Restriction map for
pF1KB4735
(Circular) (Six-base) MAPSORT of: pF1KB4735.seq Check: 9038 from: 1 to: 4265
pF1KB4735 4265 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1303 1303
Size: 4265
Acc65I G'GTAC_C
Cuts at: 1281 1281
Size: 4265
AclI AA'CG_TT
Cuts at: 1259 4105 1259
Size: 2846 1419
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 957 2090 2522 2875 3537 105
Size: 852 1133 432 353 662 833
Fragments arranged by size:
1133 852 833 662 432 353
AflII C'TTAA_G
Cuts at: 292 292
Size: 4265
AflIII A'CryG_T
Cuts at: 1017 1617 3417 3698 1017
Size: 600 1800 281 1584
Fragments arranged by size:
1800 1584 600 281
AleI CACnn'nnGTG
Cuts at: 1156 1156
Size: 4265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 903 903
Size: 4265
AlwNI CAG_nnn'CTG
Cuts at: 1570 3008 1570
Size: 1438 2827
ApaLI G'TGCA_C
Cuts at: 3103 3103
Size: 4265
ApoI r'AATT_y
Cuts at: 574 997 1109 1132 1269 3618 3874 574
Size: 423 112 23 137 2349 256 965
Fragments arranged by size:
2349 965 423 256 137 112 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 4265
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
AvaI C'yCGr_G
Cuts at: 1285 1285
Size: 4265
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 280 313 280
Size: 33 4232
BamHI G'GATC_C
Cuts at: 699 1290 699
Size: 591 3674
BanI G'GyrC_C
Cuts at: 1281 1695 1943 1978 1281
Size: 414 248 35 3568
Fragments arranged by size:
3568 414 248 35
BanII G_rGCy'C
Cuts at: 394 967 1279 2309 394
Size: 573 312 1030 2350
Fragments arranged by size:
2350 1030 573 312
BbeI G_GCGC'C
Cuts at: 1699 1947 1699
Size: 248 4017
BciVI GTATCCnnnnn_n'
Cuts at: 2163 3219 4265 2163
Size: 1056 1046 2163
Fragments arranged by size:
2163 1056 1046
BclI T'GATC_A
Cuts at: 1056 1785 1056
Size: 729 3536
BglII A'GATC_T
Cuts at: 263 1780 3817 263
Size: 1517 2037 711
Fragments arranged by size:
2037 1517 711
BlpI GC'TnA_GC
Cuts at: 1377 1377
Size: 4265
Bme1580I G_kGCm'C
Cuts at: 984 1890 1983 3107 984
Size: 906 93 1124 2142
Fragments arranged by size:
2142 1124 906 93
BmgBI CAC'GTC
Cuts at: 900 900
Size: 4265
BmrI ACTGGGnnnn_n'
Cuts at: 1477 1558 1893 4037 1477
Size: 81 335 2144 1705
Fragments arranged by size:
2144 1705 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2632 2632
Size: 4265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 981 1228 408
Size: 573 247 3445
Fragments arranged by size:
3445 573 247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 1441 2320 3034 3332 225
Size: 1216 879 714 298 1158
Fragments arranged by size:
1216 1158 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3969 3969
Size: 4265
BsaAI yAC'GTr
Cuts at: 1020 1618 1020
Size: 598 3667
BsaBI GATnn'nnATC
Cuts at: 6 1779 3816 6
Size: 1773 2037 455
Fragments arranged by size:
2037 1773 455
BsaHI Gr'CG_yC
Cuts at: 1696 1944 2646 4142 1696
Size: 248 702 1496 1819
Fragments arranged by size:
1819 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1454 1975 2653 3064 3211 3485 1454
Size: 521 678 411 147 274 2234
Fragments arranged by size:
2234 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 127 142 157 172 127
Size: 15 15 15 4220
Fragments arranged by size:
4220 15 15 15
BseYI C'CCAG_C
Cuts at: 672 1099 1145 1690 2448 3113 3521 672
Size: 427 46 545 758 665 408 1416
Fragments arranged by size:
1416 758 665 545 427 408 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 381 646 381
Size: 265 4000
BsiEI CG_ry'CG
Cuts at: 104 1807 1853 3083 104
Size: 1703 46 1230 1286
Fragments arranged by size:
