Restriction map for
pF1KB5158
(Circular) (Six-base) MAPSORT of: pF1KB5158.seq Check: 3822 from: 1 to: 3863
pF1KB5158 3863 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 901 901
Size: 3863
Acc65I G'GTAC_C
Cuts at: 879 879
Size: 3863
AclI AA'CG_TT
Cuts at: 3703 3703
Size: 3863
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 1688 2120 2867 3135 352
Size: 1336 432 747 268 1080
Fragments arranged by size:
1336 1080 747 432 268
AflIII A'CryG_T
Cuts at: 729 1215 2319 3296 729
Size: 486 1104 977 1296
Fragments arranged by size:
1296 1104 977 486
AgeI A'CCGG_T
Cuts at: 2251 2251
Size: 3863
AlwNI CAG_nnn'CTG
Cuts at: 1168 2735 1168
Size: 1567 2296
ApaLI G'TGCA_C
Cuts at: 2633 2633
Size: 3863
ApoI r'AATT_y
Cuts at: 349 867 3216 3472 349
Size: 518 2349 256 740
Fragments arranged by size:
2349 740 518 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3863
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3863
AvaI C'yCGr_G
Cuts at: 883 883
Size: 3863
BamHI G'GATC_C
Cuts at: 888 888
Size: 3863
BanI G'GyrC_C
Cuts at: 879 1293 1541 1576 879
Size: 414 248 35 3166
Fragments arranged by size:
3166 414 248 35
BanII G_rGCy'C
Cuts at: 877 1907 877
Size: 1030 2833
BbeI G_GCGC'C
Cuts at: 1297 1545 1297
Size: 248 3615
BciVI GTATCCnnnnn_n'
Cuts at: 1761 2522 3863 1761
Size: 761 1341 1761
Fragments arranged by size:
1761 1341 761
BclI T'GATC_A
Cuts at: 1383 1383
Size: 3863
BfrBI ATG'CAT
Cuts at: 499 499
Size: 3863
BglII A'GATC_T
Cuts at: 1378 3415 1378
Size: 2037 1826
BlpI GC'TnA_GC
Cuts at: 140 237 975 140
Size: 97 738 3028
Fragments arranged by size:
3028 738 97
Bme1580I G_kGCm'C
Cuts at: 1488 1581 2637 1488
Size: 93 1056 2714
Fragments arranged by size:
2714 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 398 1075 1156 1491 3635 398
Size: 677 81 335 2144 626
Fragments arranged by size:
2144 677 626 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 174 459 2230 174
Size: 285 1771 1807
Fragments arranged by size:
1807 1771 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 310 166
Size: 144 3719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1039 1918 2410 2708 346
Size: 693 879 492 298 1501
Fragments arranged by size:
1501 879 693 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3567 3567
Size: 3863
BsaAI yAC'GTr
Cuts at: 1216 1216
Size: 3863
BsaBI GATnn'nnATC
Cuts at: 6 1377 3414 6
Size: 1371 2037 455
Fragments arranged by size:
2037 1371 455
BsaHI Gr'CG_yC
Cuts at: 1294 1542 2244 3740 1294
Size: 248 702 1496 1417
Fragments arranged by size:
1496 1417 702 248
BsaWI w'CCGG_w
Cuts at: 1052 1573 2251 2525 2672 3083 1052
Size: 521 678 274 147 411 1832
Fragments arranged by size:
1832 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 240 270 240
Size: 30 3833
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 132 163 132
Size: 31 3832
BseYI C'CCAG_C
Cuts at: 1288 2046 2623 3119 1288
Size: 758 577 496 2032
Fragments arranged by size:
2032 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1405 1451 2659 104
Size: 1301 46 1208 1308
Fragments arranged by size:
1308 1301 1208 46
BsiHKAI G_wGCw'C
Cuts at: 877 1655 1845 2637 877
Size: 778 190 792 2103
Fragments arranged by size:
2103 792 778 190
BsmI GAATG_Cn'
Cuts at: 403 403
Size: 3863
BsmBI CGTCTCn'nnnn_
Cuts at: 260 260
Size: 3863
Bsp1286I G_dGCh'C
Cuts at: 877 1488 1581 1655 1845 1907 2637 877
Size: 611 93 74 190 62 730 2103
Fragments arranged by size:
2103 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3083 3083
Size: 3863
BspHI T'CATG_A
Cuts at: 218 3039 218
Size: 2821 1042
BspMI ACCTGCnnnn'nnnn_
Cuts at: 913 1127 1429 1810 913
Size: 214 302 381 2966
Fragments arranged by size:
2966 381 302 214
BsrBI CCG'CTC
Cuts at: 50 213 2155 2209 3693 3858 50
Size: 163 1942 54 1484 165 55
Fragments arranged by size:
1942 1484 165 163 55 54
BsrDI GCAATG_nn'
Cuts at: 1775 1775
Size: 3863
BsrFI r'CCGG_y
Cuts at: 1861 2042 2251 1861
Size: 181 209 3473
Fragments arranged by size:
3473 209 181
BsrGI T'GTAC_A
Cuts at: 691 691
Size: 3863
BssSI C'ACGA_G
Cuts at: 2134 2492 2134
Size: 358 3505
BstAPI GCAn_nnn'nTGC
Cuts at: 1240 1240
Size: 3863
BstBI TT'CG_AA
Cuts at: 2224 2224
Size: 3863
BstYI r'GATC_y
Cuts at: 1 371 888 1355 1378 1713 1959 2971 3057
Size: 370 517 467 23 335 246 1012 86
Cuts at: 3057 3069 3415 1
Size: 12 346 449
Fragments arranged by size:
1012 517 467 449 370 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1813 1994 2157 1813
Size: 181 163 3519
Fragments arranged by size:
3519 181 163
BtsI GCAGTG_nn'
Cuts at: 1106 1106
Size: 3863
DraI TTT'AAA
Cuts at: 862 862
Size: 3863
DrdI GACnn_nn'nnGTC
Cuts at: 1569 2427 1569
Size: 858 3005
EaeI y'GGCC_r
Cuts at: 296 1448 1622 2013 2040 3385 296
Size: 1152 174 391 27 1345 774
Fragments arranged by size:
1345 1152 774 391 174 27
EagI C'GGCC_G
Cuts at: 1448 1448
Size: 3863
EarI CTCTTCn'nnn_
Cuts at: 1886 2096 1886
Size: 210 3653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2071 2381 2527 3673 2071
Size: 310 146 1146 2261
Fragments arranged by size:
2261 1146 310 146
EcoICRI GAG'CTC
Cuts at: 875 875
Size: 3863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 310 352 1688 2120 2867 3135 166
Size: 144 42 1336 432 747 268 894
Fragments arranged by size:
1336 894 747 432 268 144 42
EcoO109I rG'GnC_Cy
Cuts at: 1002 1002
Size: 3863
EcoRI G'AATT_C
Cuts at: 867 867
Size: 3863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1882 1882
Size: 3863
FspI TGC'GCA
Cuts at: 1644 1644
Size: 3863
HaeII r_GCGC'y
Cuts at: 1297 1545 2567 3546 1297
Size: 248 1022 979 1614
Fragments arranged by size:
1614 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1705 1735 1737 1767 1705
Size: 30 2 30 3801
Fragments arranged by size:
3801 30 30 2
HincII GTy'rAC
Cuts at: 902 902
Size: 3863
KasI G'GCGC_C
Cuts at: 1293 1541 1293
Size: 248 3615
KpnI G_GTAC'C
Cuts at: 883 883
Size: 3863
MfeI C'AATT_G
Cuts at: 591 591
Size: 3863
MluI A'CGCG_T
Cuts at: 3296 3296
Size: 3863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2534 2718 2534
Size: 184 3679
MscI TGG'CCA
Cuts at: 298 1624 298
Size: 1326 2537
MslI CAynn'nnrTG
Cuts at: 112 1852 1979 112
Size: 1740 127 1996
Fragments arranged by size:
1996 1740 127
MspA1I CmG'CkG
Cuts at: 144 241 974 1288 1648 2661 2906 144
Size: 97 733 314 360 1013 245 1101
Fragments arranged by size:
1101 1013 733 360 314 245 97
NaeI GCC'GGC
Cuts at: 2044 2044
Size: 3863
NarI GG'CG_CC
Cuts at: 1294 1542 1294
Size: 248 3615
NdeI CA'TA_TG
Cuts at: 477 477
Size: 3863
NgoMIV G'CCGG_C
Cuts at: 2042 2042
Size: 3863
NsiI A_TGCA'T
Cuts at: 501 501
Size: 3863
NspI r_CATG'y
Cuts at: 916 1416 2323 916
Size: 500 907 2456
Fragments arranged by size:
2456 907 500
PciI A'CATG_T
Cuts at: 2319 2319
Size: 3863
PmeI GTTT'AAAC
Cuts at: 862 862
Size: 3863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3002 3034 3002
Size: 32 3831
PstI C_TGCA'G
Cuts at: 910 1595 910
Size: 685 3178
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3863
PvuII CAG'CTG
Cuts at: 144 241 1288 1648 144
Size: 97 1047 360 2359
Fragments arranged by size:
2359 1047 360 97
RsrII CG'GwC_CG
Cuts at: 2058 2058
Size: 3863
SacI G_AGCT'C
Cuts at: 877 877
Size: 3863
SalI G'TCGA_C
Cuts at: 900 900
Size: 3863
SapI GCTCTTCn'nnn_
Cuts at: 1886 2096 1886
Size: 210 3653
SbfI CC_TGCA'GG
Cuts at: 910 910
Size: 3863
ScaI AGT'ACT
Cuts at: 3380 3380
Size: 3863
SfcI C'TryA_G
Cuts at: 33 161 906 1591 2584 2775 33
Size: 128 745 685 993 191 1121
Fragments arranged by size:
1121 993 745 685 191 128
SfoI GGC'GCC
Cuts at: 1295 1543 1295
Size: 248 3615
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3863
SmaI CCC'GGG
Cuts at: 885 885
Size: 3863
SmlI C'TyrA_G
Cuts at: 325 1018 1933 2425 2687 325
Size: 693 915 492 262 1501
Fragments arranged by size:
1501 915 693 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3863
SphI G_CATG'C
Cuts at: 916 1416 916
Size: 500 3363
StyI C'CwwG_G
Cuts at: 997 1351 997
Size: 354 3509
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1446 1579 2038 2247 1446
Size: 133 459 209 3062
Fragments arranged by size:
3062 459 209 133
TatI w'GTAC_w
Cuts at: 576 691 3378 576
Size: 115 2687 1061
Fragments arranged by size:
2687 1061 115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3380 3412 3380
Size: 32 3831
Tth111I GACn'n_nGTC
Cuts at: 1660 1660
Size: 3863
XbaI T'CTAG_A
Cuts at: 894 894
Size: 3863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1358 1358
Size: 3863
XmaI C'CCGG_G
Cuts at: 883 883
Size: 3863
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NcoI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI