Restriction map for
pF1KB5045
(Circular) (Six-base) MAPSORT of: pF1KB5045.seq Check: 8234 from: 1 to: 5072
pF1KB5045 5072 bp
With 182 enzymes: SgfI *
February 15, 2010 17:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1087 1605 1087
Size: 518 4554
AccI GT'mk_AC
Cuts at: 173 2110 173
Size: 1937 3135
Acc65I G'GTAC_C
Cuts at: 341 977 2088 341
Size: 636 1111 3325
Fragments arranged by size:
3325 1111 636
AclI AA'CG_TT
Cuts at: 4912 4912
Size: 5072
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2897 3329 4076 4344 2897
Size: 432 747 268 3625
Fragments arranged by size:
3625 747 432 268
AflII C'TTAA_G
Cuts at: 1222 1222
Size: 5072
AflIII A'CryG_T
Cuts at: 2424 3528 4505 2424
Size: 1104 977 2991
Fragments arranged by size:
2991 1104 977
AgeI A'CCGG_T
Cuts at: 3460 3460
Size: 5072
AleI CACnn'nnGTG
Cuts at: 1366 1366
Size: 5072
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 232 264 232
Size: 32 5040
AlwNI CAG_nnn'CTG
Cuts at: 1363 2377 3944 1363
Size: 1014 1567 2491
Fragments arranged by size:
2491 1567 1014
ApaI G_GGCC'C
Cuts at: 236 1956 236
Size: 1720 3352
ApaLI G'TGCA_C
Cuts at: 133 288 3842 133
Size: 155 3554 1363
Fragments arranged by size:
3554 1363 155
ApoI r'AATT_y
Cuts at: 2076 4425 4681 2076
Size: 2349 256 2467
Fragments arranged by size:
2467 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5072
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5072
AvaI C'yCGr_G
Cuts at: 1184 2092 1184
Size: 908 4164
BamHI G'GATC_C
Cuts at: 2097 2097
Size: 5072
BanI G'GyrC_C
Cuts at: 341 615 977 1327 1766 2088 2502 2750 2785
Size: 274 362 350 439 322 414 248 35
Cuts at: 2785 341
Size: 2628
Fragments arranged by size:
2628 439 414 362 350 322 274 248
35
BanII G_rGCy'C
Cuts at: 236 563 650 835 1956 2086 3116 236
Size: 327 87 185 1121 130 1030 2192
Fragments arranged by size:
2192 1121 1030 327 185 130 87
BbeI G_GCGC'C
Cuts at: 2506 2754 2506
Size: 248 4824
BbsI GAAGACnn'nnnn_
Cuts at: 1684 1737 1832 1684
Size: 53 95 4924
Fragments arranged by size:
4924 95 53
BbvCI CC'TCA_GC
Cuts at: 225 973 225
Size: 748 4324
BciVI GTATCCnnnnn_n'
Cuts at: 2970 3731 5072 2970
Size: 761 1341 2970
Fragments arranged by size:
2970 1341 761
BclI T'GATC_A
Cuts at: 2592 2592
Size: 5072
BfrBI ATG'CAT
Cuts at: 1379 1379
Size: 5072
BglII A'GATC_T
Cuts at: 1496 2587 4624 1496
Size: 1091 2037 1944
Fragments arranged by size:
2037 1944 1091
BlpI GC'TnA_GC
Cuts at: 1282 1439 2184 1282
Size: 157 745 4170
Fragments arranged by size:
4170 745 157
Bme1580I G_kGCm'C
Cuts at: 137 236 292 620 1610 1956 2697 2790 3846
Size: 99 56 328 990 346 741 93 1056
Cuts at: 3846 137
Size: 1363
Fragments arranged by size:
1363 1056 990 741 346 328 99 93
56
BmgBI CAC'GTC
Cuts at: 1544 1544
Size: 5072
BmrI ACTGGGnnnn_n'
Cuts at: 1373 2284 2365 2700 4844 1373
Size: 911 81 335 2144 1601
Fragments arranged by size:
2144 1601 911 335 81
BmtI G_CTAG'C
Cuts at: 1042 1726 1042
Size: 684 4388
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 684 3439 684
Size: 2755 2317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 511 679 787 796 430
Size: 81 168 108 9 4706
Fragments arranged by size:
4706 168 108 81 9
Bpu10I CC'TnA_GC
Cuts at: 225 829 973 225
Size: 604 144 4324
Fragments arranged by size:
4324 604 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 685 823 2248 3127 3619 3917 634
Size: 51 138 1425 879 492 298 1789
Fragments arranged by size:
1789 1425 879 492 298 138 51
BsaI GGTCTCn'nnnn_
Cuts at: 1181 1480 4776 1181
Size: 299 3296 1477
Fragments arranged by size:
3296 1477 299
BsaAI yAC'GTr
Cuts at: 1347 2425 1347
Size: 1078 3994
BsaBI GATnn'nnATC
Cuts at: 6 2586 4623 6
Size: 2580 2037 455
Fragments arranged by size:
2580 2037 455
BsaHI Gr'CG_yC
Cuts at: 2503 2751 3453 4949 2503
Size: 248 702 1496 2626
Fragments arranged by size:
2626 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1870 2261 2782 3460 3734 3881 4292 1870
Size: 391 521 678 274 147 411 2650
Fragments arranged by size:
2650 678 521 411 391 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 232 262 1129 1159 1848 1878 232
Size: 30 867 30 689 30 3426
Fragments arranged by size:
3426 867 689 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 784 784
Size: 5072
BseYI C'CCAG_C
Cuts at: 1833 2497 3255 3832 4328 1833
Size: 664 758 577 496 2577
Fragments arranged by size:
2577 758 664 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 282 1972 224
Size: 58 1690 3324
Fragments arranged by size:
3324 1690 58
BsiEI CG_ry'CG
Cuts at: 104 2614 2660 3868 104
Size: 2510 46 1208 1308
Fragments arranged by size:
2510 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 137 292 563 650 835 1159 1283 1435 1589
Size: 155 271 87 185 324 124 152 154
Cuts at: 1589 2086 2864 3054 3846 137
Size: 497 778 190 792 1363
Fragments arranged by size:
1363 792 778 497 324 271 190 185
155 154 152 124 87
BsmI GAATG_Cn'
Cuts at: 156 891 156
Size: 735 4337
Bsp1286I G_dGCh'C
Cuts at: 137 236 292 563 620 650 835 1159 1283
Size: 99 56 271 57 30 185 324 124
Cuts at: 1283 1435 1589 1610 1956 2086 2697 2790 2864
Size: 152 154 21 346 130 611 93 74
Cuts at: 2864 3054 3116 3846 137
Size: 190 62 730 1363
Fragments arranged by size:
1363 730 611 346 324 271 190 185
154 152 130 124 99 93 74 62
57 56 30 21
BspEI T'CCGG_A
Cuts at: 4292 4292
Size: 5072
BspHI T'CATG_A
Cuts at: 854 1673 1911 4248 854
Size: 819 238 2337 1678
Fragments arranged by size:
2337 1678 819 238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 114 1087 1605 2122 2336 2638 3019 114
Size: 973 518 517 214 302 381 2167
Fragments arranged by size:
2167 973 518 517 381 302 214
BsrBI CCG'CTC
Cuts at: 50 882 3364 3418 4902 5067 50
Size: 832 2482 54 1484 165 55
Fragments arranged by size:
2482 1484 832 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2984 2984
Size: 5072
BsrFI r'CCGG_y
Cuts at: 1560 3070 3251 3460 1560
Size: 1510 181 209 3172
Fragments arranged by size:
3172 1510 209 181
BsrGI T'GTAC_A
Cuts at: 1370 1370
Size: 5072
BssSI C'ACGA_G
Cuts at: 258 1126 3343 3701 258
Size: 868 2217 358 1629
Fragments arranged by size:
2217 1629 868 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2449 2449
Size: 5072
BstBI TT'CG_AA
Cuts at: 3433 3433
Size: 5072
BstEII G'GTnAC_C
Cuts at: 1383 1552 1383
Size: 169 4903
BstXI CCAn_nnnn'nTGG
Cuts at: 1617 1617
Size: 5072
BstYI r'GATC_y
Cuts at: 1 1307 1496 2097 2564 2587 2922 3168 4180
Size: 1306 189 601 467 23 335 246 1012
Cuts at: 4180 4266 4278 4624 1
Size: 86 12 346 449
Fragments arranged by size:
1306 1012 601 467 449 346 335 246
189 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1505 1505
Size: 5072
BtgI C'CryG_G
Cuts at: 822 1490 1580 822
Size: 668 90 4314
Fragments arranged by size:
4314 668 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 195 3022 3203 3366 195
Size: 2827 181 163 1901
Fragments arranged by size:
2827 1901 181 163
BtsI GCAGTG_nn'
Cuts at: 1718 2315 1718
Size: 597 4475
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 991 1026 991
Size: 35 5037
DraI TTT'AAA
Cuts at: 2071 2071
Size: 5072
DrdI GACnn_nn'nnGTC
Cuts at: 2778 3636 2778
Size: 858 4214
EaeI y'GGCC_r
Cuts at: 473 1086 2657 2831 3222 3249 4594 473
Size: 613 1571 174 391 27 1345 951
Fragments arranged by size:
1571 1345 951 613 391 174 27
EagI C'GGCC_G
Cuts at: 2657 2657
Size: 5072
EarI CTCTTCn'nnn_
Cuts at: 948 3095 3305 948
Size: 2147 210 2715
Fragments arranged by size:
2715 2147 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 382 3280 3590 3736 4882 382
Size: 2898 310 146 1146 572
Fragments arranged by size:
2898 1146 572 310 146
EcoICRI GAG'CTC
Cuts at: 561 648 833 2084 561
Size: 87 185 1251 3549
Fragments arranged by size:
3549 1251 185 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 511 679 787 796 2897 3329 4076 4344
Size: 81 168 108 9 2101 432 747 268
Cuts at: 4344 430
Size: 1158
Fragments arranged by size:
2101 1158 747 432 268 168 108 81
9
EcoNI CCTnn'n_nnAGG
Cuts at: 307 1129 307
Size: 822 4250
EcoO109I rG'GnC_Cy
Cuts at: 233 300 312 414 458 530 1952 2211 233
Size: 67 12 102 44 72 1422 259 3094
Fragments arranged by size:
3094 1422 259 102 72 67 44 12
EcoRI G'AATT_C
Cuts at: 2076 2076
Size: 5072
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3091 3091
Size: 5072
FspI TGC'GCA
Cuts at: 2853 2853
Size: 5072
HaeII r_GCGC'y
Cuts at: 2506 2754 3776 4755 2506
Size: 248 1022 979 2823
Fragments arranged by size:
2823 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1129 1161 2914 2944 2946 2976 1129
Size: 32 1753 30 2 30 3225
Fragments arranged by size:
3225 1753 32 30 30 2
HincII GTy'rAC
Cuts at: 2111 2111
Size: 5072
KasI G'GCGC_C
Cuts at: 2502 2750 2502
Size: 248 4824
KpnI G_GTAC'C
Cuts at: 345 981 2092 345
Size: 636 1111 3325
Fragments arranged by size:
3325 1111 636
MluI A'CGCG_T
Cuts at: 4505 4505
Size: 5072
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1189 1210 1430 1478 3743 3927 1189
Size: 21 220 48 2265 184 2334
Fragments arranged by size:
2334 2265 220 184 48 21
MscI TGG'CCA
Cuts at: 475 2833 475
Size: 2358 2714
MslI CAynn'nnrTG
Cuts at: 370 1108 1366 1378 3061 3188 370
Size: 738 258 12 1683 127 2254
Fragments arranged by size:
2254 1683 738 258 127 12
MspA1I CmG'CkG
Cuts at: 972 1091 1776 2183 2497 2857 3870 4115 972
Size: 119 685 407 314 360 1013 245 1929
Fragments arranged by size:
1929 1013 685 407 360 314 245 119
NaeI GCC'GGC
Cuts at: 1562 3253 1562
Size: 1691 3381
NarI GG'CG_CC
Cuts at: 2503 2751 2503
Size: 248 4824
NcoI C'CATG_G
Cuts at: 822 822
Size: 5072
NgoMIV G'CCGG_C
Cuts at: 1560 3251 1560
Size: 1691 3381
NheI G'CTAG_C
Cuts at: 1038 1722 1038
Size: 684 4388
NsiI A_TGCA'T
Cuts at: 1381 1381
Size: 5072
NspI r_CATG'y
Cuts at: 723 741 1113 1379 2125 2625 3532 723
Size: 18 372 266 746 500 907 2263
Fragments arranged by size:
2263 907 746 500 372 266 18
PciI A'CATG_T
Cuts at: 3528 3528
Size: 5072
PflMI CCAn_nnn'nTGG
Cuts at: 408 1948 408
Size: 1540 3532
PmeI GTTT'AAAC
Cuts at: 2071 2071
Size: 5072
PmlI CAC'GTG
Cuts at: 1347 1347
Size: 5072
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 232 264 676 708 4211 4243 232
Size: 32 412 32 3503 32 1061
Fragments arranged by size:
3503 1061 412 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 312 414 312
Size: 102 4970
PspOMI G'GGCC_C
Cuts at: 232 1952 232
Size: 1720 3352
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 325 357 325
Size: 32 5040
PstI C_TGCA'G
Cuts at: 422 2119 2804 422
Size: 1697 685 2690
Fragments arranged by size:
2690 1697 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5072
PvuII CAG'CTG
Cuts at: 2497 2857 2497
Size: 360 4712
RsrII CG'GwC_CG
Cuts at: 3267 3267
Size: 5072
SacI G_AGCT'C
Cuts at: 563 650 835 2086 563
Size: 87 185 1251 3549
Fragments arranged by size:
3549 1251 185 87
SalI G'TCGA_C
Cuts at: 2109 2109
Size: 5072
SapI GCTCTTCn'nnn_
Cuts at: 3095 3305 3095
Size: 210 4862
SbfI CC_TGCA'GG
Cuts at: 2119 2119
Size: 5072
ScaI AGT'ACT
Cuts at: 4589 4589
Size: 5072
SexAI A'CCwGG_T
Cuts at: 980 980
Size: 5072
SfcI C'TryA_G
Cuts at: 33 418 1458 2115 2800 3793 3984 33
Size: 385 1040 657 685 993 191 1121
Fragments arranged by size:
1121 1040 993 685 657 385 191
SfoI GGC'GCC
Cuts at: 2504 2752 2504
Size: 248 4824
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5072
SmaI CCC'GGG
Cuts at: 2094 2094
Size: 5072
SmlI C'TyrA_G
Cuts at: 649 700 838 1184 1222 2227 3142 3634 3896
Size: 51 138 346 38 1005 915 492 262
Cuts at: 3896 649
Size: 1825
Fragments arranged by size:
1825 1005 915 492 346 262 138 51
38
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5072
SphI G_CATG'C
Cuts at: 2125 2625 2125
Size: 500 4572
StuI AGG'CCT
Cuts at: 810 1144 810
Size: 334 4738
StyI C'CwwG_G
Cuts at: 822 1009 1303 2039 2206 2560 822
Size: 187 294 736 167 354 3334
Fragments arranged by size:
3334 736 354 294 187 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1316 2655 2788 3247 3456 1316
Size: 1339 133 459 209 2932
Fragments arranged by size:
2932 1339 459 209 133
TatI w'GTAC_w
Cuts at: 1370 1523 1780 4587 1370
Size: 153 257 2807 1855
Fragments arranged by size:
2807 1855 257 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 186 218 4589 4621 186
Size: 32 4371 32 637
Fragments arranged by size:
4371 637 32 32
Tth111I GACn'n_nGTC
Cuts at: 2869 2869
Size: 5072
XbaI T'CTAG_A
Cuts at: 2103 2103
Size: 5072
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1479 2567 1479
Size: 1088 3984
XhoI C'TCGA_G
Cuts at: 1184 1184
Size: 5072
XmaI C'CCGG_G
Cuts at: 2092 2092
Size: 5072
XmnI GAAnn'nnTTC
Cuts at: 793 793
Size: 5072
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI
NspI PciI PflMI PmeI PmlI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AscI AvrII BaeI BcgI
BglI BsiWI BsmBI BssHII BstZ17I ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NotI NruI
PacI PasI PfoI PshAI PsiI PspXI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI ZraI