GACnn_nn'nnGTC
Cuts at: 1626 2484 1626
Size: 858 3062
EaeI y'GGCC_r
Cuts at: 820 1505 1679 2070 2097 3442 820
Size: 685 174 391 27 1345 1298
Fragments arranged by size:
1345 1298 685 391 174 27
EagI C'GGCC_G
Cuts at: 1505 1505
Size: 3920
EarI CTCTTCn'nnn_
Cuts at: 744 1943 2153 744
Size: 1199 210 2511
Fragments arranged by size:
2511 1199 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 680 2128 2438 2584 3730 680
Size: 1448 310 146 1146 870
Fragments arranged by size:
1448 1146 870 310 146
EcoICRI GAG'CTC
Cuts at: 932 932
Size: 3920
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 847 1745 2177 2924 3192 653
Size: 194 898 432 747 268 1381
Fragments arranged by size:
1381 898 747 432 268 194
EcoO109I rG'GnC_Cy
Cuts at: 583 584 814 1059 583
Size: 1 230 245 3444
Fragments arranged by size:
3444 245 230 1
EcoRI G'AATT_C
Cuts at: 232 924 232
Size: 692 3228
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 165 1939 165
Size: 1774 2146
FspI TGC'GCA
Cuts at: 1701 1701
Size: 3920
HaeII r_GCGC'y
Cuts at: 221 1354 1602 2624 3603 221
Size: 1133 248 1022 979 538
Fragments arranged by size:
1133 1022 979 538 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 445 477 651 683 1762 1792 1794 1824 445
Size: 32 174 32 1079 30 2 30 2541
Fragments arranged by size:
2541 1079 174 32 32 30 30 2
HincII GTy'rAC
Cuts at: 725 959 725
Size: 234 3686
HpaI GTT'AAC
Cuts at: 725 725
Size: 3920
KasI G'GCGC_C
Cuts at: 1350 1598 1350
Size: 248 3672
KpnI G_GTAC'C
Cuts at: 940 940
Size: 3920
MluI A'CGCG_T
Cuts at: 3353 3353
Size: 3920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2591 2775 2591
Size: 184 3736
MscI TGG'CCA
Cuts at: 1681 1681
Size: 3920
MslI CAynn'nnrTG
Cuts at: 287 368 550 1909 2036 287
Size: 81 182 1359 127 2171
Fragments arranged by size:
2171 1359 182 127 81
MspA1I CmG'CkG
Cuts at: 174 189 1031 1345 1705 2718 2963 174
Size: 15 842 314 360 1013 245 1131
Fragments arranged by size:
1131 1013 842 360 314 245 15
NaeI GCC'GGC
Cuts at: 2101 2101
Size: 3920
NarI GG'CG_CC
Cuts at: 1351 1599 1351
Size: 248 3672
NcoI C'CATG_G
Cuts at: 623 623
Size: 3920
NgoMIV G'CCGG_C
Cuts at: 2099 2099
Size: 3920
NspI r_CATG'y
Cuts at: 270 973 1473 2380 270
Size: 703 500 907 1810
Fragments arranged by size:
1810 907 703 500
PciI A'CATG_T
Cuts at: 2376 2376
Size: 3920
PmeI GTTT'AAAC
Cuts at: 919 919
Size: 3920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 3888
PpuMI rG'GwC_Cy
Cuts at: 814 814
Size: 3920
PspOMI G'GGCC_C
Cuts at: 583 583
Size: 3920
PstI C_TGCA'G
Cuts at: 523 967 1652 523
Size: 444 685 2791
Fragments arranged by size:
2791 685 444
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3920
PvuII CAG'CTG
Cuts at: 174 1345 1705 174
Size: 1171 360 2389
Fragments arranged by size:
2389 1171 360
RsrII CG'GwC_CG
Cuts at: 2115 2115
Size: 3920
SacI G_AGCT'C
Cuts at: 934 934
Size: 3920
SalI G'TCGA_C
Cuts at: 957 957
Size: 3920
SanDI GG'GwC_CC
Cuts at: 814 814
Size: 3920
SapI GCTCTTCn'nnn_
Cuts at: 1943 2153 1943
Size: 210 3710
SbfI CC_TGCA'GG
Cuts at: 967 967
Size: 3920
ScaI AGT'ACT
Cuts at: 3437 3437
Size: 3920
SfcI C'TryA_G
Cuts at: 33 519 963 1648 2641 2832 33
Size: 486 444 685 993 191 1121
Fragments arranged by size:
1121 993 685 486 444 191
SfoI GGC'GCC
Cuts at: 1352 1600 1352
Size: 248 3672
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
SmaI CCC'GGG
Cuts at: 942 942
Size: 3920
SmlI C'TyrA_G
Cuts at: 1075 1990 2482 2744 1075
Size: 915 492 262 2251
Fragments arranged by size:
2251 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3920
SphI G_CATG'C
Cuts at: 973 1473 973
Size: 500 3420
StyI C'CwwG_G
Cuts at: 481 623 1054 1408 481
Size: 142 431 354 2993
Fragments arranged by size:
2993 431 354 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1503 1636 2095 2304 1503
Size: 133 459 209 3119
Fragments arranged by size:
3119 459 209 133
TatI w'GTAC_w
Cuts at: 3435 3435
Size: 3920
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3437 3469 3437
Size: 32 3888
Tth111I GACn'n_nGTC
Cuts at: 1717 1717
Size: 3920
XbaI T'CTAG_A
Cuts at: 951 951
Size: 3920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 891 1415 891
Size: 524 3396
XmaI C'CCGG_G
Cuts at: 940 940
Size: 3920
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI