Restriction map for
pF1KB4784
(Circular) (Six-base) MAPSORT of: pF1KB4784.seq Check: 4445 from: 1 to: 3926
pF1KB4784 3926 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AatII G_ACGT'C
Cuts at: 890 890
Size: 3926
AccI GT'mk_AC
Cuts at: 890 964 890
Size: 74 3852
Acc65I G'GTAC_C
Cuts at: 942 942
Size: 3926
AclI AA'CG_TT
Cuts at: 3766 3766
Size: 3926
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1751 2183 2930 3198 1751
Size: 432 747 268 2479
Fragments arranged by size:
2479 747 432 268
AflIII A'CryG_T
Cuts at: 1278 2382 3359 1278
Size: 1104 977 1845
Fragments arranged by size:
1845 1104 977
AgeI A'CCGG_T
Cuts at: 641 2314 641
Size: 1673 2253
AlwNI CAG_nnn'CTG
Cuts at: 802 835 1231 2798 802
Size: 33 396 1567 1930
Fragments arranged by size:
1930 1567 396 33
ApaI G_GGCC'C
Cuts at: 663 918 663
Size: 255 3671
ApaLI G'TGCA_C
Cuts at: 2696 2696
Size: 3926
ApoI r'AATT_y
Cuts at: 930 3279 3535 930
Size: 2349 256 1321
Fragments arranged by size:
2349 1321 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3926
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
AvaI C'yCGr_G
Cuts at: 787 946 787
Size: 159 3767
BamHI G'GATC_C
Cuts at: 951 951
Size: 3926
BanI G'GyrC_C
Cuts at: 942 1356 1604 1639 942
Size: 414 248 35 3229
Fragments arranged by size:
3229 414 248 35
BanII G_rGCy'C
Cuts at: 284 663 918 940 1970 284
Size: 379 255 22 1030 2240
Fragments arranged by size:
2240 1030 379 255 22
BbeI G_GCGC'C
Cuts at: 1360 1608 1360
Size: 248 3678
BbsI GAAGACnn'nnnn_
Cuts at: 152 152
Size: 3926
BbvCI CC'TCA_GC
Cuts at: 715 715
Size: 3926
BciVI GTATCCnnnnn_n'
Cuts at: 1824 2585 3926 1824
Size: 761 1341 1824
Fragments arranged by size:
1824 1341 761
BclI T'GATC_A
Cuts at: 1446 1446
Size: 3926
BglI GCCn_nnn'nGGC
Cuts at: 604 604
Size: 3926
BglII A'GATC_T
Cuts at: 1441 3478 1441
Size: 2037 1889
BlpI GC'TnA_GC
Cuts at: 871 1038 871
Size: 167 3759
Bme1580I G_kGCm'C
Cuts at: 663 918 1551 1644 2700 663
Size: 255 633 93 1056 1889
Fragments arranged by size:
1889 1056 633 255 93
BmrI ACTGGGnnnn_n'
Cuts at: 1138 1219 1554 3698 1138
Size: 81 335 2144 1366
Fragments arranged by size:
2144 1366 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 3926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 325 316
Size: 9 3917
Bpu10I CC'TnA_GC
Cuts at: 715 715
Size: 3926
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 1102 1981 2473 2771 514
Size: 588 879 492 298 1669
Fragments arranged by size:
1669 879 588 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 183 3630 183
Size: 3447 479
BsaAI yAC'GTr
Cuts at: 1279 1279
Size: 3926
BsaBI GATnn'nnATC
Cuts at: 6 856 1440 3477 6
Size: 850 584 2037 455
Fragments arranged by size:
2037 850 584 455
BsaHI Gr'CG_yC
Cuts at: 149 887 1357 1605 2307 3803 149
Size: 738 470 248 702 1496 272
Fragments arranged by size:
1496 738 702 470 272 248
BsaWI w'CCGG_w
Cuts at: 641 1115 1636 2314 2588 2735 3146 641
Size: 474 521 678 274 147 411 1421
Fragments arranged by size:
1421 678 521 474 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 880 880
Size: 3926
BseYI C'CCAG_C
Cuts at: 248 1351 2109 2686 3182 248
Size: 1103 758 577 496 992
Fragments arranged by size:
1103 992 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 826
Size: 3926
BsiEI CG_ry'CG
Cuts at: 104 1468 1514 2722 104
Size: 1364 46 1208 1308
Fragments arranged by size:
1364 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 161 284 530 940 1718 1908 2700 161
Size: 123 246 410 778 190 792 1387
Fragments arranged by size:
1387 792 778 410 246 190 123
BsmI GAATG_Cn'
Cuts at: 798 798
Size: 3926
BsmBI CGTCTCn'nnnn_
Cuts at: 552 552
Size: 3926
Bsp1286I G_dGCh'C
Cuts at: 161 284 530 663 918 940 1551 1644 1718
Size: 123 246 133 255 22 611 93 74
Cuts at: 1718 1908 1970 2700 161
Size: 190 62 730 1387
Fragments arranged by size:
1387 730 611 255 246 190 133 123
93 74 62 22
BspEI T'CCGG_A
Cuts at: 3146 3146
Size: 3926
BspHI T'CATG_A
Cuts at: 3102 3102
Size: 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 379 641 976 1190 1492 1873 379
Size: 262 335 214 302 381 2432
Fragments arranged by size:
2432 381 335 302 262 214
BsrBI CCG'CTC
Cuts at: 50 875 2218 2272 3756 3921 50
Size: 825 1343 54 1484 165 55
Fragments arranged by size:
1484 1343 825 165 55 54
BsrDI GCAATG_nn'
Cuts at: 311 1838 311
Size: 1527 2399
BsrFI r'CCGG_y
Cuts at: 641 1924 2105 2314 641
Size: 1283 181 209 2253
Fragments arranged by size:
2253 1283 209 181
BssSI C'ACGA_G
Cuts at: 203 2197 2555 203
Size: 1994 358 1574
Fragments arranged by size:
1994 1574 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1303 1303
Size: 3926
BstBI TT'CG_AA
Cuts at: 2287 2287
Size: 3926
BstXI CCAn_nnnn'nTGG
Cuts at: 256 256
Size: 3926
BstYI r'GATC_y
Cuts at: 1 324 359 410 951 1418 1441 1776 2022
Size: 323 35 51 541 467 23 335 246
Cuts at: 2022 3034 3120 3132 3478 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 541 467 449 346 335 323 246
86 51 35 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3926
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1876 2057 2220 1876
Size: 181 163 3582
Fragments arranged by size:
3582 181 163
BtsI GCAGTG_nn'
Cuts at: 593 803 1169 593
Size: 210 366 3350
Fragments arranged by size:
3350 366 210
DraI TTT'AAA
Cuts at: 925 925
Size: 3926
DraIII CAC_nnn'GTG
Cuts at: 519 519
Size: 3926
DrdI GACnn_nn'nnGTC
Cuts at: 1632 2490 1632
Size: 858 3068
EaeI y'GGCC_r
Cuts at: 258 605 1511 1685 2076 2103 3448 258
Size: 347 906 174 391 27 1345 736
Fragments arranged by size:
1345 906 736 391 347 174 27
EagI C'GGCC_G
Cuts at: 1511 1511
Size: 3926
EarI CTCTTCn'nnn_
Cuts at: 595 1949 2159 595
Size: 1354 210 2362
Fragments arranged by size:
2362 1354 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2134 2444 2590 3736 2134
Size: 310 146 1146 2324
Fragments arranged by size:
2324 1146 310 146
EcoICRI GAG'CTC
Cuts at: 282 938 282
Size: 656 3270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 325 1751 2183 2930 3198 316
Size: 9 1426 432 747 268 1044
Fragments arranged by size:
1426 1044 747 432 268 9
EcoO109I rG'GnC_Cy
Cuts at: 225 368 711 732 915 1065 225
Size: 143 343 21 183 150 3086
Fragments arranged by size:
3086 343 183 150 143 21
EcoRI G'AATT_C
Cuts at: 930 930
Size: 3926
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1945 1945
Size: 3926
FspI TGC'GCA
Cuts at: 1707 1707
Size: 3926
HaeII r_GCGC'y
Cuts at: 1360 1608 2630 3609 1360
Size: 248 1022 979 1677
Fragments arranged by size:
1677 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 200 232 1768 1798 1800 1830 200
Size: 32 1536 30 2 30 2296
Fragments arranged by size:
2296 1536 32 30 30 2
HincII GTy'rAC
Cuts at: 965 965
Size: 3926
KasI G'GCGC_C
Cuts at: 1356 1604 1356
Size: 248 3678
KpnI G_GTAC'C
Cuts at: 946 946
Size: 3926
MluI A'CGCG_T
Cuts at: 3359 3359
Size: 3926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 302 2597 2781 302
Size: 2295 184 1447
Fragments arranged by size:
2295 1447 184
MscI TGG'CCA
Cuts at: 260 607 1687 260
Size: 347 1080 2499
Fragments arranged by size:
2499 1080 347
MslI CAynn'nnrTG
Cuts at: 1915 2042 1915
Size: 127 3799
MspA1I CmG'CkG
Cuts at: 909 1037 1351 1711 2724 2969 909
Size: 128 314 360 1013 245 1866
Fragments arranged by size:
1866 1013 360 314 245 128
NaeI GCC'GGC
Cuts at: 2107 2107
Size: 3926
NarI GG'CG_CC
Cuts at: 1357 1605 1357
Size: 248 3678
NcoI C'CATG_G
Cuts at: 107 107
Size: 3926
NgoMIV G'CCGG_C
Cuts at: 2105 2105
Size: 3926
NspI r_CATG'y
Cuts at: 256 979 1479 2386 256
Size: 723 500 907 1796
Fragments arranged by size:
1796 907 723 500
PasI CC'CwG_GG
Cuts at: 364 364
Size: 3926
PciI A'CATG_T
Cuts at: 2382 2382
Size: 3926
PfoI T'CCnGG_A
Cuts at: 752 752
Size: 3926
PmeI GTTT'AAAC
Cuts at: 925 925
Size: 3926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3065 3097 3065
Size: 32 3894
PpuMI rG'GwC_Cy
Cuts at: 368 711 732 368
Size: 343 21 3562
Fragments arranged by size:
3562 343 21
PspOMI G'GGCC_C
Cuts at: 659 914 659
Size: 255 3671
PstI C_TGCA'G
Cuts at: 973 1658 973
Size: 685 3241
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3926
PvuII CAG'CTG
Cuts at: 909 1351 1711 909
Size: 442 360 3124
Fragments arranged by size:
3124 442 360
RsrII CG'GwC_CG
Cuts at: 2121 2121
Size: 3926
SacI G_AGCT'C
Cuts at: 284 940 284
Size: 656 3270
SalI G'TCGA_C
Cuts at: 963 963
Size: 3926
SapI GCTCTTCn'nnn_
Cuts at: 1949 2159 1949
Size: 210 3716
SbfI CC_TGCA'GG
Cuts at: 973 973
Size: 3926
ScaI AGT'ACT
Cuts at: 3443 3443
Size: 3926
SexAI A'CCwGG_T
Cuts at: 515 515
Size: 3926
SfcI C'TryA_G
Cuts at: 33 491 969 1654 2647 2838 33
Size: 458 478 685 993 191 1121
Fragments arranged by size:
1121 993 685 478 458 191
SfoI GGC'GCC
Cuts at: 1358 1606 1358
Size: 248 3678
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
SmaI CCC'GGG
Cuts at: 948 948
Size: 3926
SmlI C'TyrA_G
Cuts at: 529 1081 1996 2488 2750 529
Size: 552 915 492 262 1705
Fragments arranged by size:
1705 915 552 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3926
SphI G_CATG'C
Cuts at: 979 1479 979
Size: 500 3426
StuI AGG'CCT
Cuts at: 173 537 173
Size: 364 3562
StyI C'CwwG_G
Cuts at: 107 900 1060 1414 107
Size: 793 160 354 2619
Fragments arranged by size:
2619 793 354 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 145 1509 1642 2101 2310 145
Size: 1364 133 459 209 1761
Fragments arranged by size:
1761 1364 459 209 133
TatI w'GTAC_w
Cuts at: 3441 3441
Size: 3926
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3443 3475 3443
Size: 32 3894
Tth111I GACn'n_nGTC
Cuts at: 1723 1723
Size: 3926
XbaI T'CTAG_A
Cuts at: 957 957
Size: 3926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 171 1421 171
Size: 1250 2676
XmaI C'CCGG_G
Cuts at: 946 946
Size: 3926
XmnI GAAnn'nnTTC
Cuts at: 584 584
Size: 3926
ZraI GAC'GTC
Cuts at: 888 888
Size: 3926
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI