Restriction map for
pF1KB5163
(Circular) (Six-base) MAPSORT of: pF1KB5163.seq Check: 2098 from: 1 to: 3737
pF1KB5163 3737 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 775 775
Size: 3737
Acc65I G'GTAC_C
Cuts at: 753 753
Size: 3737
AclI AA'CG_TT
Cuts at: 3577 3577
Size: 3737
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1562 1994 2741 3009 1562
Size: 432 747 268 2290
Fragments arranged by size:
2290 747 432 268
AflIII A'CryG_T
Cuts at: 612 1089 2193 3170 612
Size: 477 1104 977 1179
Fragments arranged by size:
1179 1104 977 477
AgeI A'CCGG_T
Cuts at: 2125 2125
Size: 3737
AlwNI CAG_nnn'CTG
Cuts at: 1042 2609 1042
Size: 1567 2170
ApaLI G'TGCA_C
Cuts at: 2507 2507
Size: 3737
ApoI r'AATT_y
Cuts at: 145 741 3090 3346 145
Size: 596 2349 256 536
Fragments arranged by size:
2349 596 536 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3737
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3737
AvaI C'yCGr_G
Cuts at: 757 757
Size: 3737
BamHI G'GATC_C
Cuts at: 762 762
Size: 3737
BanI G'GyrC_C
Cuts at: 753 1167 1415 1450 753
Size: 414 248 35 3040
Fragments arranged by size:
3040 414 248 35
BanII G_rGCy'C
Cuts at: 751 1781 751
Size: 1030 2707
BbeI G_GCGC'C
Cuts at: 1171 1419 1171
Size: 248 3489
BbvCI CC'TCA_GC
Cuts at: 139 139
Size: 3737
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 589 623 589
Size: 34 3703
BciVI GTATCCnnnnn_n'
Cuts at: 580 1635 2396 3737 580
Size: 1055 761 1341 580
Fragments arranged by size:
1341 1055 761 580
BclI T'GATC_A
Cuts at: 194 1257 194
Size: 1063 2674
BglII A'GATC_T
Cuts at: 492 1252 3289 492
Size: 760 2037 940
Fragments arranged by size:
2037 940 760
BlpI GC'TnA_GC
Cuts at: 849 849
Size: 3737
Bme1580I G_kGCm'C
Cuts at: 1362 1455 2511 1362
Size: 93 1056 2588
Fragments arranged by size:
2588 1056 93
BmgBI CAC'GTC
Cuts at: 613 613
Size: 3737
BmrI ACTGGGnnnn_n'
Cuts at: 949 1030 1365 3509 949
Size: 81 335 2144 1177
Fragments arranged by size:
2144 1177 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 3737
Bpu10I CC'TnA_GC
Cuts at: 139 139
Size: 3737
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 913 1792 2284 2582 913
Size: 879 492 298 2068
Fragments arranged by size:
2068 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3441 3441
Size: 3737
BsaAI yAC'GTr
Cuts at: 1090 1090
Size: 3737
BsaBI GATnn'nnATC
Cuts at: 6 1251 3288 6
Size: 1245 2037 455
Fragments arranged by size:
2037 1245 455
BsaHI Gr'CG_yC
Cuts at: 1168 1416 2118 3614 1168
Size: 248 702 1496 1291
Fragments arranged by size:
1496 1291 702 248
BsaWI w'CCGG_w
Cuts at: 926 1447 2125 2399 2546 2957 926
Size: 521 678 274 147 411 1706
Fragments arranged by size:
1706 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 538 538
Size: 3737
BseYI C'CCAG_C
Cuts at: 1162 1920 2497 2993 1162
Size: 758 577 496 1906
Fragments arranged by size:
1906 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1279 1325 2533 104
Size: 1175 46 1208 1308
Fragments arranged by size:
1308 1208 1175 46
BsiHKAI G_wGCw'C
Cuts at: 751 1529 1719 2511 751
Size: 778 190 792 1977
Fragments arranged by size:
1977 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 751 1362 1455 1529 1719 1781 2511 751
Size: 611 93 74 190 62 730 1977
Fragments arranged by size:
1977 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2957 2957
Size: 3737
BspHI T'CATG_A
Cuts at: 2913 2913
Size: 3737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 307 787 1001 1303 1684 307
Size: 480 214 302 381 2360
Fragments arranged by size:
2360 480 381 302 214
BsrBI CCG'CTC
Cuts at: 50 327 2029 2083 3567 3732 50
Size: 277 1702 54 1484 165 55
Fragments arranged by size:
1702 1484 277 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1649 1649
Size: 3737
BsrFI r'CCGG_y
Cuts at: 1735 1916 2125 1735
Size: 181 209 3347
Fragments arranged by size:
3347 209 181
BssSI C'ACGA_G
Cuts at: 2008 2366 2008
Size: 358 3379
BstAPI GCAn_nnn'nTGC
Cuts at: 1114 1114
Size: 3737
BstBI TT'CG_AA
Cuts at: 2098 2098
Size: 3737
BstYI r'GATC_y
Cuts at: 1 492 762 1229 1252 1587 1833 2845 2931
Size: 491 270 467 23 335 246 1012 86
Cuts at: 2931 2943 3289 1
Size: 12 346 449
Fragments arranged by size:
1012 491 467 449 346 335 270 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 695 695
Size: 3737
BtgI C'CryG_G
Cuts at: 113 113
Size: 3737
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1687 1868 2031 1687
Size: 181 163 3393
Fragments arranged by size:
3393 181 163
BtsI GCAGTG_nn'
Cuts at: 980 980
Size: 3737
DraI TTT'AAA
Cuts at: 736 736
Size: 3737
DrdI GACnn_nn'nnGTC
Cuts at: 1443 2301 1443
Size: 858 2879
EaeI y'GGCC_r
Cuts at: 1322 1496 1887 1914 3259 1322
Size: 174 391 27 1345 1800
Fragments arranged by size:
1800 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1322 1322
Size: 3737
EarI CTCTTCn'nnn_
Cuts at: 1760 1970 1760
Size: 210 3527
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 134 1945 2255 2401 3547 134
Size: 1811 310 146 1146 324
Fragments arranged by size:
1811 1146 324 310 146
EcoICRI GAG'CTC
Cuts at: 749 749
Size: 3737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1562 1994 2741 3009 1562
Size: 432 747 268 2290
Fragments arranged by size:
2290 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 876 876
Size: 3737
EcoRI G'AATT_C
Cuts at: 741 741
Size: 3737
EcoRV GAT'ATC
Cuts at: 390 390
Size: 3737
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 621 1756 621
Size: 1135 2602
FspI TGC'GCA
Cuts at: 1518 1518
Size: 3737
HaeII r_GCGC'y
Cuts at: 1171 1419 2441 3420 1171
Size: 248 1022 979 1488
Fragments arranged by size:
1488 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 244 276 1579 1609 1611 1641 244
Size: 32 1303 30 2 30 2340
Fragments arranged by size:
2340 1303 32 30 30 2
HincII GTy'rAC
Cuts at: 402 776 402
Size: 374 3363
HpaI GTT'AAC
Cuts at: 402 402
Size: 3737
KasI G'GCGC_C
Cuts at: 1167 1415 1167
Size: 248 3489
KpnI G_GTAC'C
Cuts at: 757 757
Size: 3737
MfeI C'AATT_G
Cuts at: 242 518 242
Size: 276 3461
MluI A'CGCG_T
Cuts at: 3170 3170
Size: 3737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 159 2408 2592 159
Size: 2249 184 1304
Fragments arranged by size:
2249 1304 184
MscI TGG'CCA
Cuts at: 1498 1498
Size: 3737
MslI CAynn'nnrTG
Cuts at: 1726 1853 1726
Size: 127 3610
MspA1I CmG'CkG
Cuts at: 138 848 1162 1522 2535 2780 138
Size: 710 314 360 1013 245 1095
Fragments arranged by size:
1095 1013 710 360 314 245
NaeI GCC'GGC
Cuts at: 1918 1918
Size: 3737
NarI GG'CG_CC
Cuts at: 1168 1416 1168
Size: 248 3489
NgoMIV G'CCGG_C
Cuts at: 1916 1916
Size: 3737
NspI r_CATG'y
Cuts at: 790 1290 2197 790
Size: 500 907 2330
Fragments arranged by size:
2330 907 500
PciI A'CATG_T
Cuts at: 2193 2193
Size: 3737
PmeI GTTT'AAAC
Cuts at: 736 736
Size: 3737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2876 2908 2876
Size: 32 3705
PstI C_TGCA'G
Cuts at: 784 1469 784
Size: 685 3052
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3737
PvuII CAG'CTG
Cuts at: 1162 1522 1162
Size: 360 3377
RsrII CG'GwC_CG
Cuts at: 1932 1932
Size: 3737
SacI G_AGCT'C
Cuts at: 751 751
Size: 3737
SalI G'TCGA_C
Cuts at: 774 774
Size: 3737
SapI GCTCTTCn'nnn_
Cuts at: 1760 1970 1760
Size: 210 3527
SbfI CC_TGCA'GG
Cuts at: 784 784
Size: 3737
ScaI AGT'ACT
Cuts at: 3254 3254
Size: 3737
SfcI C'TryA_G
Cuts at: 33 780 1465 2458 2649 33
Size: 747 685 993 191 1121
Fragments arranged by size:
1121 993 747 685 191
SfoI GGC'GCC
Cuts at: 1169 1417 1169
Size: 248 3489
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3737
SmaI CCC'GGG
Cuts at: 759 759
Size: 3737
SmlI C'TyrA_G
Cuts at: 892 1807 2299 2561 892
Size: 915 492 262 2068
Fragments arranged by size:
2068 915 492 262
SpeI A'CTAG_T
Cuts at: 67 571 67
Size: 504 3233
SphI G_CATG'C
Cuts at: 790 1290 790
Size: 500 3237
StyI C'CwwG_G
Cuts at: 871 1225 871
Size: 354 3383
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1320 1453 1912 2121 1320
Size: 133 459 209 2936
Fragments arranged by size:
2936 459 209 133
TatI w'GTAC_w
Cuts at: 272 3252 272
Size: 2980 757
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3254 3286 3254
Size: 32 3705
Tth111I GACn'n_nGTC
Cuts at: 1534 1534
Size: 3737
XbaI T'CTAG_A
Cuts at: 768 768
Size: 3737
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1232 1232
Size: 3737
XmaI C'CCGG_G
Cuts at: 757 757
Size: 3737
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BfrBI BglI BmtI
BpmI BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI