Restriction map for
pF1KB4722
(Circular) (Six-base) MAPSORT of: pF1KB4722.seq Check: 2707 from: 1 to: 4103
pF1KB4722 4103 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 824 824
Size: 4103
AccI GT'mk_AC
Cuts at: 1141 1141
Size: 4103
Acc65I G'GTAC_C
Cuts at: 1119 1119
Size: 4103
AclI AA'CG_TT
Cuts at: 3943 3943
Size: 4103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 943 1928 2360 3107 3375 490
Size: 453 985 432 747 268 1218
Fragments arranged by size:
1218 985 747 453 432 268
AflIII A'CryG_T
Cuts at: 1455 2559 3536 1455
Size: 1104 977 2022
Fragments arranged by size:
2022 1104 977
AgeI A'CCGG_T
Cuts at: 2491 2491
Size: 4103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 196 196
Size: 4103
AlwNI CAG_nnn'CTG
Cuts at: 619 901 1408 2975 619
Size: 282 507 1567 1747
Fragments arranged by size:
1747 1567 507 282
ApaLI G'TGCA_C
Cuts at: 2873 2873
Size: 4103
ApoI r'AATT_y
Cuts at: 757 1107 3456 3712 757
Size: 350 2349 256 1148
Fragments arranged by size:
2349 1148 350 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4103
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
AvaI C'yCGr_G
Cuts at: 531 1123 531
Size: 592 3511
BamHI G'GATC_C
Cuts at: 1128 1128
Size: 4103
BanI G'GyrC_C
Cuts at: 836 1119 1533 1781 1816 836
Size: 283 414 248 35 3123
Fragments arranged by size:
3123 414 283 248 35
BanII G_rGCy'C
Cuts at: 1117 2147 1117
Size: 1030 3073
BbeI G_GCGC'C
Cuts at: 1537 1785 1537
Size: 248 3855
BciVI GTATCCnnnnn_n'
Cuts at: 2001 2762 4103 2001
Size: 761 1341 2001
Fragments arranged by size:
2001 1341 761
BclI T'GATC_A
Cuts at: 1623 1623
Size: 4103
BglII A'GATC_T
Cuts at: 445 1618 3655 445
Size: 1173 2037 893
Fragments arranged by size:
2037 1173 893
BlpI GC'TnA_GC
Cuts at: 1215 1215
Size: 4103
Bme1580I G_kGCm'C
Cuts at: 479 1728 1821 2877 479
Size: 1249 93 1056 1705
Fragments arranged by size:
1705 1249 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 607 1315 1396 1731 3875 607
Size: 708 81 335 2144 835
Fragments arranged by size:
2144 835 708 335 81
BmtI G_CTAG'C
Cuts at: 277 277
Size: 4103
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 1279 2158 2650 2948 571
Size: 708 879 492 298 1726
Fragments arranged by size:
1726 879 708 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3807 3807
Size: 4103
BsaAI yAC'GTr
Cuts at: 1456 1456
Size: 4103
BsaBI GATnn'nnATC
Cuts at: 6 1617 3654 6
Size: 1611 2037 455
Fragments arranged by size:
2037 1611 455
BsaHI Gr'CG_yC
Cuts at: 131 1534 1782 2484 3980 131
Size: 1403 248 702 1496 254
Fragments arranged by size:
1496 1403 702 254 248
BsaWI w'CCGG_w
Cuts at: 1292 1813 2491 2765 2912 3323 1292
Size: 521 678 274 147 411 2072
Fragments arranged by size:
2072 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 144 144
Size: 4103
BseYI C'CCAG_C
Cuts at: 1528 2286 2863 3359 1528
Size: 758 577 496 2272
Fragments arranged by size:
2272 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 997
Size: 4103
BsiEI CG_ry'CG
Cuts at: 104 1645 1691 2899 104
Size: 1541 46 1208 1308
Fragments arranged by size:
1541 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1117 1895 2085 2877 1117
Size: 778 190 792 2343
Fragments arranged by size:
2343 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 122 122
Size: 4103
Bsp1286I G_dGCh'C
Cuts at: 479 1117 1728 1821 1895 2085 2147 2877 479
Size: 638 611 93 74 190 62 730 1705
Fragments arranged by size:
1705 730 638 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3323 3323
Size: 4103
BspHI T'CATG_A
Cuts at: 3279 3279
Size: 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 824 1153 1367 1669 2050 824
Size: 329 214 302 381 2877
Fragments arranged by size:
2877 381 329 302 214
BsrBI CCG'CTC
Cuts at: 50 2395 2449 3933 4098 50
Size: 2345 54 1484 165 55
Fragments arranged by size:
2345 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2015 2015
Size: 4103
BsrFI r'CCGG_y
Cuts at: 2101 2282 2491 2101
Size: 181 209 3713
Fragments arranged by size:
3713 209 181
BssSI C'ACGA_G
Cuts at: 2374 2732 2374
Size: 358 3745
BstAPI GCAn_nnn'nTGC
Cuts at: 1480 1480
Size: 4103
BstBI TT'CG_AA
Cuts at: 2464 2464
Size: 4103
BstYI r'GATC_y
Cuts at: 1 445 535 1128 1595 1618 1953 2199 3211
Size: 444 90 593 467 23 335 246 1012
Cuts at: 3211 3297 3309 3655 1
Size: 86 12 346 449
Fragments arranged by size:
1012 593 467 449 444 346 335 246
90 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1026 2053 2234 2397 1026
Size: 1027 181 163 2732
Fragments arranged by size:
2732 1027 181 163
BtsI GCAGTG_nn'
Cuts at: 242 785 1346 242
Size: 543 561 2999
Fragments arranged by size:
2999 561 543
DraI TTT'AAA
Cuts at: 713 1102 713
Size: 389 3714
DrdI GACnn_nn'nnGTC
Cuts at: 1809 2667 1809
Size: 858 3245
EaeI y'GGCC_r
Cuts at: 161 1688 1862 2253 2280 3625 161
Size: 1527 174 391 27 1345 639
Fragments arranged by size:
1527 1345 639 391 174 27
EagI C'GGCC_G
Cuts at: 1688 1688
Size: 4103
EarI CTCTTCn'nnn_
Cuts at: 2126 2336 2126
Size: 210 3893
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 500 2311 2621 2767 3913 500
Size: 1811 310 146 1146 690
Fragments arranged by size:
1811 1146 690 310 146
EcoICRI GAG'CTC
Cuts at: 1115 1115
Size: 4103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 943 1928 2360 3107 3375 490
Size: 453 985 432 747 268 1218
Fragments arranged by size:
1218 985 747 453 432 268
EcoO109I rG'GnC_Cy
Cuts at: 644 1242 644
Size: 598 3505
EcoRI G'AATT_C
Cuts at: 1107 1107
Size: 4103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 4103
FspI TGC'GCA
Cuts at: 1884 1884
Size: 4103
HaeII r_GCGC'y
Cuts at: 1537 1785 2807 3786 1537
Size: 248 1022 979 1854
Fragments arranged by size:
1854 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 430 462 682 714 1945 1975 1977 2007 430
Size: 32 220 32 1231 30 2 30 2526
Fragments arranged by size:
2526 1231 220 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1142 1142
Size: 4103
KasI G'GCGC_C
Cuts at: 1533 1781 1533
Size: 248 3855
KpnI G_GTAC'C
Cuts at: 1123 1123
Size: 4103
MluI A'CGCG_T
Cuts at: 3536 3536
Size: 4103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 542 2774 2958 542
Size: 2232 184 1687
Fragments arranged by size:
2232 1687 184
MscI TGG'CCA
Cuts at: 1864 1864
Size: 4103
MslI CAynn'nnrTG
Cuts at: 863 998 2092 2219 863
Size: 135 1094 127 2747
Fragments arranged by size:
2747 1094 135 127
MspA1I CmG'CkG
Cuts at: 1214 1528 1888 2901 3146 1214
Size: 314 360 1013 245 2171
Fragments arranged by size:
2171 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2284 2284
Size: 4103
NarI GG'CG_CC
Cuts at: 1534 1782 1534
Size: 248 3855
NcoI C'CATG_G
Cuts at: 107 107
Size: 4103
NdeI CA'TA_TG
Cuts at: 318 318
Size: 4103
NgoMIV G'CCGG_C
Cuts at: 2282 2282
Size: 4103
NheI G'CTAG_C
Cuts at: 273 273
Size: 4103
NspI r_CATG'y
Cuts at: 1156 1656 2563 1156
Size: 500 907 2696
Fragments arranged by size:
2696 907 500
PciI A'CATG_T
Cuts at: 2559 2559
Size: 4103
PmeI GTTT'AAAC
Cuts at: 1102 1102
Size: 4103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 4071
PpuMI rG'GwC_Cy
Cuts at: 644 644
Size: 4103
PsiI TTA'TAA
Cuts at: 526 526
Size: 4103
PstI C_TGCA'G
Cuts at: 1150 1835 1150
Size: 685 3418
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4103
PvuII CAG'CTG
Cuts at: 1528 1888 1528
Size: 360 3743
RsrII CG'GwC_CG
Cuts at: 2298 2298
Size: 4103
SacI G_AGCT'C
Cuts at: 1117 1117
Size: 4103
SalI G'TCGA_C
Cuts at: 1140 1140
Size: 4103
SapI GCTCTTCn'nnn_
Cuts at: 2126 2336 2126
Size: 210 3893
SbfI CC_TGCA'GG
Cuts at: 1150 1150
Size: 4103
ScaI AGT'ACT
Cuts at: 3620 3620
Size: 4103
SexAI A'CCwGG_T
Cuts at: 938 938
Size: 4103
SfcI C'TryA_G
Cuts at: 33 678 1028 1146 1831 2824 3015 33
Size: 645 350 118 685 993 191 1121
Fragments arranged by size:
1121 993 685 645 350 191 118
SfoI GGC'GCC
Cuts at: 1535 1783 1535
Size: 248 3855
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
SmaI CCC'GGG
Cuts at: 1125 1125
Size: 4103
SmlI C'TyrA_G
Cuts at: 586 1258 2173 2665 2927 586
Size: 672 915 492 262 1762
Fragments arranged by size:
1762 915 672 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4103
SphI G_CATG'C
Cuts at: 1156 1656 1156
Size: 500 3603
SspI AAT'ATT
Cuts at: 874 874
Size: 4103
StyI C'CwwG_G
Cuts at: 107 116 1237 1591 107
Size: 9 1121 354 2619
Fragments arranged by size:
2619 1121 354 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1686 1819 2278 2487 1686
Size: 133 459 209 3302
Fragments arranged by size:
3302 459 209 133
TatI w'GTAC_w
Cuts at: 211 3618 211
Size: 3407 696
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3620 3652 3620
Size: 32 4071
Tth111I GACn'n_nGTC
Cuts at: 594 1900 594
Size: 1306 2797
XbaI T'CTAG_A
Cuts at: 1134 1134
Size: 4103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 141 1598 141
Size: 1457 2646
XmaI C'CCGG_G
Cuts at: 1123 1123
Size: 4103
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NspI PciI PmeI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BpmI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI