Restriction map for
pF1KB4832
(Circular) (Six-base) MAPSORT of: pF1KB4832.seq Check: 243 from: 1 to: 4379
pF1KB4832 4379 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 281 740 281
Size: 459 3920
AccI GT'mk_AC
Cuts at: 281 1417 281
Size: 1136 3243
Acc65I G'GTAC_C
Cuts at: 1395 1395
Size: 4379
AclI AA'CG_TT
Cuts at: 4219 4219
Size: 4379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 975 2204 2636 3383 3651 673
Size: 302 1229 432 747 268 1401
Fragments arranged by size:
1401 1229 747 432 302 268
AflIII A'CryG_T
Cuts at: 1731 2835 3812 1731
Size: 1104 977 2298
Fragments arranged by size:
2298 1104 977
AgeI A'CCGG_T
Cuts at: 2767 2767
Size: 4379
AlwNI CAG_nnn'CTG
Cuts at: 509 1281 1353 1684 3251 509
Size: 772 72 331 1567 1637
Fragments arranged by size:
1637 1567 772 331 72
ApaLI G'TGCA_C
Cuts at: 3149 3149
Size: 4379
ApoI r'AATT_y
Cuts at: 706 1383 3732 3988 706
Size: 677 2349 256 1097
Fragments arranged by size:
2349 1097 677 256
AseI AT'TA_AT
Cuts at: 20 878 20
Size: 858 3521
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
AvaI C'yCGr_G
Cuts at: 1294 1399 1294
Size: 105 4274
BamHI G'GATC_C
Cuts at: 1058 1404 1058
Size: 346 4033
BanI G'GyrC_C
Cuts at: 589 611 1395 1809 2057 2092 589
Size: 22 784 414 248 35 2876
Fragments arranged by size:
2876 784 414 248 35 22
BanII G_rGCy'C
Cuts at: 134 814 1361 1393 2423 134
Size: 680 547 32 1030 2090
Fragments arranged by size:
2090 1030 680 547 32
BbeI G_GCGC'C
Cuts at: 1813 2061 1813
Size: 248 4131
BbsI GAAGACnn'nnnn_
Cuts at: 556 671 1187 556
Size: 115 516 3748
Fragments arranged by size:
3748 516 115
BciVI GTATCCnnnnn_n'
Cuts at: 2277 3038 4379 2277
Size: 761 1341 2277
Fragments arranged by size:
2277 1341 761
BclI T'GATC_A
Cuts at: 1899 1899
Size: 4379
BglI GCCn_nnn'nGGC
Cuts at: 773 773
Size: 4379
BglII A'GATC_T
Cuts at: 599 748 1894 3931 599
Size: 149 1146 2037 1047
Fragments arranged by size:
2037 1146 1047 149
BlpI GC'TnA_GC
Cuts at: 1491 1491
Size: 4379
Bme1580I G_kGCm'C
Cuts at: 592 616 1345 2004 2097 3153 592
Size: 24 729 659 93 1056 1818
Fragments arranged by size:
1818 1056 729 659 93 24
BmgBI CAC'GTC
Cuts at: 1071 1071
Size: 4379
BmrI ACTGGGnnnn_n'
Cuts at: 817 1591 1672 2007 4151 817
Size: 774 81 335 2144 1045
Fragments arranged by size:
2144 1045 774 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 645 2746 645
Size: 2101 2278
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 615 877 1375 615
Size: 262 498 3619
Fragments arranged by size:
3619 498 262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1555 2434 2926 3224 1555
Size: 879 492 298 2710
Fragments arranged by size:
2710 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4083 4083
Size: 4379
BsaAI yAC'GTr
Cuts at: 1732 1732
Size: 4379
BsaBI GATnn'nnATC
Cuts at: 6 1893 3930 6
Size: 1887 2037 455
Fragments arranged by size:
2037 1887 455
BsaHI Gr'CG_yC
Cuts at: 278 737 1810 2058 2760 4256 278
Size: 459 1073 248 702 1496 401
Fragments arranged by size:
1496 1073 702 459 401 248
BsaWI w'CCGG_w
Cuts at: 1568 2089 2767 3041 3188 3599 1568
Size: 521 678 274 147 411 2348
Fragments arranged by size:
2348 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 389 419 389
Size: 30 4349
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 655 966 655
Size: 311 4068
BseYI C'CCAG_C
Cuts at: 517 824 1218 1257 1804 2562 3139 3635 517
Size: 307 394 39 547 758 577 496 1261
Fragments arranged by size:
1261 758 577 547 496 394 307 39
BsiEI CG_ry'CG
Cuts at: 104 558 1921 1967 3175 104
Size: 454 1363 46 1208 1308
Fragments arranged by size:
1363 1308 1208 454 46
BsiHKAI G_wGCw'C
Cuts at: 496 1372 1393 2171 2361 3153 496
Size: 876 21 778 190 792 1722
Fragments arranged by size:
1722 876 792 778 190 21
BsmI GAATG_Cn'
Cuts at: 476 476
Size: 4379
BsmBI CGTCTCn'nnnn_
Cuts at: 1007 1007
Size: 4379
Bsp1286I G_dGCh'C
Cuts at: 134 496 592 616 814 1345 1361 1372 1393
Size: 362 96 24 198 531 16 11 21
Cuts at: 1393 2004 2097 2171 2361 2423 3153 134
Size: 611 93 74 190 62 730 1360
Fragments arranged by size:
1360 730 611 531 362 198 190 96
93 74 62 24 21 16 11
BspEI T'CCGG_A
Cuts at: 3599 3599
Size: 4379
BspHI T'CATG_A
Cuts at: 3555 3555
Size: 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1429 1643 1945 2326 1429
Size: 214 302 381 3482
Fragments arranged by size:
3482 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2671 2725 4209 4374 50
Size: 2621 54 1484 165 55
Fragments arranged by size:
2621 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 732 2291 732
Size: 1559 2820
BsrFI r'CCGG_y
Cuts at: 206 553 2377 2558 2767 206
Size: 347 1824 181 209 1818
Fragments arranged by size:
1824 1818 347 209 181
BssSI C'ACGA_G
Cuts at: 2650 3008 2650
Size: 358 4021
BstAPI GCAn_nnn'nTGC
Cuts at: 1281 1756 1281
Size: 475 3904
BstBI TT'CG_AA
Cuts at: 2740 2740
Size: 4379
BstEII G'GTnAC_C
Cuts at: 268 417 268
Size: 149 4230
BstYI r'GATC_y
Cuts at: 1 345 359 599 748 1058 1333 1404 1871
Size: 344 14 240 149 310 275 71 467
Cuts at: 1871 1894 2229 2475 3487 3573 3585 3931 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 344 335 310 275
246 240 149 86 71 23 14 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 159 2329 2510 2673 159
Size: 2170 181 163 1865
Fragments arranged by size:
2170 1865 181 163
BtsI GCAGTG_nn'
Cuts at: 1622 1622
Size: 4379
DraI TTT'AAA
Cuts at: 1378 1378
Size: 4379
DrdI GACnn_nn'nnGTC
Cuts at: 2085 2943 2085
Size: 858 3521
EaeI y'GGCC_r
Cuts at: 555 581 1964 2138 2529 2556 3901 555
Size: 26 1383 174 391 27 1345 1033
Fragments arranged by size:
1383 1345 1033 391 174 27 26
EagI C'GGCC_G
Cuts at: 555 1964 555
Size: 1409 2970
EarI CTCTTCn'nnn_
Cuts at: 2402 2612 2402
Size: 210 4169
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2587 2897 3043 4189 2587
Size: 310 146 1146 2777
Fragments arranged by size:
2777 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1391 1391
Size: 4379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 615 673 877 975 1375 2204 2636 3383 3651
Size: 58 204 98 400 829 432 747 268
Cuts at: 3651 615
Size: 1343
Fragments arranged by size:
1343 829 747 432 400 268 204 98
58
EcoNI CCTnn'n_nnAGG
Cuts at: 655 655
Size: 4379
EcoO109I rG'GnC_Cy
Cuts at: 1173 1518 1173
Size: 345 4034
EcoRI G'AATT_C
Cuts at: 1383 1383
Size: 4379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4379
FspI TGC'GCA
Cuts at: 2160 2160
Size: 4379
HaeII r_GCGC'y
Cuts at: 1813 2061 3083 4062 1813
Size: 248 1022 979 2130
Fragments arranged by size:
2130 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 389 421 1195 1227 2221 2251 2253 2283 389
Size: 32 774 32 994 30 2 30 2485
Fragments arranged by size:
2485 994 774 32 32 30 30 2
HincII GTy'rAC
Cuts at: 282 1418 282
Size: 1136 3243
KasI G'GCGC_C
Cuts at: 1809 2057 1809
Size: 248 4131
KpnI G_GTAC'C
Cuts at: 1399 1399
Size: 4379
MluI A'CGCG_T
Cuts at: 3812 3812
Size: 4379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 599 828 3050 3234 599
Size: 229 2222 184 1744
Fragments arranged by size:
2222 1744 229 184
MscI TGG'CCA
Cuts at: 583 2140 583
Size: 1557 2822
MslI CAynn'nnrTG
Cuts at: 1042 2368 2495 1042
Size: 1326 127 2926
Fragments arranged by size:
2926 1326 127
MspA1I CmG'CkG
Cuts at: 357 447 535 560 824 1490 1804 2164 3177
Size: 90 88 25 264 666 314 360 1013
Cuts at: 3177 3422 357
Size: 245 1314
Fragments arranged by size:
1314 1013 666 360 314 264 245 90
88 25
NaeI GCC'GGC
Cuts at: 2560 2560
Size: 4379
NarI GG'CG_CC
Cuts at: 1810 2058 1810
Size: 248 4131
NcoI C'CATG_G
Cuts at: 107 107
Size: 4379
NgoMIV G'CCGG_C
Cuts at: 2558 2558
Size: 4379
NspI r_CATG'y
Cuts at: 1432 1932 2839 1432
Size: 500 907 2972
Fragments arranged by size:
2972 907 500
PasI CC'CwG_GG
Cuts at: 393 393
Size: 4379
PciI A'CATG_T
Cuts at: 2835 2835
Size: 4379
PfoI T'CCnGG_A
Cuts at: 842 1352 842
Size: 510 3869
PmeI GTTT'AAAC
Cuts at: 1378 1378
Size: 4379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 3518
Size: 32 4347
PstI C_TGCA'G
Cuts at: 218 296 1286 1426 2111 218
Size: 78 990 140 685 2486
Fragments arranged by size:
2486 990 685 140 78
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4379
PvuII CAG'CTG
Cuts at: 535 1804 2164 535
Size: 1269 360 2750
Fragments arranged by size:
2750 1269 360
RsrII CG'GwC_CG
Cuts at: 2574 2574
Size: 4379
SacI G_AGCT'C
Cuts at: 1393 1393
Size: 4379
SalI G'TCGA_C
Cuts at: 280 1416 280
Size: 1136 3243
SapI GCTCTTCn'nnn_
Cuts at: 2402 2612 2402
Size: 210 4169
SbfI CC_TGCA'GG
Cuts at: 1426 1426
Size: 4379
ScaI AGT'ACT
Cuts at: 3896 3896
Size: 4379
SexAI A'CCwGG_T
Cuts at: 236 1106 236
Size: 870 3509
SfcI C'TryA_G
Cuts at: 33 214 292 1282 1422 2107 3100 3291 33
Size: 181 78 990 140 685 993 191 1121
Fragments arranged by size:
1121 993 990 685 191 181 140 78
SfoI GGC'GCC
Cuts at: 1811 2059 1811
Size: 248 4131
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
SmaI CCC'GGG
Cuts at: 1401 1401
Size: 4379
SmlI C'TyrA_G
Cuts at: 1534 2449 2941 3203 1534
Size: 915 492 262 2710
Fragments arranged by size:
2710 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4379
SphI G_CATG'C
Cuts at: 1432 1932 1432
Size: 500 3879
SspI AAT'ATT
Cuts at: 882 882
Size: 4379
StuI AGG'CCT
Cuts at: 220 220
Size: 4379
StyI C'CwwG_G
Cuts at: 107 497 1513 1867 107
Size: 390 1016 354 2619
Fragments arranged by size:
2619 1016 390 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 175 237 1962 2095 2554 2763 175
Size: 62 1725 133 459 209 1791
Fragments arranged by size:
1791 1725 459 209 133 62
TatI w'GTAC_w
Cuts at: 3894 3894
Size: 4379
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 530 562 3896 3928 530
Size: 32 3334 32 981
Fragments arranged by size:
3334 981 32 32
Tth111I GACn'n_nGTC
Cuts at: 379 2176 379
Size: 1797 2582
XbaI T'CTAG_A
Cuts at: 1410 1410
Size: 4379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 804 1874 804
Size: 1070 3309
XmaI C'CCGG_G
Cuts at: 1399 1399
Size: 4379
XmnI GAAnn'nnTTC
Cuts at: 476 476
Size: 4379
ZraI GAC'GTC
Cuts at: 279 738 279
Size: 459 3920
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I
BsgI BsiWI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SwaI XhoI