Restriction map for
pF1KB4900
(Circular) (Six-base) MAPSORT of: pF1KB4900.seq Check: 7364 from: 1 to: 4739
pF1KB4900 4739 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 1777 1777
Size: 4739
Acc65I G'GTAC_C
Cuts at: 1755 1755
Size: 4739
AclI AA'CG_TT
Cuts at: 4579 4579
Size: 4739
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1633 2564 2996 3743 4011 1633
Size: 931 432 747 268 2361
Fragments arranged by size:
2361 931 747 432 268
AflIII A'CryG_T
Cuts at: 635 2091 3195 4172 635
Size: 1456 1104 977 1202
Fragments arranged by size:
1456 1202 1104 977
AgeI A'CCGG_T
Cuts at: 3127 3127
Size: 4739
AlwNI CAG_nnn'CTG
Cuts at: 390 817 906 2044 3611 390
Size: 427 89 1138 1567 1518
Fragments arranged by size:
1567 1518 1138 427 89
ApaLI G'TGCA_C
Cuts at: 3509 3509
Size: 4739
ApoI r'AATT_y
Cuts at: 1433 1743 4092 4348 1433
Size: 310 2349 256 1824
Fragments arranged by size:
2349 1824 310 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4739
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4739
AvaI C'yCGr_G
Cuts at: 1759 1759
Size: 4739
BamHI G'GATC_C
Cuts at: 1764 1764
Size: 4739
BanI G'GyrC_C
Cuts at: 1755 2169 2417 2452 1755
Size: 414 248 35 4042
Fragments arranged by size:
4042 414 248 35
BanII G_rGCy'C
Cuts at: 134 1753 2783 134
Size: 1619 1030 2090
Fragments arranged by size:
2090 1619 1030
BbeI G_GCGC'C
Cuts at: 2173 2421 2173
Size: 248 4491
BbsI GAAGACnn'nnnn_
Cuts at: 843 1028 843
Size: 185 4554
BciVI GTATCCnnnnn_n'
Cuts at: 2637 3398 4739 2637
Size: 761 1341 2637
Fragments arranged by size:
2637 1341 761
BclI T'GATC_A
Cuts at: 626 2259 626
Size: 1633 3106
BglII A'GATC_T
Cuts at: 2254 4291 2254
Size: 2037 2702
BlpI GC'TnA_GC
Cuts at: 1851 1851
Size: 4739
Bme1580I G_kGCm'C
Cuts at: 559 713 2364 2457 3513 559
Size: 154 1651 93 1056 1785
Fragments arranged by size:
1785 1651 1056 154 93
BmrI ACTGGGnnnn_n'
Cuts at: 799 1570 1951 2032 2367 4511 799
Size: 771 381 81 335 2144 1027
Fragments arranged by size:
2144 1027 771 381 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3106 3106
Size: 4739
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 396
Size: 4739
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1334 1915 2794 3286 3584 1334
Size: 581 879 492 298 2489
Fragments arranged by size:
2489 879 581 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4443 4443
Size: 4739
BsaAI yAC'GTr
Cuts at: 2092 2092
Size: 4739
BsaBI GATnn'nnATC
Cuts at: 6 2253 4290 6
Size: 2247 2037 455
Fragments arranged by size:
2247 2037 455
BsaHI Gr'CG_yC
Cuts at: 2170 2418 3120 4616 2170
Size: 248 702 1496 2293
Fragments arranged by size:
2293 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1541 1928 2449 3127 3401 3548 3959 1541
Size: 387 521 678 274 147 411 2321
Fragments arranged by size:
2321 678 521 411 387 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1582 1612 1582
Size: 30 4709
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 808 1102 808
Size: 294 4445
BseYI C'CCAG_C
Cuts at: 1000 1579 2164 2922 3499 3995 1000
Size: 579 585 758 577 496 1744
Fragments arranged by size:
1744 758 585 579 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 226
Size: 4739
BsiEI CG_ry'CG
Cuts at: 104 2281 2327 3535 104
Size: 2177 46 1208 1308
Fragments arranged by size:
2177 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1280 1595 1753 2531 2721 3513 1280
Size: 315 158 778 190 792 2506
Fragments arranged by size:
2506 792 778 315 190 158
BsmI GAATG_Cn'
Cuts at: 255 473 255
Size: 218 4521
Bsp1286I G_dGCh'C
Cuts at: 134 559 713 1280 1595 1753 2364 2457 2531
Size: 425 154 567 315 158 611 93 74
Cuts at: 2531 2721 2783 3513 134
Size: 190 62 730 1360
Fragments arranged by size:
1360 730 611 567 425 315 190 158
154 93 74 62
BspEI T'CCGG_A
Cuts at: 1541 3959 1541
Size: 2418 2321
BspHI T'CATG_A
Cuts at: 941 3915 941
Size: 2974 1765
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1789 2003 2305 2686 1789
Size: 214 302 381 3842
Fragments arranged by size:
3842 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1183 3031 3085 4569 4734 50
Size: 1133 1848 54 1484 165 55
Fragments arranged by size:
1848 1484 1133 165 55 54
BsrDI GCAATG_nn'
Cuts at: 126 503 1343 2651 126
Size: 377 840 1308 2214
Fragments arranged by size:
2214 1308 840 377
BsrFI r'CCGG_y
Cuts at: 2737 2918 3127 2737
Size: 181 209 4349
Fragments arranged by size:
4349 209 181
BsrGI T'GTAC_A
Cuts at: 1055 1055
Size: 4739
BssSI C'ACGA_G
Cuts at: 3010 3368 3010
Size: 358 4381
BstAPI GCAn_nnn'nTGC
Cuts at: 2116 2116
Size: 4739
BstBI TT'CG_AA
Cuts at: 3100 3100
Size: 4739
BstXI CCAn_nnnn'nTGG
Cuts at: 1302 1560 1302
Size: 258 4481
BstYI r'GATC_y
Cuts at: 1 814 1764 2231 2254 2589 2835 3847 3933
Size: 813 950 467 23 335 246 1012 86
Cuts at: 3933 3945 4291 1
Size: 12 346 449
Fragments arranged by size:
1012 950 813 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1439 1439
Size: 4739
BtgI C'CryG_G
Cuts at: 767 977 767
Size: 210 4529
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1497 2689 2870 3033 1497
Size: 1192 181 163 3203
Fragments arranged by size:
3203 1192 181 163
BtsI GCAGTG_nn'
Cuts at: 1982 1982
Size: 4739
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 486 521 486
Size: 35 4704
DraI TTT'AAA
Cuts at: 669 678 705 1738 669
Size: 9 27 1033 3670
Fragments arranged by size:
3670 1033 27 9
DrdI GACnn_nn'nnGTC
Cuts at: 1261 2445 3303 1261
Size: 1184 858 2697
Fragments arranged by size:
2697 1184 858
EaeI y'GGCC_r
Cuts at: 191 2324 2498 2889 2916 4261 191
Size: 2133 174 391 27 1345 669
Fragments arranged by size:
2133 1345 669 391 174 27
EagI C'GGCC_G
Cuts at: 2324 2324
Size: 4739
EarI CTCTTCn'nnn_
Cuts at: 122 688 994 1200 1232 1646 2762 2972 122
Size: 566 306 206 32 414 1116 210 1889
Fragments arranged by size:
1889 1116 566 414 306 210 206 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2947 3257 3403 4549 2947
Size: 310 146 1146 3137
Fragments arranged by size:
3137 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1751 1751
Size: 4739
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 1633 2564 2996 3743 4011 396
Size: 1237 931 432 747 268 1124
Fragments arranged by size:
1237 1124 931 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1321 1321
Size: 4739
EcoO109I rG'GnC_Cy
Cuts at: 903 1190 1878 903
Size: 287 688 3764
Fragments arranged by size:
3764 688 287
EcoRI G'AATT_C
Cuts at: 1433 1743 1433
Size: 310 4429
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 717 2758 717
Size: 2041 2698
FspI TGC'GCA
Cuts at: 2520 2520
Size: 4739
HaeII r_GCGC'y
Cuts at: 439 2173 2421 3443 4422 439
Size: 1734 248 1022 979 756
Fragments arranged by size:
1734 1022 979 756 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 293 325 464 496 1262 1294 1582 1614 2581
Size: 32 139 32 766 32 288 32 967
Cuts at: 2581 2611 2613 2643 293
Size: 30 2 30 2389
Fragments arranged by size:
2389 967 766 288 139 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1356 1778 1356
Size: 422 4317
HindIII A'AGCT_T
Cuts at: 699 699
Size: 4739
HpaI GTT'AAC
Cuts at: 1356 1356
Size: 4739
KasI G'GCGC_C
Cuts at: 2169 2417 2169
