Restriction map for
pF1KB5186
(Circular) (Six-base) MAPSORT of: pF1KB5186.seq Check: 3021 from: 1 to: 3956
pF1KB5186 3956 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 320 994 320
Size: 674 3282
Acc65I G'GTAC_C
Cuts at: 972 972
Size: 3956
AclI AA'CG_TT
Cuts at: 3796 3796
Size: 3956
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1781 2213 2960 3228 1781
Size: 432 747 268 2509
Fragments arranged by size:
2509 747 432 268
AfeI AGC'GCT
Cuts at: 616 937 616
Size: 321 3635
AflIII A'CryG_T
Cuts at: 341 1308 2412 3389 341
Size: 967 1104 977 908
Fragments arranged by size:
1104 977 967 908
AgeI A'CCGG_T
Cuts at: 2344 2344
Size: 3956
AhdI GACnn_n'nnGTC
Cuts at: 480 480
Size: 3956
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 652 684 652
Size: 32 3924
AlwNI CAG_nnn'CTG
Cuts at: 1261 2828 1261
Size: 1567 2389
ApaLI G'TGCA_C
Cuts at: 2726 2726
Size: 3956
ApoI r'AATT_y
Cuts at: 960 3309 3565 960
Size: 2349 256 1351
Fragments arranged by size:
2349 1351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3956
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
AvaI C'yCGr_G
Cuts at: 141 822 976 141
Size: 681 154 3121
Fragments arranged by size:
3121 681 154
BamHI G'GATC_C
Cuts at: 981 981
Size: 3956
BanI G'GyrC_C
Cuts at: 374 846 972 1386 1634 1669 374
Size: 472 126 414 248 35 2661
Fragments arranged by size:
2661 472 414 248 126 35
BanII G_rGCy'C
Cuts at: 715 970 2000 715
Size: 255 1030 2671
Fragments arranged by size:
2671 1030 255
BbeI G_GCGC'C
Cuts at: 378 1390 1638 378
Size: 1012 248 2696
Fragments arranged by size:
2696 1012 248
BciVI GTATCCnnnnn_n'
Cuts at: 172 1854 2615 3956 172
Size: 1682 761 1341 172
Fragments arranged by size:
1682 1341 761 172
BclI T'GATC_A
Cuts at: 125 1476 125
Size: 1351 2605
BglII A'GATC_T
Cuts at: 632 1471 3508 632
Size: 839 2037 1080
Fragments arranged by size:
2037 1080 839
BlpI GC'TnA_GC
Cuts at: 1068 1068
Size: 3956
Bme1580I G_kGCm'C
Cuts at: 142 524 1581 1674 2730 142
Size: 382 1057 93 1056 1368
Fragments arranged by size:
1368 1057 1056 382 93
BmgBI CAC'GTC
Cuts at: 344 519 753 344
Size: 175 234 3547
Fragments arranged by size:
3547 234 175
BmrI ACTGGGnnnn_n'
Cuts at: 1168 1249 1584 3728 1168
Size: 81 335 2144 1396
Fragments arranged by size:
2144 1396 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2323 2323
Size: 3956
Bpu10I CC'TnA_GC
Cuts at: 457 457
Size: 3956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 1132 2011 2503 2801 475
Size: 657 879 492 298 1630
Fragments arranged by size:
1630 879 657 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 738 3660 738
Size: 2922 1034
BsaAI yAC'GTr
Cuts at: 1309 1309
Size: 3956
BsaBI GATnn'nnATC
Cuts at: 6 1470 3507 6
Size: 1464 2037 455
Fragments arranged by size:
2037 1464 455
BsaHI Gr'CG_yC
Cuts at: 260 375 830 1387 1635 2337 3833 260
Size: 115 455 557 248 702 1496 383
Fragments arranged by size:
1496 702 557 455 383 248 115
BsaWI w'CCGG_w
Cuts at: 1145 1666 2344 2618 2765 3176 1145
Size: 521 678 274 147 411 1925
Fragments arranged by size:
1925 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 418 448 418
Size: 30 3926
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 457 457
Size: 3956
BseYI C'CCAG_C
Cuts at: 611 700 865 1381 2139 2716 3212 611
Size: 89 165 516 758 577 496 1355
Fragments arranged by size:
1355 758 577 516 496 165 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 706 742 760 469
Size: 237 36 18 3665
Fragments arranged by size:
3665 237 36 18
BsiEI CG_ry'CG
Cuts at: 104 249 1498 1544 2752 104
Size: 145 1249 46 1208 1308
Fragments arranged by size:
1308 1249 1208 145 46
BsiHKAI G_wGCw'C
Cuts at: 970 1748 1938 2730 970
Size: 778 190 792 2196
Fragments arranged by size:
2196 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 389 389
Size: 3956
Bsp1286I G_dGCh'C
Cuts at: 142 524 715 970 1581 1674 1748 1938 2000
Size: 382 191 255 611 93 74 190 62
Cuts at: 2000 2730 142
Size: 730 1368
Fragments arranged by size:
1368 730 611 382 255 191 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3176 3176
Size: 3956
BspHI T'CATG_A
Cuts at: 3132 3132
Size: 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 728 1006 1220 1522 1903 728
Size: 278 214 302 381 2781
Fragments arranged by size:
2781 381 302 278 214
BsrBI CCG'CTC
Cuts at: 50 2248 2302 3786 3951 50
Size: 2198 54 1484 165 55
Fragments arranged by size:
2198 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1868 1868
Size: 3956
BsrFI r'CCGG_y
Cuts at: 244 796 860 910 1954 2135 2344 244
Size: 552 64 50 1044 181 209 1856
Fragments arranged by size:
1856 1044 552 209 181 64 50
BsrGI T'GTAC_A
Cuts at: 887 887
Size: 3956
BssHII G'CGCG_C
Cuts at: 674 856 674
Size: 182 3774
BssSI C'ACGA_G
Cuts at: 2227 2585 2227
Size: 358 3598
BstAPI GCAn_nnn'nTGC
Cuts at: 1333 1333
Size: 3956
BstBI TT'CG_AA
Cuts at: 2317 2317
Size: 3956
BstEII G'GTnAC_C
Cuts at: 547 547
Size: 3956
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3956
BstYI r'GATC_y
Cuts at: 1 467 632 981 1448 1471 1806 2052 3064
Size: 466 165 349 467 23 335 246 1012
Cuts at: 3064 3150 3162 3508 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 466 449 349 346 335 246
165 86 23 12
BtgI C'CryG_G
Cuts at: 309 686 309
Size: 377 3579
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 136 1906 2087 2250 136
Size: 1770 181 163 1842
Fragments arranged by size:
1842 1770 181 163
BtsI GCAGTG_nn'
Cuts at: 1199 1199
Size: 3956
DraI TTT'AAA
Cuts at: 955 955
Size: 3956
DrdI GACnn_nn'nnGTC
Cuts at: 1662 2520 1662
Size: 858 3098
EaeI y'GGCC_r
Cuts at: 116 167 221 246 638 689 1541 1715 2106
Size: 51 54 25 392 51 852 174 391
Cuts at: 2106 2133 3478 116
Size: 27 1345 594
Fragments arranged by size:
1345 852 594 392 391 174 54 51
51 27 25
EagI C'GGCC_G
Cuts at: 246 1541 246
Size: 1295 2661
EarI CTCTTCn'nnn_
Cuts at: 685 1979 2189 685
Size: 1294 210 2452
Fragments arranged by size:
2452 1294 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2164 2474 2620 3766 2164
Size: 310 146 1146 2354
Fragments arranged by size:
2354 1146 310 146
EcoICRI GAG'CTC
Cuts at: 968 968
Size: 3956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1781 2213 2960 3228 1781
Size: 432 747 268 2509
Fragments arranged by size:
2509 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1095 1095
Size: 3956
EcoRI G'AATT_C
Cuts at: 960 960
Size: 3956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1975 1975
Size: 3956
FspI TGC'GCA
Cuts at: 1737 1737
Size: 3956
HaeII r_GCGC'y
Cuts at: 378 618 772 911 939 1390 1638 2660 3639
Size: 240 154 139 28 451 248 1022 979
Cuts at: 3639 378
Size: 695
Fragments arranged by size:
1022 979 695 451 248 240 154 139
28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 466 498 1798 1828 1830 1860 466
Size: 32 1300 30 2 30 2562
Fragments arranged by size:
2562 1300 32 30 30 2
HincII GTy'rAC
Cuts at: 995 995
Size: 3956
KasI G'GCGC_C
Cuts at: 374 1386 1634 374
Size: 1012 248 2696
Fragments arranged by size:
2696 1012 248
KpnI G_GTAC'C
Cuts at: 976 976
Size: 3956
MluI A'CGCG_T
Cuts at: 3389 3389
Size: 3956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2627 2811 2627
Size: 184 3772
MscI TGG'CCA
Cuts at: 691 1717 691
Size: 1026 2930
MslI CAynn'nnrTG
Cuts at: 409 1945 2072 409
Size: 1536 127 2293
Fragments arranged by size:
2293 1536 127
MspA1I CmG'CkG
Cuts at: 311 453 594 803 1067 1381 1741 2754 2999
Size: 142 141 209 264 314 360 1013 245
Cuts at: 2999 311
Size: 1268
Fragments arranged by size:
1268 1013 360 314 264 245 209 142
141
NaeI GCC'GGC
Cuts at: 798 862 912 2137 798
Size: 64 50 1225 2617
Fragments arranged by size:
2617 1225 64 50
NarI GG'CG_CC
Cuts at: 375 1387 1635 375
Size: 1012 248 2696
Fragments arranged by size:
2696 1012 248
NcoI C'CATG_G
Cuts at: 686 686
Size: 3956
NgoMIV G'CCGG_C
Cuts at: 796 860 910 2135 796
Size: 64 50 1225 2617
Fragments arranged by size:
2617 1225 64 50
NspI r_CATG'y
Cuts at: 1009 1509 2416 1009
Size: 500 907 2549
Fragments arranged by size:
2549 907 500
PciI A'CATG_T
Cuts at: 2412 2412
Size: 3956
PmeI GTTT'AAAC
Cuts at: 955 955
Size: 3956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3095 3127 3095
Size: 32 3924
PstI C_TGCA'G
Cuts at: 1003 1688 1003
Size: 685 3271
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3956
PvuII CAG'CTG
Cuts at: 594 1381 1741 594
Size: 787 360 2809
Fragments arranged by size:
2809 787 360
RsrII CG'GwC_CG
Cuts at: 2151 2151
Size: 3956
SacI G_AGCT'C
Cuts at: 970 970
Size: 3956
SacII CC_GC'GG
Cuts at: 312 312
Size: 3956
SalI G'TCGA_C
Cuts at: 993 993
Size: 3956
SapI GCTCTTCn'nnn_
Cuts at: 685 1979 2189 685
Size: 1294 210 2452
Fragments arranged by size:
2452 1294 210
SbfI CC_TGCA'GG
Cuts at: 1003 1003
Size: 3956
ScaI AGT'ACT
Cuts at: 745 3473 745
Size: 2728 1228
SfcI C'TryA_G
Cuts at: 33 999 1684 2677 2868 33
Size: 966 685 993 191 1121
Fragments arranged by size:
1121 993 966 685 191
SfoI GGC'GCC
Cuts at: 376 1388 1636 376
Size: 1012 248 2696
Fragments arranged by size:
2696 1012 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
SmaI CCC'GGG
Cuts at: 824 978 824
Size: 154 3802
SmlI C'TyrA_G
Cuts at: 490 1111 2026 2518 2780 490
Size: 621 915 492 262 1666
Fragments arranged by size:
1666 915 621 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3956
SphI G_CATG'C
Cuts at: 1009 1509 1009
Size: 500 3456
StyI C'CwwG_G
Cuts at: 206 503 686 923 1090 1444 206
Size: 297 183 237 167 354 2718
Fragments arranged by size:
2718 354 297 237 183 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1539 1672 2131 2340 1539
Size: 133 459 209 3155
Fragments arranged by size:
3155 459 209 133
TatI w'GTAC_w
Cuts at: 743 887 3471 743
Size: 144 2584 1228
Fragments arranged by size:
2584 1228 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 3924
Tth111I GACn'n_nGTC
Cuts at: 1753 1753
Size: 3956
XbaI T'CTAG_A
Cuts at: 987 987
Size: 3956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 510 1451 510
Size: 941 3015
XmaI C'CCGG_G
Cuts at: 822 976 822
Size: 154 3802
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI BpmI
BsiWI BsmI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI