Restriction map for
pF1KB5192
(Circular) (Six-base) MAPSORT of: pF1KB5192.seq Check: 8660 from: 1 to: 4496
pF1KB5192 4496 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 452 1534 452
Size: 1082 3414
Acc65I G'GTAC_C
Cuts at: 1512 1512
Size: 4496
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 280 849 1119 2321 2753 3500 3768 276
Size: 4 569 270 1202 432 747 268 1004
Fragments arranged by size:
1202 1004 747 569 432 270 268 4
AflIII A'CryG_T
Cuts at: 1848 2952 3929 1848
Size: 1104 977 2415
Fragments arranged by size:
2415 1104 977
AgeI A'CCGG_T
Cuts at: 2884 2884
Size: 4496
AleI CACnn'nnGTG
Cuts at: 733 1183 733
Size: 450 4046
AlwNI CAG_nnn'CTG
Cuts at: 147 1229 1448 1801 3368 147
Size: 1082 219 353 1567 1275
Fragments arranged by size:
1567 1275 1082 353 219
ApaI G_GGCC'C
Cuts at: 614 742 614
Size: 128 4368
ApaLI G'TGCA_C
Cuts at: 3266 3266
Size: 4496
ApoI r'AATT_y
Cuts at: 130 1500 3849 4105 130
Size: 1370 2349 256 521
Fragments arranged by size:
2349 1370 521 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4496
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 241 1516 241
Size: 1275 3221
BamHI G'GATC_C
Cuts at: 1217 1521 1217
Size: 304 4192
BanI G'GyrC_C
Cuts at: 552 595 814 1247 1512 1926 2174 2209 552
Size: 43 219 433 265 414 248 35 2839
Fragments arranged by size:
2839 433 414 265 248 219 43 35
BanII G_rGCy'C
Cuts at: 614 742 1320 1351 1433 1510 2540 614
Size: 128 578 31 82 77 1030 2570
Fragments arranged by size:
2570 1030 578 128 82 77 31
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BbsI GAAGACnn'nnnn_
Cuts at: 390 597 390
Size: 207 4289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1255 1289 1255
Size: 34 4462
BciVI GTATCCnnnnn_n'
Cuts at: 2394 3155 4496 2394
Size: 761 1341 2394
Fragments arranged by size:
2394 1341 761
BclI T'GATC_A
Cuts at: 2016 2016
Size: 4496
BglI GCCn_nnn'nGGC
Cuts at: 505 910 505
Size: 405 4091
BglII A'GATC_T
Cuts at: 191 2011 4048 191
Size: 1820 2037 639
Fragments arranged by size:
2037 1820 639
BlpI GC'TnA_GC
Cuts at: 340 1432 1608 340
Size: 1092 176 3228
Fragments arranged by size:
3228 1092 176
Bme1580I G_kGCm'C
Cuts at: 486 557 598 614 742 817 1252 2121 2214
Size: 71 41 16 128 75 435 869 93
Cuts at: 2214 3270 486
Size: 1056 1712
Fragments arranged by size:
1712 1056 869 435 128 93 75 71
41 16
BmrI ACTGGGnnnn_n'
Cuts at: 1458 1708 1789 2124 4268 1458
Size: 250 81 335 2144 1686
Fragments arranged by size:
2144 1686 335 250 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 4496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 255
Size: 4496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1672 2551 3043 3341 1672
Size: 879 492 298 2827
Fragments arranged by size:
2827 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4200 4200
Size: 4496
BsaAI yAC'GTr
Cuts at: 1185 1849 1185
Size: 664 3832
BsaBI GATnn'nnATC
Cuts at: 6 2010 4047 6
Size: 2004 2037 455
Fragments arranged by size:
2037 2004 455
BsaHI Gr'CG_yC
Cuts at: 395 1927 2175 2877 4373 395
Size: 1532 248 702 1496 518
Fragments arranged by size:
1532 1496 702 518 248
BsaWI w'CCGG_w
Cuts at: 1199 1685 2206 2884 3158 3305 3716 1199
Size: 486 521 678 274 147 411 1979
Fragments arranged by size:
1979 678 521 486 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1333 1363 1333
Size: 30 4466
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 946 1399 220
Size: 726 453 3317
Fragments arranged by size:
3317 726 453
BseYI C'CCAG_C
Cuts at: 173 1436 1921 2679 3256 3752 173
Size: 1263 485 758 577 496 917
Fragments arranged by size:
1263 917 758 577 496 485
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 301
Size: 4496
BsiEI CG_ry'CG
Cuts at: 104 2038 2084 3292 104
Size: 1934 46 1208 1308
Fragments arranged by size:
1934 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1320 1433 1510 2288 2478 3270 1320
Size: 113 77 778 190 792 2546
Fragments arranged by size:
2546 792 778 190 113 77
BsmI GAATG_Cn'
Cuts at: 1071 1071
Size: 4496
BsmBI CGTCTCn'nnnn_
Cuts at: 1486 1486
Size: 4496
Bsp1286I G_dGCh'C
Cuts at: 486 557 598 614 742 817 1252 1320 1351
Size: 71 41 16 128 75 435 68 31
Cuts at: 1351 1433 1510 2121 2214 2288 2478 2540 3270
Size: 82 77 611 93 74 190 62 730
Cuts at: 3270 486
Size: 1712
Fragments arranged by size:
1712 730 611 435 190 128 93 82
77 75 74 71 68 62 41 31
16
BspEI T'CCGG_A
Cuts at: 1199 3716 1199
Size: 2517 1979
BspHI T'CATG_A
Cuts at: 3672 3672
Size: 4496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1034 1546 1760 2062 2443 1034
Size: 512 214 302 381 3087
Fragments arranged by size:
3087 512 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2788 2842 4326 4491 50
Size: 2738 54 1484 165 55
Fragments arranged by size:
2738 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2408 2408
Size: 4496
BsrFI r'CCGG_y
Cuts at: 668 2494 2675 2884 668
Size: 1826 181 209 2280
Fragments arranged by size:
2280 1826 209 181
BssSI C'ACGA_G
Cuts at: 358 2767 3125 358
Size: 2409 358 1729
Fragments arranged by size:
2409 1729 358
BstAPI GCAn_nnn'nTGC
Cuts at: 147 474 1873 147
Size: 327 1399 2770
Fragments arranged by size:
2770 1399 327
BstBI TT'CG_AA
Cuts at: 2857 2857
Size: 4496
BstEII G'GTnAC_C
Cuts at: 775 1101 775
Size: 326 4170
BstXI CCAn_nnnn'nTGG
Cuts at: 735 735
Size: 4496
BstYI r'GATC_y
Cuts at: 1 191 1217 1469 1521 1988 2011 2346 2592
Size: 190 1026 252 52 467 23 335 246
Cuts at: 2592 3604 3690 3702 4048 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1026 1012 467 449 346 335 252 246
190 86 52 23 12
BtgI C'CryG_G
Cuts at: 107 275 818 899 107
Size: 168 543 81 3704
Fragments arranged by size:
3704 543 168 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1258 2446 2627 2790 1258
Size: 1188 181 163 2964
Fragments arranged by size:
2964 1188 181 163
BtsI GCAGTG_nn'
Cuts at: 515 1739 515
Size: 1224 3272
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 257 292 257
Size: 35 4461
DraI TTT'AAA
Cuts at: 1495 1495
Size: 4496
DrdI GACnn_nn'nnGTC
Cuts at: 2202 3060 2202
Size: 858 3638
EaeI y'GGCC_r
Cuts at: 110 497 902 1322 2081 2255 2646 2673 4018
Size: 387 405 420 759 174 391 27 1345
Cuts at: 4018 110
Size: 588
Fragments arranged by size:
1345 759 588 420 405 391 387 174
27
EagI C'GGCC_G
Cuts at: 2081 2081
Size: 4496
EarI CTCTTCn'nnn_
Cuts at: 754 2519 2729 754
Size: 1765 210 2521
Fragments arranged by size:
2521 1765 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1063 2704 3014 3160 4306 1063
Size: 1641 310 146 1146 1253
Fragments arranged by size:
1641 1253 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1318 1431 1508 1318
Size: 113 77 4306
Fragments arranged by size:
4306 113 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 276 280 849 1119 2321 2753 3500 3768
Size: 21 4 569 270 1202 432 747 268
Cuts at: 3768 255
Size: 983
Fragments arranged by size:
1202 983 747 569 432 270 268 21
4
EcoO109I rG'GnC_Cy
Cuts at: 180 611 739 954 1082 1635 180
Size: 431 128 215 128 553 3041
Fragments arranged by size:
3041 553 431 215 128 128
EcoRI G'AATT_C
Cuts at: 1500 1500
Size: 4496
EcoRV GAT'ATC
Cuts at: 620 620
Size: 4496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4496
FspI TGC'GCA
Cuts at: 2277 2277
Size: 4496
HaeII r_GCGC'y
Cuts at: 1121 1209 1930 2178 3200 4179 1121
Size: 88 721 248 1022 979 1438
Fragments arranged by size:
1438 1022 979 721 248 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1454 1486 2338 2368 2370 2400 1454
Size: 32 852 30 2 30 3550
Fragments arranged by size:
3550 852 32 30 30 2
HincII GTy'rAC
Cuts at: 1535 1535
Size: 4496
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 1516 1516
Size: 4496
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3167 3351 3167
Size: 184 4312
MscI TGG'CCA
Cuts at: 112 499 1324 2257 112
Size: 387 825 933 2351
Fragments arranged by size:
2351 933 825 387
MslI CAynn'nnrTG
Cuts at: 733 1183 2485 2612 733
Size: 450 1302 127 2617
Fragments arranged by size:
2617 1302 450 127
MspA1I CmG'CkG
Cuts at: 315 1436 1607 1921 2281 3294 3539 315
Size: 1121 171 314 360 1013 245 1272
Fragments arranged by size:
1272 1121 1013 360 314 245 171
NaeI GCC'GGC
Cuts at: 2677 2677
Size: 4496
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NcoI C'CATG_G
Cuts at: 107 818 107
Size: 711 3785
NgoMIV G'CCGG_C
Cuts at: 2675 2675
Size: 4496
NspI r_CATG'y
Cuts at: 268 1195 1549 2049 2956 268
Size: 927 354 500 907 1808
Fragments arranged by size:
1808 927 907 500 354
PciI A'CATG_T
Cuts at: 2952 2952
Size: 4496
PflMI CCAn_nnn'nTGG
Cuts at: 1448 1448
Size: 4496
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4464
PpuMI rG'GwC_Cy
Cuts at: 954 1082 954
Size: 128 4368
PspOMI G'GGCC_C
Cuts at: 610 738 610
Size: 128 4368
PstI C_TGCA'G
Cuts at: 680 1543 2228 680
Size: 863 685 2948
Fragments arranged by size:
2948 863 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 315 1436 1921 2281 315
Size: 1121 485 360 2530
Fragments arranged by size:
2530 1121 485 360
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 1320 1433 1510 1320
Size: 113 77 4306
Fragments arranged by size:
4306 113 77
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SapI GCTCTTCn'nnn_
Cuts at: 2519 2729 2519
Size: 210 4286
SbfI CC_TGCA'GG
Cuts at: 1543 1543
Size: 4496
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SfcI C'TryA_G
Cuts at: 33 676 1539 2224 3217 3408 33
Size: 643 863 685 993 191 1121
Fragments arranged by size:
1121 993 863 685 643 191
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SmaI CCC'GGG
Cuts at: 1518 1518
Size: 4496
SmlI C'TyrA_G
Cuts at: 1651 2566 3058 3320 1651
Size: 915 492 262 2827
Fragments arranged by size:
2827 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4496
SphI G_CATG'C
Cuts at: 268 1549 2049 268
Size: 1281 500 2715
Fragments arranged by size:
2715 1281 500
StyI C'CwwG_G
Cuts at: 107 648 818 1630 1984 107
Size: 541 170 812 354 2619
Fragments arranged by size:
2619 812 541 354 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 424 983 2079 2212 2671 2880 424
Size: 559 1096 133 459 209 2040
Fragments arranged by size:
2040 1096 559 459 209 133
TatI w'GTAC_w
Cuts at: 4011 4011
Size: 4496
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1209 1241 4013 4045 1209
Size: 32 2772 32 1660
Fragments arranged by size:
2772 1660 32 32
Tth111I GACn'n_nGTC
Cuts at: 963 2293 963
Size: 1330 3166
XbaI T'CTAG_A
Cuts at: 1527 1527
Size: 4496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 816 906 1332 1991 816
Size: 90 426 659 3321
Fragments arranged by size:
3321 659 426 90
XmaI C'CCGG_G
Cuts at: 1516 1516
Size: 4496
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI
BsrGI BssHII BstZ17I Bsu36I ClaI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI