Restriction map for
pF1KB5161
(Circular) (Six-base) MAPSORT of: pF1KB5161.seq Check: 1 from: 1 to: 4502
pF1KB5161 4502 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 1540 1540
Size: 4502
Acc65I G'GTAC_C
Cuts at: 1518 1518
Size: 4502
AclI AA'CG_TT
Cuts at: 4342 4342
Size: 4502
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 728 1187 2327 2759 3506 3774 728
Size: 459 1140 432 747 268 1456
Fragments arranged by size:
1456 1140 747 459 432 268
AflIII A'CryG_T
Cuts at: 1467 1854 2958 3935 1467
Size: 387 1104 977 2034
Fragments arranged by size:
2034 1104 977 387
AgeI A'CCGG_T
Cuts at: 2890 2890
Size: 4502
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 749 781 749
Size: 32 4470
AlwNI CAG_nnn'CTG
Cuts at: 201 1807 3374 201
Size: 1606 1567 1329
Fragments arranged by size:
1606 1567 1329
ApaLI G'TGCA_C
Cuts at: 3272 3272
Size: 4502
ApoI r'AATT_y
Cuts at: 1480 1506 3855 4111 1480
Size: 26 2349 256 1871
Fragments arranged by size:
2349 1871 256 26
AseI AT'TA_AT
Cuts at: 20 652 20
Size: 632 3870
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
AvaI C'yCGr_G
Cuts at: 1522 1522
Size: 4502
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1328 1361 1328
Size: 33 4469
BamHI G'GATC_C
Cuts at: 1527 1527
Size: 4502
BanI G'GyrC_C
Cuts at: 1518 1932 2180 2215 1518
Size: 414 248 35 3805
Fragments arranged by size:
3805 414 248 35
BanII G_rGCy'C
Cuts at: 1516 2546 1516
Size: 1030 3472
BbeI G_GCGC'C
Cuts at: 1936 2184 1936
Size: 248 4254
BciVI GTATCCnnnnn_n'
Cuts at: 2400 3161 4502 2400
Size: 761 1341 2400
Fragments arranged by size:
2400 1341 761
BclI T'GATC_A
Cuts at: 2022 2022
Size: 4502
BglI GCCn_nnn'nGGC
Cuts at: 112 184 112
Size: 72 4430
BglII A'GATC_T
Cuts at: 2017 4054 2017
Size: 2037 2465
BlpI GC'TnA_GC
Cuts at: 1614 1614
Size: 4502
Bme1580I G_kGCm'C
Cuts at: 2127 2220 3276 2127
Size: 93 1056 3353
Fragments arranged by size:
3353 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1714 1795 2130 4274 1714
Size: 81 335 2144 1942
Fragments arranged by size:
2144 1942 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2869 2869
Size: 4502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 751 1115 464
Size: 287 364 3851
Fragments arranged by size:
3851 364 287
Bpu10I CC'TnA_GC
Cuts at: 194 1022 1355 1452 194
Size: 828 333 97 3244
Fragments arranged by size:
3244 828 333 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1678 2557 3049 3347 1678
Size: 879 492 298 2833
Fragments arranged by size:
2833 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4206 4206
Size: 4502
BsaAI yAC'GTr
Cuts at: 1855 1855
Size: 4502
BsaBI GATnn'nnATC
Cuts at: 6 2016 4053 6
Size: 2010 2037 455
Fragments arranged by size:
2037 2010 455
BsaHI Gr'CG_yC
Cuts at: 1933 2181 2883 4379 1933
Size: 248 702 1496 2056
Fragments arranged by size:
2056 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1691 2212 2890 3164 3311 3722 1691
Size: 521 678 274 147 411 2471
Fragments arranged by size:
2471 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 749 779 749
Size: 30 4472
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 473 473
Size: 4502
BseYI C'CCAG_C
Cuts at: 179 1927 2685 3262 3758 179
Size: 1748 758 577 496 923
Fragments arranged by size:
1748 923 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2044 2090 3298 104
Size: 1940 46 1208 1308
Fragments arranged by size:
1940 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1516 2294 2484 3276 1516
Size: 778 190 792 2742
Fragments arranged by size:
2742 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1516 2127 2220 2294 2484 2546 3276 1516
Size: 611 93 74 190 62 730 2742
Fragments arranged by size:
2742 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3722 3722
Size: 4502
BspHI T'CATG_A
Cuts at: 3678 3678
Size: 4502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1552 1766 2068 2449 1552
Size: 214 302 381 3605
Fragments arranged by size:
3605 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2794 2848 4332 4497 50
Size: 2744 54 1484 165 55
Fragments arranged by size:
2744 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2414 2414
Size: 4502
BsrFI r'CCGG_y
Cuts at: 164 2500 2681 2890 164
Size: 2336 181 209 1776
Fragments arranged by size:
2336 1776 209 181
BssSI C'ACGA_G
Cuts at: 1340 2773 3131 1340
Size: 1433 358 2711
Fragments arranged by size:
2711 1433 358
BstAPI GCAn_nnn'nTGC
Cuts at: 560 1879 560
Size: 1319 3183
BstBI TT'CG_AA
Cuts at: 2863 2863
Size: 4502
BstYI r'GATC_y
Cuts at: 1 1527 1994 2017 2352 2598 3610 3696 3708
Size: 1526 467 23 335 246 1012 86 12
Cuts at: 3708 4054 1
Size: 346 449
Fragments arranged by size:
1526 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 143 228 107
Size: 36 85 4381
Fragments arranged by size:
4381 85 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2452 2633 2796 2452
Size: 181 163 4158
Fragments arranged by size:
4158 181 163
BtsI GCAGTG_nn'
Cuts at: 1745 1745
Size: 4502
DraI TTT'AAA
Cuts at: 1501 1501
Size: 4502
DraIII CAC_nnn'GTG
Cuts at: 670 670
Size: 4502
DrdI GACnn_nn'nnGTC
Cuts at: 2208 3066 2208
Size: 858 3644
EaeI y'GGCC_r
Cuts at: 140 146 1094 2087 2261 2652 2679 4024 140
Size: 6 948 993 174 391 27 1345 618
Fragments arranged by size:
1345 993 948 618 391 174 27 6
EagI C'GGCC_G
Cuts at: 2087 2087
Size: 4502
EarI CTCTTCn'nnn_
Cuts at: 2525 2735 2525
Size: 210 4292
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2710 3020 3166 4312 2710
Size: 310 146 1146 2900
Fragments arranged by size:
2900 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1514 1514
Size: 4502
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 728 751 1115 1187 2327 2759 3506 3774
Size: 264 23 364 72 1140 432 747 268
Cuts at: 3774 464
Size: 1192
Fragments arranged by size:
1192 1140 747 432 364 268 264 72
23
EcoO109I rG'GnC_Cy
Cuts at: 643 1641 643
Size: 998 3504
EcoRI G'AATT_C
Cuts at: 1506 1506
Size: 4502
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 987 2521 987
Size: 1534 2968
FspI TGC'GCA
Cuts at: 2283 2283
Size: 4502
HaeII r_GCGC'y
Cuts at: 1936 2184 3206 4185 1936
Size: 248 1022 979 2253
Fragments arranged by size:
2253 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2344 2374 2376 2406 2344
Size: 30 2 30 4440
Fragments arranged by size:
4440 30 30 2
HincII GTy'rAC
Cuts at: 678 1233 1541 678
Size: 555 308 3639
Fragments arranged by size:
3639 555 308
HindIII A'AGCT_T
Cuts at: 353 832 1192 353
Size: 479 360 3663
Fragments arranged by size:
3663 479 360
KasI G'GCGC_C
Cuts at: 1932 2180 1932
Size: 248 4254
KpnI G_GTAC'C
Cuts at: 1522 1522
Size: 4502
MfeI C'AATT_G
Cuts at: 362 362
Size: 4502
MluI A'CGCG_T
Cuts at: 3935 3935
Size: 4502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3173 3357 3173
Size: 184 4318
MscI TGG'CCA
Cuts at: 148 1096 2263 148
Size: 948 1167 2387
Fragments arranged by size:
2387 1167 948
MslI CAynn'nnrTG
Cuts at: 286 671 1472 2491 2618 286
Size: 385 801 1019 127 2170
Fragments arranged by size:
2170 1019 801 385 127
MspA1I CmG'CkG
Cuts at: 183 1613 1927 2287 3300 3545 183
Size: 1430 314 360 1013 245 1140
Fragments arranged by size:
1430 1140 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2683 2683
Size: 4502
NarI GG'CG_CC
Cuts at: 1933 2181 1933
Size: 248 4254
NcoI C'CATG_G
Cuts at: 107 228 107
Size: 121 4381
NgoMIV G'CCGG_C
Cuts at: 2681 2681
Size: 4502
NspI r_CATG'y
Cuts at: 1471 1555 2055 2962 1471
Size: 84 500 907 3011
Fragments arranged by size:
3011 907 500 84
PciI A'CATG_T
Cuts at: 1467 2958 1467
Size: 1491 3011
PmeI GTTT'AAAC
Cuts at: 1501 1501
Size: 4502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 749 781 3641 3673 749
Size: 32 2860 32 1578
Fragments arranged by size:
2860 1578 32 32
PpuMI rG'GwC_Cy
Cuts at: 643 643
Size: 4502
PstI C_TGCA'G
Cuts at: 1549 2234 1549
Size: 685 3817
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4502
PvuII CAG'CTG
Cuts at: 1927 2287 1927
Size: 360 4142
RsrII CG'GwC_CG
Cuts at: 2697 2697
Size: 4502
SacI G_AGCT'C
Cuts at: 1516 1516
Size: 4502
SalI G'TCGA_C
Cuts at: 1539 1539
Size: 4502
SapI GCTCTTCn'nnn_
Cuts at: 2525 2735 2525
Size: 210 4292
SbfI CC_TGCA'GG
Cuts at: 1549 1549
Size: 4502
ScaI AGT'ACT
Cuts at: 4019 4019
Size: 4502
SfcI C'TryA_G
Cuts at: 33 979 1163 1545 2230 3223 3414 33
Size: 946 184 382 685 993 191 1121
Fragments arranged by size:
1121 993 946 685 382 191 184
SfoI GGC'GCC
Cuts at: 1934 2182 1934
Size: 248 4254
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
SmaI CCC'GGG
Cuts at: 1524 1524
Size: 4502
SmlI C'TyrA_G
Cuts at: 1657 2572 3064 3326 1657
Size: 915 492 262 2833
Fragments arranged by size:
2833 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4502
SphI G_CATG'C
Cuts at: 1555 2055 1555
Size: 500 4002
SspI AAT'ATT
Cuts at: 801 801
Size: 4502
StyI C'CwwG_G
Cuts at: 107 228 1636 1990 107
Size: 121 1408 354 2619
Fragments arranged by size:
2619 1408 354 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2085 2218 2677 2886 2085
Size: 133 459 209 3701
Fragments arranged by size:
3701 459 209 133
TatI w'GTAC_w
Cuts at: 240 4017 240
Size: 3777 725
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4019 4051 4019
Size: 32 4470
Tth111I GACn'n_nGTC
Cuts at: 2299 2299
Size: 4502
XbaI T'CTAG_A
Cuts at: 1533 1533
Size: 4502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 330 1997 330
Size: 1667 2835
XmaI C'CCGG_G
Cuts at: 1522 1522
Size: 4502
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BbsI BbvCI BcgI BfrBI BmgBI BmtI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI