Restriction map for
pF1KB4945
(Circular) (Six-base) MAPSORT of: pF1KB4945.seq Check: 979 from: 1 to: 4049
pF1KB4945 4049 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 512 889 1087 512
Size: 377 198 3474
Fragments arranged by size:
3474 377 198
Acc65I G'GTAC_C
Cuts at: 1065 1065
Size: 4049
AclI AA'CG_TT
Cuts at: 3889 3889
Size: 4049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 1874 2306 3053 3321 430
Size: 1444 432 747 268 1158
Fragments arranged by size:
1444 1158 747 432 268
AflIII A'CryG_T
Cuts at: 437 1401 2505 3482 437
Size: 964 1104 977 1004
Fragments arranged by size:
1104 1004 977 964
AgeI A'CCGG_T
Cuts at: 2437 2437
Size: 4049
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 358 390 358
Size: 32 4017
AlwNI CAG_nnn'CTG
Cuts at: 408 1354 2921 408
Size: 946 1567 1536
Fragments arranged by size:
1567 1536 946
ApaI G_GGCC'C
Cuts at: 646 646
Size: 4049
ApaLI G'TGCA_C
Cuts at: 2819 2819
Size: 4049
ApoI r'AATT_y
Cuts at: 279 1053 3402 3658 279
Size: 774 2349 256 670
Fragments arranged by size:
2349 774 670 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
AvaI C'yCGr_G
Cuts at: 1069 1069
Size: 4049
BamHI G'GATC_C
Cuts at: 1074 1074
Size: 4049
BanI G'GyrC_C
Cuts at: 528 1065 1479 1727 1762 528
Size: 537 414 248 35 2815
Fragments arranged by size:
2815 537 414 248 35
BanII G_rGCy'C
Cuts at: 293 646 1063 2093 293
Size: 353 417 1030 2249
Fragments arranged by size:
2249 1030 417 353
BbeI G_GCGC'C
Cuts at: 1483 1731 1483
Size: 248 3801
BbsI GAAGACnn'nnnn_
Cuts at: 886 886
Size: 4049
BciVI GTATCCnnnnn_n'
Cuts at: 1947 2708 4049 1947
Size: 761 1341 1947
Fragments arranged by size:
1947 1341 761
BclI T'GATC_A
Cuts at: 1569 1569
Size: 4049
BglII A'GATC_T
Cuts at: 1564 3601 1564
Size: 2037 2012
BlpI GC'TnA_GC
Cuts at: 1161 1161
Size: 4049
Bme1580I G_kGCm'C
Cuts at: 646 805 1674 1767 2823 646
Size: 159 869 93 1056 1872
Fragments arranged by size:
1872 1056 869 159 93
BmgBI CAC'GTC
Cuts at: 147 147
Size: 4049
BmrI ACTGGGnnnn_n'
Cuts at: 815 1261 1342 1677 3821 815
Size: 446 81 335 2144 1043
Fragments arranged by size:
2144 1043 446 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 994 277
Size: 717 3332
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1225 2104 2596 2894 847
Size: 378 879 492 298 2002
Fragments arranged by size:
2002 879 492 378 298
BsaI GGTCTCn'nnnn_
Cuts at: 3753 3753
Size: 4049
BsaAI yAC'GTr
Cuts at: 162 345 438 1402 162
Size: 183 93 964 2809
Fragments arranged by size:
2809 964 183 93
BsaBI GATnn'nnATC
Cuts at: 6 1563 3600 6
Size: 1557 2037 455
Fragments arranged by size:
2037 1557 455
BsaHI Gr'CG_yC
Cuts at: 788 1480 1728 2430 3926 788
Size: 692 248 702 1496 911
Fragments arranged by size:
1496 911 702 692 248
BsaWI w'CCGG_w
Cuts at: 764 1238 1759 2437 2711 2858 3269 764
Size: 474 521 678 274 147 411 1544
Fragments arranged by size:
1544 678 521 474 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 664 694 103
Size: 30 531 30 3458
Fragments arranged by size:
3458 531 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 130 738 741 127
Size: 3 608 3 3435
Fragments arranged by size:
3435 608 3 3
BseYI C'CCAG_C
Cuts at: 711 958 1474 2232 2809 3305 711
Size: 247 516 758 577 496 1455
Fragments arranged by size:
1455 758 577 516 496 247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 616 1009 421
Size: 195 393 3461
Fragments arranged by size:
3461 393 195
BsiEI CG_ry'CG
Cuts at: 104 1591 1637 2845 104
Size: 1487 46 1208 1308
Fragments arranged by size:
1487 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 131 293 350 1063 1841 2031 2823 131
Size: 162 57 713 778 190 792 1357
Fragments arranged by size:
1357 792 778 713 190 162 57
Bsp1286I G_dGCh'C
Cuts at: 131 293 350 646 805 1063 1674 1767 1841
Size: 162 57 296 159 258 611 93 74
Cuts at: 1841 2031 2093 2823 131
Size: 190 62 730 1357
Fragments arranged by size:
1357 730 611 296 258 190 162 159
93 74 62 57
BspEI T'CCGG_A
Cuts at: 764 3269 764
Size: 2505 1544
BspHI T'CATG_A
Cuts at: 3225 3225
Size: 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 183 683 1099 1313 1615 1996 183
Size: 500 416 214 302 381 2236
Fragments arranged by size:
2236 500 416 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2341 2395 3879 4044 50
Size: 2291 54 1484 165 55
Fragments arranged by size:
2291 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1961 1961
Size: 4049
BsrFI r'CCGG_y
Cuts at: 2047 2228 2437 2047
Size: 181 209 3659
Fragments arranged by size:
3659 209 181
BssSI C'ACGA_G
Cuts at: 2320 2678 2320
Size: 358 3691
BstAPI GCAn_nnn'nTGC
Cuts at: 1426 1426
Size: 4049
BstBI TT'CG_AA
Cuts at: 2410 2410
Size: 4049
BstXI CCAn_nnnn'nTGG
Cuts at: 390 831 972 1041 390
Size: 441 141 69 3398
Fragments arranged by size:
3398 441 141 69
BstYI r'GATC_y
Cuts at: 1 590 1074 1541 1564 1899 2145 3157 3243
Size: 589 484 467 23 335 246 1012 86
Cuts at: 3243 3255 3601 1
Size: 12 346 449
Fragments arranged by size:
1012 589 484 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 313 313
Size: 4049
BtgI C'CryG_G
Cuts at: 107 153 383 653 746 1034 107
Size: 46 230 270 93 288 3122
Fragments arranged by size:
3122 288 270 230 93 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1999 2180 2343 1999
Size: 181 163 3705
Fragments arranged by size:
3705 181 163
BtsI GCAGTG_nn'
Cuts at: 1292 1292
Size: 4049
DraI TTT'AAA
Cuts at: 1048 1048
Size: 4049
DrdI GACnn_nn'nnGTC
Cuts at: 655 1755 2613 655
Size: 1100 858 2091
Fragments arranged by size:
2091 1100 858
EaeI y'GGCC_r
Cuts at: 176 380 582 1634 1808 2199 2226 3571 176
Size: 204 202 1052 174 391 27 1345 654
Fragments arranged by size:
1345 1052 654 391 204 202 174 27
EagI C'GGCC_G
Cuts at: 1634 1634
Size: 4049
EarI CTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2257 2567 2713 3859 2257
Size: 310 146 1146 2447
Fragments arranged by size:
2447 1146 310 146
EcoICRI GAG'CTC
Cuts at: 291 1061 291
Size: 770 3279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 430 994 1874 2306 3053 3321 277
Size: 153 564 880 432 747 268 1005
Fragments arranged by size:
1005 880 747 564 432 268 153
EcoO109I rG'GnC_Cy
Cuts at: 498 643 810 1011 1188 498
Size: 145 167 201 177 3359
Fragments arranged by size:
3359 201 177 167 145
EcoRI G'AATT_C
Cuts at: 279 1053 279
Size: 774 3275
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 4049
FspI TGC'GCA
Cuts at: 1830 1830
Size: 4049
HaeII r_GCGC'y
Cuts at: 1483 1731 2753 3732 1483
Size: 248 1022 979 1800
Fragments arranged by size:
1800 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 664 696 717 749 1891 1921 1923 1953 664
Size: 32 21 32 1142 30 2 30 2760
Fragments arranged by size:
2760 1142 32 32 30 30 21 2
HincII GTy'rAC
Cuts at: 1088 1088
Size: 4049
KasI G'GCGC_C
Cuts at: 1479 1727 1479
Size: 248 3801
KpnI G_GTAC'C
Cuts at: 1069 1069
Size: 4049
MluI A'CGCG_T
Cuts at: 3482 3482
Size: 4049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 695 2720 2904 695
Size: 2025 184 1840
Fragments arranged by size:
2025 1840 184
MscI TGG'CCA
Cuts at: 382 1810 382
Size: 1428 2621
MslI CAynn'nnrTG
Cuts at: 388 766 2038 2165 388
Size: 378 1272 127 2272
Fragments arranged by size:
2272 1272 378 127
MspA1I CmG'CkG
Cuts at: 155 189 739 972 1160 1474 1834 2847 3092
Size: 34 550 233 188 314 360 1013 245
Cuts at: 3092 155
Size: 1112
Fragments arranged by size:
1112 1013 550 360 314 245 233 188
34
NaeI GCC'GGC
Cuts at: 2230 2230
Size: 4049
NarI GG'CG_CC
Cuts at: 1480 1728 1480
Size: 248 3801
NcoI C'CATG_G
Cuts at: 107 383 107
Size: 276 3773
NgoMIV G'CCGG_C
Cuts at: 2228 2228
Size: 4049
NspI r_CATG'y
Cuts at: 630 1102 1602 2509 630
Size: 472 500 907 2170
Fragments arranged by size:
2170 907 500 472
PciI A'CATG_T
Cuts at: 2505 2505
Size: 4049
PfoI T'CCnGG_A
Cuts at: 293 491 293
Size: 198 3851
PmeI GTTT'AAAC
Cuts at: 1048 1048
Size: 4049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4017
PpuMI rG'GwC_Cy
Cuts at: 498 810 1011 498
Size: 312 201 3536
Fragments arranged by size:
3536 312 201
PspOMI G'GGCC_C
Cuts at: 642 642
Size: 4049
PstI C_TGCA'G
Cuts at: 194 911 1096 1781 194
Size: 717 185 685 2462
Fragments arranged by size:
2462 717 685 185
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4049
PvuII CAG'CTG
Cuts at: 972 1474 1834 972
Size: 502 360 3187
Fragments arranged by size:
3187 502 360
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 4049
SacI G_AGCT'C
Cuts at: 293 1063 293
Size: 770 3279
SacII CC_GC'GG
Cuts at: 156 156
Size: 4049
SalI G'TCGA_C
Cuts at: 1086 1086
Size: 4049
SapI GCTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
SbfI CC_TGCA'GG
Cuts at: 1096 1096
Size: 4049
ScaI AGT'ACT
Cuts at: 3566 3566
Size: 4049
SfcI C'TryA_G
Cuts at: 33 190 907 1092 1777 2770 2961 33
Size: 157 717 185 685 993 191 1121
Fragments arranged by size:
1121 993 717 685 191 185 157
SfoI GGC'GCC
Cuts at: 1481 1729 1481
Size: 248 3801
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
SmaI CCC'GGG
Cuts at: 1071 1071
Size: 4049
SmlI C'TyrA_G
Cuts at: 862 1204 2119 2611 2873 862
Size: 342 915 492 262 2038
Fragments arranged by size:
2038 915 492 342 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4049
SphI G_CATG'C
Cuts at: 1102 1602 1102
Size: 500 3549
StuI AGG'CCT
Cuts at: 311 311
Size: 4049
StyI C'CwwG_G
Cuts at: 107 383 1183 1537 107
Size: 276 800 354 2619
Fragments arranged by size:
2619 800 354 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 445 1632 1765 2224 2433 445
Size: 1187 133 459 209 2061
Fragments arranged by size:
2061 1187 459 209 133
TatI w'GTAC_w
Cuts at: 3564 3564
Size: 4049
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 3566 3598 103
Size: 32 3431 32 554
Fragments arranged by size:
3431 554 32 32
Tth111I GACn'n_nGTC
Cuts at: 790 885 1846 790
Size: 95 961 2993
Fragments arranged by size:
2993 961 95
XbaI T'CTAG_A
Cuts at: 1080 1080
Size: 4049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1544 1544
Size: 4049
XmaI C'CCGG_G
Cuts at: 1069 1069
Size: 4049
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI