Restriction map for
pF1KB4959
(Circular) (Six-base) MAPSORT of: pF1KB4959.seq Check: 847 from: 1 to: 4817
pF1KB4959 4817 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AatII G_ACGT'C
Cuts at: 1626 1626
Size: 4817
AccI GT'mk_AC
Cuts at: 1481 1855 1481
Size: 374 4443
Acc65I G'GTAC_C
Cuts at: 1833 1833
Size: 4817
AclI AA'CG_TT
Cuts at: 4657 4657
Size: 4817
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 916 1117 2642 3074 3821 4089 430
Size: 486 201 1525 432 747 268 1158
Fragments arranged by size:
1525 1158 747 486 432 268 201
AfeI AGC'GCT
Cuts at: 814 814
Size: 4817
AflIII A'CryG_T
Cuts at: 2169 3273 4250 2169
Size: 1104 977 2736
Fragments arranged by size:
2736 1104 977
AgeI A'CCGG_T
Cuts at: 3205 3205
Size: 4817
AlwNI CAG_nnn'CTG
Cuts at: 644 800 1281 1332 2122 3689 644
Size: 156 481 51 790 1567 1772
Fragments arranged by size:
1772 1567 790 481 156 51
ApaLI G'TGCA_C
Cuts at: 764 1288 3587 764
Size: 524 2299 1994
Fragments arranged by size:
2299 1994 524
ApoI r'AATT_y
Cuts at: 434 1821 4170 4426 434
Size: 1387 2349 256 825
Fragments arranged by size:
2349 1387 825 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4817
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4817
AvaI C'yCGr_G
Cuts at: 1837 1837
Size: 4817
BamHI G'GATC_C
Cuts at: 1842 1842
Size: 4817
BanI G'GyrC_C
Cuts at: 314 1833 2247 2495 2530 314
Size: 1519 414 248 35 2601
Fragments arranged by size:
2601 1519 414 248 35
BanII G_rGCy'C
Cuts at: 355 1831 2861 355
Size: 1476 1030 2311
Fragments arranged by size:
2311 1476 1030
BbeI G_GCGC'C
Cuts at: 2251 2499 2251
Size: 248 4569
BbsI GAAGACnn'nnnn_
Cuts at: 247 692 1420 247
Size: 445 728 3644
Fragments arranged by size:
3644 728 445
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 745 779 897 931 1213 1247 745
Size: 34 118 34 282 34 4315
Fragments arranged by size:
4315 282 118 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1782 2715 3476 4817 1782
Size: 933 761 1341 1782
Fragments arranged by size:
1782 1341 933 761
BclI T'GATC_A
Cuts at: 2337 2337
Size: 4817
BfrBI ATG'CAT
Cuts at: 1639 1639
Size: 4817
BglI GCCn_nnn'nGGC
Cuts at: 862 1128 862
Size: 266 4551
BglII A'GATC_T
Cuts at: 449 2332 4369 449
Size: 1883 2037 897
Fragments arranged by size:
2037 1883 897
BlpI GC'TnA_GC
Cuts at: 349 1261 1929 349
Size: 912 668 3237
Fragments arranged by size:
3237 912 668
Bme1580I G_kGCm'C
Cuts at: 317 768 1292 2442 2535 3591 317
Size: 451 524 1150 93 1056 1543
Fragments arranged by size:
1543 1150 1056 524 451 93
BmgBI CAC'GTC
Cuts at: 885 885
Size: 4817
BmrI ACTGGGnnnn_n'
Cuts at: 1146 1705 2029 2110 2445 4589 1146
Size: 559 324 81 335 2144 1374
Fragments arranged by size:
2144 1374 559 335 324 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 885 3184 885
Size: 2299 2518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1162 1219 1270 1354 1703 1162
Size: 57 51 84 349 4276
Fragments arranged by size:
4276 349 84 57 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 600 1993 2872 3364 3662 600
Size: 1393 879 492 298 1755
Fragments arranged by size:
1755 1393 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 590 1330 4521 590
Size: 740 3191 886
Fragments arranged by size:
3191 886 740
BsaAI yAC'GTr
Cuts at: 603 2170 603
Size: 1567 3250
BsaBI GATnn'nnATC
Cuts at: 6 1693 2331 4368 6
Size: 1687 638 2037 455
Fragments arranged by size:
2037 1687 638 455
BsaHI Gr'CG_yC
Cuts at: 193 1623 2248 2496 3198 4694 193
Size: 1430 625 248 702 1496 316
Fragments arranged by size:
1496 1430 702 625 316 248
BsaWI w'CCGG_w
Cuts at: 2006 2527 3205 3479 3626 4037 2006
Size: 521 678 274 147 411 2786
Fragments arranged by size:
2786 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 241 271 598 628 241
Size: 30 327 30 4430
Fragments arranged by size:
4430 327 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 765 765
Size: 4817
BseYI C'CCAG_C
Cuts at: 187 495 793 1256 2242 3000 3577 4073 187
Size: 308 298 463 986 758 577 496 931
Fragments arranged by size:
986 931 758 577 496 463 308 298
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 309 500 750 898 154
Size: 155 191 250 148 4073
Fragments arranged by size:
4073 250 191 155 148
BsiEI CG_ry'CG
Cuts at: 104 2359 2405 3613 104
Size: 2255 46 1208 1308
Fragments arranged by size:
2255 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 355 768 1292 1361 1831 2609 2799 3591 355
Size: 413 524 69 470 778 190 792 1581
Fragments arranged by size:
1581 792 778 524 470 413 190 69
BsmI GAATG_Cn'
Cuts at: 1639 1639
Size: 4817
Bsp1286I G_dGCh'C
Cuts at: 317 355 768 1292 1361 1831 2442 2535 2609
Size: 38 413 524 69 470 611 93 74
Cuts at: 2609 2799 2861 3591 317
Size: 190 62 730 1543
Fragments arranged by size:
1543 730 611 524 470 413 190 93
74 69 62 38
BspEI T'CCGG_A
Cuts at: 4037 4037
Size: 4817
BspHI T'CATG_A
Cuts at: 3993 3993
Size: 4817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 599 899 1325 1867 2081 2383 2764 599
Size: 300 426 542 214 302 381 2652
Fragments arranged by size:
2652 542 426 381 302 300 214
BsrBI CCG'CTC
Cuts at: 50 3109 3163 4647 4812 50
Size: 3059 54 1484 165 55
Fragments arranged by size:
3059 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2729 2729
Size: 4817
BsrFI r'CCGG_y
Cuts at: 1265 2815 2996 3205 1265
Size: 1550 181 209 2877
Fragments arranged by size:
2877 1550 209 181
BsrGI T'GTAC_A
Cuts at: 370 370
Size: 4817
BssHII G'CGCG_C
Cuts at: 140 140
Size: 4817
BssSI C'ACGA_G
Cuts at: 859 3088 3446 859
Size: 2229 358 2230
Fragments arranged by size:
2230 2229 358
BstAPI GCAn_nnn'nTGC
Cuts at: 492 644 2194 492
Size: 152 1550 3115
Fragments arranged by size:
3115 1550 152
BstBI TT'CG_AA
Cuts at: 1035 3178 1035
Size: 2143 2674
BstYI r'GATC_y
Cuts at: 1 449 1115 1797 1842 2309 2332 2667 2913
Size: 448 666 682 45 467 23 335 246
Cuts at: 2913 3925 4011 4023 4369 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 682 666 467 449 448 346 335
246 86 45 23 12
Bsu36I CC'TnA_GG
Cuts at: 748 748
Size: 4817
BtgI C'CryG_G
Cuts at: 107 245 107
Size: 138 4679
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2767 2948 3111 2767
Size: 181 163 4473
Fragments arranged by size:
4473 181 163
BtsI GCAGTG_nn'
Cuts at: 189 642 2060 189
Size: 453 1418 2946
Fragments arranged by size:
2946 1418 453
DraI TTT'AAA
Cuts at: 518 1816 518
Size: 1298 3519
DraIII CAC_nnn'GTG
Cuts at: 282 282
Size: 4817
DrdI GACnn_nn'nnGTC
Cuts at: 1518 2523 3381 1518
Size: 1005 858 2954
Fragments arranged by size:
2954 1005 858
EaeI y'GGCC_r
Cuts at: 1570 2402 2576 2967 2994 4339 1570
Size: 832 174 391 27 1345 2048
Fragments arranged by size:
2048 1345 832 391 174 27
EagI C'GGCC_G
Cuts at: 2402 2402
Size: 4817
EarI CTCTTCn'nnn_
Cuts at: 360 449 1153 2840 3050 360
Size: 89 704 1687 210 2127
Fragments arranged by size:
2127 1687 704 210 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 861 1288 1404 3025 3335 3481 4627 861
Size: 427 116 1621 310 146 1146 1051
Fragments arranged by size:
1621 1146 1051 427 310 146 116
EcoICRI GAG'CTC
Cuts at: 353 1829 353
Size: 1476 3341
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 916 1117 1162 1219 1270 1354 1703 2642
Size: 486 201 45 57 51 84 349 939
Cuts at: 2642 3074 3821 4089 430
Size: 432 747 268 1158
Fragments arranged by size:
1158 939 747 486 432 349 268 201
84 57 51 45
EcoNI CCTnn'n_nnAGG
Cuts at: 212 895 212
Size: 683 4134
EcoO109I rG'GnC_Cy
Cuts at: 1329 1613 1956 1329
Size: 284 343 4190
Fragments arranged by size:
4190 343 284
EcoRI G'AATT_C
Cuts at: 1821 1821
Size: 4817
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 948 2836 948
Size: 1888 2929
FspI TGC'GCA
Cuts at: 2598 2598
Size: 4817
HaeII r_GCGC'y
Cuts at: 816 2251 2499 3521 4500 816
Size: 1435 248 1022 979 1133
Fragments arranged by size:
1435 1133 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 117 149 441 473 1346 1378 1597 1629 2659
Size: 32 292 32 873 32 219 32 1030
Cuts at: 2659 2689 2691 2721 117
Size: 30 2 30 2213
Fragments arranged by size:
2213 1030 873 292 219 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 888 1856 888
Size: 968 3849
KasI G'GCGC_C
Cuts at: 2247 2495 2247
Size: 248 4569
KpnI G_GTAC'C
Cuts at: 1837 1837
Size: 4817
MluI A'CGCG_T
Cuts at: 4250 4250
Size: 4817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 439 3488 3672 439
Size: 3049 184 1584
Fragments arranged by size:
3049 1584 184
MscI TGG'CCA
Cuts at: 2578 2578
Size: 4817
MslI CAynn'nnrTG
Cuts at: 1600 2806 2933 1600
Size: 1206 127 3484
Fragments arranged by size:
3484 1206 127
MspA1I CmG'CkG
Cuts at: 1260 1928 2242 2602 3615 3860 1260
Size: 668 314 360 1013 245 2217
Fragments arranged by size:
2217 1013 668 360 314 245
NaeI GCC'GGC
Cuts at: 1267 2998 1267
Size: 1731 3086
NarI GG'CG_CC
Cuts at: 2248 2496 2248
Size: 248 4569
NcoI C'CATG_G
Cuts at: 107 107
Size: 4817
NgoMIV G'CCGG_C
Cuts at: 1265 2996 1265
Size: 1731 3086
NruI TCG'CGA
Cuts at: 1376 1376
Size: 4817
NsiI A_TGCA'T
Cuts at: 1641 1641
Size: 4817
NspI r_CATG'y
Cuts at: 638 1870 2370 3277 638
Size: 1232 500 907 2178
Fragments arranged by size:
2178 1232 907 500
PciI A'CATG_T
Cuts at: 3273 3273
Size: 4817
PflMI CCAn_nnn'nTGG
Cuts at: 1332 1332
Size: 4817
PfoI T'CCnGG_A
Cuts at: 1331 1331
Size: 4817
PmeI GTTT'AAAC
Cuts at: 1816 1816
Size: 4817
PmlI CAC'GTG
Cuts at: 603 603
Size: 4817
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3988 3956
Size: 32 4785
PpuMI rG'GwC_Cy
Cuts at: 1329 1613 1329
Size: 284 4533
PstI C_TGCA'G
Cuts at: 149 677 1864 2549 149
Size: 528 1187 685 2417
Fragments arranged by size:
2417 1187 685 528
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4817
PvuII CAG'CTG
Cuts at: 1260 2242 2602 1260
Size: 982 360 3475
Fragments arranged by size:
3475 982 360
RsrII CG'GwC_CG
Cuts at: 3012 3012
Size: 4817
SacI G_AGCT'C
Cuts at: 355 1831 355
Size: 1476 3341
SalI G'TCGA_C
Cuts at: 1854 1854
Size: 4817
SapI GCTCTTCn'nnn_
Cuts at: 360 449 1153 2840 3050 360
Size: 89 704 1687 210 2127
Fragments arranged by size:
2127 1687 704 210 89
SbfI CC_TGCA'GG
Cuts at: 1864 1864
Size: 4817
ScaI AGT'ACT
Cuts at: 4334 4334
Size: 4817
SexAI A'CCwGG_T
Cuts at: 278 278
Size: 4817
SfcI C'TryA_G
Cuts at: 33 145 673 801 1860 2545 3538 3729 33
Size: 112 528 128 1059 685 993 191 1121
Fragments arranged by size:
1121 1059 993 685 528 191 128 112
SfiI GGCCn_nnn'nGGCC
Cuts at: 862 862
Size: 4817
SfoI GGC'GCC
Cuts at: 2249 2497 2249
Size: 248 4569
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4817
SmaI CCC'GGG
Cuts at: 1839 1839
Size: 4817
SmlI C'TyrA_G
Cuts at: 579 1972 2887 3379 3641 579
Size: 1393 915 492 262 1755
Fragments arranged by size:
1755 1393 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4817
SphI G_CATG'C
Cuts at: 1870 2370 1870
Size: 500 4317
StuI AGG'CCT
Cuts at: 1244 1244
Size: 4817
StyI C'CwwG_G
Cuts at: 107 1567 1951 2305 107
Size: 1460 384 354 2619
Fragments arranged by size:
2619 1460 384 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 319 1207 2400 2533 2992 3201 319
Size: 888 1193 133 459 209 1935
Fragments arranged by size:
1935 1193 888 459 209 133
TatI w'GTAC_w
Cuts at: 257 370 4332 257
Size: 113 3962 742
Fragments arranged by size:
3962 742 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 598 630 681 713 4334 4366 598
Size: 32 51 32 3621 32 1049
Fragments arranged by size:
3621 1049 51 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1419 2614 1419
Size: 1195 3622
XbaI T'CTAG_A
Cuts at: 1734 1848 1734
Size: 114 4703
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2312 2312
Size: 4817
XmaI C'CCGG_G
Cuts at: 1837 1837
Size: 4817
XmnI GAAnn'nnTTC
Cuts at: 542 1031 542
Size: 489 4328
ZraI GAC'GTC
Cuts at: 1624 1624
Size: 4817
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NsiI NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BmtI Bpu10I BsiWI BsmBI BstEII
BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI PacI PasI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SgrAI SnaBI
SrfI SspI SwaI XhoI