Restriction map for
pF1KB4872
(Circular) (Six-base) MAPSORT of: pF1KB4872.seq Check: 3946 from: 1 to: 11945
pF1KB4872 11945 bp
With 182 enzymes: SgfI *
May 11, 2012 13:46 ..
AatII G_ACGT'C
Cuts at: 4650 4650
Size: 11945
AccI GT'mk_AC
Cuts at: 2353 5234 6567 8983 2353
Size: 2881 1333 2416 5315
Fragments arranged by size:
5315 2881 2416 1333
Acc65I G'GTAC_C
Cuts at: 8640 8783 8961 8640
Size: 143 178 11624
Fragments arranged by size:
11624 178 143
AclI AA'CG_TT
Cuts at: 11785 11785
Size: 11945
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 676 693 4688 6139 6617 6742 6794 8305
Size: 60 17 3995 1451 478 125 52 1511
Cuts at: 8305 8726 9770 10202 10949 11217 616
Size: 421 1044 432 747 268 1344
Fragments arranged by size:
3995 1511 1451 1344 1044 747 478 432
421 268 125 60 52 17
AflIII A'CryG_T
Cuts at: 2994 3539 9297 10401 11378 2994
Size: 545 5758 1104 977 3561
Fragments arranged by size:
5758 3561 1104 977 545
AgeI A'CCGG_T
Cuts at: 1439 3946 10333 1439
Size: 2507 6387 3051
Fragments arranged by size:
6387 3051 2507
AhdI GACnn_n'nnGTC
Cuts at: 523 558 523
Size: 35 11910
AleI CACnn'nnGTG
Cuts at: 3517 3517
Size: 11945
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1744 3475 1744
Size: 1731 10214
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1094 1126 3259 3291 5546 5578 1094
Size: 32 2133 32 2255 32 7461
Fragments arranged by size:
7461 2255 2133 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 469 570 933 1224 1368 1702 2034 2347 2590
Size: 101 363 291 144 334 332 313 243
Cuts at: 2590 4984 5245 6409 8425 8575 8929 9250 10817
Size: 2394 261 1164 2016 150 354 321 1567
Cuts at: 10817 469
Size: 1597
Fragments arranged by size:
2394 2016 1597 1567 1164 363 354 334
332 321 313 291 261 243 150 144
101
ApaI G_GGCC'C
Cuts at: 1208 1231 1322 1390 2131 2170 2373 2396 2849
Size: 23 91 68 741 39 203 23 453
Cuts at: 2849 2939 3971 4231 4513 4568 4790 4799 4834
Size: 90 1032 260 282 55 222 9 35
Cuts at: 4834 5303 5483 5797 5839 6073 6091 6184 6365
Size: 469 180 314 42 234 18 93 181
Cuts at: 6365 6999 7517 7598 8124 1208
Size: 634 518 81 526 5029
Fragments arranged by size:
5029 1032 741 634 526 518 469 453
314 282 260 234 222 203 181 180
93 91 90 81 68 55 42 39
35 23 23 18 9
ApaLI G'TGCA_C
Cuts at: 326 753 10715 326
Size: 427 9962 1556
Fragments arranged by size:
9962 1556 427
ApoI r'AATT_y
Cuts at: 8949 11298 11554 8949
Size: 2349 256 9340
Fragments arranged by size:
9340 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 11945
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11945
AvaI C'yCGr_G
Cuts at: 164 1666 1838 2834 2848 3936 4116 4133 4622
Size: 1502 172 996 14 1088 180 17 489
Cuts at: 4622 4994 5049 5220 5294 6187 6232 6561 6678
Size: 372 55 171 74 893 45 329 117
Cuts at: 6678 7233 7323 7340 7517 7580 7736 7763 7899
Size: 555 90 17 177 63 156 27 136
Cuts at: 7899 7970 8062 8435 8900 8965 164
Size: 71 92 373 465 65 3144
Fragments arranged by size:
3144 1502 1088 996 893 555 489 465
373 372 329 180 177 172 171 156
136 117 92 90 74 71 65 63
55 45 27 17 17 14
AvrII C'CTAG_G
Cuts at: 2916 2916
Size: 11945
BamHI G'GATC_C
Cuts at: 146 3467 4618 5863 7886 7903 8970 146
Size: 3321 1151 1245 2023 17 1067 3121
Fragments arranged by size:
3321 3121 2023 1245 1151 1067 17
BanI G'GyrC_C
Cuts at: 159 1162 1419 1705 2239 2561 2775 3546 3563
Size: 1003 257 286 534 322 214 771 17
Cuts at: 3563 3717 5091 5695 6673 6895 7705 8640 8783
Size: 154 1374 604 978 222 810 935 143
Cuts at: 8783 8961 9375 9623 9658 159
Size: 178 414 248 35 2446
Fragments arranged by size:
2446 1374 1003 978 935 810 771 604
534 414 322 286 257 248 222 214
178 154 143 35 17
BanII G_rGCy'C
Cuts at: 177 1208 1231 1322 1390 1636 1696 2131 2170
Size: 1031 23 91 68 246 60 435 39
Cuts at: 2170 2373 2396 2849 2855 2939 3845 3971 4071
Size: 203 23 453 6 84 906 126 100
Cuts at: 4071 4087 4161 4231 4470 4513 4568 4790 4799
Size: 16 74 70 239 43 55 222 9
Cuts at: 4799 4834 5303 5331 5483 5745 5797 5839 6007
Size: 35 469 28 152 262 52 42 168
Cuts at: 6007 6073 6091 6184 6239 6365 6882 6928 6999
Size: 66 18 93 55 126 517 46 71
Cuts at: 6999 7022 7050 7157 7191 7240 7517 7598 7825
Size: 23 28 107 34 49 277 81 227
Cuts at: 7825 8124 8373 8391 8758 8959 9989 177
Size: 299 249 18 367 201 1030 2133
Fragments arranged by size:
2133 1031 1030 906 517 469 453 435
367 299 277 262 249 246 239 227
222 203 201 168 152 126 126 107
100 93 91 84 81 74 71 70
68 66 60 55 55 52 49 46
43 42 39 35 34 28 28 23
23 23 18 18 16 9 6
BbeI G_GCGC'C
Cuts at: 163 2243 3567 3721 5699 9379 9627 163
Size: 2080 1324 154 1978 3680 248 2481
Fragments arranged by size:
3680 2481 2080 1978 1324 248 154
BbsI GAAGACnn'nnnn_
Cuts at: 489 4381 5669 489
Size: 3892 1288 6765
Fragments arranged by size:
6765 3892 1288
BbvCI CC'TCA_GC
Cuts at: 2755 2755
Size: 11945
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 270 304 3691 3725 270
Size: 34 3387 34 8490
Fragments arranged by size:
8490 3387 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2242 5761 7942 9843 10604 11945 2242
Size: 3519 2181 1901 761 1341 2242
Fragments arranged by size:
3519 2242 2181 1901 1341 761
BclI T'GATC_A
Cuts at: 545 9465 545
Size: 8920 3025
BglI GCCn_nnn'nGGC
Cuts at: 2317 2377 3257 3366 3938 4082 4421 4794 4838
Size: 60 880 109 572 144 339 373 44
Cuts at: 4838 4907 5487 5567 5801 6033 6077 6095 6635
Size: 69 580 80 234 232 44 18 540
Cuts at: 6635 6812 7289 7565 2317
Size: 177 477 276 6697
Fragments arranged by size:
6697 880 580 572 540 477 373 339
276 234 232 177 144 109 80 69
60 44 44 18
BglII A'GATC_T
Cuts at: 7275 9460 11497 7275
Size: 2185 2037 7723
Fragments arranged by size:
7723 2185 2037
BlpI GC'TnA_GC
Cuts at: 436 1813 2321 2854 7824 9057 436
Size: 1377 508 533 4970 1233 3324
Fragments arranged by size:
4970 3324 1377 1233 533 508
Bme1580I G_kGCm'C
Cuts at: 330 757 1208 1231 1322 1390 1540 2131 2170
Size: 427 451 23 91 68 150 591 39
Cuts at: 2170 2373 2396 2668 2780 2849 2939 3253 3514
Size: 203 23 272 112 69 90 314 261
Cuts at: 3514 3971 4231 4513 4568 4790 4799 4834 5094
Size: 457 260 282 55 222 9 35 260
Cuts at: 5094 5303 5483 5797 5839 6029 6073 6091 6184
Size: 209 180 314 42 190 44 18 93
Cuts at: 6184 6365 6678 6999 7517 7598 8124 9570 9663
Size: 181 313 321 518 81 526 1446 93
Cuts at: 9663 10719 330
Size: 1056 1556
Fragments arranged by size:
1556 1446 1056 591 526 518 457 451
427 321 314 314 313 282 272 261
260 260 222 209 203 190 181 180
150 112 93 93 91 90 81 69
68 55 44 42 39 35 23 23
18 9
BmrI ACTGGGnnnn_n'
Cuts at: 1690 1707 2164 2173 2286 3844 4261 6634 8243
Size: 17 457 9 113 1558 417 2373 1609
Cuts at: 8243 9157 9238 9573 11717 1690
Size: 914 81 335 2144 1918
Fragments arranged by size:
2373 2144 1918 1609 1558 914 457 417
335 113 81 17 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10312 10312
Size: 11945
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 1591 1710 1994 2019 2356 2528 2899 2948
Size: 1256 119 284 25 337 172 371 49
Cuts at: 2948 3023 3355 3707 3905 4031 4073 4085 4454
Size: 75 332 352 198 126 42 12 369
Cuts at: 4454 4547 4601 4673 4874 4901 4964 5117 5162
Size: 93 54 72 201 27 63 153 45
Cuts at: 5162 5267 5345 5678 5756 5909 6125 6440 6734
Size: 105 78 333 78 153 216 315 294
Cuts at: 6734 6755 6797 6821 7046 7073 7085 7175 7244
Size: 21 42 24 225 27 12 90 69
Cuts at: 7244 7517 7676 8405 335
Size: 273 159 729 3875
Fragments arranged by size:
3875 1256 729 371 369 352 337 333
332 315 294 284 273 225 216 201
198 172 159 153 153 126 119 105
93 90 78 78 75 72 69 63
54 49 45 42 42 27 27 25
24 21 12 12
Bpu10I CC'TnA_GC
Cuts at: 2426 2755 6452 2426
Size: 329 3697 7919
Fragments arranged by size:
7919 3697 329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 738 870 3120 3278 3633 6427 7963 9121 10000
Size: 132 2250 158 355 2794 1536 1158 879
Cuts at: 10000 10492 10790 738
Size: 492 298 1893
Fragments arranged by size:
2794 2250 1893 1536 1158 879 492 355
298 158 132
BsaI GGTCTCn'nnnn_
Cuts at: 809 967 1000 2611 4499 4850 4868 4877 4949
Size: 158 33 1611 1888 351 18 9 72
Cuts at: 4949 5120 5983 6128 6515 6650 6839 7061 7154
Size: 171 863 145 387 135 189 222 93
Cuts at: 7154 7484 11649 809
Size: 330 4165 1105
Fragments arranged by size:
4165 1888 1611 1105 863 387 351 330
222 189 171 158 145 135 93 72
33 18 9
BsaAI yAC'GTr
Cuts at: 2730 2995 3576 9298 2730
Size: 265 581 5722 5377
Fragments arranged by size:
5722 5377 581 265
BsaBI GATnn'nnATC
Cuts at: 6 9459 11496 6
Size: 9453 2037 455
Fragments arranged by size:
9453 2037 455
BsaHI Gr'CG_yC
Cuts at: 160 2240 3170 3193 3241 3564 3718 4023 4647
Size: 2080 930 23 48 323 154 305 624
Cuts at: 4647 5696 6036 9376 9624 10326 11822 160
Size: 1049 340 3340 248 702 1496 283
Fragments arranged by size:
3340 2080 1496 1049 930 702 624 340
323 305 283 248 154 48 23
BsaWI w'CCGG_w
Cuts at: 789 1439 2016 3946 4810 9134 9655 10333 10607
Size: 650 577 1930 864 4324 521 678 274
Cuts at: 10607 10754 11165 789
Size: 147 411 1569
Fragments arranged by size:
4324 1930 1569 864 678 650 577 521
411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 771 792 801 822 3169 3199 3936 3966 5546
Size: 21 9 21 2347 30 737 30 1580
Cuts at: 5546 5576 5697 5727 6732 6762 6915 6945 7058
Size: 30 121 30 1005 30 153 30 113
Cuts at: 7058 7088 7214 7244 8345 8375 8537 8567 771
Size: 30 126 30 1101 30 162 30 4149
Fragments arranged by size:
4149 2347 1580 1101 1005 737 162 153
126 121 113 30 30 30 30 30
30 30 30 30 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 234 664 899 2137 3339 4817 5921 7358 7952
Size: 430 235 1238 1202 1478 1104 1437 594
Cuts at: 7952 8641 8704 234
Size: 689 63 3475
Fragments arranged by size:
3475 1478 1437 1238 1202 1104 689 594
430 235 63
BseYI C'CCAG_C
Cuts at: 176 506 921 963 1224 1807 2765 3143 4611
Size: 330 415 42 261 583 958 378 1468
Cuts at: 4611 4792 5400 5476 5512 6250 6589 8585 9370
Size: 181 608 76 36 738 339 1996 785
Cuts at: 9370 10128 10705 11201 176
Size: 758 577 496 920
Fragments arranged by size:
1996 1468 958 920 785 758 738 608
583 577 496 415 378 339 330 261
181 76 42 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 373 2627 2755 2785 7166 7664 7898 8441
Size: 190 2254 128 30 4381 498 234 543
Cuts at: 8441 8745 183
Size: 304 3383
Fragments arranged by size:
4381 3383 2254 543 498 304 234 190
128 30
BsiEI CG_ry'CG
Cuts at: 104 3014 4320 6129 9487 9533 10741 104
Size: 2910 1306 1809 3358 46 1208 1308
Fragments arranged by size:
3358 2910 1809 1308 1306 1208 46
BsiHKAI G_wGCw'C
Cuts at: 330 757 1297 2005 2750 2855 3954 4470 5745
Size: 427 540 708 745 105 1099 516 1275
Cuts at: 5745 6007 7305 8391 8609 8959 9737 9927 10719
Size: 262 1298 1086 218 350 778 190 792
Cuts at: 10719 330
Size: 1556
Fragments arranged by size:
1556 1298 1275 1099 1086 792 778 745
708 540 516 427 350 262 218 190
105
BsmI GAATG_Cn'
Cuts at: 642 1783 8856 642
Size: 1141 7073 3731
Fragments arranged by size:
7073 3731 1141
BsmBI CGTCTCn'nnnn_
Cuts at: 3177 5522 8619 3177
Size: 2345 3097 6503
Fragments arranged by size:
6503 3097 2345
Bsp1286I G_dGCh'C
Cuts at: 177 330 757 1208 1231 1297 1322 1390 1540
Size: 153 427 451 23 66 25 68 150
Cuts at: 1540 1636 1696 2005 2131 2170 2373 2396 2668
Size: 96 60 309 126 39 203 23 272
Cuts at: 2668 2750 2780 2849 2855 2939 3253 3514 3845
Size: 82 30 69 6 84 314 261 331
Cuts at: 3845 3954 3971 4071 4087 4161 4231 4470 4513
Size: 109 17 100 16 74 70 239 43
Cuts at: 4513 4568 4790 4799 4834 5094 5303 5331 5483
Size: 55 222 9 35 260 209 28 152
Cuts at: 5483 5745 5797 5839 6007 6029 6073 6091 6184
Size: 262 52 42 168 22 44 18 93
Cuts at: 6184 6239 6365 6678 6882 6928 6999 7022 7050
Size: 55 126 313 204 46 71 23 28
Cuts at: 7050 7157 7191 7240 7305 7517 7598 7825 8124
Size: 107 34 49 65 212 81 227 299
Cuts at: 8124 8373 8391 8609 8758 8959 9570 9663 9737
Size: 249 18 218 149 201 611 93 74
Cuts at: 9737 9927 9989 10719 177
Size: 190 62 730 1403
Fragments arranged by size:
1403 730 611 451 427 331 314 313
309 299 272 262 261 260 249 239
227 222 218 212 209 204 203 201
190 168 153 152 150 149 126 126
109 107 100 96 93 93 84 82
81 74 74 71 70 69 68 66
65 62 60 55 55 52 49 46
44 43 42 39 35 34 30 28
28 25 23 23 23 22 18 18
17 16 9 6
BspEI T'CCGG_A
Cuts at: 789 2016 11165 789
Size: 1227 9149 1569
Fragments arranged by size:
9149 1569 1227
BspHI T'CATG_A
Cuts at: 11121 11121
Size: 11945
BspMI ACCTGCnnnn'nnnn_
Cuts at: 205 1655 4755 8995 9209 9511 9892 205
Size: 1450 3100 4240 214 302 381 2258
Fragments arranged by size:
4240 3100 2258 1450 381 302 214
BsrBI CCG'CTC
Cuts at: 50 945 1067 1752 3691 4329 5450 7377 8214
Size: 895 122 685 1939 638 1121 1927 837
Cuts at: 8214 8637 10237 10291 11775 11940 50
Size: 423 1600 54 1484 165 55
Fragments arranged by size:
1939 1927 1600 1484 1121 895 837 685
638 423 165 122 55 54
BsrDI GCAATG_nn'
Cuts at: 4358 6477 9857 4358
Size: 2119 3380 6446
Fragments arranged by size:
6446 3380 2119
BsrFI r'CCGG_y
Cuts at: 1439 2772 3946 8428 8578 9943 10124 10333 1439
Size: 1333 1174 4482 150 1365 181 209 3051
Fragments arranged by size:
4482 3051 1365 1333 1174 209 181 150
BssHII G'CGCG_C
Cuts at: 2956 2956
Size: 11945
BssSI C'ACGA_G
Cuts at: 812 1579 2972 4106 4241 10216 10574 812
Size: 767 1393 1134 135 5975 358 2183
Fragments arranged by size:
5975 2183 1393 1134 767 358 135
BstAPI GCAn_nnn'nTGC
Cuts at: 325 1368 1677 3553 8575 9322 325
Size: 1043 309 1876 5022 747 2948
Fragments arranged by size:
5022 2948 1876 1043 747 309
BstBI TT'CG_AA
Cuts at: 10306 10306
Size: 11945
BstEII G'GTnAC_C
Cuts at: 1284 4546 5044 5188 5416 5845 6475 6941 7096
Size: 3262 498 144 228 429 630 466 155
Cuts at: 7096 7213 7894 8155 8191 1284
Size: 117 681 261 36 5038
Fragments arranged by size:
5038 3262 681 630 498 466 429 261
228 155 144 117 36
BstXI CCAn_nnnn'nTGG
Cuts at: 1522 2380 3448 3655 1522
Size: 858 1068 207 9812
Fragments arranged by size:
9812 1068 858 207
BstYI r'GATC_y
Cuts at: 1 146 2456 2597 3131 3467 4618 5136 5863
Size: 145 2310 141 534 336 1151 518 727
Cuts at: 5863 7275 7886 7903 8970 9437 9460 9795 10041
Size: 1412 611 17 1067 467 23 335 246
Cuts at: 10041 11053 11139 11151 11497 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2310 1412 1151 1067 1012 727 611 534
518 467 449 346 336 335 246 145
141 86 23 17 12
BstZ17I GTA'TAC
Cuts at: 2354 2354
Size: 11945
Bsu36I CC'TnA_GG
Cuts at: 647 1424 2699 2813 3221 6146 8648 647
Size: 777 1275 114 408 2925 2502 3944
Fragments arranged by size:
3944 2925 2502 1275 777 408 114
BtgI C'CryG_G
Cuts at: 370 1431 1601 2274 2312 3255 3518 3752 4269
Size: 1061 170 673 38 943 263 234 517
Cuts at: 4269 4513 5193 5277 5547 5850 6129 6989 7699
Size: 244 680 84 270 303 279 860 710
Cuts at: 7699 370
Size: 4616
Fragments arranged by size:
4616 1061 943 860 710 680 673 517
303 279 270 263 244 234 170 84
38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 377 8517 9895 10076 10239 377
Size: 8140 1378 181 163 2083
Fragments arranged by size:
8140 2083 1378 181 163
BtsI GCAGTG_nn'
Cuts at: 931 1082 1317 1866 2016 2032 3983 4742 8298
Size: 151 235 549 150 16 1951 759 3556
Cuts at: 8298 8573 8762 9188 931
Size: 275 189 426 3688
Fragments arranged by size:
3688 3556 1951 759 549 426 275 235
189 151 150 16
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4823 4858 4823
Size: 35 11910
DraI TTT'AAA
Cuts at: 8944 8944
Size: 11945
DraIII CAC_nnn'GTG
Cuts at: 334 1584 2733 2974 6673 7705 334
Size: 1250 1149 241 3699 1032 4574
Fragments arranged by size:
4574 3699 1250 1149 1032 241
DrdI GACnn_nn'nnGTC
Cuts at: 3042 3781 5288 5389 6545 7222 7865 9651 10509
Size: 739 1507 101 1156 677 643 1786 858
Cuts at: 10509 3042
Size: 4478
Fragments arranged by size:
4478 1786 1507 1156 858 739 677 643
101
EaeI y'GGCC_r
Cuts at: 128 255 869 1000 1305 1586 2820 3076 3093
Size: 127 614 131 305 281 1234 256 17
Cuts at: 3093 3794 3835 4038 4689 5442 5802 7140 7557
Size: 701 41 203 651 753 360 1338 417
Cuts at: 7557 7566 8416 9530 9704 10095 10122 11467 128
Size: 9 850 1114 174 391 27 1345 606
Fragments arranged by size:
1345 1338 1234 1114 850 753 701 651
614 606 417 391 360 305 281 256
203 174 131 127 41 27 17 9
EagI C'GGCC_G
Cuts at: 9530 9530
Size: 11945
EarI CTCTTCn'nnn_
Cuts at: 8627 9968 10178 8627
Size: 1341 210 10394
Fragments arranged by size:
10394 1341 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1164 1870 2696 3280 4218 10153 10463 10609 11755
Size: 706 826 584 938 5935 310 146 1146
Cuts at: 11755 1164
Size: 1354
Fragments arranged by size:
5935 1354 1146 938 826 706 584 310
146
EcoICRI GAG'CTC
Cuts at: 2853 4468 5743 6005 8389 8957 2853
Size: 1615 1275 262 2384 568 5841
Fragments arranged by size:
5841 2384 1615 1275 568 262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 616 676 693 1591 1710 1994 2019 2356
Size: 281 60 17 898 119 284 25 337
Cuts at: 2356 2528 2899 2948 3023 3355 3707 3905 4031
Size: 172 371 49 75 332 352 198 126
Cuts at: 4031 4073 4085 4454 4547 4601 4673 4688 4874
Size: 42 12 369 93 54 72 15 186
Cuts at: 4874 4901 4964 5117 5162 5267 5345 5678 5756
Size: 27 63 153 45 105 78 333 78
Cuts at: 5756 5909 6125 6139 6440 6617 6734 6742 6755
Size: 153 216 14 301 177 117 8 13
Cuts at: 6755 6794 6797 6821 7046 7073 7085 7175 7244
Size: 39 3 24 225 27 12 90 69
Cuts at: 7244 7517 7676 8305 8405 8726 9770 10202 10949
Size: 273 159 629 100 321 1044 432 747
Cuts at: 10949 11217 335
Size: 268 1063
Fragments arranged by size:
1063 1044 898 747 629 432 371 369
352 337 333 332 321 301 284 281
273 268 225 216 198 186 177 172
159 153 153 126 119 117 105 100
93 90 78 78 75 72 69 63
60 54 49 45 42 39 27 27
25 24 17 15 14 13 12 12
8 3
EcoNI CCTnn'n_nnAGG
Cuts at: 154 2226 3775 3808 4343 4573 7499 154
Size: 2072 1549 33 535 230 2926 4600
Fragments arranged by size:
4600 2926 2072 1549 535 230 33
EcoO109I rG'GnC_Cy
Cuts at: 567 1191 1228 1318 1387 1846 1963 2127 2128
Size: 624 37 90 69 459 117 164 1
Cuts at: 2128 2369 2393 2838 2845 2935 2936 3088 3110
Size: 241 24 445 7 90 1 152 22
Cuts at: 3110 3217 3330 3967 3968 4058 4113 4129 4186
Size: 107 113 637 1 90 55 16 57
Cuts at: 4186 4212 4228 4257 4264 4333 4414 4509 4519
Size: 26 16 29 7 69 81 95 10
Cuts at: 4519 4564 4565 4663 4681 4728 4744 4786 4830
Size: 45 1 98 18 47 16 42 44
Cuts at: 4830 4891 4918 4936 4981 5035 5107 5206 5215
Size: 61 27 18 45 54 72 99 9
Cuts at: 5215 5242 5290 5299 5362 5382 5425 5480 5517
Size: 27 48 9 63 20 43 55 37
Cuts at: 5517 5559 5652 5767 5794 5836 5880 6043 6069
Size: 42 93 115 27 42 44 163 26
Cuts at: 6069 6070 6087 6088 6180 6181 6362 6406 6430
Size: 1 17 1 92 1 181 44 24
Cuts at: 6430 6484 6628 6700 6709 6745 6759 6995 6996
Size: 54 144 72 9 36 14 236 1
Cuts at: 6996 7195 7230 7336 7363 7390 7417 7463 7514
Size: 199 35 106 27 27 27 46 51
Cuts at: 7514 7576 7594 7595 7687 7732 7759 7779 7858
Size: 62 18 1 92 45 27 20 79
Cuts at: 7858 7966 7974 8120 8121 8136 8182 8245 8308
Size: 108 8 146 1 15 46 63 63
Cuts at: 8308 8344 8379 8422 8700 8867 9084 567
Size: 36 35 43 278 167 217 3428
Fragments arranged by size:
3428 637 624 459 445 278 241 236
217 199 181 167 164 163 152 146
144 117 115 113 108 107 106 99
98 95 93 92 92 90 90 90
81 79 72 72 69 69 63 63
63 62 61 57 55 55 54 54
51 48 47 46 46 45 45 45
44 44 44 43 43 42 42 42
37 37 36 36 35 35 29 27
27 27 27 27 27 27 26 26
24 24 22 20 20 18 18 18
17 16 16 16 15 14 10 9
9 9 8 7 7 1 1 1
1 1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 8949 8949
Size: 11945
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 9964 9964
Size: 11945
FspI TGC'GCA
Cuts at: 1732 9726 1732
Size: 7994 3951
HaeII r_GCGC'y
Cuts at: 163 2243 3567 3721 5699 8889 9379 9627 10649
Size: 2080 1324 154 1978 3190 490 248 1022
Cuts at: 10649 11628 163
Size: 979 480
Fragments arranged by size:
3190 2080 1978 1324 1022 979 490 480
248 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 229 261 509 541 544 576 771 787 792
Size: 32 248 32 3 32 195 16 5
Cuts at: 792 803 819 824 2589 2621 3936 3968 4157
Size: 11 16 5 1765 32 1315 32 189
Cuts at: 4157 4189 5697 5729 5848 5880 5989 6021 6732
Size: 32 1508 32 119 32 109 32 711
Cuts at: 6732 6764 7214 7246 7323 7355 7394 7426 7807
Size: 32 450 32 77 32 39 32 381
Cuts at: 7807 7839 7888 7920 8647 8679 8740 8772 9787
Size: 32 49 32 727 32 61 32 1015
Cuts at: 9787 9817 9819 9849 229
Size: 30 2 30 2325
Fragments arranged by size:
2325 1765 1508 1315 1015 727 711 450
381 248 195 189 119 109 77 61
49 39 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 30 30 16 16 11
5 5 3 2
HincII GTy'rAC
Cuts at: 7870 8984 7870
Size: 1114 10831
KasI G'GCGC_C
Cuts at: 159 2239 3563 3717 5695 9375 9623 159
Size: 2080 1324 154 1978 3680 248 2481
Fragments arranged by size:
3680 2481 2080 1978 1324 248 154
KpnI G_GTAC'C
Cuts at: 8644 8787 8965 8644
Size: 143 178 11624
Fragments arranged by size:
11624 178 143
MluI A'CGCG_T
Cuts at: 11378 11378
Size: 11945
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1424 1499 4995 6945 10616 10800 1424
Size: 75 3496 1950 3671 184 2569
Fragments arranged by size:
3671 3496 2569 1950 184 75
MscI TGG'CCA
Cuts at: 1588 2822 3095 3796 5804 9706 1588
Size: 1234 273 701 2008 3902 3827
Fragments arranged by size:
3902 3827 2008 1234 701 273
MslI CAynn'nnrTG
Cuts at: 1726 3517 9934 10061 1726
Size: 1791 6417 127 3610
Fragments arranged by size:
6417 3610 1791 127
MspA1I CmG'CkG
Cuts at: 217 510 583 868 967 1674 2926 3147 3705
Size: 293 73 285 99 707 1252 221 558
Cuts at: 3705 4615 4792 5279 5476 7675 8891 9056 9370
Size: 910 177 487 197 2199 1216 165 314
Cuts at: 9370 9730 10743 10988 217
Size: 360 1013 245 1174
Fragments arranged by size:
2199 1252 1216 1174 1013 910 707 558
487 360 314 293 285 245 221 197
177 165 99 73
NaeI GCC'GGC
Cuts at: 8580 10126 8580
Size: 1546 10399
NarI GG'CG_CC
Cuts at: 160 2240 3564 3718 5696 9376 9624 160
Size: 2080 1324 154 1978 3680 248 2481
Fragments arranged by size:
3680 2481 2080 1978 1324 248 154
NgoMIV G'CCGG_C
Cuts at: 8578 10124 8578
Size: 1546 10399
NspI r_CATG'y
Cuts at: 2365 3289 3480 3543 8998 9498 10405 2365
Size: 924 191 63 5455 500 907 3905
Fragments arranged by size:
5455 3905 924 907 500 191 63
PasI CC'CwG_GG
Cuts at: 1231 1740 2715 3963 4279 4569 4723 4960 5332
Size: 509 975 1248 316 290 154 237 372
Cuts at: 5332 5431 5521 5763 5782 6358 7165 7243 7396
Size: 99 90 242 19 576 807 78 153
Cuts at: 7396 7422 7863 8278 1231
Size: 26 441 415 4898
Fragments arranged by size:
4898 1248 975 807 576 509 441 415
372 316 290 242 237 154 153 99
90 78 26 19
PciI A'CATG_T
Cuts at: 3539 10401 3539
Size: 6862 5083
PflMI CCAn_nnn'nTGG
Cuts at: 570 1584 1702 2222 3433 6409 6646 8230 570
Size: 1014 118 520 1211 2976 237 1584 4285
Fragments arranged by size:
4285 2976 1584 1211 1014 520 237 118
PfoI T'CCnGG_A
Cuts at: 742 1408 1687 3200 3332 4161 4287 4392 4524
Size: 666 279 1513 132 829 126 105 132
Cuts at: 4524 4650 4851 4941 5139 6102 6339 6408 6750
Size: 126 201 90 198 963 237 69 342
Cuts at: 6750 6792 7191 742
Size: 42 399 5496
Fragments arranged by size:
5496 1513 963 829 666 399 342 279
237 201 198 132 132 126 126 105
90 69 42
PmeI GTTT'AAAC
Cuts at: 8944 8944
Size: 11945
PmlI CAC'GTG
Cuts at: 2730 2730
Size: 11945
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5546 5578 11084 11116 5546
Size: 32 5506 32 6375
Fragments arranged by size:
6375 5506 32 32
PpuMI rG'GwC_Cy
Cuts at: 567 1191 1846 1963 2838 3110 3217 3330 4058
Size: 624 655 117 875 272 107 113 728
Cuts at: 4058 4113 4129 4186 4212 4257 4264 4333 4519
Size: 55 16 57 26 45 7 69 186
Cuts at: 4519 4663 4681 4728 4744 4891 4918 4981 5035
Size: 144 18 47 16 147 27 63 54
Cuts at: 5035 5206 5215 5242 5290 5362 5382 5425 5517
Size: 171 9 27 48 72 20 43 92
Cuts at: 5517 5652 6406 6430 6484 6700 6709 6745 7195
Size: 135 754 24 54 216 9 36 450
Cuts at: 7195 7230 7417 7576 7759 7779 7858 7966 8344
Size: 35 187 159 183 20 79 108 378
Cuts at: 8344 8379 8700 8867 567
Size: 35 321 167 3645
Fragments arranged by size:
3645 875 754 728 655 624 450 378
321 272 216 187 186 183 171 167
159 147 144 135 117 113 108 107
92 79 72 69 63 57 55 54
54 48 47 45 43 36 35 35
27 27 26 24 20 20 18 16
16 9 9 7
PshAI GACnn'nnGTC
Cuts at: 3196 4889 5033 5423 6482 3196
Size: 1693 144 390 1059 8659
Fragments arranged by size:
8659 1693 1059 390 144
PspOMI G'GGCC_C
Cuts at: 1204 1227 1318 1386 2127 2166 2369 2392 2845
Size: 23 91 68 741 39 203 23 453
Cuts at: 2845 2935 3967 4227 4509 4564 4786 4795 4830
Size: 90 1032 260 282 55 222 9 35
Cuts at: 4830 5299 5479 5793 5835 6069 6087 6180 6361
Size: 469 180 314 42 234 18 93 181
Cuts at: 6361 6995 7513 7594 8120 1204
Size: 634 518 81 526 5029
Fragments arranged by size:
5029 1032 741 634 526 518 469 453
314 282 260 234 222 203 181 180
93 91 90 81 68 55 42 39
35 23 23 18 9
PspXI vC'TCGA_Gb
Cuts at: 1838 4133 5294 7340 8435 1838
Size: 2295 1161 2046 1095 5348
Fragments arranged by size:
5348 2295 2046 1161 1095
PstI C_TGCA'G
Cuts at: 1532 2075 2139 2456 2595 2946 4737 5505 6617
Size: 543 64 317 139 351 1791 768 1112
Cuts at: 6617 6699 8571 8992 9677 1532
Size: 82 1872 421 685 3800
Fragments arranged by size:
3800 1872 1791 1112 768 685 543 421
351 317 139 82 64
PvuI CG_AT'CG
Cuts at: 104 3014 4320 104
Size: 2910 1306 7729
Fragments arranged by size:
7729 2910 1306
PvuII CAG'CTG
Cuts at: 510 583 967 2926 3705 7675 9370 9730 510
Size: 73 384 1959 779 3970 1695 360 2725
Fragments arranged by size:
3970 2725 1959 1695 779 384 360 73
RsrII CG'GwC_CG
Cuts at: 10140 10140
Size: 11945
SacI G_AGCT'C
Cuts at: 2855 4470 5745 6007 8391 8959 2855
Size: 1615 1275 262 2384 568 5841
Fragments arranged by size:
5841 2384 1615 1275 568 262
SacII CC_GC'GG
Cuts at: 5280 5280
Size: 11945
SalI G'TCGA_C
Cuts at: 8982 8982
Size: 11945
SanDI GG'GwC_CC
Cuts at: 1846 1963 3110 4058 4113 4212 4257 4333 4728
Size: 117 1147 948 55 99 45 76 395
Cuts at: 4728 4891 5206 5362 5382 5425 5517 5652 6430
Size: 163 315 156 20 43 92 135 778
Cuts at: 6430 7230 7576 7779 7858 7966 8379 8867 1846
Size: 800 346 203 79 108 413 488 4924
Fragments arranged by size:
4924 1147 948 800 778 488 413 395
346 315 203 163 156 135 117 108
99 92 79 76 55 45 43 20
SapI GCTCTTCn'nnn_
Cuts at: 8627 9968 10178 8627
Size: 1341 210 10394
Fragments arranged by size:
10394 1341 210
SbfI CC_TGCA'GG
Cuts at: 6699 8992 6699
Size: 2293 9652
ScaI AGT'ACT
Cuts at: 8662 11462 8662
Size: 2800 9145
SexAI A'CCwGG_T
Cuts at: 519 569 818 2090 4344 6156 6669 6945 7070
Size: 50 249 1272 2254 1812 513 276 125
Cuts at: 7070 7872 519
Size: 802 4592
Fragments arranged by size:
4592 2254 1812 1272 802 513 276 249
125 50
SfcI C'TryA_G
Cuts at: 33 1528 2071 2135 2452 2591 2942 4733 5501
Size: 1495 543 64 317 139 351 1791 768
Cuts at: 5501 6613 6695 8567 8988 9673 10666 10857 33
Size: 1112 82 1872 421 685 993 191 1121
Fragments arranged by size:
1872 1791 1495 1121 1112 993 768 685
543 421 351 317 191 139 82 64
SfiI GGCCn_nnn'nGGCC
Cuts at: 3366 4421 4794 5487 5567 5801 6635 6812 7289
Size: 1055 373 693 80 234 834 177 477
Cuts at: 7289 7565 3366
Size: 276 7746
Fragments arranged by size:
7746 1055 834 693 477 373 276 234
177 80
SfoI GGC'GCC
Cuts at: 161 2241 3565 3719 5697 9377 9625 161
Size: 2080 1324 154 1978 3680 248 2481
Fragments arranged by size:
3680 2481 2080 1978 1324 248 154
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11945
SmaI CCC'GGG
Cuts at: 1668 2836 3938 4118 4624 5051 5222 6189 6234
Size: 1168 1102 180 506 427 171 967 45
Cuts at: 6234 6563 6680 7235 7325 7901 7972 8064 8902
Size: 329 117 555 90 576 71 92 838
Cuts at: 8902 8967 1668
Size: 65 4646
Fragments arranged by size:
4646 1168 1102 967 838 576 555 506
427 329 180 171 117 92 90 71
65 45
SmlI C'TyrA_G
Cuts at: 717 849 1838 3135 3293 3612 4133 5294 6442
Size: 132 989 1297 158 319 521 1161 1148
Cuts at: 6442 7340 7978 8435 9100 10015 10507 10769 717
Size: 898 638 457 665 915 492 262 1893
Fragments arranged by size:
1893 1297 1161 1148 989 915 898 665
638 521 492 457 319 262 158 132
SpeI A'CTAG_T
Cuts at: 67 67
Size: 11945
SphI G_CATG'C
Cuts at: 8998 9498 8998
Size: 500 11445
SrfI GCCC'GGGC
Cuts at: 1668 1668
Size: 11945
StuI AGG'CCT
Cuts at: 4475 5543 5777 6815 7058 8815 8878 4475
Size: 1068 234 1038 243 1757 63 7542
Fragments arranged by size:
7542 1757 1068 1038 243 234 63
StyI C'CwwG_G
Cuts at: 530 2916 3447 3989 4410 4658 4695 4818 4868
Size: 2386 531 542 421 248 37 123 50
Cuts at: 4868 5183 5303 5726 5754 5840 6047 6168 6383
Size: 315 120 423 28 86 207 121 215
Cuts at: 6383 6480 6705 7709 8150 8186 8713 9079 9433
Size: 97 225 1004 441 36 527 366 354
Cuts at: 9433 530
Size: 3042
Fragments arranged by size:
3042 2386 1004 542 531 527 441 423
421 366 354 315 248 225 215 207
123 121 120 97 86 50 37 36
28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1024 1726 1753 4561 5104 6555 6634 7066 7115
Size: 702 27 2808 543 1451 79 432 49
Cuts at: 7115 7711 8134 8174 9528 9661 10120 10329 1024
Size: 596 423 40 1354 133 459 209 2640
Fragments arranged by size:
2808 2640 1451 1354 702 596 543 459
432 423 209 133 79 49 40 27
TatI w'GTAC_w
Cuts at: 356 855 902 8660 11460 356
Size: 499 47 7758 2800 841
Fragments arranged by size:
7758 2800 841 499 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6915 6947 6995 7027 7058 7090 7878 7910 8345
Size: 32 48 32 31 32 788 32 435
Cuts at: 8345 8377 8537 8569 11462 11494 6915
Size: 32 160 32 2893 32 7366
Fragments arranged by size:
7366 2893 788 435 160 48 32 32
32 32 32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 493 3733 9742 493
Size: 3240 6009 2696
Fragments arranged by size:
6009 3240 2696
XbaI T'CTAG_A
Cuts at: 8976 8976
Size: 11945
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1332 2091 2299 3103 3784 3970 4417 5137 5812
Size: 759 208 804 681 186 447 720 675
Cuts at: 5812 6390 6712 7716 7870 9440 1332
Size: 578 322 1004 154 1570 3837
Fragments arranged by size:
3837 1570 1004 804 759 720 681 675
578 447 322 208 186 154
XhoI C'TCGA_G
Cuts at: 1838 4133 5294 7340 8435 1838
Size: 2295 1161 2046 1095 5348
Fragments arranged by size:
5348 2295 2046 1161 1095
XmaI C'CCGG_G
Cuts at: 1666 2834 3936 4116 4622 5049 5220 6187 6232
Size: 1168 1102 180 506 427 171 967 45
Cuts at: 6232 6561 6678 7233 7323 7899 7970 8062 8900
Size: 329 117 555 90 576 71 92 838
Cuts at: 8900 8965 1666
Size: 65 4646
Fragments arranged by size:
4646 1168 1102 967 838 576 555 506
427 329 180 171 117 92 90 71
65 45
XmnI GAAnn'nnTTC
Cuts at: 5354 8318 5354
Size: 2964 8981
ZraI GAC'GTC
Cuts at: 4648 4648
Size: 11945
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AscI BaeI BfrBI BmgBI BmtI
BsiWI BsrGI ClaI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PsiI PsrI SgrAI SnaBI SspI SwaI