Restriction map for
pF1KB4809
(Circular) (Six-base) MAPSORT of: pF1KB4809.seq Check: 3933 from: 1 to: 5315
pF1KB4809 5315 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 401 401
Size: 5315
AccI GT'mk_AC
Cuts at: 1313 2353 1313
Size: 1040 4275
Acc65I G'GTAC_C
Cuts at: 860 2071 2331 860
Size: 1211 260 3844
Fragments arranged by size:
3844 1211 260
AclI AA'CG_TT
Cuts at: 5155 5155
Size: 5315
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 808 1300 1804 1873 3140 3572 3925 4587
Size: 261 492 504 69 1267 432 353 662
Cuts at: 4587 547
Size: 1275
Fragments arranged by size:
1275 1267 662 504 492 432 353 261
69
AflIII A'CryG_T
Cuts at: 1316 2667 4467 4748 1316
Size: 1351 1800 281 1883
Fragments arranged by size:
1883 1800 1351 281
AhdI GACnn_n'nnGTC
Cuts at: 540 1374 540
Size: 834 4481
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1054 1086 1054
Size: 32 5283
AlwNI CAG_nnn'CTG
Cuts at: 402 786 801 1278 1840 2620 4058 402
Size: 384 15 477 562 780 1438 1659
Fragments arranged by size:
1659 1438 780 562 477 384 15
ApaI G_GGCC'C
Cuts at: 1887 1887
Size: 5315
ApaLI G'TGCA_C
Cuts at: 4153 4153
Size: 5315
ApoI r'AATT_y
Cuts at: 1383 2319 4668 4924 1383
Size: 936 2349 256 1774
Fragments arranged by size:
2349 1774 936 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5315
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5315
AvaI C'yCGr_G
Cuts at: 670 2335 670
Size: 1665 3650
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 852 885 852
Size: 33 5282
BamHI G'GATC_C
Cuts at: 2340 2340
Size: 5315
BanI G'GyrC_C
Cuts at: 241 716 860 2071 2331 2745 2993 3028 241
Size: 475 144 1211 260 414 248 35 2528
Fragments arranged by size:
2528 1211 475 414 260 248 144 35
BanII G_rGCy'C
Cuts at: 1054 1189 1887 2329 3359 1054
Size: 135 698 442 1030 3010
Fragments arranged by size:
3010 1030 698 442 135
BbeI G_GCGC'C
Cuts at: 720 2749 2997 720
Size: 2029 248 3038
Fragments arranged by size:
3038 2029 248
BbvCI CC'TCA_GC
Cuts at: 1975 1975
Size: 5315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 555 589 661 695 555
Size: 34 72 34 5175
Fragments arranged by size:
5175 72 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3213 4269 5315 3213
Size: 1056 1046 3213
Fragments arranged by size:
3213 1056 1046
BclI T'GATC_A
Cuts at: 2835 2835
Size: 5315
BglI GCCn_nnn'nGGC
Cuts at: 674 2008 674
Size: 1334 3981
BglII A'GATC_T
Cuts at: 2830 4867 2830
Size: 2037 3278
BlpI GC'TnA_GC
Cuts at: 2122 2427 2122
Size: 305 5010
Bme1580I G_kGCm'C
Cuts at: 244 1147 1887 2940 3033 4157 244
Size: 903 740 1053 93 1124 1402
Fragments arranged by size:
1402 1124 1053 903 740 93
BmgBI CAC'GTC
Cuts at: 1319 1319
Size: 5315
BmrI ACTGGGnnnn_n'
Cuts at: 2527 2608 2943 5087 2527
Size: 81 335 2144 2755
Fragments arranged by size:
2755 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1128 3682 1128
Size: 2554 2761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 454 1902 1966 424
Size: 30 1448 64 3773
Fragments arranged by size:
3773 1448 64 30
Bpu10I CC'TnA_GC
Cuts at: 1975 1975
Size: 5315
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 475 2128 2290 2491 3370 4084 4382 268
Size: 207 1653 162 201 879 714 298 1201
Fragments arranged by size:
1653 1201 879 714 298 207 201 162
BsaI GGTCTCn'nnnn_
Cuts at: 5019 5019
Size: 5315
BsaAI yAC'GTr
Cuts at: 1728 2668 1728
Size: 940 4375
BsaBI GATnn'nnATC
Cuts at: 6 1660 2829 4866 6
Size: 1654 1169 2037 455
Fragments arranged by size:
2037 1654 1169 455
BsaHI Gr'CG_yC
Cuts at: 161 500 717 2746 2994 3696 5192 161
Size: 339 217 2029 248 702 1496 284
Fragments arranged by size:
2029 1496 702 339 284 248 217
BsaWI w'CCGG_w
Cuts at: 2068 2504 3025 3703 4114 4261 4535 2068
Size: 436 521 678 411 147 274 2848
Fragments arranged by size:
2848 678 521 436 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1054 1084 1394 1424 1900 1930 1054
Size: 30 310 30 476 30 4439
Fragments arranged by size:
4439 476 310 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 475 1075 1078 1081 1238 1699 2035 475
Size: 600 3 3 157 461 336 3755
Fragments arranged by size:
3755 600 461 336 157 3 3
BseYI C'CCAG_C
Cuts at: 712 1039 1271 1522 1589 2740 3498 4163 4571
Size: 327 232 251 67 1151 758 665 408
Cuts at: 4571 712
Size: 1456
Fragments arranged by size:
1456 1151 758 665 408 327 251 232
67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1708 1708
Size: 5315
BsiEI CG_ry'CG
Cuts at: 104 249 1587 2857 2903 4133 104
Size: 145 1338 1270 46 1230 1286
Fragments arranged by size:
1338 1286 1270 1230 145 46
BsiHKAI G_wGCw'C
Cuts at: 392 470 1679 2183 2225 2329 3107 3297 4157
Size: 78 1209 504 42 104 778 190 860
Cuts at: 4157 392
Size: 1550
Fragments arranged by size:
1550 1209 860 778 504 190 104 78
42
BsmI GAATG_Cn'
Cuts at: 515 515
Size: 5315
BsmBI CGTCTCn'nnnn_
Cuts at: 1591 1591
Size: 5315
Bsp1286I G_dGCh'C
Cuts at: 244 392 470 1054 1147 1189 1679 1887 2183
Size: 148 78 584 93 42 490 208 296
Cuts at: 2183 2225 2329 2940 3033 3107 3297 3359 4157
Size: 42 104 611 93 74 190 62 798
Cuts at: 4157 244
Size: 1402
Fragments arranged by size:
1402 798 611 584 490 296 208 190
148 104 93 93 78 74 62 42
42
BspHI T'CATG_A
Cuts at: 3747 3747
Size: 5315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 401 1244 2365 2579 2881 3262 401
Size: 843 1121 214 302 381 2454
Fragments arranged by size:
2454 1121 843 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1880 1916 3607 3661 5145 5310 50
Size: 1830 36 1691 54 1484 165 55
Fragments arranged by size:
1830 1691 1484 165 55 54 36
BsrDI GCAATG_nn'
Cuts at: 650 1163 3227 650
Size: 513 2064 2738
Fragments arranged by size:
2738 2064 513
BsrFI r'CCGG_y
Cuts at: 3313 3494 3313
Size: 181 5134
BsrGI T'GTAC_A
Cuts at: 371 371
Size: 5315
BssSI C'ACGA_G
Cuts at: 1894 3586 4294 1894
Size: 1692 708 2915
Fragments arranged by size:
2915 1692 708
BstAPI GCAn_nnn'nTGC
Cuts at: 801 1840 2250 2280 2692 801
Size: 1039 410 30 412 3424
Fragments arranged by size:
3424 1039 412 410 30
BstBI TT'CG_AA
Cuts at: 3676 3676
Size: 5315
BstEII G'GTnAC_C
Cuts at: 435 894 435
Size: 459 4856
BstXI CCAn_nnnn'nTGG
Cuts at: 1413 1413
Size: 5315
BstYI r'GATC_y
Cuts at: 1 333 557 2267 2340 2807 2830 3165 3411
Size: 332 224 1710 73 467 23 335 246
Cuts at: 3411 3717 3729 3815 4867 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1710 1052 467 449 335 332 306 246
224 86 73 23 12
BstZ17I GTA'TAC
Cuts at: 1314 1314
Size: 5315
BtgI C'CryG_G
Cuts at: 819 1406 1490 819
Size: 587 84 4644
Fragments arranged by size:
4644 587 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 558 1107 1947 2260 3265 3446 3609 558
Size: 549 840 313 1005 181 163 2264
Fragments arranged by size:
2264 1005 840 549 313 181 163
BtsI GCAGTG_nn'
Cuts at: 2558 2558
Size: 5315
DraI TTT'AAA
Cuts at: 2314 2314
Size: 5315
DraIII CAC_nnn'GTG
Cuts at: 552 2238 552
Size: 1686 3629
DrdI GACnn_nn'nnGTC
Cuts at: 481 877 1266 3021 4365 481
Size: 396 389 1755 1344 1431
Fragments arranged by size:
1755 1431 1344 396 389
EaeI y'GGCC_r
Cuts at: 338 359 1026 1415 1584 1703 1775 2900 3074
Size: 21 667 389 169 119 72 1125 174
Cuts at: 3074 3465 3492 4837 338
Size: 391 27 1345 816
Fragments arranged by size:
1345 1125 816 667 391 389 174 169
119 72 27 21
EagI C'GGCC_G
Cuts at: 1584 2900 1584
Size: 1316 3999
EarI CTCTTCn'nnn_
Cuts at: 2041 3338 3548 2041
Size: 1297 210 3808
Fragments arranged by size:
3808 1297 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1415 3523 4265 4411 5125 1415
Size: 2108 742 146 714 1605
Fragments arranged by size:
2108 1605 742 714 146
EcoICRI GAG'CTC
Cuts at: 2327 2327
Size: 5315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 454 547 808 1300 1804 1873 1902 1966
Size: 30 93 261 492 504 69 29 64
Cuts at: 1966 3140 3572 3925 4587 424
Size: 1174 432 353 662 1152
Fragments arranged by size:
1174 1152 662 504 492 432 353 261
93 69 64 30 29
EcoO109I rG'GnC_Cy
Cuts at: 354 1251 1368 1883 2025 2454 354
Size: 897 117 515 142 429 3215
Fragments arranged by size:
3215 897 515 429 142 117
EcoRI G'AATT_C
Cuts at: 2319 2319
Size: 5315
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2169 3334 2169
Size: 1165 4150
FspI TGC'GCA
Cuts at: 1960 3096 1960
Size: 1136 4179
FspAI rTGC'GCAy
Cuts at: 1960 1960
Size: 5315
HaeII r_GCGC'y
Cuts at: 210 720 2151 2749 2997 4227 4998 210
Size: 510 1431 598 248 1230 771 527
Fragments arranged by size:
1431 1230 771 598 527 510 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 318 350 526 558 1214 1246 1360 1392 1394
Size: 32 176 32 656 32 114 32 2
Cuts at: 1394 1426 1900 1932 2259 2291 3157 3187 3189
Size: 32 474 32 327 32 866 30 2
Cuts at: 3189 3219 318
Size: 30 2414
Fragments arranged by size:
2414 866 656 474 327 176 114 32
32 32 32 32 32 32 30 30
2 2
HincII GTy'rAC
Cuts at: 2354 2354
Size: 5315
KasI G'GCGC_C
Cuts at: 716 2745 2993 716
Size: 2029 248 3038
Fragments arranged by size:
3038 2029 248
KpnI G_GTAC'C
Cuts at: 864 2075 2335 864
Size: 1211 260 3844
Fragments arranged by size:
3844 1211 260
MluI A'CGCG_T
Cuts at: 4748 4748
Size: 5315
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 476 512 2033 4074 4258 476
Size: 36 1521 2041 184 1533
Fragments arranged by size:
2041 1533 1521 184 36
MscI TGG'CCA
Cuts at: 340 1705 1777 3076 340
Size: 1365 72 1299 2579
Fragments arranged by size:
2579 1365 1299 72
MslI CAynn'nnrTG
Cuts at: 655 1006 1150 1870 1933 3304 3431 655
Size: 351 144 720 63 1371 127 2539
Fragments arranged by size:
2539 1371 720 351 144 127 63
MspA1I CmG'CkG
Cuts at: 198 399 629 1342 1550 1589 1944 2426 2740
Size: 201 230 713 208 39 355 482 314
Cuts at: 2740 3100 3884 4129 198
Size: 360 784 245 1384
Fragments arranged by size:
1384 784 713 482 360 355 314 245
230 208 201 39
NaeI GCC'GGC
Cuts at: 3496 3496
Size: 5315
NarI GG'CG_CC
Cuts at: 717 2746 2994 717
Size: 2029 248 3038
Fragments arranged by size:
3038 2029 248
NcoI C'CATG_G
Cuts at: 1406 1490 1406
Size: 84 5231
NgoMIV G'CCGG_C
Cuts at: 3494 3494
Size: 5315
NspI r_CATG'y
Cuts at: 843 1714 2283 2368 2868 4471 843
Size: 871 569 85 500 1603 1687
Fragments arranged by size:
1687 1603 871 569 500 85
PasI CC'CwG_GG
Cuts at: 883 1255 883
Size: 372 4943
PciI A'CATG_T
Cuts at: 4467 4467
Size: 5315
PfoI T'CCnGG_A
Cuts at: 470 1378 470
Size: 908 4407
PmeI GTTT'AAAC
Cuts at: 2314 2314
Size: 5315
PmlI CAC'GTG
Cuts at: 1728 1728
Size: 5315
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1054 1086 3761 3793 1054
Size: 32 2675 32 2576
Fragments arranged by size:
2675 2576 32 32
PpuMI rG'GwC_Cy
Cuts at: 354 1251 1368 354
Size: 897 117 4301
Fragments arranged by size:
4301 897 117
PspOMI G'GGCC_C
Cuts at: 1883 1883
Size: 5315
PspXI vC'TCGA_Gb
Cuts at: 670 670
Size: 5315
PstI C_TGCA'G
Cuts at: 203 398 797 1845 1994 2246 2362 3047 203
Size: 195 399 1048 149 252 116 685 2471
Fragments arranged by size:
2471 1048 685 399 252 195 149 116
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5315
PvuII CAG'CTG
Cuts at: 198 399 629 1342 1944 2740 3100 198
Size: 201 230 713 602 796 360 2413
Fragments arranged by size:
2413 796 713 602 360 230 201
RsrII CG'GwC_CG
Cuts at: 588 3510 588
Size: 2922 2393
SacI G_AGCT'C
Cuts at: 2329 2329
Size: 5315
SalI G'TCGA_C
Cuts at: 2352 2352
Size: 5315
SanDI GG'GwC_CC
Cuts at: 1251 1368 1251
Size: 117 5198
SapI GCTCTTCn'nnn_
Cuts at: 2041 3338 3548 2041
Size: 1297 210 3808
Fragments arranged by size:
3808 1297 210
SbfI CC_TGCA'GG
Cuts at: 2362 2362
Size: 5315
ScaI AGT'ACT
Cuts at: 310 4832 310
Size: 4522 793
SexAI A'CCwGG_T
Cuts at: 548 548
Size: 5315
SfcI C'TryA_G
Cuts at: 33 138 199 394 793 1841 1990 2242 2358
Size: 105 61 195 399 1048 149 252 116
Cuts at: 2358 3043 4011 4202 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 1048 968 685 399 252 195 191
149 116 105 61
SfoI GGC'GCC
Cuts at: 718 2747 2995 718
Size: 2029 248 3038
Fragments arranged by size:
3038 2029 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5315
SmaI CCC'GGG
Cuts at: 2337 2337
Size: 5315
SmlI C'TyrA_G
Cuts at: 283 490 670 2143 2305 2470 3385 4099 4361
Size: 207 180 1473 162 165 915 714 262
Cuts at: 4361 283
Size: 1237
Fragments arranged by size:
1473 1237 915 714 262 207 180 165
162
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5315
SphI G_CATG'C
Cuts at: 2283 2368 2868 2283
Size: 85 500 4730
Fragments arranged by size:
4730 500 85
StyI C'CwwG_G
Cuts at: 416 596 1406 1490 1517 2449 2803 416
Size: 180 810 84 27 932 354 2928
Fragments arranged by size:
2928 932 810 354 180 84 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2898 3031 3490 3699 2898
Size: 133 459 209 4514
Fragments arranged by size:
4514 459 209 133
TatI w'GTAC_w
Cuts at: 308 371 4830 308
Size: 63 4459 793
Fragments arranged by size:
4459 793 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4832 4864 4832
Size: 32 5283
Tth111I GACn'n_nGTC
Cuts at: 874 3112 874
Size: 2238 3077
XbaI T'CTAG_A
Cuts at: 2346 2346
Size: 5315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 729 2810 729
Size: 2081 3234
XhoI C'TCGA_G
Cuts at: 670 670
Size: 5315
XmaI C'CCGG_G
Cuts at: 2335 2335
Size: 5315
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AlfI AscI AvrII
BbsI BfrBI BmtI BsiWI BspEI BssHII Bsu36I ClaI
CspCI EcoNI EcoRV FseI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PshAI PsiI
PsrI SacII SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XmnI ZraI