Restriction map for
pF1KB4737
(Circular) (Six-base) MAPSORT of: pF1KB4737.seq Check: 1039 from: 1 to: 7211
pF1KB4737 7211 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 484 2977 484
Size: 2493 4718
AccI GT'mk_AC
Cuts at: 2757 3686 4249 2757
Size: 929 563 5719
Fragments arranged by size:
5719 929 563
Acc65I G'GTAC_C
Cuts at: 4227 4227
Size: 7211
AclI AA'CG_TT
Cuts at: 7051 7051
Size: 7211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3544 3584 5036 5468 6215 6483 3544
Size: 40 1452 432 747 268 4272
Fragments arranged by size:
4272 1452 747 432 268 40
AflIII A'CryG_T
Cuts at: 777 3780 4563 5667 6644 777
Size: 3003 783 1104 977 1344
Fragments arranged by size:
3003 1344 1104 977 783
AgeI A'CCGG_T
Cuts at: 5599 5599
Size: 7211
AleI CACnn'nnGTG
Cuts at: 3221 3221
Size: 7211
AlwNI CAG_nnn'CTG
Cuts at: 1678 1776 1957 4516 6083 1678
Size: 98 181 2559 1567 2806
Fragments arranged by size:
2806 2559 1567 181 98
ApaI G_GGCC'C
Cuts at: 208 1211 1363 2823 3155 3271 3726 208
Size: 1003 152 1460 332 116 455 3693
Fragments arranged by size:
3693 1460 1003 455 332 152 116
ApaLI G'TGCA_C
Cuts at: 2990 5981 2990
Size: 2991 4220
ApoI r'AATT_y
Cuts at: 4171 4215 6564 6820 4171
Size: 44 2349 256 4562
Fragments arranged by size:
4562 2349 256 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 7211
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7211
AvaI C'yCGr_G
Cuts at: 1661 2228 2739 4231 1661
Size: 567 511 1492 4641
Fragments arranged by size:
4641 1492 567 511
AvrII C'CTAG_G
Cuts at: 3262 3262
Size: 7211
BamHI G'GATC_C
Cuts at: 4236 4236
Size: 7211
BanI G'GyrC_C
Cuts at: 712 784 802 883 1027 1126 2152 4227 4641
Size: 72 18 81 144 99 1026 2075 414
Cuts at: 4641 4889 4924 712
Size: 248 35 2999
Fragments arranged by size:
2999 2075 1026 414 248 144 99 81
72 35 18
BanII G_rGCy'C
Cuts at: 208 429 753 1068 1196 1211 1363 2823 3140
Size: 221 324 315 128 15 152 1460 317
Cuts at: 3140 3155 3271 3726 3766 3991 4225 5255 208
Size: 15 116 455 40 225 234 1030 2164
Fragments arranged by size:
2164 1460 1030 455 324 317 315 234
225 221 152 128 116 40 15 15
BbeI G_GCGC'C
Cuts at: 4645 4893 4645
Size: 248 6963
BbsI GAAGACnn'nnnn_
Cuts at: 958 1318 1453 2695 3154 958
Size: 360 135 1242 459 5015
Fragments arranged by size:
5015 1242 459 360 135
BbvCI CC'TCA_GC
Cuts at: 4013 4013
Size: 7211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 7177
BciVI GTATCCnnnnn_n'
Cuts at: 117 3727 5109 5870 7211 117
Size: 3610 1382 761 1341 117
Fragments arranged by size:
3610 1382 1341 761 117
BclI T'GATC_A
Cuts at: 3225 4731 3225
Size: 1506 5705
BglI GCCn_nnn'nGGC
Cuts at: 257 521 872 980 1052 1520 1547 3113 3374
Size: 264 351 108 72 468 27 1566 261
Cuts at: 3374 257
Size: 4094
Fragments arranged by size:
4094 1566 468 351 264 261 108 72
27
BglII A'GATC_T
Cuts at: 4726 6763 4726
Size: 2037 5174
BlpI GC'TnA_GC
Cuts at: 112 3477 4323 112
Size: 3365 846 3000
Fragments arranged by size:
3365 3000 846
Bme1580I G_kGCm'C
Cuts at: 208 717 789 807 888 1211 1363 2002 2157
Size: 509 72 18 81 323 152 639 155
Cuts at: 2157 2823 2994 3155 3271 3726 4836 4929 5985
Size: 666 171 161 116 455 1110 93 1056
Cuts at: 5985 208
Size: 1434
Fragments arranged by size:
1434 1110 1056 666 639 509 455 323
171 161 155 152 116 93 81 72
18
BmgBI CAC'GTC
Cuts at: 778 3783 778
Size: 3005 4206
BmrI ACTGGGnnnn_n'
Cuts at: 1223 4186 4423 4504 4839 6983 1223
Size: 2963 237 81 335 2144 1451
Fragments arranged by size:
2963 2144 1451 335 237 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1215 5578 1215
Size: 4363 2848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 719 854 1028 1268 1352 1415 1577 1655
Size: 276 135 174 240 84 63 162 78
Cuts at: 1655 1745 2081 2189 2621 2672 2675 2873 3392
Size: 90 336 108 432 51 3 198 519
Cuts at: 3392 443
Size: 4262
Fragments arranged by size:
4262 519 432 336 276 240 198 174
162 135 108 90 84 78 63 51
3
Bpu10I CC'TnA_GC
Cuts at: 1697 4013 1697
Size: 2316 4895
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 3624 4387 5266 5758 6056 422
Size: 3202 763 879 492 298 1577
Fragments arranged by size:
3202 1577 879 763 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 328 1447 1834 1852 2095 2734 3541 6915 328
Size: 1119 387 18 243 639 807 3374 624
Fragments arranged by size:
3374 1119 807 639 624 387 243 18
BsaAI yAC'GTr
Cuts at: 229 4564 229
Size: 4335 2876
BsaBI GATnn'nnATC
Cuts at: 6 4725 6762 6
Size: 4719 2037 455
Fragments arranged by size:
4719 2037 455
BsaHI Gr'CG_yC
Cuts at: 1352 4642 4890 5592 7088 1352
Size: 3290 248 702 1496 1475
Fragments arranged by size:
3290 1496 1475 702 248
BsaWI w'CCGG_w
Cuts at: 4400 4921 5599 5873 6020 6431 4400
Size: 521 678 274 147 411 5180
Fragments arranged by size:
5180 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 273 303 3618 3648 273
Size: 30 3315 30 3836
Fragments arranged by size:
3836 3315 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 590 1580 2345 3964 590
Size: 990 765 1619 3837
Fragments arranged by size:
3837 1619 990 765
BseYI C'CCAG_C
Cuts at: 207 795 1060 1204 2194 2397 2545 3582 3741
Size: 588 265 144 990 203 148 1037 159
Cuts at: 3741 4636 5394 5971 6467 207
Size: 895 758 577 496 951
Fragments arranged by size:
1037 990 951 895 758 588 577 496
265 203 159 148 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 2633 437
Size: 2196 5015
BsiEI CG_ry'CG
Cuts at: 104 4753 4799 6007 104
Size: 4649 46 1208 1308
Fragments arranged by size:
4649 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 501 600 690 1662 1671 1698 2130 2139 2715
Size: 99 90 972 9 27 432 9 576
Cuts at: 2715 2868 2994 3237 3766 4225 5003 5193 5985
Size: 153 126 243 529 459 778 190 792
Cuts at: 5985 501
Size: 1727
Fragments arranged by size:
1727 972 792 778 576 529 459 432
243 190 153 126 99 90 27 9
9
BsmI GAATG_Cn'
Cuts at: 3116 3341 3116
Size: 225 6986
Bsp1286I G_dGCh'C
Cuts at: 208 429 501 600 690 717 753 789 807
Size: 221 72 99 90 27 36 36 18
Cuts at: 807 888 1068 1196 1211 1363 1662 1671 1698
Size: 81 180 128 15 152 299 9 27
Cuts at: 1698 2002 2130 2139 2157 2715 2823 2868 2994
Size: 304 128 9 18 558 108 45 126
Cuts at: 2994 3140 3155 3237 3271 3726 3766 3991 4225
Size: 146 15 82 34 455 40 225 234
Cuts at: 4225 4836 4929 5003 5193 5255 5985 208
Size: 611 93 74 190 62 730 1434
Fragments arranged by size:
1434 730 611 558 455 304 299 234
225 221 190 180 152 146 128 128
126 108 99 93 90 82 81 74
72 62 45 40 36 36 34 27
27 18 18 15 15 9 9
BspEI T'CCGG_A
Cuts at: 6431 6431
Size: 7211
BspHI T'CATG_A
Cuts at: 6387 6387
Size: 7211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 462 484 2977 3342 4016 4261 4475 4777 5158
Size: 22 2493 365 674 245 214 302 381
Cuts at: 5158 462
Size: 2515
Fragments arranged by size:
2515 2493 674 381 365 302 245 214
22
BsrBI CCG'CTC
Cuts at: 50 1178 5503 5557 7041 7206 50
Size: 1128 4325 54 1484 165 55
Fragments arranged by size:
4325 1484 1128 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2801 3932 5123 2801
Size: 1131 1191 4889
Fragments arranged by size:
4889 1191 1131
BsrFI r'CCGG_y
Cuts at: 943 1114 1276 1906 2806 5209 5390 5599 943
Size: 171 162 630 900 2403 181 209 2555
Fragments arranged by size:
2555 2403 900 630 209 181 171 162
BssSI C'ACGA_G
Cuts at: 500 599 1319 2129 2570 5482 5840 500
Size: 99 720 810 441 2912 358 1871
Fragments arranged by size:
2912 1871 810 720 441 358 99
BstAPI GCAn_nnn'nTGC
Cuts at: 3198 4588 3198
Size: 1390 5821
BstBI TT'CG_AA
Cuts at: 5572 5572
Size: 7211
BstEII G'GTnAC_C
Cuts at: 541 2107 2179 2575 2953 3214 3358 541
Size: 1566 72 396 378 261 144 4394
Fragments arranged by size:
4394 1566 396 378 261 144 72
BstXI CCAn_nnnn'nTGG
Cuts at: 3855 3855
Size: 7211
BstYI r'GATC_y
Cuts at: 1 1270 4236 4703 4726 5061 5307 6319 6405
Size: 1269 2966 467 23 335 246 1012 86
Cuts at: 6405 6417 6763 1
Size: 12 346 449
Fragments arranged by size:
2966 1269 1012 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 3687 3687
Size: 7211
Bsu36I CC'TnA_GG
Cuts at: 1788 2345 2696 3353 1788
Size: 557 351 657 5646
Fragments arranged by size:
5646 657 557 351
BtgI C'CryG_G
Cuts at: 753 807 833 951 1086 1833 2823 3011 3039
Size: 54 26 118 135 747 990 188 28
Cuts at: 3039 3219 3245 753
Size: 180 26 4719
Fragments arranged by size:
4719 990 747 188 180 135 118 54
28 26 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2936 5161 5342 5505 2936
Size: 2225 181 163 4642
Fragments arranged by size:
4642 2225 181 163
BtsI GCAGTG_nn'
Cuts at: 819 2000 3636 3918 4454 819
Size: 1181 1636 282 536 3576
Fragments arranged by size:
3576 1636 1181 536 282
DraI TTT'AAA
Cuts at: 4210 4210
Size: 7211
DraIII CAC_nnn'GTG
Cuts at: 415 595 1720 415
Size: 180 1125 5906
Fragments arranged by size:
5906 1125 180
DrdI GACnn_nn'nnGTC
Cuts at: 656 1457 2492 4917 5775 656
Size: 801 1035 2425 858 2092
Fragments arranged by size:
2425 2092 1035 858 801
EaeI y'GGCC_r
Cuts at: 2592 3887 4796 4970 5361 5388 6733 2592
Size: 1295 909 174 391 27 1345 3070
Fragments arranged by size:
3070 1345 1295 909 391 174 27
EagI C'GGCC_G
Cuts at: 4796 4796
Size: 7211
EarI CTCTTCn'nnn_
Cuts at: 5234 5444 5234
Size: 210 7001
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5419 5729 5875 7021 5419
Size: 310 146 1146 5609
Fragments arranged by size:
5609 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3764 4223 3764
Size: 459 6752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 719 854 1028 1268 1352 1415 1577 1655
Size: 276 135 174 240 84 63 162 78
Cuts at: 1655 1745 2081 2189 2621 2672 2675 2873 3392
Size: 90 336 108 432 51 3 198 519
Cuts at: 3392 3544 3584 5036 5468 6215 6483 443
Size: 152 40 1452 432 747 268 1171
Fragments arranged by size:
1452 1171 747 519 432 432 336 276
268 240 198 174 162 152 135 108
90 84 78 63 51 40 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3656 3967 3656
Size: 311 6900
EcoO109I rG'GnC_Cy
Cuts at: 204 250 286 397 433 451 829 838 1208
Size: 46 36 111 36 18 378 9 370
Cuts at: 1208 1216 1252 1288 1360 1405 1450 1504 1603
Size: 8 36 36 72 45 45 54 99
Cuts at: 1603 1675 1702 1747 1792 1936 1954 2341 2377
Size: 72 27 45 45 144 18 387 36
Cuts at: 2377 2449 2503 2647 2692 2819 3016 3097 3151
Size: 72 54 144 45 127 197 81 54
Cuts at: 3151 3250 3267 3268 3349 3367 4350 204
Size: 99 17 1 81 18 983 3065
Fragments arranged by size:
3065 983 387 378 370 197 144 144
127 111 99 99 81 81 72 72
72 54 54 54 46 45 45 45
45 45 36 36 36 36 36 27
18 18 18 17 9 8 1
EcoRI G'AATT_C
Cuts at: 4171 4215 4171
Size: 44 7167
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 982 2575 5230 982
Size: 1593 2655 2963
Fragments arranged by size:
2963 2655 1593
FspI TGC'GCA
Cuts at: 4992 4992
Size: 7211
HaeII r_GCGC'y
Cuts at: 4645 4893 5915 6894 4645
Size: 248 1022 979 4962
Fragments arranged by size:
4962 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1293 1325 1337 1369 3785 3817 4042 4074 5053
Size: 32 12 32 2416 32 225 32 979
Cuts at: 5053 5083 5085 5115 1293
Size: 30 2 30 3389
Fragments arranged by size:
3389 2416 979 225 32 32 32 32
30 30 12 2
HincII GTy'rAC
Cuts at: 2308 2859 2929 4250 2308
Size: 551 70 1321 5269
Fragments arranged by size:
5269 1321 551 70
KasI G'GCGC_C
Cuts at: 4641 4889 4641
Size: 248 6963
KpnI G_GTAC'C
Cuts at: 4231 4231
Size: 7211
MfeI C'AATT_G
Cuts at: 1544 1544
Size: 7211
MluI A'CGCG_T
Cuts at: 6644 6644
Size: 7211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 345 564 969 2364 3309 5882 6066 345
Size: 219 405 1395 945 2573 184 1490
Fragments arranged by size:
2573 1490 1395 945 405 219 184
MscI TGG'CCA
Cuts at: 3889 4972 3889
Size: 1083 6128
MslI CAynn'nnrTG
Cuts at: 3221 5200 5327 3221
Size: 1979 127 5105
Fragments arranged by size:
5105 1979 127
MspA1I CmG'CkG
Cuts at: 421 511 799 1088 1159 1492 1555 1753 1798
Size: 90 288 289 71 333 63 198 45
Cuts at: 1798 2401 4322 4636 4996 6009 6254 421
Size: 603 1921 314 360 1013 245 1378
Fragments arranged by size:
1921 1378 1013 603 360 333 314 289
288 245 198 90 71 63 45
NaeI GCC'GGC
Cuts at: 1278 2808 5392 1278
Size: 1530 2584 3097
Fragments arranged by size:
3097 2584 1530
NarI GG'CG_CC
Cuts at: 4642 4890 4642
Size: 248 6963
NcoI C'CATG_G
Cuts at: 1833 3219 1833
Size: 1386 5825
NgoMIV G'CCGG_C
Cuts at: 1276 2806 5390 1276
Size: 1530 2584 3097
Fragments arranged by size:
3097 2584 1530
NruI TCG'CGA
Cuts at: 2921 2921
Size: 7211
NspI r_CATG'y
Cuts at: 173 3596 4264 4764 5671 173
Size: 3423 668 500 907 1713
Fragments arranged by size:
3423 1713 907 668 500
PasI CC'CwG_GG
Cuts at: 1429 1951 2896 1429
Size: 522 945 5744
Fragments arranged by size:
5744 945 522
PciI A'CATG_T
Cuts at: 5667 5667
Size: 7211
PflMI CCAn_nnn'nTGG
Cuts at: 3752 3752
Size: 7211
PfoI T'CCnGG_A
Cuts at: 618 1005 1284 1329 1707 2850 3399 618
Size: 387 279 45 378 1143 549 4430
Fragments arranged by size:
4430 1143 549 387 378 279 45
PmeI GTTT'AAAC
Cuts at: 4210 4210
Size: 7211
PmlI CAC'GTG
Cuts at: 229 229
Size: 7211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2075 2107 6350 6382 2075
Size: 32 4243 32 2904
Fragments arranged by size:
4243 2904 32 32
PpuMI rG'GwC_Cy
Cuts at: 397 451 829 838 1216 1288 1405 1450 1504
Size: 54 378 9 378 72 117 45 54
Cuts at: 1504 1675 1702 1747 1792 1936 2503 2647 2692
Size: 171 27 45 45 144 567 144 45
Cuts at: 2692 3016 3097 3250 397
Size: 324 81 153 4358
Fragments arranged by size:
4358 567 378 378 324 171 153 144
144 117 81 72 54 54 45 45
45 45 27 9
PshAI GACnn'nnGTC
Cuts at: 2582 2582
Size: 7211
PspOMI G'GGCC_C
Cuts at: 204 1207 1359 2819 3151 3267 3722 204
Size: 1003 152 1460 332 116 455 3693
Fragments arranged by size:
3693 1460 1003 455 332 152 116
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1299 1331 2741 2773 3898 3930 1299
Size: 32 1410 32 1125 32 4580
Fragments arranged by size:
4580 1410 1125 32 32 32
PstI C_TGCA'G
Cuts at: 495 2988 4258 4943 495
Size: 2493 1270 685 2763
Fragments arranged by size:
2763 2493 1270 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7211
PvuII CAG'CTG
Cuts at: 421 511 799 1159 2401 4636 4996 421
Size: 90 288 360 1242 2235 360 2636
Fragments arranged by size:
2636 2235 1242 360 360 288 90
RsrII CG'GwC_CG
Cuts at: 5406 5406
Size: 7211
SacI G_AGCT'C
Cuts at: 3766 4225 3766
Size: 459 6752
SacII CC_GC'GG
Cuts at: 1089 1089
Size: 7211
SalI G'TCGA_C
Cuts at: 4248 4248
Size: 7211
SanDI GG'GwC_CC
Cuts at: 829 1450 1747 2647 829
Size: 621 297 900 5393
Fragments arranged by size:
5393 900 621 297
SapI GCTCTTCn'nnn_
Cuts at: 5234 5444 5234
Size: 210 7001
SbfI CC_TGCA'GG
Cuts at: 495 4258 495
Size: 3763 3448
ScaI AGT'ACT
Cuts at: 6728 6728
Size: 7211
SexAI A'CCwGG_T
Cuts at: 300 411 474 564 708 1977 2085 2310 2760
Size: 111 63 90 144 1269 108 225 450
Cuts at: 2760 2787 2994 300
Size: 27 207 4517
Fragments arranged by size:
4517 1269 450 225 207 144 111 108
90 63 27
SfcI C'TryA_G
Cuts at: 33 491 2147 2984 3456 3982 4254 4939 5932
Size: 458 1656 837 472 526 272 685 993
Cuts at: 5932 6123 33
Size: 191 1121
Fragments arranged by size:
1656 1121 993 837 685 526 472 458
272 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 980 1547 3374 980
Size: 567 1827 4817
Fragments arranged by size:
4817 1827 567
SfoI GGC'GCC
Cuts at: 4643 4891 4643
Size: 248 6963
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7211
SmaI CCC'GGG
Cuts at: 4233 4233
Size: 7211
SmlI C'TyrA_G
Cuts at: 437 2739 3603 4366 5281 5773 6035 437
Size: 2302 864 763 915 492 262 1613
Fragments arranged by size:
2302 1613 915 864 763 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7211
SphI G_CATG'C
Cuts at: 4264 4764 4264
Size: 500 6711
SspI AAT'ATT
Cuts at: 2967 2967
Size: 7211
StuI AGG'CCT
Cuts at: 974 1536 974
Size: 562 6649
StyI C'CwwG_G
Cuts at: 447 896 1031 1212 1833 2580 3219 3262 3363
Size: 449 135 181 621 747 639 43 101
Cuts at: 3363 3497 3657 3767 3848 4345 4699 447
Size: 134 160 110 81 497 354 2959
Fragments arranged by size:
2959 747 639 621 497 449 354 181
160 135 134 110 101 81 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2248 2770 3632 4794 4927 5386 5595 2248
Size: 522 862 1162 133 459 209 3864
Fragments arranged by size:
3864 1162 862 522 459 209 133
TatI w'GTAC_w
Cuts at: 6726 6726
Size: 7211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 273 305 1599 1631 1992 2024 2676 2708 3300
Size: 32 1294 32 361 32 652 32 592
Cuts at: 3300 3332 6728 6760 273
Size: 32 3396 32 724
Fragments arranged by size:
3396 1294 724 652 592 361 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5008 5008
Size: 7211
XbaI T'CTAG_A
Cuts at: 3571 4242 3571
Size: 671 6540
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 454 1219 1777 1840 2587 3370 3749 4706 454
Size: 765 558 63 747 783 379 957 2959
Fragments arranged by size:
2959 957 783 765 747 558 379 63
XhoI C'TCGA_G
Cuts at: 2739 2739
Size: 7211
XmaI C'CCGG_G
Cuts at: 4231 4231
Size: 7211
XmnI GAAnn'nnTTC
Cuts at: 2558 2558
Size: 7211
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI BaeI
BfrBI BmtI BsiWI BsmBI BsrGI BssHII ClaI CspCI
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NsiI PacI PsiI PspXI SgrAI SnaBI SrfI SwaI
ZraI