Size: 248 4491
KpnI G_GTAC'C
Cuts at: 1759 1759
Size: 4739
MluI A'CGCG_T
Cuts at: 4172 4172
Size: 4739
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 336 3410 3594 336
Size: 3074 184 1481
Fragments arranged by size:
3074 1481 184
MscI TGG'CCA
Cuts at: 193 2500 193
Size: 2307 2432
MslI CAynn'nnrTG
Cuts at: 427 508 1030 1300 1516 2728 2855 427
Size: 81 522 270 216 1212 127 2311
Fragments arranged by size:
2311 1212 522 270 216 127 81
MspA1I CmG'CkG
Cuts at: 277 604 1411 1429 1850 2164 2524 3537 3782
Size: 327 807 18 421 314 360 1013 245
Cuts at: 3782 277
Size: 1234
Fragments arranged by size:
1234 1013 807 421 360 327 314 245
18
NaeI GCC'GGC
Cuts at: 2920 2920
Size: 4739
NarI GG'CG_CC
Cuts at: 2170 2418 2170
Size: 248 4491
NcoI C'CATG_G
Cuts at: 767 767
Size: 4739
NgoMIV G'CCGG_C
Cuts at: 2918 2918
Size: 4739
NspI r_CATG'y
Cuts at: 573 639 1792 2292 3199 573
Size: 66 1153 500 907 2113
Fragments arranged by size:
2113 1153 907 500 66
PasI CC'CwG_GG
Cuts at: 837 1572 837
Size: 735 4004
PciI A'CATG_T
Cuts at: 635 3195 635
Size: 2560 2179
PfoI T'CCnGG_A
Cuts at: 617 810 1529 617
Size: 193 719 3827
Fragments arranged by size:
3827 719 193
PmeI GTTT'AAAC
Cuts at: 1738 1738
Size: 4739
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 3878
Size: 32 4707
PpuMI rG'GwC_Cy
Cuts at: 1190 1190
Size: 4739
PshAI GACnn'nnGTC
Cuts at: 1477 1477
Size: 4739
PstI C_TGCA'G
Cuts at: 609 1786 2471 609
Size: 1177 685 2877
Fragments arranged by size:
2877 1177 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4739
PvuII CAG'CTG
Cuts at: 604 1411 1429 2164 2524 604
Size: 807 18 735 360 2819
Fragments arranged by size:
2819 807 735 360 18
RsrII CG'GwC_CG
Cuts at: 2934 2934
Size: 4739
SacI G_AGCT'C
Cuts at: 1753 1753
Size: 4739
SalI G'TCGA_C
Cuts at: 1776 1776
Size: 4739
SapI GCTCTTCn'nnn_
Cuts at: 122 2762 2972 122
Size: 2640 210 1889
Fragments arranged by size:
2640 1889 210
SbfI CC_TGCA'GG
Cuts at: 1786 1786
Size: 4739
ScaI AGT'ACT
Cuts at: 346 4256 346
Size: 3910 829
SfcI C'TryA_G
Cuts at: 33 476 605 1782 2467 3460 3651 33
Size: 443 129 1177 685 993 191 1121
Fragments arranged by size:
1177 1121 993 685 443 191 129
SfoI GGC'GCC
Cuts at: 2171 2419 2171
Size: 248 4491
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4739
SmaI CCC'GGG
Cuts at: 1761 1761
Size: 4739
SmlI C'TyrA_G
Cuts at: 1349 1894 2809 3301 3563 1349
Size: 545 915 492 262 2525
Fragments arranged by size:
2525 915 545 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4739
SphI G_CATG'C
Cuts at: 1792 2292 1792
Size: 500 4239
StyI C'CwwG_G
Cuts at: 297 767 1141 1873 2227 297
Size: 470 374 732 354 2809
Fragments arranged by size:
2809 732 470 374 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 727 1018 2322 2455 2914 3123 727
Size: 291 1304 133 459 209 2343
Fragments arranged by size:
2343 1304 459 291 209 133
TatI w'GTAC_w
Cuts at: 344 1055 4254 344
Size: 711 3199 829
Fragments arranged by size:
3199 829 711
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4256 4288 4256
Size: 32 4707
Tth111I GACn'n_nGTC
Cuts at: 2536 2536
Size: 4739
XbaI T'CTAG_A
Cuts at: 1770 1770
Size: 4739
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 507 945 1299 1557 2234 507
Size: 438 354 258 677 3012
Fragments arranged by size:
3012 677 438 354 258
XmaI C'CCGG_G
Cuts at: 1759 1759
Size: 4739
XmnI GAAnn'nnTTC
Cuts at: 637 637
Size: 4739
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsiWI BsmBI BssHII BstEII BstZ17I
ClaI DraIII EcoRV FseI FspAI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI