Restriction map for
pF1KB4828
(Circular) (Six-base) MAPSORT of: pF1KB4828.seq Check: 9737 from: 1 to: 3986
pF1KB4828 3986 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 553 553
Size: 3986
AccI GT'mk_AC
Cuts at: 170 1024 170
Size: 854 3132
Acc65I G'GTAC_C
Cuts at: 1002 1002
Size: 3986
AclI AA'CG_TT
Cuts at: 3826 3826
Size: 3986
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 971 1811 2243 2990 3258 535
Size: 436 840 432 747 268 1263
Fragments arranged by size:
1263 840 747 436 432 268
AflIII A'CryG_T
Cuts at: 607 1338 2442 3419 607
Size: 731 1104 977 1174
Fragments arranged by size:
1174 1104 977 731
AgeI A'CCGG_T
Cuts at: 2374 2374
Size: 3986
AleI CACnn'nnGTG
Cuts at: 610 610
Size: 3986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 136 136
Size: 3986
AlwNI CAG_nnn'CTG
Cuts at: 353 949 1291 2858 353
Size: 596 342 1567 1481
Fragments arranged by size:
1567 1481 596 342
ApaLI G'TGCA_C
Cuts at: 424 2756 424
Size: 2332 1654
ApoI r'AATT_y
Cuts at: 990 3339 3595 990
Size: 2349 256 1381
Fragments arranged by size:
2349 1381 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3986
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
AvaI C'yCGr_G
Cuts at: 1006 1006
Size: 3986
BamHI G'GATC_C
Cuts at: 1011 1011
Size: 3986
BanI G'GyrC_C
Cuts at: 1002 1416 1664 1699 1002
Size: 414 248 35 3289
Fragments arranged by size:
3289 414 248 35
BanII G_rGCy'C
Cuts at: 382 460 482 881 1000 2030 382
Size: 78 22 399 119 1030 2338
Fragments arranged by size:
2338 1030 399 119 78 22
BbeI G_GCGC'C
Cuts at: 1420 1668 1420
Size: 248 3738
BbsI GAAGACnn'nnnn_
Cuts at: 223 959 223
Size: 736 3250
BbvCI CC'TCA_GC
Cuts at: 650 650
Size: 3986
BciVI GTATCCnnnnn_n'
Cuts at: 1884 2645 3986 1884
Size: 761 1341 1884
Fragments arranged by size:
1884 1341 761
BclI T'GATC_A
Cuts at: 386 1506 386
Size: 1120 2866
BglII A'GATC_T
Cuts at: 128 1501 3538 128
Size: 1373 2037 576
Fragments arranged by size:
2037 1373 576
BlpI GC'TnA_GC
Cuts at: 1098 1098
Size: 3986
Bme1580I G_kGCm'C
Cuts at: 428 1611 1704 2760 428
Size: 1183 93 1056 1654
Fragments arranged by size:
1654 1183 1056 93
BmgBI CAC'GTC
Cuts at: 594 978 594
Size: 384 3602
BmrI ACTGGGnnnn_n'
Cuts at: 1198 1279 1614 3758 1198
Size: 81 335 2144 1426
Fragments arranged by size:
2144 1426 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 3986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 980 980
Size: 3986
Bpu10I CC'TnA_GC
Cuts at: 650 650
Size: 3986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 1162 2041 2533 2831 778
Size: 384 879 492 298 1933
Fragments arranged by size:
1933 879 492 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 499 3690 499
Size: 3191 795
BsaAI yAC'GTr
Cuts at: 1339 1339
Size: 3986
BsaBI GATnn'nnATC
Cuts at: 6 1500 3537 6
Size: 1494 2037 455
Fragments arranged by size:
2037 1494 455
BsaHI Gr'CG_yC
Cuts at: 550 1417 1665 2367 3863 550
Size: 867 248 702 1496 673
Fragments arranged by size:
1496 867 702 673 248
BsaWI w'CCGG_w
Cuts at: 294 733 1175 1696 2374 2648 2795 3206 294
Size: 439 442 521 678 274 147 411 1074
Fragments arranged by size:
1074 678 521 442 439 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 667 667
Size: 3986
BseYI C'CCAG_C
Cuts at: 262 1411 2169 2746 3242 262
Size: 1149 758 577 496 1006
Fragments arranged by size:
1149 1006 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 387
Size: 3986
BsiEI CG_ry'CG
Cuts at: 104 1528 1574 2782 104
Size: 1424 46 1208 1308
Fragments arranged by size:
1424 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 428 460 482 580 635 1000 1778 1968 2760
Size: 32 22 98 55 365 778 190 792
Cuts at: 2760 428
Size: 1654
Fragments arranged by size:
1654 792 778 365 190 98 55 32
22
Bsp1286I G_dGCh'C
Cuts at: 382 428 460 482 580 635 881 1000 1611
Size: 46 32 22 98 55 246 119 611
Cuts at: 1611 1704 1778 1968 2030 2760 382
Size: 93 74 190 62 730 1608
Fragments arranged by size:
1608 730 611 246 190 119 98 93
74 62 55 46 32 22
BspEI T'CCGG_A
Cuts at: 3206 3206
Size: 3986
BspHI T'CATG_A
Cuts at: 233 3162 233
Size: 2929 1057
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1036 1250 1552 1933 1036
Size: 214 302 381 3089
Fragments arranged by size:
3089 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2278 2332 3816 3981 50
Size: 2228 54 1484 165 55
Fragments arranged by size:
2228 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 596 1898 596
Size: 1302 2684
BsrFI r'CCGG_y
Cuts at: 1984 2165 2374 1984
Size: 181 209 3596
Fragments arranged by size:
3596 209 181
BssSI C'ACGA_G
Cuts at: 2257 2615 2257
Size: 358 3628
BstAPI GCAn_nnn'nTGC
Cuts at: 353 1363 353
Size: 1010 2976
BstBI TT'CG_AA
Cuts at: 2347 2347
Size: 3986
BstYI r'GATC_y
Cuts at: 1 128 350 1011 1478 1501 1836 2082 3094
Size: 127 222 661 467 23 335 246 1012
Cuts at: 3094 3180 3192 3538 1
Size: 86 12 346 449
Fragments arranged by size:
1012 661 467 449 346 335 246 222
127 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1936 2117 2280 1936
Size: 181 163 3642
Fragments arranged by size:
3642 181 163
BtsI GCAGTG_nn'
Cuts at: 118 399 451 1229 118
Size: 281 52 778 2875
Fragments arranged by size:
2875 778 281 52
DraI TTT'AAA
Cuts at: 985 985
Size: 3986
DraIII CAC_nnn'GTG
Cuts at: 423 423
Size: 3986
DrdI GACnn_nn'nnGTC
Cuts at: 1692 2550 1692
Size: 858 3128
EaeI y'GGCC_r
Cuts at: 719 1571 1745 2136 2163 3508 719
Size: 852 174 391 27 1345 1197
Fragments arranged by size:
1345 1197 852 391 174 27
EagI C'GGCC_G
Cuts at: 1571 1571
Size: 3986
EarI CTCTTCn'nnn_
Cuts at: 781 936 2009 2219 781
Size: 155 1073 210 2548
Fragments arranged by size:
2548 1073 210 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2194 2504 2650 3796 2194
Size: 310 146 1146 2384
Fragments arranged by size:
2384 1146 310 146
EcoICRI GAG'CTC
Cuts at: 458 480 998 458
Size: 22 518 3446
Fragments arranged by size:
3446 518 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 971 980 1811 2243 2990 3258 535
Size: 436 9 831 432 747 268 1263
Fragments arranged by size:
1263 831 747 436 432 268 9
EcoO109I rG'GnC_Cy
Cuts at: 1125 1125
Size: 3986
EcoRI G'AATT_C
Cuts at: 990 990
Size: 3986
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2005 2005
Size: 3986
FspI TGC'GCA
Cuts at: 1767 1767
Size: 3986
HaeII r_GCGC'y
Cuts at: 1420 1668 2690 3669 1420
Size: 248 1022 979 1737
Fragments arranged by size:
1737 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 893 913 925 945 953 985 1828
Size: 32 719 20 12 20 8 32 843
Cuts at: 1828 1858 1860 1890 142
Size: 30 2 30 2238
Fragments arranged by size:
2238 843 719 32 32 30 30 20
20 12 8 2
HincII GTy'rAC
Cuts at: 1025 1025
Size: 3986
KasI G'GCGC_C
Cuts at: 1416 1664 1416
Size: 248 3738
KpnI G_GTAC'C
Cuts at: 1006 1006
Size: 3986
MfeI C'AATT_G
Cuts at: 778 778
Size: 3986
MluI A'CGCG_T
Cuts at: 3419 3419
Size: 3986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 485 944 2657 2841 485
Size: 459 1713 184 1630
Fragments arranged by size:
1713 1630 459 184
MscI TGG'CCA
Cuts at: 721 1747 721
Size: 1026 2960
MslI CAynn'nnrTG
Cuts at: 112 610 902 1975 2102 112
Size: 498 292 1073 127 1996
Fragments arranged by size:
1996 1073 498 292 127
MspA1I CmG'CkG
Cuts at: 1097 1411 1771 2784 3029 1097
Size: 314 360 1013 245 2054
Fragments arranged by size:
2054 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2167 2167
Size: 3986
NarI GG'CG_CC
Cuts at: 1417 1665 1417
Size: 248 3738
NgoMIV G'CCGG_C
Cuts at: 2165 2165
Size: 3986
NspI r_CATG'y
Cuts at: 117 611 1039 1539 2446 117
Size: 494 428 500 907 1657
Fragments arranged by size:
1657 907 500 494 428
PciI A'CATG_T
Cuts at: 607 2442 607
Size: 1835 2151
PmeI GTTT'AAAC
Cuts at: 985 985
Size: 3986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3125 3157 3125
Size: 32 3954
PstI C_TGCA'G
Cuts at: 349 443 1033 1718 349
Size: 94 590 685 2617
Fragments arranged by size:
2617 685 590 94
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3986
PvuII CAG'CTG
Cuts at: 1411 1771 1411
Size: 360 3626
RsrII CG'GwC_CG
Cuts at: 2181 2181
Size: 3986
SacI G_AGCT'C
Cuts at: 460 482 1000 460
Size: 22 518 3446
Fragments arranged by size:
3446 518 22
SalI G'TCGA_C
Cuts at: 1023 1023
Size: 3986
SapI GCTCTTCn'nnn_
Cuts at: 2009 2219 2009
Size: 210 3776
SbfI CC_TGCA'GG
Cuts at: 1033 1033
Size: 3986
ScaI AGT'ACT
Cuts at: 3503 3503
Size: 3986
SfcI C'TryA_G
Cuts at: 33 171 345 439 1029 1714 2707 2898 33
Size: 138 174 94 590 685 993 191 1121
Fragments arranged by size:
1121 993 685 590 191 174 138 94
SfoI GGC'GCC
Cuts at: 1418 1666 1418
Size: 248 3738
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
SmaI CCC'GGG
Cuts at: 1008 1008
Size: 3986
SmlI C'TyrA_G
Cuts at: 793 1141 2056 2548 2810 793
Size: 348 915 492 262 1969
Fragments arranged by size:
1969 915 492 348 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3986
SphI G_CATG'C
Cuts at: 117 1039 1539 117
Size: 922 500 2564
Fragments arranged by size:
2564 922 500
SspI AAT'ATT
Cuts at: 852 852
Size: 3986
StuI AGG'CCT
Cuts at: 433 799 433
Size: 366 3620
StyI C'CwwG_G
Cuts at: 1120 1474 1120
Size: 354 3632
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1569 1702 2161 2370 1569
Size: 133 459 209 3185
Fragments arranged by size:
3185 459 209 133
TatI w'GTAC_w
Cuts at: 3501 3501
Size: 3986
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3503 3535 3503
Size: 32 3954
Tth111I GACn'n_nGTC
Cuts at: 1783 1783
Size: 3986
XbaI T'CTAG_A
Cuts at: 1017 1017
Size: 3986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 527 609 1481 527
Size: 82 872 3032
Fragments arranged by size:
3032 872 82
XmaI C'CCGG_G
Cuts at: 1006 1006
Size: 3986
XmnI GAAnn'nnTTC
Cuts at: 971 971
Size: 3986
ZraI GAC'GTC
Cuts at: 551 551
Size: 3986
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI