Restriction map for
pF1KB5169
(Circular) (Six-base) MAPSORT of: pF1KB5169.seq Check: 9929 from: 1 to: 4424
pF1KB5169 4424 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 512 1462 512
Size: 950 3474
Acc65I G'GTAC_C
Cuts at: 700 1440 700
Size: 740 3684
AclI AA'CG_TT
Cuts at: 4264 4264
Size: 4424
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2249 2681 3428 3696 2249
Size: 432 747 268 2977
Fragments arranged by size:
2977 747 432 268
AflIII A'CryG_T
Cuts at: 1343 1776 2880 3857 1343
Size: 433 1104 977 1910
Fragments arranged by size:
1910 1104 977 433
AgeI A'CCGG_T
Cuts at: 2812 2812
Size: 4424
AlwNI CAG_nnn'CTG
Cuts at: 120 1729 3296 120
Size: 1609 1567 1248
Fragments arranged by size:
1609 1567 1248
ApaI G_GGCC'C
Cuts at: 1005 1005
Size: 4424
ApaLI G'TGCA_C
Cuts at: 3194 3194
Size: 4424
ApoI r'AATT_y
Cuts at: 1428 3777 4033 1428
Size: 2349 256 1819
Fragments arranged by size:
2349 1819 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4424
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
AvaI C'yCGr_G
Cuts at: 260 293 884 1111 1283 1444 260
Size: 33 591 227 172 161 3240
Fragments arranged by size:
3240 591 227 172 161 33
BamHI G'GATC_C
Cuts at: 1449 1449
Size: 4424
BanI G'GyrC_C
Cuts at: 700 1199 1440 1854 2102 2137 700
Size: 499 241 414 248 35 2987
Fragments arranged by size:
2987 499 414 248 241 35
BanII G_rGCy'C
Cuts at: 1005 1064 1112 1438 2468 1005
Size: 59 48 326 1030 2961
Fragments arranged by size:
2961 1030 326 59 48
BbeI G_GCGC'C
Cuts at: 1203 1858 2106 1203
Size: 655 248 3521
Fragments arranged by size:
3521 655 248
BciVI GTATCCnnnnn_n'
Cuts at: 2322 3083 4424 2322
Size: 761 1341 2322
Fragments arranged by size:
2322 1341 761
BclI T'GATC_A
Cuts at: 1944 1944
Size: 4424
BglI GCCn_nnn'nGGC
Cuts at: 678 678
Size: 4424
BglII A'GATC_T
Cuts at: 1939 3976 1939
Size: 2037 2387
BlpI GC'TnA_GC
Cuts at: 1536 1536
Size: 4424
Bme1580I G_kGCm'C
Cuts at: 1005 2049 2142 3198 1005
Size: 1044 93 1056 2231
Fragments arranged by size:
2231 1056 1044 93
BmgBI CAC'GTC
Cuts at: 639 639
Size: 4424
BmrI ACTGGGnnnn_n'
Cuts at: 108 1636 1717 2052 4196 108
Size: 1528 81 335 2144 336
Fragments arranged by size:
2144 1528 336 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 709 2791 709
Size: 2082 2342
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 687 921 1085 1336 202
Size: 485 234 164 251 3290
Fragments arranged by size:
3290 485 251 234 164
Bpu10I CC'TnA_GC
Cuts at: 398 398
Size: 4424
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1600 2479 2971 3269 1600
Size: 879 492 298 2755
Fragments arranged by size:
2755 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4128 4128
Size: 4424
BsaAI yAC'GTr
Cuts at: 1777 1777
Size: 4424
BsaBI GATnn'nnATC
Cuts at: 6 1938 3975 6
Size: 1932 2037 455
Fragments arranged by size:
2037 1932 455
BsaHI Gr'CG_yC
Cuts at: 1200 1855 2103 2805 4301 1200
Size: 655 248 702 1496 1323
Fragments arranged by size:
1496 1323 702 655 248
BsaWI w'CCGG_w
Cuts at: 464 968 1181 1383 1613 2134 2812 3086 3233
Size: 504 213 202 230 521 678 274 147
Cuts at: 3233 3644 464
Size: 411 1244
Fragments arranged by size:
1244 678 521 504 411 274 230 213
202 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 565 595 1378 1408 565
Size: 30 783 30 3581
Fragments arranged by size:
3581 783 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 263 313 701 725 937 1042 263
Size: 50 388 24 212 105 3645
Fragments arranged by size:
3645 388 212 105 50 24
BseYI C'CCAG_C
Cuts at: 1849 2607 3184 3680 1849
Size: 758 577 496 2593
Fragments arranged by size:
2593 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1966 2012 3220 104
Size: 1862 46 1208 1308
Fragments arranged by size:
1862 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1112 1241 1438 2216 2406 3198 1112
Size: 129 197 778 190 792 2338
Fragments arranged by size:
2338 792 778 197 190 129
BsmI GAATG_Cn'
Cuts at: 735 735
Size: 4424
Bsp1286I G_dGCh'C
Cuts at: 1005 1064 1112 1241 1438 2049 2142 2216 2406
Size: 59 48 129 197 611 93 74 190
Cuts at: 2406 2468 3198 1005
Size: 62 730 2231
Fragments arranged by size:
2231 730 611 197 190 129 93 74
62 59 48
BspEI T'CCGG_A
Cuts at: 464 1383 3644 464
Size: 919 2261 1244
Fragments arranged by size:
2261 1244 919
BspHI T'CATG_A
Cuts at: 3600 3600
Size: 4424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 179 1474 1688 1990 2371 179
Size: 1295 214 302 381 2232
Fragments arranged by size:
2232 1295 381 302 214
BsrBI CCG'CTC
Cuts at: 50 336 822 837 881 927 945 999 1014
Size: 286 486 15 44 46 18 54 15
Cuts at: 1014 1197 1254 1266 2716 2770 4254 4419 50
Size: 183 57 12 1450 54 1484 165 55
Fragments arranged by size:
1484 1450 486 286 183 165 57 55
54 54 46 44 18 15 15 12
BsrDI GCAATG_nn'
Cuts at: 227 2336 227
Size: 2109 2315
BsrFI r'CCGG_y
Cuts at: 890 2422 2603 2812 890
Size: 1532 181 209 2502
Fragments arranged by size:
2502 1532 209 181
BsrGI T'GTAC_A
Cuts at: 1346 1346
Size: 4424
BssSI C'ACGA_G
Cuts at: 281 565 2695 3053 281
Size: 284 2130 358 1652
Fragments arranged by size:
2130 1652 358 284
BstAPI GCAn_nnn'nTGC
Cuts at: 1801 1801
Size: 4424
BstBI TT'CG_AA
Cuts at: 2785 2785
Size: 4424
BstXI CCAn_nnnn'nTGG
Cuts at: 135 135
Size: 4424
BstYI r'GATC_y
Cuts at: 1 1449 1916 1939 2274 2520 3532 3618 3630
Size: 1448 467 23 335 246 1012 86 12
Cuts at: 3630 3976 1
Size: 346 449
Fragments arranged by size:
1448 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 537 537
Size: 4424
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 761 2374 2555 2718 761
Size: 1613 181 163 2467
Fragments arranged by size:
2467 1613 181 163
BtsI GCAGTG_nn'
Cuts at: 1266 1667 1266
Size: 401 4023
DraI TTT'AAA
Cuts at: 1423 1423
Size: 4424
DrdI GACnn_nn'nnGTC
Cuts at: 2130 2988 2130
Size: 858 3566
EaeI y'GGCC_r
Cuts at: 1299 2009 2183 2574 2601 3946 1299
Size: 710 174 391 27 1345 1777
Fragments arranged by size:
1777 1345 710 391 174 27
EagI C'GGCC_G
Cuts at: 2009 2009
Size: 4424
EarI CTCTTCn'nnn_
Cuts at: 427 702 2447 2657 427
Size: 275 1745 210 2194
Fragments arranged by size:
2194 1745 275 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 909 1072 1188 2632 2942 3088 4234 909
Size: 163 116 1444 310 146 1146 1099
Fragments arranged by size:
1444 1146 1099 310 163 146 116
EcoICRI GAG'CTC
Cuts at: 1110 1436 1110
Size: 326 4098
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 687 921 1085 1336 2249 2681 3428 3696
Size: 485 234 164 251 913 432 747 268
Cuts at: 3696 202
Size: 930
Fragments arranged by size:
930 913 747 485 432 268 251 234
164
EcoO109I rG'GnC_Cy
Cuts at: 255 375 627 671 1563 255
Size: 120 252 44 892 3116
Fragments arranged by size:
3116 892 252 120 44
EcoRI G'AATT_C
Cuts at: 1428 1428
Size: 4424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 438 2443 438
Size: 2005 2419
FspI TGC'GCA
Cuts at: 2205 2205
Size: 4424
HaeII r_GCGC'y
Cuts at: 903 936 1203 1858 2106 3128 4107 903
Size: 33 267 655 248 1022 979 1220
Fragments arranged by size:
1220 1022 979 655 267 248 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 565 597 739 771 2266 2296 2298 2328 565
Size: 32 142 32 1495 30 2 30 2661
Fragments arranged by size:
2661 1495 142 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1463 1463
Size: 4424
KasI G'GCGC_C
Cuts at: 1199 1854 2102 1199
Size: 655 248 3521
Fragments arranged by size:
3521 655 248
KpnI G_GTAC'C
Cuts at: 704 1444 704
Size: 740 3684
MluI A'CGCG_T
Cuts at: 3857 3857
Size: 4424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 301 3095 3279 301
Size: 2794 184 1446
Fragments arranged by size:
2794 1446 184
MscI TGG'CCA
Cuts at: 1301 2185 1301
Size: 884 3540
MslI CAynn'nnrTG
Cuts at: 599 1348 2413 2540 599
Size: 749 1065 127 2483
Fragments arranged by size:
2483 1065 749 127
MspA1I CmG'CkG
Cuts at: 1535 1849 2209 3222 3467 1535
Size: 314 360 1013 245 2492
Fragments arranged by size:
2492 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2605 2605
Size: 4424
NarI GG'CG_CC
Cuts at: 1200 1855 2103 1200
Size: 655 248 3521
Fragments arranged by size:
3521 655 248
NgoMIV G'CCGG_C
Cuts at: 2603 2603
Size: 4424
NruI TCG'CGA
Cuts at: 914 914
Size: 4424
NspI r_CATG'y
Cuts at: 579 1347 1477 1977 2884 579
Size: 768 130 500 907 2119
Fragments arranged by size:
2119 907 768 500 130
PciI A'CATG_T
Cuts at: 1343 2880 1343
Size: 1537 2887
PmeI GTTT'AAAC
Cuts at: 1423 1423
Size: 4424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 4392
PpuMI rG'GwC_Cy
Cuts at: 255 375 627 255
Size: 120 252 4052
Fragments arranged by size:
4052 252 120
PspOMI G'GGCC_C
Cuts at: 1001 1001
Size: 4424
PstI C_TGCA'G
Cuts at: 1471 2156 1471
Size: 685 3739
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4424
PvuII CAG'CTG
Cuts at: 1849 2209 1849
Size: 360 4064
RsrII CG'GwC_CG
Cuts at: 2619 2619
Size: 4424
SacI G_AGCT'C
Cuts at: 1112 1438 1112
Size: 326 4098
SalI G'TCGA_C
Cuts at: 1461 1461
Size: 4424
SanDI GG'GwC_CC
Cuts at: 375 375
Size: 4424
SapI GCTCTTCn'nnn_
Cuts at: 2447 2657 2447
Size: 210 4214
SbfI CC_TGCA'GG
Cuts at: 1471 1471
Size: 4424
ScaI AGT'ACT
Cuts at: 3941 3941
Size: 4424
SfcI C'TryA_G
Cuts at: 33 513 1467 2152 3145 3336 33
Size: 480 954 685 993 191 1121
Fragments arranged by size:
1121 993 954 685 480 191
SfoI GGC'GCC
Cuts at: 1201 1856 2104 1201
Size: 655 248 3521
Fragments arranged by size:
3521 655 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
SmaI CCC'GGG
Cuts at: 886 1446 886
Size: 560 3864
SmlI C'TyrA_G
Cuts at: 260 1579 2494 2986 3248 260
Size: 1319 915 492 262 1436
Fragments arranged by size:
1436 1319 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4424
SphI G_CATG'C
Cuts at: 1477 1977 1477
Size: 500 3924
StuI AGG'CCT
Cuts at: 692 692
Size: 4424
StyI C'CwwG_G
Cuts at: 1558 1912 1558
Size: 354 4070
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2007 2140 2599 2808 2007
Size: 133 459 209 3623
Fragments arranged by size:
3623 459 209 133
TatI w'GTAC_w
Cuts at: 1346 3939 1346
Size: 2593 1831
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3941 3973 3941
Size: 32 4392
Tth111I GACn'n_nGTC
Cuts at: 2221 2221
Size: 4424
XbaI T'CTAG_A
Cuts at: 1455 1455
Size: 4424
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1919 1919
Size: 4424
XhoI C'TCGA_G
Cuts at: 260 260
Size: 4424
XmaI C'CCGG_G
Cuts at: 884 1444 884
Size: 560 3864
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NruI NspI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI
BsgI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI
ZraI