1703 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1208 1279 2057 2247 3107 1208
Size: 71 778 190 860 2366
Fragments arranged by size:
2366 860 778 190 71
BsmBI CGTCTCn'nnnn_
Cuts at: 527 527
Size: 4265
Bsp1286I G_dGCh'C
Cuts at: 394 967 984 1208 1279 1890 1983 2057 2247
Size: 573 17 224 71 611 93 74 190
Cuts at: 2247 2309 3107 394
Size: 62 798 1552
Fragments arranged by size:
1552 798 611 573 224 190 93 74
71 62 17
BspHI T'CATG_A
Cuts at: 560 2697 560
Size: 2137 2128
BspMI ACCTGCnnnn'nnnn_
Cuts at: 955 1315 1529 1831 2212 955
Size: 360 214 302 381 3008
Fragments arranged by size:
3008 381 360 302 214
BsrBI CCG'CTC
Cuts at: 50 2557 2611 4095 4260 50
Size: 2507 54 1484 165 55
Fragments arranged by size:
2507 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2177 2177
Size: 4265
BsrFI r'CCGG_y
Cuts at: 2263 2444 2263
Size: 181 4084
BsrGI T'GTAC_A
Cuts at: 406 602 406
Size: 196 4069
BssSI C'ACGA_G
Cuts at: 2536 3244 2536
Size: 708 3557
BstAPI GCAn_nnn'nTGC
Cuts at: 669 1642 669
Size: 973 3292
BstBI TT'CG_AA
Cuts at: 2626 2626
Size: 4265
BstEII G'GTnAC_C
Cuts at: 369 447 942 369
Size: 78 495 3692
Fragments arranged by size:
3692 495 78
BstXI CCAn_nnnn'nTGG
Cuts at: 429 429
Size: 4265
BstYI r'GATC_y
Cuts at: 1 263 699 1290 1757 1780 2115 2361 2667
Size: 262 436 591 467 23 335 246 306
Cuts at: 2667 2679 2765 3817 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 591 467 449 436 335 306 262
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2215 2396 2559 2215
Size: 181 163 3921
Fragments arranged by size:
3921 181 163
BtsI GCAGTG_nn'
Cuts at: 1037 1140 1508 1037
Size: 103 368 3794
Fragments arranged by size:
3794 368 103
DraI TTT'AAA
Cuts at: 1264 1264
Size: 4265
DraIII CAC_nnn'GTG
Cuts at: 404 404
Size: 4265
DrdI GACnn_nn'nnGTC
Cuts at: 802 1971 3315 802
Size: 1169 1344 1752
Fragments arranged by size:
1752 1344 1169
EaeI y'GGCC_r
Cuts at: 419 431 1091 1850 2024 2415 2442 3787 419
Size: 12 660 759 174 391 27 1345 897
Fragments arranged by size:
1345 897 759 660 391 174 27 12
EagI C'GGCC_G
Cuts at: 1850 1850
Size: 4265
EarI CTCTTCn'nnn_
Cuts at: 2288 2498 2288
Size: 210 4055
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2473 3215 3361 4075 2473
Size: 742 146 714 2663
Fragments arranged by size:
2663 742 714 146
EcoICRI GAG'CTC
Cuts at: 1277 1277
Size: 4265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 408 957 981 1228 2090 2522 2875 3537
Size: 303 549 24 247 862 432 353 662
Cuts at: 3537 105
Size: 833
Fragments arranged by size:
862 833 662 549 432 353 303 247
24
EcoNI CCTnn'n_nnAGG
Cuts at: 433 928 1093 433
Size: 495 165 3605
Fragments arranged by size:
3605 495 165
EcoO109I rG'GnC_Cy
Cuts at: 235 245 438 489 804 1404 235
Size: 10 193 51 315 600 3096
Fragments arranged by size:
3096 600 315 193 51 10
EcoRI G'AATT_C
Cuts at: 1269 1269
Size: 4265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 135 266 2284 135
Size: 131 2018 2116
Fragments arranged by size:
2116 2018 131
FspI TGC'GCA
Cuts at: 1084 2046 1084
Size: 962 3303
HaeII r_GCGC'y
Cuts at: 511 1699 1947 3177 3948 511
Size: 1188 248 1230 771 828
Fragments arranged by size:
1230 1188 828 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 2107 2137 2139 2169 142
Size: 32 1933 30 2 30 2238
Fragments arranged by size:
2238 1933 32 30 30 2
HincII GTy'rAC
Cuts at: 1304 1304
Size: 4265
KasI G'GCGC_C
Cuts at: 1695 1943 1695
Size: 248 4017
KpnI G_GTAC'C
Cuts at: 1285 1285
Size: 4265
MluI A'CGCG_T
Cuts at: 3698 3698
Size: 4265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 175 568 3024 3208 175
Size: 393 2456 184 1232
Fragments arranged by size:
2456 1232 393 184
MscI TGG'CCA
Cuts at: 421 2026 421
Size: 1605 2660
MslI CAynn'nnrTG
Cuts at: 1156 2254 2381 1156
Size: 1098 127 3040
Fragments arranged by size:
3040 1098 127
MspA1I CmG'CkG
Cuts at: 977 1376 1690 2050 2834 3079 977
Size: 399 314 360 784 245 2163
Fragments arranged by size:
2163 784 399 360 314 245
NaeI GCC'GGC
Cuts at: 2446 2446
Size: 4265
NarI GG'CG_CC
Cuts at: 1696 1944 1696
Size: 248 4017
NgoMIV G'CCGG_C
Cuts at: 2444 2444
Size: 4265
NspI r_CATG'y
Cuts at: 789 1318 1818 3421 789
Size: 529 500 1603 1633
Fragments arranged by size:
1633 1603 529 500
PasI CC'CwG_GG
Cuts at: 1187 1187
Size: 4265
PciI A'CATG_T
Cuts at: 3417 3417
Size: 4265
PflMI CCAn_nnn'nTGG
Cuts at: 990 990
Size: 4265
PfoI T'CCnGG_A
Cuts at: 385 893 958 385
Size: 508 65 3692
Fragments arranged by size:
3692 508 65
PmeI GTTT'AAAC
Cuts at: 1264 1264
Size: 4265
PmlI CAC'GTG
Cuts at: 1020 1020
Size: 4265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2711 2743 2711
Size: 32 4233
PpuMI rG'GwC_Cy
Cuts at: 235 438 489 804 235
Size: 203 51 315 3696
Fragments arranged by size:
3696 315 203 51
PsiI TTA'TAA
Cuts at: 173 173
Size: 4265
PstI C_TGCA'G
Cuts at: 1312 1997 1312
Size: 685 3580
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4265
PvuII CAG'CTG
Cuts at: 977 1690 2050 977
Size: 713 360 3192
Fragments arranged by size:
3192 713 360
RsrII CG'GwC_CG
Cuts at: 538 2460 538
Size: 1922 2343
SacI G_AGCT'C
Cuts at: 1279 1279
Size: 4265
SalI G'TCGA_C
Cuts at: 1302 1302
Size: 4265
SanDI GG'GwC_CC
Cuts at: 235 489 235
Size: 254 4011
SapI GCTCTTCn'nnn_
Cuts at: 2288 2498 2288
Size: 210 4055
SbfI CC_TGCA'GG
Cuts at: 1312 1312
Size: 4265
ScaI AGT'ACT
Cuts at: 3782 3782
Size: 4265
SexAI A'CCwGG_T
Cuts at: 373 373
Size: 4265
SfcI C'TryA_G
Cuts at: 33 936 1308 1993 2961 3152 33
Size: 903 372 685 968 191 1146
Fragments arranged by size:
1146 968 903 685 372 191
SfoI GGC'GCC
Cuts at: 1697 1945 1697
Size: 248 4017
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
SmaI CCC'GGG
Cuts at: 1287 1287
Size: 4265
SmlI C'TyrA_G
Cuts at: 240 292 1420 2335 3049 3311 240
Size: 52 1128 915 714 262 1194
Fragments arranged by size:
1194 1128 915 714 262 52
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4265
SphI G_CATG'C
Cuts at: 789 1318 1818 789
Size: 529 500 3236
Fragments arranged by size:
3236 529 500
SspI AAT'ATT
Cuts at: 649 649
Size: 4265
StyI C'CwwG_G
Cuts at: 628 736 1399 1753 628
Size: 108 663 354 3140
Fragments arranged by size:
3140 663 354 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 317 720 1848 1981 2440 2649 317
Size: 403 1128 133 459 209 1933
Fragments arranged by size:
1933 1128 459 403 209 133
TatI w'GTAC_w
Cuts at: 406 602 3780 406
Size: 196 3178 891
Fragments arranged by size:
3178 891 196
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3782 3814 3782
Size: 32 4233
Tth111I GACn'n_nGTC
Cuts at: 2062 2062
Size: 4265
XbaI T'CTAG_A
Cuts at: 1296 1296
Size: 4265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1189 1219 1760 1189
Size: 30 541 3694
Fragments arranged by size:
3694 541 30
XmaI C'CCGG_G
Cuts at: 1285 1285
Size: 4265
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AloI ApaI AscI
AvrII BbsI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BseRI BsiWI BsmI BspEI BssHII BstZ17I Bsu36I BtgI
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PshAI
PspOMI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI