Restriction map for
pF1KB5055
(Circular) (Six-base) MAPSORT of: pF1KB5055.seq Check: 570 from: 1 to: 4655
pF1KB5055 4655 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1045 1045
Size: 4655
AccI GT'mk_AC
Cuts at: 1693 1693
Size: 4655
Acc65I G'GTAC_C
Cuts at: 1671 1671
Size: 4655
AclI AA'CG_TT
Cuts at: 4495 4495
Size: 4655
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 808 2480 2912 3659 3927 709
Size: 99 1672 432 747 268 1437
Fragments arranged by size:
1672 1437 747 432 268 99
AflII C'TTAA_G
Cuts at: 289 1570 289
Size: 1281 3374
AflIII A'CryG_T
Cuts at: 2007 3111 4088 2007
Size: 1104 977 2574
Fragments arranged by size:
2574 1104 977
AgeI A'CCGG_T
Cuts at: 3043 3043
Size: 4655
AhdI GACnn_n'nnGTC
Cuts at: 1458 1458
Size: 4655
AlwNI CAG_nnn'CTG
Cuts at: 334 460 840 1960 3527 334
Size: 126 380 1120 1567 1462
Fragments arranged by size:
1567 1462 1120 380 126
ApaLI G'TGCA_C
Cuts at: 3425 3425
Size: 4655
ApoI r'AATT_y
Cuts at: 400 704 942 1471 1659 4008 4264 400
Size: 304 238 529 188 2349 256 791
Fragments arranged by size:
2349 791 529 304 256 238 188
AseI AT'TA_AT
Cuts at: 20 20
Size: 4655
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
AvaI C'yCGr_G
Cuts at: 1675 1675
Size: 4655
BamHI G'GATC_C
Cuts at: 1322 1680 1322
Size: 358 4297
BanI G'GyrC_C
Cuts at: 1522 1671 2085 2333 2368 1522
Size: 149 414 248 35 3809
Fragments arranged by size:
3809 414 248 149 35
BanII G_rGCy'C
Cuts at: 1270 1669 2699 1270
Size: 399 1030 3226
Fragments arranged by size:
3226 1030 399
BbeI G_GCGC'C
Cuts at: 2089 2337 2089
Size: 248 4407
BbsI GAAGACnn'nnnn_
Cuts at: 662 662
Size: 4655
BbvCI CC'TCA_GC
Cuts at: 469 1378 1593 469
Size: 909 215 3531
Fragments arranged by size:
3531 909 215
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 223 257 223
Size: 34 4621
BciVI GTATCCnnnnn_n'
Cuts at: 541 2553 3314 4655 541
Size: 2012 761 1341 541
Fragments arranged by size:
2012 1341 761 541
BclI T'GATC_A
Cuts at: 965 2175 965
Size: 1210 3445
BglI GCCn_nnn'nGGC
Cuts at: 1246 1246
Size: 4655
BglII A'GATC_T
Cuts at: 2170 4207 2170
Size: 2037 2618
BlpI GC'TnA_GC
Cuts at: 1767 1767
Size: 4655
Bme1580I G_kGCm'C
Cuts at: 136 2280 2373 3429 136
Size: 2144 93 1056 1362
Fragments arranged by size:
2144 1362 1056 93
BmgBI CAC'GTC
Cuts at: 137 137
Size: 4655
BmrI ACTGGGnnnn_n'
Cuts at: 1867 1948 2283 4427 1867
Size: 81 335 2144 2095
Fragments arranged by size:
2144 2095 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 211 3022 211
Size: 2811 1844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 570 570
Size: 4655
Bpu10I CC'TnA_GC
Cuts at: 469 1378 1593 469
Size: 909 215 3531
Fragments arranged by size:
3531 909 215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1831 2710 3202 3500 1831
Size: 879 492 298 2986
Fragments arranged by size:
2986 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4359 4359
Size: 4655
BsaAI yAC'GTr
Cuts at: 144 2008 144
Size: 1864 2791
BsaBI GATnn'nnATC
Cuts at: 6 2169 4206 6
Size: 2163 2037 455
Fragments arranged by size:
2163 2037 455
BsaHI Gr'CG_yC
Cuts at: 1613 2086 2334 3036 4532 1613
Size: 473 248 702 1496 1736
Fragments arranged by size:
1736 1496 702 473 248
BsaWI w'CCGG_w
Cuts at: 1185 1844 2365 3043 3317 3464 3875 1185
Size: 659 521 678 274 147 411 1965
Fragments arranged by size:
1965 678 659 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1001 1031 1558 1588 1001
Size: 30 527 30 4068
Fragments arranged by size:
4068 527 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 573 625 573
Size: 52 4603
BseYI C'CCAG_C
Cuts at: 334 2080 2838 3415 3911 334
Size: 1746 758 577 496 1078
Fragments arranged by size:
1746 1078 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1468 1468
Size: 4655
BsiEI CG_ry'CG
Cuts at: 104 2197 2243 3451 104
Size: 2093 46 1208 1308
Fragments arranged by size:
2093 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1669 2447 2637 3429 1669
Size: 778 190 792 2895
Fragments arranged by size:
2895 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 182 182
Size: 4655
Bsp1286I G_dGCh'C
Cuts at: 136 1270 1669 2280 2373 2447 2637 2699 3429
Size: 1134 399 611 93 74 190 62 730
Cuts at: 3429 136
Size: 1362
Fragments arranged by size:
1362 1134 730 611 399 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3875 3875
Size: 4655
BspHI T'CATG_A
Cuts at: 864 1259 3831 864
Size: 395 2572 1688
Fragments arranged by size:
2572 1688 395
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1045 1705 1919 2221 2602 1045
Size: 660 214 302 381 3098
Fragments arranged by size:
3098 660 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2947 3001 4485 4650 50
Size: 2897 54 1484 165 55
Fragments arranged by size:
2897 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 356 770 1020 1190 2567 356
Size: 414 250 170 1377 2444
Fragments arranged by size:
2444 1377 414 250 170
BsrFI r'CCGG_y
Cuts at: 2653 2834 3043 2653
Size: 181 209 4265
Fragments arranged by size:
4265 209 181
BsrGI T'GTAC_A
Cuts at: 391 1148 391
Size: 757 3898
BssSI C'ACGA_G
Cuts at: 2926 3284 2926
Size: 358 4297
BstAPI GCAn_nnn'nTGC
Cuts at: 1113 2032 1113
Size: 919 3736
BstBI TT'CG_AA
Cuts at: 3016 3016
Size: 4655
BstXI CCAn_nnnn'nTGG
Cuts at: 351 1006 1519 351
Size: 655 513 3487
Fragments arranged by size:
3487 655 513
BstYI r'GATC_y
Cuts at: 1 1322 1680 2147 2170 2505 2751 3763 3849
Size: 1321 358 467 23 335 246 1012 86
Cuts at: 3849 3861 4207 1
Size: 12 346 449
Fragments arranged by size:
1321 1012 467 449 358 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 314 1364 107
Size: 207 1050 3398
Fragments arranged by size:
3398 1050 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2605 2786 2949 2605
Size: 181 163 4311
Fragments arranged by size:
4311 181 163
BtsI GCAGTG_nn'
Cuts at: 239 1022 1151 1898 239
Size: 783 129 747 2996
Fragments arranged by size:
2996 783 747 129
DraI TTT'AAA
Cuts at: 1654 1654
Size: 4655
DrdI GACnn_nn'nnGTC
Cuts at: 463 2361 3219 463
Size: 1898 858 1899
Fragments arranged by size:
1899 1898 858
EaeI y'GGCC_r
Cuts at: 110 341 764 2240 2414 2805 2832 4177 110
Size: 231 423 1476 174 391 27 1345 588
Fragments arranged by size:
1476 1345 588 423 391 231 174 27
EagI C'GGCC_G
Cuts at: 2240 2240
Size: 4655
EarI CTCTTCn'nnn_
Cuts at: 181 629 936 1576 2678 2888 181
Size: 448 307 640 1102 210 1948
Fragments arranged by size:
1948 1102 640 448 307 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2863 3173 3319 4465 2863
Size: 310 146 1146 3053
Fragments arranged by size:
3053 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1667 1667
Size: 4655
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 570 709 808 2480 2912 3659 3927 570
Size: 139 99 1672 432 747 268 1298
Fragments arranged by size:
1672 1298 747 432 268 139 99
EcoO109I rG'GnC_Cy
Cuts at: 1247 1293 1794 1247
Size: 46 501 4108
Fragments arranged by size:
4108 501 46
EcoRI G'AATT_C
Cuts at: 400 1659 400
Size: 1259 3396
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 4655
FspI TGC'GCA
Cuts at: 2436 2436
Size: 4655
HaeII r_GCGC'y
Cuts at: 358 1176 2089 2337 3359 4338 358
Size: 818 913 248 1022 979 675
Fragments arranged by size:
1022 979 913 818 675 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 252 284 1254 1286 1444 1476 2497 2527 2529
Size: 32 970 32 158 32 1021 30 2
Cuts at: 2529 2559 252
Size: 30 2348
Fragments arranged by size:
2348 1021 970 158 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1694 1694
Size: 4655
KasI G'GCGC_C
Cuts at: 2085 2333 2085
Size: 248 4407
KpnI G_GTAC'C
Cuts at: 1675 1675
Size: 4655
MluI A'CGCG_T
Cuts at: 4088 4088
Size: 4655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1524 3326 3510 1524
Size: 1802 184 2669
Fragments arranged by size:
2669 1802 184
MscI TGG'CCA
Cuts at: 343 766 2416 343
Size: 423 1650 2582
Fragments arranged by size:
2582 1650 423
MslI CAynn'nnrTG
Cuts at: 349 869 1258 1631 2644 2771 349
Size: 520 389 373 1013 127 2233
Fragments arranged by size:
2233 1013 520 389 373 127
MspA1I CmG'CkG
Cuts at: 837 1766 2080 2440 3453 3698 837
Size: 929 314 360 1013 245 1794
Fragments arranged by size:
1794 1013 929 360 314 245
NaeI GCC'GGC
Cuts at: 2836 2836
Size: 4655
NarI GG'CG_CC
Cuts at: 2086 2334 2086
Size: 248 4407
NcoI C'CATG_G
Cuts at: 107 314 1364 107
Size: 207 1050 3398
Fragments arranged by size:
3398 1050 207
NgoMIV G'CCGG_C
Cuts at: 2834 2834
Size: 4655
NspI r_CATG'y
Cuts at: 1708 2208 3115 1708
Size: 500 907 3248
Fragments arranged by size:
3248 907 500
PasI CC'CwG_GG
Cuts at: 223 936 1428 223
Size: 713 492 3450
Fragments arranged by size:
3450 713 492
PciI A'CATG_T
Cuts at: 3111 3111
Size: 4655
PflMI CCAn_nnn'nTGG
Cuts at: 1215 1215
Size: 4655
PmeI GTTT'AAAC
Cuts at: 1654 1654
Size: 4655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 3794
Size: 32 4623
PshAI GACnn'nnGTC
Cuts at: 1540 1540
Size: 4655
PstI C_TGCA'G
Cuts at: 246 678 713 1702 2387 246
Size: 432 35 989 685 2514
Fragments arranged by size:
2514 989 685 432 35
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4655
PvuII CAG'CTG
Cuts at: 837 2080 2440 837
Size: 1243 360 3052
Fragments arranged by size:
3052 1243 360
RsrII CG'GwC_CG
Cuts at: 2850 2850
Size: 4655
SacI G_AGCT'C
Cuts at: 1669 1669
Size: 4655
SalI G'TCGA_C
Cuts at: 1692 1692
Size: 4655
SapI GCTCTTCn'nnn_
Cuts at: 181 2678 2888 181
Size: 2497 210 1948
Fragments arranged by size:
2497 1948 210
SbfI CC_TGCA'GG
Cuts at: 678 1702 678
Size: 1024 3631
ScaI AGT'ACT
Cuts at: 4172 4172
Size: 4655
SexAI A'CCwGG_T
Cuts at: 248 248
Size: 4655
SfcI C'TryA_G
Cuts at: 33 242 674 709 1698 2383 3376 3567 33
Size: 209 432 35 989 685 993 191 1121
Fragments arranged by size:
1121 993 989 685 432 209 191 35
SfiI GGCCn_nnn'nGGCC
Cuts at: 1246 1246
Size: 4655
SfoI GGC'GCC
Cuts at: 2087 2335 2087
Size: 248 4407
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
SmaI CCC'GGG
Cuts at: 1677 1677
Size: 4655
SmlI C'TyrA_G
Cuts at: 289 1570 1810 2725 3217 3479 289
Size: 1281 240 915 492 262 1465
Fragments arranged by size:
1465 1281 915 492 262 240
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4655
SphI G_CATG'C
Cuts at: 1708 2208 1708
Size: 500 4155
StyI C'CwwG_G
Cuts at: 107 314 476 506 1235 1242 1364 1789 2143
Size: 207 162 30 729 7 122 425 354
Cuts at: 2143 107
Size: 2619
Fragments arranged by size:
2619 729 425 354 207 162 122 30
7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2238 2371 2830 3039 2238
Size: 133 459 209 3854
Fragments arranged by size:
3854 459 209 133
TatI w'GTAC_w
Cuts at: 391 971 1148 1556 4170 391
Size: 580 177 408 2614 876
Fragments arranged by size:
2614 876 580 408 177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 386 418 1001 1033 4172 4204 386
Size: 32 583 32 3139 32 837
Fragments arranged by size:
3139 837 583 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 322 2452 322
Size: 2130 2525
XbaI T'CTAG_A
Cuts at: 1686 1686
Size: 4655
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1260 1435 1606 2150 1260
Size: 175 171 544 3765
Fragments arranged by size:
3765 544 175 171
XmaI C'CCGG_G
Cuts at: 1675 1675
Size: 4655
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AleI AlfI AloI ApaI AscI AvrII
BaeI BfrBI BmtI BsiWI BsmI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI
SacII SanDI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI