Restriction map for
pF1KB5126
(Circular) (Six-base) MAPSORT of: pF1KB5126.seq Check: 5984 from: 1 to: 4139
pF1KB5126 4139 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 567 567
Size: 4139
AccI GT'mk_AC
Cuts at: 493 1177 493
Size: 684 3455
Acc65I G'GTAC_C
Cuts at: 212 1155 212
Size: 943 3196
AclI AA'CG_TT
Cuts at: 3979 3979
Size: 4139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1964 2396 3143 3411 1964
Size: 432 747 268 2692
Fragments arranged by size:
2692 747 432 268
AflIII A'CryG_T
Cuts at: 1491 2595 3572 1491
Size: 1104 977 2058
Fragments arranged by size:
2058 1104 977
AgeI A'CCGG_T
Cuts at: 215 2527 215
Size: 2312 1827
AhdI GACnn_n'nnGTC
Cuts at: 489 489
Size: 4139
AlwNI CAG_nnn'CTG
Cuts at: 412 1111 1444 3011 412
Size: 699 333 1567 1540
Fragments arranged by size:
1567 1540 699 333
ApaLI G'TGCA_C
Cuts at: 2909 2909
Size: 4139
ApoI r'AATT_y
Cuts at: 680 1143 3492 3748 680
Size: 463 2349 256 1071
Fragments arranged by size:
2349 1071 463 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4139
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
AvaI C'yCGr_G
Cuts at: 149 238 1159 149
Size: 89 921 3129
Fragments arranged by size:
3129 921 89
BamHI G'GATC_C
Cuts at: 1164 1164
Size: 4139
BanI G'GyrC_C
Cuts at: 212 1155 1569 1817 1852 212
Size: 943 414 248 35 2499
Fragments arranged by size:
2499 943 414 248 35
BanII G_rGCy'C
Cuts at: 514 603 859 1153 2183 514
Size: 89 256 294 1030 2470
Fragments arranged by size:
2470 1030 294 256 89
BbeI G_GCGC'C
Cuts at: 1573 1821 1573
Size: 248 3891
BbsI GAAGACnn'nnnn_
Cuts at: 941 941
Size: 4139
BbvCI CC'TCA_GC
Cuts at: 773 773
Size: 4139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 391 425 90
Size: 34 267 34 3804
Fragments arranged by size:
3804 267 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 687 2037 2798 4139 687
Size: 1350 761 1341 687
Fragments arranged by size:
1350 1341 761 687
BclI T'GATC_A
Cuts at: 1659 1659
Size: 4139
BglII A'GATC_T
Cuts at: 1654 3691 1654
Size: 2037 2102
BlpI GC'TnA_GC
Cuts at: 572 858 1251 572
Size: 286 393 3460
Fragments arranged by size:
3460 393 286
Bme1580I G_kGCm'C
Cuts at: 140 1091 1764 1857 2913 140
Size: 951 673 93 1056 1366
Fragments arranged by size:
1366 1056 951 673 93
BmrI ACTGGGnnnn_n'
Cuts at: 867 893 1351 1432 1767 3911 867
Size: 26 458 81 335 2144 1095
Fragments arranged by size:
2144 1095 458 335 81 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 4139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 371 231
Size: 140 3999
Bpu10I CC'TnA_GC
Cuts at: 611 773 853 611
Size: 162 80 3897
Fragments arranged by size:
3897 162 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 945 1315 2194 2686 2984 528
Size: 417 370 879 492 298 1683
Fragments arranged by size:
1683 879 492 417 370 298
BsaI GGTCTCn'nnnn_
Cuts at: 3843 3843
Size: 4139
BsaAI yAC'GTr
Cuts at: 1492 1492
Size: 4139
BsaBI GATnn'nnATC
Cuts at: 6 1653 3690 6
Size: 1647 2037 455
Fragments arranged by size:
2037 1647 455
BsaHI Gr'CG_yC
Cuts at: 1570 1818 2520 4016 1570
Size: 248 702 1496 1693
Fragments arranged by size:
1693 1496 702 248
BsaWI w'CCGG_w
Cuts at: 215 1328 1849 2527 2801 2948 3359 215
Size: 1113 521 678 274 147 411 995
Fragments arranged by size:
1113 995 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 626 226
Size: 400 3739
BseYI C'CCAG_C
Cuts at: 266 1058 1104 1564 2322 2899 3395 266
Size: 792 46 460 758 577 496 1010
Fragments arranged by size:
1010 792 758 577 496 460 46
BsiEI CG_ry'CG
Cuts at: 104 1681 1727 2935 104
Size: 1577 46 1208 1308
Fragments arranged by size:
1577 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 514 603 859 1153 1931 2121 2913 514
Size: 89 256 294 778 190 792 1740
Fragments arranged by size:
1740 792 778 294 256 190 89
Bsp1286I G_dGCh'C
Cuts at: 140 514 603 859 1091 1153 1764 1857 1931
Size: 374 89 256 232 62 611 93 74
Cuts at: 1931 2121 2183 2913 140
Size: 190 62 730 1366
Fragments arranged by size:
1366 730 611 374 256 232 190 93
89 74 62 62
BspEI T'CCGG_A
Cuts at: 3359 3359
Size: 4139
BspHI T'CATG_A
Cuts at: 3315 3315
Size: 4139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 567 1125 1189 1403 1705 2086 567
Size: 558 64 214 302 381 2620
Fragments arranged by size:
2620 558 381 302 214 64
BsrBI CCG'CTC
Cuts at: 50 1029 2431 2485 3969 4134 50
Size: 979 1402 54 1484 165 55
Fragments arranged by size:
1484 1402 979 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2051 2051
Size: 4139
BsrFI r'CCGG_y
Cuts at: 215 271 2137 2318 2527 215
Size: 56 1866 181 209 1827
Fragments arranged by size:
1866 1827 209 181 56
BsrGI T'GTAC_A
Cuts at: 864 864
Size: 4139
BssSI C'ACGA_G
Cuts at: 2410 2768 2410
Size: 358 3781
BstAPI GCAn_nnn'nTGC
Cuts at: 464 1516 464
Size: 1052 3087
BstBI TT'CG_AA
Cuts at: 2500 2500
Size: 4139
BstYI r'GATC_y
Cuts at: 1 1164 1631 1654 1989 2235 3247 3333 3345
Size: 1163 467 23 335 246 1012 86 12
Cuts at: 3345 3691 1
Size: 346 449
Fragments arranged by size:
1163 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2089 2270 2433 2089
Size: 181 163 3795
Fragments arranged by size:
3795 181 163
BtsI GCAGTG_nn'
Cuts at: 738 1382 738
Size: 644 3495
DraI TTT'AAA
Cuts at: 1138 1138
Size: 4139
DraIII CAC_nnn'GTG
Cuts at: 907 1086 907
Size: 179 3960
DrdI GACnn_nn'nnGTC
Cuts at: 1069 1845 2703 1069
Size: 776 858 2505
Fragments arranged by size:
2505 858 776
EaeI y'GGCC_r
Cuts at: 1724 1898 2289 2316 3661 1724
Size: 174 391 27 1345 2202
Fragments arranged by size:
2202 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1724 1724
Size: 4139
EarI CTCTTCn'nnn_
Cuts at: 2162 2372 2162
Size: 210 3929
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 318 2347 2657 2803 3949 318
Size: 2029 310 146 1146 508
Fragments arranged by size:
2029 1146 508 310 146
EcoICRI GAG'CTC
Cuts at: 512 601 857 1151 512
Size: 89 256 294 3500
Fragments arranged by size:
3500 294 256 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 371 1964 2396 3143 3411 231
Size: 140 1593 432 747 268 959
Fragments arranged by size:
1593 959 747 432 268 140
EcoO109I rG'GnC_Cy
Cuts at: 242 294 564 1278 242
Size: 52 270 714 3103
Fragments arranged by size:
3103 714 270 52
EcoRI G'AATT_C
Cuts at: 1143 1143
Size: 4139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 4139
FspI TGC'GCA
Cuts at: 1920 1920
Size: 4139
HaeII r_GCGC'y
Cuts at: 232 264 1573 1821 2843 3822 232
Size: 32 1309 248 1022 979 549
Fragments arranged by size:
1309 1022 979 549 248 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 475 507 1981 2011 2013 2043 475
Size: 32 1474 30 2 30 2571
Fragments arranged by size:
2571 1474 32 30 30 2
HincII GTy'rAC
Cuts at: 1178 1178
Size: 4139
KasI G'GCGC_C
Cuts at: 1569 1817 1569
Size: 248 3891
KpnI G_GTAC'C
Cuts at: 216 1159 216
Size: 943 3196
MluI A'CGCG_T
Cuts at: 3572 3572
Size: 4139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 179 2810 2994 179
Size: 2631 184 1324
Fragments arranged by size:
2631 1324 184
MscI TGG'CCA
Cuts at: 1900 1900
Size: 4139
MslI CAynn'nnrTG
Cuts at: 2128 2255 2128
Size: 127 4012
MspA1I CmG'CkG
Cuts at: 701 862 1250 1564 1924 2937 3182 701
Size: 161 388 314 360 1013 245 1658
Fragments arranged by size:
1658 1013 388 360 314 245 161
NaeI GCC'GGC
Cuts at: 273 2320 273
Size: 2047 2092
NarI GG'CG_CC
Cuts at: 1570 1818 1570
Size: 248 3891
NgoMIV G'CCGG_C
Cuts at: 271 2318 271
Size: 2047 2092
NspI r_CATG'y
Cuts at: 1192 1692 2599 1192
Size: 500 907 2732
Fragments arranged by size:
2732 907 500
PciI A'CATG_T
Cuts at: 2595 2595
Size: 4139
PflMI CCAn_nnn'nTGG
Cuts at: 1111 1111
Size: 4139
PmeI GTTT'AAAC
Cuts at: 1138 1138
Size: 4139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 4107
PpuMI rG'GwC_Cy
Cuts at: 294 564 294
Size: 270 3869
PstI C_TGCA'G
Cuts at: 733 1186 1871 733
Size: 453 685 3001
Fragments arranged by size:
3001 685 453
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4139
PvuII CAG'CTG
Cuts at: 862 1564 1924 862
Size: 702 360 3077
Fragments arranged by size:
3077 702 360
RsrII CG'GwC_CG
Cuts at: 218 2334 218
Size: 2116 2023
SacI G_AGCT'C
Cuts at: 514 603 859 1153 514
Size: 89 256 294 3500
Fragments arranged by size:
3500 294 256 89
SalI G'TCGA_C
Cuts at: 1176 1176
Size: 4139
SapI GCTCTTCn'nnn_
Cuts at: 2162 2372 2162
Size: 210 3929
SbfI CC_TGCA'GG
Cuts at: 1186 1186
Size: 4139
ScaI AGT'ACT
Cuts at: 3656 3656
Size: 4139
SfcI C'TryA_G
Cuts at: 33 494 729 1182 1867 2860 3051 33
Size: 461 235 453 685 993 191 1121
Fragments arranged by size:
1121 993 685 461 453 235 191
SfoI GGC'GCC
Cuts at: 1571 1819 1571
Size: 248 3891
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
SmaI CCC'GGG
Cuts at: 151 1161 151
Size: 1010 3129
SmlI C'TyrA_G
Cuts at: 507 960 1294 2209 2701 2963 507
Size: 453 334 915 492 262 1683
Fragments arranged by size:
1683 915 492 453 334 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4139
SphI G_CATG'C
Cuts at: 1192 1692 1192
Size: 500 3639
StyI C'CwwG_G
Cuts at: 1273 1627 1273
Size: 354 3785
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1722 1855 2314 2523 1722
Size: 133 459 209 3338
Fragments arranged by size:
3338 459 209 133
TatI w'GTAC_w
Cuts at: 838 864 3654 838
Size: 26 2790 1323
Fragments arranged by size:
2790 1323 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3656 3688 3656
Size: 32 4107
Tth111I GACn'n_nGTC
Cuts at: 1020 1936 1020
Size: 916 3223
XbaI T'CTAG_A
Cuts at: 1170 1170
Size: 4139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 346 880 1634 346
Size: 534 754 2851
Fragments arranged by size:
2851 754 534
XmaI C'CCGG_G
Cuts at: 149 1159 149
Size: 1010 3129
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsaXI BsgI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI